Download or read book Protein Secretion in Bacteria written by Maria Sandkvist and published by John Wiley & Sons. This book was released on 2020-07-02 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein transport into and across membranes is a fundamental process in bacteria that touches upon and unites many areas of microbiology, including bacterial cell physiology, adhesion and motility, nutrient scavenging, intrabacterial signaling and social behavior, toxin deployment, interbacterial antagonism and collaboration, host invasion and disruption, and immune evasion. A broad repertoire of mechanisms and macromolecular machines are required to deliver protein substrates across bacterial cell membranes for intended effects. Some machines are common to most, if not all bacteria, whereas others are specific to Gram-negative or Gram-positive species or species with unique cell envelope properties such as members of Actinobacteria and Spirochetes. Protein Secretion in Bacteria, authored and edited by an international team of experts, draws together the many distinct functions and mechanisms involved in protein translocation in one concise tome. This comprehensive book presents updated information on all aspects of bacterial protein secretion encompassing: Individual secretory systems–Sec, Tat, and T1SS through the newly discovered T9SS Mechanisms, structures, and functions of bacterial secretion systems Lipoprotein sorting pathways, outer membrane vesicles, and the sortase system Structures and roles of surface organelles, including flagella, pili, and curli Emerging technologies and translational implications Protein Secretion in Bacteria serves as both an introductory guide for students and postdocs and a ready reference for seasoned researchers whose work touches on protein export and secretion. This volume synthesizes the diversity of mechanisms of bacterial secretion across the microbial world into a digestible resource to stimulate new research, inspire continued identification and characterization of novel systems, and bring about new ways to manipulate these systems for biotechnological, preventative, and therapeutic applications.
Download or read book Bacterial Protein Secretion Systems written by Laure Journet and published by Humana Press. This book was released on 2017-07-01 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but are also of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.
Download or read book The Bacterial Flagellum written by Tohru Minamino and published by Humana. This book was released on 2018-06-21 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume examines the structure and dynamics of the bacterial flagellum using bacterial genetics, molecular biology, biochemistry, structural biology, biophysics, cell biology, and molecular dynamics simulation. The chapters are divided into 4 parts: Part I describes flagellar type III protein exports, assembly, and gene regulation in S. enterica; Part II explains how to isolate the flagella from the bacterial cell bodies, and further explains how to conduct high-resolution structural and functional analyses of the flagellar motor; Part III talks about how to measure flagellar motor rotation over a wide range of external load, how to measure ion motive force across the cytoplasmic membrane, and how to measure dynamic properties of the flagellar motor proteins by fluorescence microscopy with single molecule precision; and Part IV explores the structure and function of Spirochetal, Vibrio, Shewanella, and Magnetococcus flagellar motors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, The Bacterial Flagellum: Methods and Protocols aims to provide valuable and vital research to aid in the investigation of the bacterial flagellum resulting from various bacterial species.
Download or read book The Comprehensive Sourcebook of Bacterial Protein Toxins written by Joseph E. Alouf and published by Elsevier. This book was released on 2005-12-20 with total page 1072 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the major achievements and discoveries relevant to bacterial protein toxins since the turn of the new century illustrated by the discovery of more than fifty novel toxins (many of them identified through genome screening). The establishment of the three-dimensional crystal structure of more than 20 toxins during the same period offers deeper knowledge of structure-activity relationships and provides a framework to understand how toxins recognize receptors, penetrate membranes and interact with and modify intracellular substrates. - Edited by two of the most highly regarded experts in the field from the Institut Pasteur, France - 14 brand new chapters dedicated to coverage of historical and general aspects of toxinology - Includes the major toxins of both basic and clinical interest are described in depth - Details applied aspects of toxins such as therapy, vaccinology, and toolkits in cell biology - Evolutionary and functional aspects of bacterial toxins evaluated and summarized - Toxin applications in cell biology presented - Therapy (cancer therapy, dystonias) discussed - Vaccines (native and genetically engineered vaccines) featured - Toxins discussed as biological weapons, comprising chapters on anthrax, diphtheria, ricin etc.
Download or read book Prokaryotic Metabolism and Physiology written by Byung Hong Kim and published by Cambridge University Press. This book was released on 2019-05-16 with total page 509 pages. Available in PDF, EPUB and Kindle. Book excerpt: Extensive and up-to-date review of key metabolic processes in bacteria and archaea and how metabolism is regulated under various conditions.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Fungi written by Michael J. Carlile and published by Gulf Professional Publishing. This book was released on 2001-01-09 with total page 814 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition of The Fungi provides a comprehensive introduction to the importance of fungi in the natural world and in practical applications, from a microbiological perspective.
Download or read book Cyanobacteria Biotechnology written by Paul Hudson and published by John Wiley & Sons. This book was released on 2021-04-20 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Unites a biological and a biotechnological perspective on cyanobacteria, and includes the industrial aspects and applications of cyanobacteria Cyanobacteria Biotechnology offers a guide to the interesting and useful features of cyanobacteria metabolism that keeps true to a biotechnology vision. In one volume the book brings together both biology and biotechnology to illuminate the core acpects and principles of cyanobacteria metabolism. Designed to offer a practical approach to the metabolic engineering of cyanobacteria, the book contains relevant examples of how this metabolic "module" is currently being engineered and how it could be engineered in the future. The author includes information on the requirements and real-world experiences of the industrial applications of cyanobacteria. This important book: Brings together biology and biotechnology in order to gain insight into the industrial relevant topic of cyanobacteria Introduces the key aspects of the metabolism of cyanobacteria Presents a grounded, practical approach to the metabolic engineering of cyanobacteria Offers an analysis of the requirements and experiences for industrial cyanobacteria Provides a framework for readers to design their own processes Written for biotechnologists, microbiologists, biologists, biochemists, Cyanobacteria Biotechnology provides a systematic and clear volume that brings together the biological and biotechnological perspective on cyanobacteria.
Download or read book ABC Proteins written by I Barry Holland and published by Elsevier. This book was released on 2003-01-07 with total page 672 pages. Available in PDF, EPUB and Kindle. Book excerpt: ABC Proteins is an in-depth, up-to-date analysis of all that is known about the subject to date. It discusses and compares evolution, biology and mechanism of action of all known ABC proteins, including the first structural studies as well as clinical implications. It will be useful to anyone trying to stay abreast of the latest findings. This book is sure to become a classic and will regularly be updated. - Phylogeny and Evoloution of ABC Transporters - Fundamental Aspects of the Mechanism of Action of ABC Transporters - Prokaryote ABC Transporters - Non-Mammalian Transporters - Multidrug Transporters - ABC Transporters, Physiological Roles and Human Disease - Full color throughout
Download or read book Microbial Transport Systems written by Günther Winkelmann and published by Wiley-VCH Verlag GmbH. This book was released on 2001 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transport of molecules across the cell membrane is a fundamental process of all living organisms. It is essential for understanding growth, development, nutrition as well as uptake and excretion of exogenous or synthesized molecules. Microbes respresent general and basic functional systems where many transport processes have been studied on a molecular basis. Knowledge of the microbial transport processes will provide new perspectives to treatments by inhibitors, drugs, antibiotics, vitamins, growth promotion compounds, activators and toxic compunds of various kinds.
Download or read book Protein Secretion and Export in Bacteria written by Henry C. Wu and published by Springer. This book was released on 2011-11-17 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last decade has witnessed rapid progress in our under standing of the mechanisms of protein export and secretion in both prokaryotic and eukaryotic cells. Studies of protein secretion across the membranes of the rough endoplasmic reticulum have led to the formulation of the now-classic signal hypothesis, which has stimulated many discussions and new ideas, and the identification of the signal recogni tion particle as an organelle in the initiation of the export process. However, more recent work pertaining to intrage nic information related to targeting specific proteins for either secretion or membrane localization, the energetics of protein secretion, the timing of synthesis versus the initia tion of export, structural requirements for the processing of precursor proteins, and the identification of the proces sing enzymes (signal peptidases), has been the result of a combined biochemical and genetic approach to the study of protein localization in bacteria. While reviews on the biochemistry and genetics of pro tein secretion have appeared frequently in recent years, this book attempts to summarize the current status and the future perspectives of this rapidly moving field in a single volume. Topics covered in this book include the genetics of protein secretion in E. coli, biochemical analysis of pro tein export in vitro, signal peptidases, excretion of colicins and hemolysin in E. coli, protein secretion in Bacillus, and protein secretion cloning vectors.
Download or read book Bacterial Protein Toxins written by K. Aktories and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 713 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years remarkable progress has been accomplished with respect to our knowledge about bacterial protein toxins. This refers especially to structural aspects of protein toxins but also holds true for genetics, molecular biology and biochemical mechanisms underlying the action of toxins. This volume covers the very current and exciting aspects of up-to-date bacterial toxicology and comprehensively reviews the most important bacterial protein toxins such as the intracellular acting toxins which exhibit enzyme activity, as well as those toxins that interact with cell plasma membranes by damaging the membranes (pore formation) or stimulating cell receptors (superantigens). This is the most current reference work on these important bacterial protein toxins, which are presented from the point of view of different disciplines such as pharmacology, microbiology, cell biology and protein chemistry.
Download or read book Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria written by Frans J. de Bruijn and published by John Wiley & Sons. This book was released on 2016-07-13 with total page 1472 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacteria in various habitats are subject to continuously changing environmental conditions, such as nutrient deprivation, heat and cold stress, UV radiation, oxidative stress, dessication, acid stress, nitrosative stress, cell envelope stress, heavy metal exposure, osmotic stress, and others. In order to survive, they have to respond to these conditions by adapting their physiology through sometimes drastic changes in gene expression. In addition they may adapt by changing their morphology, forming biofilms, fruiting bodies or spores, filaments, Viable But Not Culturable (VBNC) cells or moving away from stress compounds via chemotaxis. Changes in gene expression constitute the main component of the bacterial response to stress and environmental changes, and involve a myriad of different mechanisms, including (alternative) sigma factors, bi- or tri-component regulatory systems, small non-coding RNA’s, chaperones, CHRIS-Cas systems, DNA repair, toxin-antitoxin systems, the stringent response, efflux pumps, alarmones, and modulation of the cell envelope or membranes, to name a few. Many regulatory elements are conserved in different bacteria; however there are endless variations on the theme and novel elements of gene regulation in bacteria inhabiting particular environments are constantly being discovered. Especially in (pathogenic) bacteria colonizing the human body a plethora of bacterial responses to innate stresses such as pH, reactive nitrogen and oxygen species and antibiotic stress are being described. An attempt is made to not only cover model systems but give a broad overview of the stress-responsive regulatory systems in a variety of bacteria, including medically important bacteria, where elucidation of certain aspects of these systems could lead to treatment strategies of the pathogens. Many of the regulatory systems being uncovered are specific, but there is also considerable “cross-talk” between different circuits. Stress and Environmental Regulation of Gene Expression and Adaptation in Bacteria is a comprehensive two-volume work bringing together both review and original research articles on key topics in stress and environmental control of gene expression in bacteria. Volume One contains key overview chapters, as well as content on one/two/three component regulatory systems and stress responses, sigma factors and stress responses, small non-coding RNAs and stress responses, toxin-antitoxin systems and stress responses, stringent response to stress, responses to UV irradiation, SOS and double stranded systems repair systems and stress, adaptation to both oxidative and osmotic stress, and desiccation tolerance and drought stress. Volume Two covers heat shock responses, chaperonins and stress, cold shock responses, adaptation to acid stress, nitrosative stress, and envelope stress, as well as iron homeostasis, metal resistance, quorum sensing, chemotaxis and biofilm formation, and viable but not culturable (VBNC) cells. Covering the full breadth of current stress and environmental control of gene expression studies and expanding it towards future advances in the field, these two volumes are a one-stop reference for (non) medical molecular geneticists interested in gene regulation under stress.
Download or read book Protein Secretion and Export in Bacteria written by Henry C. Wu and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 211 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last decade has witnessed rapid progress in our under standing of the mechanisms of protein export and secretion in both prokaryotic and eukaryotic cells. Studies of protein secretion across the membranes of the rough endoplasmic reticulum have led to the formulation of the now-classic signal hypothesis, which has stimulated many discussions and new ideas, and the identification of the signal recogni tion particle as an organelle in the initiation of the export process. However, more recent work pertaining to intrage nic information related to targeting specific proteins for either secretion or membrane localization, the energetics of protein secretion, the timing of synthesis versus the initia tion of export, structural requirements for the processing of precursor proteins, and the identification of the proces sing enzymes (signal peptidases), has been the result of a combined biochemical and genetic approach to the study of protein localization in bacteria. While reviews on the biochemistry and genetics of pro tein secretion have appeared frequently in recent years, this book attempts to summarize the current status and the future perspectives of this rapidly moving field in a single volume. Topics covered in this book include the genetics of protein secretion in E. coli, biochemical analysis of pro tein export in vitro, signal peptidases, excretion of colicins and hemolysin in E. coli, protein secretion in Bacillus, and protein secretion cloning vectors.
Download or read book Fundamentals of Bacterial Physiology and Metabolism written by Rani Gupta and published by Springer Nature. This book was released on 2021-04-20 with total page 670 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides useful information on microbial physiology and metabolism. The key aspects covered are prokaryotic diversity, growth physiology, basic metabolic pathways and their regulation, metabolic diversity with details of various unique pathways. Another focus area is stress physiology with details on varying environmental stresses, signal transduction, adaptation and survival. For instructional purposes, the book provides case studies, interesting facts, techniques etc. which help in showcasing the inter-disciplinary nature and bridge the gap between various aspects of applied microbiology.
Download or read book Evolutionary Genomics written by Maria Anisimova and published by Humana Press. This book was released on 2012-03-08 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt: Together with early theoretical work in population genetics, the debate on sources of genetic makeup initiated by proponents of the neutral theory made a solid contribution to the spectacular growth in statistical methodologies for molecular evolution. Evolutionary Genomics: Statistical and Computational Methods is intended to bring together the more recent developments in the statistical methodology and the challenges that followed as a result of rapidly improving sequencing technologies. Presented by top scientists from a variety of disciplines, the collection includes a wide spectrum of articles encompassing theoretical works and hands-on tutorials, as well as many reviews with key biological insight. Volume 2 begins with phylogenomics and continues with in-depth coverage of natural selection, recombination, and genomic innovation. The remaining chapters treat topics of more recent interest, including population genomics, -omics studies, and computational issues related to the handling of large-scale genomic data. Written in the highly successful Methods in Molecular BiologyTM series format, this work provides the kind of advice on methodology and implementation that is crucial for getting ahead in genomic data analyses. Comprehensive and cutting-edge, Evolutionary Genomics: Statistical and Computational Methods is a treasure chest of state-of the-art methods to study genomic and omics data, certain to inspire both young and experienced readers to join the interdisciplinary field of evolutionary genomics.
Download or read book The BAM Complex written by Susan Buchanan and published by Humana. This book was released on 2015-10-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is comprised of a collection of experimental protocols for common techniques and strategies used to study the biogenesis of b-barrel outer membrane proteins in Gram-negative bacteria. The BAM Complex: Methods and Protocols guides readers through methods on the function of the BAM complex, the roles played by each of the individual components, the expression and purification of the components, crystallization and structure determination of the components, and how the individual Bam components may assemble into a functional complex. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The BAM Complex: Methods and Protocols will serve as an invaluable reference for those interested in studying the BAM complex.
