Download or read book Transformation Growth and the Cytoskeleton Tools to Study Oil Producing Algae written by Angelo Robert Collatos and published by . This book was released on 2013 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: With the current state of climate change and world peak oil on the horizon, it is important to focus our research efforts on alternative sources of energy. Ethanol obtained from the digestion of biomass (bioethanol) and oil harvesting from algae (biodiesel) are two promising fields of study for transportation fuel production. However, in their current state of development, neither option is capable of reasonably replacing the transportation fuel demand for this country. The land demand needed is too large for either process to become a viable option, albeit the land demand for biodiesel is considerably smaller than that of bioethanol. Therefore, when moving forward with alternative transportation fuel, harvesting oil from algae is a more promising option. Therefore, I investigated oil producing green algae to better understand algal growth, the algal cytoskeleton, and tried to establish a methodology to genetically manipulate algae. I developed a microgrowth assay in order to investigate algal growth and proliferation, while at the same time using considerably less material and space. This assay can directly monitor algal growth in response to media contents, and overcomes many of the limitations of existing microassays due to its use of solid media agar and fluorescent imaging. I also investigated algal genetic manipulation with the intention of creating a standard operating procedure, which could lead to further investigation of how to increase lipid output and increase lipid harvesting cycles through studying lipid production and cell division. Electroporation and PEG mediated transformation were the two chief methods investigated for nuclear transformation. Lastly, I performed an algal kinesin phylogenetic study to characterize the currently available algal kinesin superfamily, providing insight to proteins that are important for cell division as well as other functions within this superfamily. Kinesins 5, Kinesin 7s Class II and Class V, and Kinesin 14 Class I were identified to be important for algal cell division, while Kinesin 8, 12, 11, and some orphan kinesins will require further investigation due to their unknown plant function. Overall, this research provides a foundation for future algal studies required for optimal oil production necessary for a more sustainable future.
Download or read book Biofuels from Algae written by Kristian Spilling and published by Humana. This book was released on 2020-12-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a comprehensive overview of different ways to grow algae and the techniques used to start algal cultivation, monitor algal growth, environmental impact of growing algae, and various methods for characterizing the biomass. The chapters in this book discuss a range of topics such as step-by-step protocols on how to isolate and grow algae; descriptions on how to use fluorescence to estimate algal biomass and lipid content; and instructions on using advanced techniques to determine the carbohydrates and lipids in algal biomass. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Biofuels from Algae: Methods and Protocols is a valuable resource for novice and experienced researchers who want to learn more about this impactful and developing field.
Download or read book Development of Microalgae Tools and Techniques written by William Shahid Ansari and published by . This book was released on 2015 with total page 69 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microalgae are drawing increased attention from a variety of fields ranging from nutrition and health care to energy, and deservedly so. The potential of algae is almost unparalleled as a biomanufacturing platform. Microalgae can be used to produce complex human antibodies to target cancerous cells, or as crops for the production of high quality food, or even as sources of lipids for conversion into biofuels. They can grow to tremendous density in bioreactors, or be cultivated in open ponds where their yield per acre dominates that of higher plants. Research in microalgae needs to take it to the next step, to transform potential into reality and make production strains of microalgae into designer products that are custom tailored to suit the needs of the industry. The diversity of microalgae is enormous, and as a result, it is unlikely that the strains we have identified today will be the same we see as ideal production strains tomorrow. So in order to continue advancing the field, techniques for ideal cultivation, genetic modification, and metabolic engineering will need to be developed. This thesis work seeks to do just that by covering cultivation technology, metabolic engineering of lipids, and genetic modification of potential production strains. From a molecular perspective, a broad review of the current state of lipid metabolic engineering in both microalgae and higher plants is provided, covering both the biofuel relevant molecules as well as nutritionally relevant omega-3 fatty acids. New techniques for rapid interrogation of protein, lipid, and dry weight content in microalgae under either indoor or outdoor cultivation techniques are also described herein. These will enable more efficient harvesting and culturing techniques as well as more efficient use of fertilizers and nutrients in cultivation of microalgae. Proof of concept for custom tailoring lipid profile is demonstrated in the manipulation of Chlamydomonas reinhardtii lipid metabolism for the production of very long chain polyunsaturated fatty acids, which also provides insights into how changes in lipid profile can have unexpected effects and yield new insights on lipid metabolism. Finally, attempts to characterize and transform a variety of potential production species are covered and reveal challenges to the transformation of new microalgae species. Taken together, this work represents significant advances in the field with regard to both cultivation and transformation and metabolic engineering of microalgal species.
Download or read book Algae Biotechnology written by Faizal Bux and published by Springer. This book was released on 2016-03-09 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book examines the utilization of algae for the development of useful products and processes with the emphasis towards green technologies and processes, and the requirements to make these viable. Serving as a complete reference guide to the production of biofuels and other value added products from micro and macro algae, it covers various aspects of algal biotechnology from the basics to large scale cultivation, harvesting and processing for a variety of products. It is authored and edited by respected world experts in the field of algal biotechnology and provides the most up to date and cutting edge information on developments in the field. Over the past decade there has been substantial focus and related literature on the application of algal biomass for the generation of novel processes and products. ‘Algae Biotechnology: Products and Processes’ encompasses a holistic approach to critically evaluating developments in the field of algal biotechnology whilst taking into account recent advances and building on the body of knowledge. Aspects of the effects of harmful algae are also discussed, as well as the potential commercial application of algal biotechnology, the techno-economic feasibility of algal biodiesel production and the use of genetic and metabolic engineering for the improvement of yield. Other bioenergy sources such as alcohol fuels, aviation fuels, biohydrogen and biogas are also covered. This book is intended for postgraduates and researchers working in the biofuels and algal industry; it constitutes ideal reference material for both early stage and established researchers.
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 1876 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Download or read book Handbook of Microalgae Based Processes and Products written by Eduardo Jacob-Lopes and published by Academic Press. This book was released on 2020-07-23 with total page 938 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook of Microalgae-based Processes and Products provides a complete overview of all aspects involved in the production and utilization of microalgae resources at commercial scale. Divided into four parts (fundamentals, microalgae-based processes, microalgae-based products, and engineering approaches applied to microalgal processes and products), the book explores the microbiology and metabolic aspects of microalgae, microalgal production systems, wastewater treatment based in microalgae, CO2 capture using microalgae, microalgae harvesting techniques, and extraction and purification of biomolecules from microalgae. It covers the largest number of microalgal products of commercial relevance, including biogas, biodiesel, bioethanol, biohydrogen, single-cell protein, single-cell oil, biofertilizers, pigments, polyunsaturated fatty acids, bioactive proteins, peptides and amino acids, bioactive polysaccharides, sterols, bioplastics, UV-screening compounds, and volatile organic compounds. Moreover, it presents and discusses the available engineering tools applied to microalgae biotechnology, such as process integration, process intensification, and techno-economic analysis applied to microalgal processes and products, microalgal biorefineries, life cycle assessment, and exergy analysis of microalgae-based processes and products. The coverage of a broad range of potential microalgae processes and products in a single volume makes this handbook an indispensable reference for engineering researchers in academia and industry in the fields of bioenergy, sustainable development, and high-value compounds from biomass, as well as graduate students exploring those areas. Engineering professionals in bio-based industries will also find valuable information here when planning or implementing the use of microalgal technologies. Covers theoretical background information and results of recent research. Discusses all commercially relevant microalgae-based processes and products. Explores the main emerging engineering tools applied to microalgae processes, including techno-economic analysis, process integration, process intensification, life cycle assessment, and exergy analyses.
Download or read book Algae for Biofuels and Energy written by Michael A. Borowitzka and published by Springer Science & Business Media. This book was released on 2012-12-11 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microalgae are one of the most studied potential sources of biofuels and bioenergy. This book covers the key steps in the production of renewable biofuels from microalgae - strain selection, culture systems, inorganic carbon utilisation, lipid metabolism and quality, hydrogen production, genetic engineering, biomass harvesting, extraction. Greenhouse gas and techno-economic modelling are reviewed as is the 100 year history of microalgae as sources of biofuels and of commercial-scale microalgae culture. A summary of relevant basic standard methods used in the study of microalgae culture is provided. The book is intended for the expert and those starting work in the field.
Download or read book Bibliography of Agriculture written by and published by . This book was released on 1990 with total page 1120 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Plant Genomes written by Jean-Nicolas Volff and published by Karger Medical and Scientific Publishers. This book was released on 2008-01-01 with total page 155 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent major advances in the field of comparative genomics and cytogenomics of plants, particularly associated with the completion of ambitious genome projects, have uncovered astonishing facets of the architecture and evolutionary history of plant genomes. The aim of this book was to review these recent developments as well as their implications in our understanding of the mechanisms which drive plant diversity. New insights into the evolution of gene functions, gene families and genome size are presented, with particular emphasis on the evolutionary impact of polyploidization and transposable elements. Knowledge on the structure and evolution of plant sex chromosomes, centromeres and microRNAs is reviewed and updated. Taken together, the contributions by internationally recognized experts present a panoramic overview of the structural features and evolutionary dynamics of plant genomes.This volume of Genome Dynamics will provide researchers, teachers and students in the fields of biology and agronomy with a valuable source of current knowledge on plant genomes.
Download or read book Transgenic Microalgae as Green Cell Factories written by Rosa León and published by Springer Science & Business Media. This book was released on 2008-12-10 with total page 145 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microalgae have been largely commercialized as food and feed additives, and their potential as a source of high-added value compounds is well known. Yet, only a few species of microalgae have been genetically transformed with efficiency. A better understanding of the mechanisms that control the regulation of gene expression in eukaryotes is therefore needed. In this book a group of outstanding researchers working on different areas of microalgae biotechnology offer a global vision of the genetic manipulation of microalgae and their applications.
Download or read book Algae Based Polymers Blends and Composites written by Khalid Mahmood Zia and published by Elsevier. This book was released on 2017-06-19 with total page 740 pages. Available in PDF, EPUB and Kindle. Book excerpt: Algae Based Polymers, Blends, and Composites: Chemistry, Biotechnology and Material Sciences offers considerable detail on the origin of algae, extraction of useful metabolites and major compounds from algal bio-mass, and the production and future prospects of sustainable polymers derived from algae, blends of algae, and algae based composites. Characterization methods and processing techniques for algae-based polymers and composites are discussed in detail, enabling researchers to apply the latest techniques to their own work. The conversion of bio-mass into high value chemicals, energy, and materials has ample financial and ecological importance, particularly in the era of declining petroleum reserves and global warming. Algae are an important source of biomass since they flourish rapidly and can be cultivated almost everywhere. At present the majority of naturally produced algal biomass is an unused resource and normally is left to decompose. Similarly, the use of this enormous underexploited biomass is mainly limited to food consumption and as bio-fertilizer. However, there is an opportunity here for materials scientists to explore its potential as a feedstock for the production of sustainable materials. Provides detailed information on the extraction of useful compounds from algal biomass Highlights the development of a range of polymers, blends, and composites Includes coverage of characterization and processing techniques, enabling research scientists and engineers to apply the information to their own research and development Discusses potential applications and future prospects of algae-based biopolymers, giving the latest insight into the future of these sustainable materials
Download or read book Agrindex written by and published by . This book was released on 1993 with total page 820 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bibliography of Agriculture written by and published by . This book was released on 1981 with total page 1852 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Agrobacterium From Biology to Biotechnology written by Tzvi Tzfira and published by Springer Science & Business Media. This book was released on 2007-12-25 with total page 768 pages. Available in PDF, EPUB and Kindle. Book excerpt: Agrobacterium is a plant pathogen which causes the “crown-gall” disease, a neoplastic growth that results from the transfer of a well-defined DNA segment (“transferred DNA”, or “T-DNA”) from the bacterial Ti (tumor-inducing) plasmid to the host cell, its integration into the host genome, and the expression of oncogenes contained on the T-DNA. The molecular machinery, needed for T-DNA generation and transport into the host cell and encoded by a series of chromosomal (chv) and Ti-plasmid virulence (vir) genes, has been the subject of numerous studies over the past several decades. Today, Agrobacterium is the tool of choice for plant genetic engineering with an ever expanding host range that includes many commercially important crops, flowers, and tree species. Furthermore, its recent application for the genetic transformation of non-plant species, from yeast to cultivated mushrooms and even to human cells, promises this bacterium a unique place in the future of biotechnological applications. The book is a comprehensive volume describing Agrobacterium's biology, interactions with host species, and uses for genetic engineering.
Download or read book The Lichen Symbiosis written by Vernon Ahmadjian and published by John Wiley & Sons. This book was released on 1993-08-30 with total page 274 pages. Available in PDF, EPUB and Kindle. Book excerpt: Not only an invaluable reference to what is known about lichen bionts and their interactions but also a guide to future studies. Compares various aspects of lichen-forming bionts with those of other fungi, algae and cyanobacteria. Features in-depth descriptions of culture methods. Includes over 1000 references representing a selective sampling in such subjects as air pollution, photosynthesis and respiration.
Download or read book Algae Source to Treatment written by American Water Works Association and published by American Water Works Association. This book was released on 2010-12-01 with total page 465 pages. Available in PDF, EPUB and Kindle. Book excerpt: AWWA Manual of Water Supply Practice M57 provides all the information required by water treatment professionals to understand and mitigate problems caused by algae in source waters, such as tastes and odors, biofouling, and toxin production. With more than 450 pages and hundreds of photos and illustrations, the manual is a comprehensive reference for identifying and treating algae from drinking water sources.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1997 with total page 1856 pages. Available in PDF, EPUB and Kindle. Book excerpt:
