Download or read book Isolation and Characterization of Candidate Tumor Suppressor Genes written by Michele Genini and published by . This book was released on 1995 with total page 194 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mapping of the Short Arm of Human Chromosome Three and Isolation and Characterization of a Candidate Tumor Suppressor Gene Protein Tyrosine Phosphatase Receptor Gamma written by Sal Thomas LaForgia and published by . This book was released on 1994 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification and Characterization of Candidate Tumor Suppressor Genes from Chromosomal Region 1p22 in Mantle Cell Lymphomas written by Asha Balakrishnan and published by . This book was released on 2004 with total page 282 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Tumor Suppressor Genes written by Wafik S. El-Deiry and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has become clear that tumors arise from excessive cell proliferation and a c- responding reduction in cell death. Tumors result from the successive accumulation of mutations in key regulatory target genes over time. During the 1980s, a number of oncogenes were characterized, whereas from the 1990s to the present, the emphasis shifted to tumor suppressor genes (TSGs). It has become clear that oncogenes and tumor suppressor genes function in the same pathways, providing positive and ne- tive growth regulatory activities. The signaling pathways controlled by these genes involve virtually every process in cell biology, including nuclear events, cell cycle, cell death, cytoskeletal, cell membrane, angiogenesis, and cell adhesion effects. Tumor suppressor genes are mutated in hereditary cancer syndromes, as well as somatically in nonhereditary cancers. In their normal state, TSGs control cancer development and p- gression, as well as contribute to the sensitivity of cancers to a variety of therapeutics. Understanding the classes of TSGs, the biochemical pathways they function in, and how they are regulated provides an essential lesson in cancer biology. We cannot hope to advance our current knowledge and to develop new and more effective therapies without understanding the relevant pathways and how they influence the present approaches to therapy. Moreover, it is important to be able to access the powerful tools now available to discover these genes, as well as their links to cell biology and growth control.
Download or read book Functional Characterization of Candidate Tumor Suppressor Genes Localized in the Critical Chromosomal Region 13q14 written by Leticia Serra Barrionuevo and published by . This book was released on 2008 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of Two Candidate Tumor Suppressor Genes written by Hau-Yi Paulisally Lo and published by . This book was released on 2017-01-26 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Characterization of Putative Proto Oncogenes and Tumor Suppressor Genes That Are Transcriptionally Deregulated in Breast Cancer written by and published by . This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Candidate proto-oncogenes and tumor suppressor genes were previously isolated in a gene expression study involving breast cancer. The present research focus on the characterization of genetic alterations associated with presenilin-2 (PS-2), a gene identified in the gene expression a%alysis. PS-2 has been implicated in the pathogenesis of Alzheimer's disease, but functional studies indicate that the gene plays a role in pathways commonly perturbed in cancer, hence suggesting that PS-2 may be a target for genetic alterations in tumors. PS-2 maps to a chromosomal region for which LOH/deletion in breast tumors has been reported. We found low PS-2 expression in a fraction of breast tumors. In some cases, the reduction in expression can be attributed to deletion at the PS-2 genomic locus. In addition, we identified two sequence alterations in PS-2 which resulted in amino acid substitutions. Both of the alterations were observed to affect the physiological activity of PS-2, but appear not to be contributing to the Alzheimer's phenotype. The alterations may indeed contribute to mammary tumorigenesis and we are in the process of further exploring this possibility.
Download or read book Functional and Epigenetic Characterization of Silenced Candidate Tumor Suppressor Genes in Cancers written by Ching Gee Choi and published by . This book was released on 2012 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation of Novel Prostate Cancer Tumor Suppressor Genes in African American and Caucasian Men Thru Laser Microdissection and Representation Difference Analysis written by and published by . This book was released on 1999 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Significant progress has been made for all three specific aims. Specific Aim 1: Obtaining metastatic prostate cancer DNA of quality sufficient for Representational Difference Analysis (RDA), has been achieved using alternate means. Specific Aim 2: Employ RDA to identify DNA regions which have been homozygously deleted, has been achieved with identification of a novel region of homozygous deletion on chromosome 12p (Cancer Research, 58, 5652-5655, 1998). Specific Aim 3: To prioritize regions of homozygous deletion based on frequency of deletion of this region in metastatic tumors, and to identify candidate genes within these regions, has been achieved, with previously unidentified deletion of the identified chromosome 12p region in 50% of metastatic prostate cancers studied (Genes, Chromosomes, and Cancer 25:270-276, 1999) . Examination of genes within this region suggests that p27 or TEL to be likely candidates. These results validate this approach and additional studies are underway to identify additional genomic regions of interest in metastatic prostate cancer.
Download or read book Isolation of Novel Prostate Cancer Tumor Suppressor Genes in African American and Caucasian Men Thru Laser Microdissection and Representational Difference Analysis written by and published by . This book was released on 2001 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: We used RDA to identify homozygously deleted DNA clones within metastatic prostate cancer xenograft DNA, and found that all of the homozygously deleted clones mapped to a single region of chromosome 12p. We confirmed that the metastatic lesions from this patient also contained this homozygous deletion, and then analyzed this region in a series of 41 metastatic tumors from 19 patients, where we found that 50% of patients' tumors show loss. This 12p deletion occurs with similar frequency in caucasians and african-americans based on our small sample. We mapped this genomic region, and found that two previously identified potential candidate genes ETV(tel) and CDKN1B(p27) are located there. Analysis of these genes has revealed no sequence changes consistent with an important tumor suppressor role. Extensive analysis of p27 for possible mutational or methylation changes was negative. Major observations from our work include the finding of a homozygous deletion on chromosome 12p in metastatic prostate cancer, considered to be a major indicator that an important gene is nearby on chromosome 12p. Further analysis of this region revealed unexpected high degree of allelic loss in this region. Specific genes in the region were analyzed, and p27 appears to be the likely target, inactivated by loss of one copy. Further analysis was curtailed due to lack of funding.
Download or read book Characterization of Novel Tumor Suppressor Genes DLC 1 and DLC 2 in Hepatocellular Carcinoma written by Chun-Ming Wong and published by Open Dissertation Press. This book was released on 2017-01-27 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Characterization of Novel Tumor Suppressor Genes, DLC-1 and DLC-2, in Hepatocellular Carcinoma" by Chun-ming, Wong, 黃俊銘, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Abstract of thesis entitled Characterization of Novel Tumor Suppressor Genes, DLC-1 and DLC-2, in Hepatocellular Carcinoma Submitted by Wong Chun-Ming For the degree of Doctor of Philosophy at the University of Hong Kong in December 2003 Hepatocellular carcinoma (HCC) is one of the most common malignancies in the world. Although the risk factors are well known, the underlying molecular mechanisms contributing to hepatocarcinogenesis are far from clear. Deletions of chromosomal materials are the most frequent genetic alteration found in HCC. Allelic losses in HCC show a non-random pattern, and chromosomes 8p and 13q are two of the most susceptible chromosomal arms. However, critical regions of loss and putative suppressor genes on 8p and 13q have not been firmly identified. Our experimental data indicated that allelic losses on chromosomes 8p and 13q were frequent in HCC and were associated with aggressive tumor phenotypes. In addition, our results showed recurrent losses on the sub-chromosomal regions 8p21.3-22 and 13q12.3. Such recurrent losses suggest the presence of putative tumor suppressor genes, loss of which may be important to HCC development and progression. In search of candidate tumor suppressor genes in these regions, we identified a novel gene DLC-2 (deleted in liver cancer 2) at 13q12.3. DLC-2 shared a high sequence homology with putative tumor suppressor gene DLC-1 at 8p21.3-22. Both DLC-1 and DLC-2 are GTPase activating proteins that activate the intrinsic GTPase activity of RhoA and Cdc42 and switch them off by converting the active GTP-bound state to the inactive GDP-bound state. Several lines of evidence suggest that aberrant activation of Rho proteins is oncogenic. Thus, it is not surprising that DLC-1 and DLC-2 may function as a tumor suppressor. Although we rarely found somatic mutations in the RhoGAP domain of DLC-1 or DLC-2 mRNA, deletion of DLC-1 or DLC-2 locus as revealed by loss of heterozygosity analysis was frequent in human HCC. In addition, DLC-1 and DLC-2 were significantly underexpressed at mRNA levels in primary HCC, suggesting that loss of DLC-1 and DLC-2 functions as a result of downregulated mRNA expression might contribute to hepatocarcinogenesis. Furthermore, DLC-1 promoter methylation was found in 6 (24%) of 25 primary HCCs and in hepatoma cell lines showing loss of DLC-1 mRNA expression. Our results indicated that both genetic and epigenetic alterations play an important role in inactivation of the DLC-1 gene in hepatocarcinogenesis. We also characterized the tumor suppression function of DLC-1 and DLC-2. We noticed that colony formation was significantly inhibited by transient transfection of DLC-1 cDNA into hepatoma cell lines with no DLC-1 expression. Furthermore, stable expression of DLC-1 mRNA successfully suppressed the proliferation, mitogen- independent growth, and anchorage-independent growth capabilities of SMMC-7721 cells. On the other hand, expression of the GAP domain of DLC-2 in mouse fibroblasts sufficiently repressed Ras signaling and Ras-induced cellular transformation. Overall, our findings suggest that DLC-1 and DLC-2 play an important role in hepatocarcinogenesis. An abstract of 439 words DOI: 10.5353/th_b2776853 Subjects: Liver - Cancer - Genetic aspects Antioncogenes
Download or read book Tumor Suppressor Genes written by Wafik S. El-Deiry and published by Humana PressInc. This book was released on 2003-04-21 with total page 657 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tumor Suppressor Genes provides a timely foundation for understanding cancer. It provides critical information to rapidly gain appreciation of important tumor suppressor genes in human cancer as well as modern methods for their discovery, analysis, and clinical application. It covers all the known tumor suppressor pathways and provides key information on the road to their discovery and use in cancer therapy. Volume I of this text, educates the reader on the relevance of known tumor suppressor genes (TSGs) and the relevance of the biochemical pathways they regulate to human cancer. The reader has an opportunity in volume I to access state-of-the-art protocols that have been successful in the identification of TSGs in the past and which can be applied to isolate novel TSGs. With a novel TSG at hand, the reader has an opportunity in volume II to explore the cell biology and biochemical function of the encoded protein, as well as its physiological role in-vivo. Also contained in volume II, strategies for the use of information on TSGs to develop diagnostic and therapeutic strategies for cancer are presented. This two-volume text brings together the world's most expert researchers in the identification and characterization of tumor suppressor genes.
Download or read book Molecular Genetics of Cancer written by John K. Cowell and published by . This book was released on 1995-07-05 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first volume was published, there has been significant success in isolating genes responsible for particular cancers as well as a major improvement in our understanding of the molecular events leading to tumors. This book explores possible genetic treatments that can suppress cancer cells that have formed tumors and it presents the details of the isolation and characterization of new human cancer genes that have recently been identified. Molecular Genetics of Cancer, 2E is an essential book for anyone involved in cancer research and the search for a cure.
Download or read book Identification of Novel Candidate Tumor Suppressor Genes at 5q and 14q for Multiple Carcinomas by Integrative Genomics and Epigenetics written by Ka Man Ng and published by . This book was released on 2007 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Tumor Suppressor Genes Pathways and isolate strategies written by Wafik S. El-Deiry and published by . This book was released on 2003 with total page 657 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Tumor Suppressor Genes in Human Cancer written by David E. Fisher and published by Springer Science & Business Media. This book was released on 2000-10-26 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: David Fisher, MD, PhD, and an authoritative panel of academic, cutting-edge researchers review and summarize the current state of the field. Describing the broad roles of tumor suppressors from a perspective based in molecular biology and genetics, the authors detail the major suppressors and the pathways they regulate, including cell cycle progression, stress responses, apoptosis, and responses to DNA damage. Leading-edge and forward-looking, Tumor Suppressor Genes in Human Cancer illuminates what is currently known of tumor suppressor genes and their regulation, work that is already beginning to revolutionize cancer target elucidation, drug discovery, and treatment design.
Download or read book Journal of the National Cancer Institute written by and published by . This book was released on 2012 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt:
