Download or read book Chromatography in Biotechnology written by Csaba Horváth and published by . This book was released on 1993 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: The most up-to-date compilation of significant research on preparative liquid chromatography used for the separation of biomolecules and proteins. Presents recent advances in high-performance liquid chromatographic techniques for the isolation and purification of bioproducts in the laboratory and manufacturing plant. Discusses novel approaches to the preparative/process chromatography of complex carbohydrate and glycoconjugates. Also describes recent advances in column materials.
Download or read book High Performance Liquid Chromatography in Biotechnology written by William S. Hancock and published by Wiley-Interscience. This book was released on 1990-10-24 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: High Performance Liquid Chromatography in Biotechnology Edited by William S. Hancock This important book details the broad range of analytical techniques that have made HPLC so successful in the separation and quantitation of product variants-a procedure essential to quality assurance of protein pharmaceuticals. Outlined are the major chromatographic techniques-reversed phase, ion exchange, affinity and hydrophobic interaction chromatography-as well as their role in specific separations. Also described are the methods required for a quality control program. Reflecting the heightened concern for product integrity since the advent of new protein pharmaceuticals by recombinant-DNA techniques, this critical reference will enhance the libraries of analytical chemists, biochemists, and chemical engineers working with HPLC. 1990 (0-471-82584-0) 624 pp.
Download or read book High Performance Liquid Chromatography written by Elena Katz and published by John Wiley & Sons. This book was released on 1996-04-11 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: This study of high performance liquid chromatography (HPLC) aims to provide bioresearchers with a sound understanding of the principles, advantages and limitations of the technique. It combines discussion of theory with applications of HPLC to biotechnology.
Download or read book Chromatographic and Membrane Processes in Biotechnology written by C.A. Costa and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 465 pages. Available in PDF, EPUB and Kindle. Book excerpt: Separation processes in biotechnology are of increasing industrial importance since they entail the major costs of bioprocessing especially when high purity is required. Chromatography and membranes are two of the most important technologies used for direct treatment of fermentation broths as well as for high resolution steps in product purification. The theoretical foundations of chromatographic and membrane processes are well understood for the case of small molecules. Nevertheless there is a need to adapt and further develop that knowledge to the processing of large biological molecules. This is being achieved with the contribution of other areas like molecular biology and materials science. The objective of this NATO Advanced Study Institute is to present an updated treatment of the fundamentals of chromatographic and membrane processes with special relevance in bioprocessing.This volume collects the lectures presented at this Institute. The lectures are arranged in five chapters. Chapter I deals with chromatographic processes covering topics like equilibrium, kinetics and contacting devices. Membrane processes and some applications in biotechnology are treated in chapter 2. Chapter 3 is devoted to affinity chromatographic and membrane processes. Chapter 4 considers the current developments on chromatographic supports and membranes both from the constitutive materials and form points of view. Scale-up, optimization and reaction/separation integration are the topics covered in chapter 5. We are very grateful to all lecturers and participants that made possible this Institute. Financial support from NATO Scientific Affairs Division, INIC, JNICT, FLAD, University of Ac;ores and DRT Ac;ores is gratefully acknowledged.
Download or read book Protein Chromatography written by Giorgio Carta and published by John Wiley & Sons. This book was released on 2020-06-02 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: An all-in-one practical guide on how to efficiently use chromatographic separation methods Based on a training course that teaches the theoretical as well as practical aspects of protein bioseparation to bioprocess professionals, this fully updated and revised new edition offers comprehensive coverage of continuous chromatography and provides readers with many relevant examples from the biopharmaceutical industry. Divided into two large parts, Protein Chromatography: Process Development and Scale-Up, Second Edition presents all the necessary knowledge for effective process development in chromatographic bioseparation, both on small and large scale. The first part introduces chromatographic theory, including process design principles, to enable the reader to rationalize the set-up of a bioseparation process. The second part illustrates by way of case studies and sample protocols how the theory learned in the first part may be applied to real-life problems. Chapters look at: Downstream Processing of Biotechnology Products; Chromatography Media; Laboratory and Process Columns and Equipment; Adsorption Equilibrium; Rate Processes; and Dynamics of Chromatography Columns. The book closes with chapters on: Effects of Dispersion and Rate Processes on Column Performance; Gradient Elution Chromatography; and Chromatographic Column Design and Optimization. -Presents the most pertinent examples from the biopharmaceutical industry, including monoclonal antibodies -Provides an overview of the field along with design tools and examples illustrating the advantages of continuous processing in biopharmaceutical productions -Focuses on process development and large-scale bioseparation tasks, making it an ideal guide for the professional bioengineer in the biotech and pharma industries -Offers field-tested information based on decades of training courses for biotech and chemical engineers in Europe and the U.S. Protein Chromatography: Process Development and Scale-Up, Second Edition will appeal to biotechnologists, analytical chemists, chromatographers, chemical engineers, pharmaceutical industry, biotechnological industry, and biochemists.
Download or read book High Performance Liquid Chromatography Principles And Methods In Biotechnology written by Ed: Elena D. Katz and published by John Wiley & Sons. This book was released on 1996 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: This study of high performance liquid chromatography (HPLC) aims to provide bioresearchers with a sound understanding of the principles, advantages and limitations of the technique. It combines discussion of theory with applications of HPLC to biotechnology.
Download or read book Applications of Ion Chromatography for Pharmaceutical and Biological Products written by Lokesh Bhattacharyya and published by John Wiley & Sons. This book was released on 2012-02-10 with total page 421 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a comprehensive source of information on the application of ion chromatography (IC) in the analysis of pharmaceutical drugs and biologicals. This book, with contributors from academia, pharma, the biotech industry, and instrument manufacturing, presents the different perspectives, experience, and expertise of the thought leaders of IC in a comprehensive manner. It explores potential IC applications in different aspects of product development and quality control testing. In addition, an appendix section gives information on critical physical and chromatographic parameters related to IC and information on current manufacturers of IC systems, columns, and other components.
Download or read book Preparative Chromatography for Separation of Proteins written by Arne Staby and published by John Wiley & Sons. This book was released on 2017-02-02 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt: Preparative Chromatography for Separation of Proteins addresses a wide range of modeling, techniques, strategies, and case studies of industrial separation of proteins and peptides. • Covers broad aspects of preparative chromatography with a unique combination of academic and industrial perspectives • Presents Combines modeling with compliantce useing of Quality-by-Design (QbD) approaches including modeling • Features a variety of chromatographic case studies not readily accessible to the general public • Represents an essential reference resource for academic, industrial, and pharmaceutical researchers
Download or read book Modern Advances in Chromatography written by Ruth Freitag and published by Springer. This book was released on 2003-07-01 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromatography is the separation method for biological molecules from the analytical to the preparative scale. Few of the recent advances in life science, whether in "proteomics" or in the industrial production of recombinant therapeutics, would have been possible without the help of chromatographic separations. Concomitantly, chromatography nowadays stands for much more than a batch column packed with porous particles. In this book eminent experts from academia and industry introduce the reader to some of the recent new developments in this exciting area. Ranging from evolving nanoanalytical techniques to advances in the material sciences and artificial antibodies and finally continuous large scale separations of the most fragile biologicals, this book should provide interesting reading material for students and practitioners from various fields.
Download or read book Separation And Purification Technique In Biotechnology written by Frederick J. Dechow and published by . This book was released on 2005-01-01 with total page 527 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction* Adsorption * Ion Exchange * Column Chromatography Processes * Affinity Chromatography * Index.
Download or read book Affinity Chromatography written by Michael Zachariou and published by Springer Science & Business Media. This book was released on 2008 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, Affinity Chromatography: Methods and Protocols, Second Edition, provides the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. This second edition expands on the first edition by introducing more state-of-the-art protocols used in affinity chromatography. This new edition also describes protocols that demonstrate the concept of affinity chromatography being applied to meet the modern high throughput screening demands of researchers and development scientists whilst expanding on some more traditional affinity chromatography approaches that have become of greater interest to separation scientists. Chapters in this cutting-edge text expand on affinity chromatography techniques that currently enjoy frequent citation in the literature from those purifying biomolecules. Other chapters include protocols describing the use of a variety of fusion tags as well as how to cleave them, so as to allow the scientists to study the native phenotype of the protein. Renowned researchers also include protocols detailing diverse applications of affinity chromatography such as its use in catalytic reactions, DNA purification, whole cell separations and for the isolation of phosphorylated proteins. Affinity Chromatography: Methods and Protocols, Second Edition, is an essential reference for those interested in separation sciences, particularly in the pharmaceutical and biological research sectors, that have an interest in isolating macromolecules rapidly, quantitatively, and with high purity.
Download or read book Affinity Chromatography written by J. Turková and published by Elsevier. This book was released on 1978-01-01 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioaffinity chromatography has increasingly become the method of choice for the purification, determination or removal of many biologically active substances.
Download or read book Protein Chromatography written by Dermot Walls and published by Humana Press. This book was released on 2011-04-06 with total page 527 pages. Available in PDF, EPUB and Kindle. Book excerpt: A prerequisite for elucidating the structure and function of any protein is the prior purification of that protein. This necessity has led to the development of many purification schemes and chromatographic methods for the isolation of native proteins from complex sources. In Protein Chromatography: Methods and Protocols, leading researchers present clear protocol-style chapters that are suitable for newcomers and experts alike. The book opens with vital topics in protein biochemistry, addressing such areas as protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the contrasting challenges that microfluidics and scale-up production pose to the investigator, and then it segues into key methods involving the generation and purification of recombinant proteins through recombinant antibody production and the tagging of proteins, amongst other means, as well as many variations on classic techniques such as ion-exchange and immunoaffinity chromatography. Written in the highly successful Methods in Molecular BiologyTM series format, protocols chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Protein Chromatography: Methods and Protocols will greatly aid scientists in establishing these essential techniques in their own laboratories and furthering our understanding of the many imperative functions of proteins.
Download or read book Protein Chromatography written by Giorgio Carta and published by John Wiley & Sons. This book was released on 2010-06-08 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: With its focus on process development and large-scale bioseparation tasks, this is tailor-made reading for the professional bioengineer in both the biotech and pharmaceutical industries. Following a tried-and-tested concept, this guide has been developed over several years in training courses for biotech and chemical engineers in Europe and the U.S. The first part deals with the theory, introducing chromatography and its dynamics, as well as discussing mass transfer and dispersion effects. The second part then goes on to cover equipment and protocols, determining the retention factor and HETP from isocratic and elution experiments, as well as the mass transfer and intraparticle diffusivity from batch and shallow-bed adsorption experiments.
Download or read book Process Chromatography written by G. K. Sofer and published by . This book was released on 1989 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromatography has today become an integral part of the biotechnology industry, playing a vital role in the purification of biologicals from natural sources as those produced by recombinant DNA and hybridoma techniques. This book is intended as a practical guide and reference source for all those involved in the development of economic chromatographic purification processes. Its purpose extends beyond the description of individual methods to teach the integration of steps, both up and downstream, that lead to a complete process for the successful purification of a desired protein. Researchers, advanced students, senior technicians, and process plant managers working in the biotechnology, biochemical, and pharmaceutical industries will find this text indispensible.
Download or read book Affinity Chromatography and Biological Recognition written by Irwin Chaiken and published by Elsevier. This book was released on 2012-12-02 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity Chromatography and Biological Recognition contains manuscripts presented at the Fifth International Symposium on Affinity Chromatography and Biological Recognition convened in June 12-17, 1983, at St. John's College in Annapolis, Maryland. Organized into six parts encompassing 82 chapters, the book begins by examining the growing synergism between affinity methods and the understanding and study of basic principles of biological recognition. The book then focuses on the trends and progress in the design and application of affinity methods for isolation, therapeutics, diagnostics, and biotechnology. Significant chapters are devoted to the contributions of affinity methodology in such areas as cell membrane receptors, quantitative properties of macromolecular interactions, microscale analytical and preparative applications of high performance affinity chromatography, antibodies as in vivo and in vitro diagnostic and therapeutic agents, and drug targeting. This volume will be a stimulus for broad and creative application of affinity concepts and methods in many fields of biomedical research and biotechnology.
Download or read book Chromatography in Biotechnology written by and published by . This book was released on 1993 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
