Download or read book The Yeast Two hybrid System written by Paul L. Bartel and published by Oxford University Press, USA. This book was released on 1997 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume, part of the Advances in Molecular Biology series, presents work by pioneers in the field and is the first publication devoted solely to the yeast two-hybrid system. It includes detailed protocols, practical advice on troubleshooting, and suggestions for future development. In addition, it illustrates how to construct an activation domain hybrid library, how to identify mutations that disrupt an interaction, and how to use the system in mammalian cells. Many of the contributors have developed new applications and variations of the technique.
Download or read book Two Hybrid Systems written by Paul N. MacDonald and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: The yeast two-hybrid system is one of the most widely used and productive techniques available for investigating the macromolecular interactions that affect virtually all biological processes. In Two-Hybrid Systems: Methods and Protocols, Paul N. MacDonald has assembled a collection of these powerful molecular tools for examining and characterizing protein-protein, protein-DNA, and protein-RNA interactions. The techniques range from the most basic (introducing plasmids into yeasts, interaction assays, and recovering the plasmids from yeast) to the most advanced alternative strategies (involving one-hybrid, split two-hybrid, three-hybrid, membrane recruitment systems, and mammalian systems). Methods are also provided for dealing with the well-known problems of artifacts and false positives and for identifying the interacting partners in important biological systems, including the Smad and nuclear receptor pathways. To ensure ready reproducibility and robust results, each technique is described in step-by-step detail by researchers who employ it regularly. Comprehensive and highly practical, Two-Hybrid Systems: Methods and Protocols not only reveals how the great variety of plasmid vectors and approaches may be optimally deployed, but also quickly empowers novices to establish two-hybrid systems in their laboratories, and experienced researchers to expand their repertoire of techniques.
Download or read book An Introduction to Hybrid Dynamical Systems written by Arjan J. van der Schaft and published by Springer. This book was released on 2007-10-03 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is about dynamical systems that are "hybrid" in the sense that they contain both continuous and discrete state variables. Recently there has been increased research interest in the study of the interaction between discrete and continuous dynamics. The present volume provides a first attempt in book form to bring together concepts and methods dealing with hybrid systems from various areas, and to look at these from a unified perspective. The authors have chosen a mode of exposition that is largely based on illustrative examples rather than on the abstract theorem-proof format because the systematic study of hybrid systems is still in its infancy. The examples are taken from many different application areas, ranging from power converters to communication protocols and from chaos to mathematical finance. Subjects covered include the following: definition of hybrid systems; description formats; existence and uniqueness of solutions; special subclasses (variable-structure systems, complementarity systems); reachability and verification; stability and stabilizability; control design methods. The book will be of interest to scientists from a wide range of disciplines including: computer science, control theory, dynamical system theory, systems modeling and simulation, and operations research.
Download or read book Impulsive and Hybrid Dynamical Systems written by Wassim M. Haddad and published by Princeton University Press. This book was released on 2014-09-08 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book develops a general analysis and synthesis framework for impulsive and hybrid dynamical systems. Such a framework is imperative for modern complex engineering systems that involve interacting continuous-time and discrete-time dynamics with multiple modes of operation that place stringent demands on controller design and require implementation of increasing complexity--whether advanced high-performance tactical fighter aircraft and space vehicles, variable-cycle gas turbine engines, or air and ground transportation systems. Impulsive and Hybrid Dynamical Systems goes beyond similar treatments by developing invariant set stability theorems, partial stability, Lagrange stability, boundedness, ultimate boundedness, dissipativity theory, vector dissipativity theory, energy-based hybrid control, optimal control, disturbance rejection control, and robust control for nonlinear impulsive and hybrid dynamical systems. A major contribution to mathematical system theory and control system theory, this book is written from a system-theoretic point of view with the highest standards of exposition and rigor. It is intended for graduate students, researchers, and practitioners of engineering and applied mathematics as well as computer scientists, physicists, and other scientists who seek a fundamental understanding of the rich dynamical behavior of impulsive and hybrid dynamical systems.
Download or read book Bacterial Protein Secretion Systems written by Laure Journet and published by Humana Press. This book was released on 2017-07-01 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details protocols that cover the broad arsenal of techniques used to study a secretion system from A to Z. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Protein Secretion Systems: Methods and Protocol aims to provide techniques that are not restricted to the study of secretion systems but are also of specific interest for any researcher interested on multi-protein complexes of the bacterial cell envelope.
Download or read book Hybrid Feedback Control written by Ricardo G. Sanfelice and published by Princeton University Press. This book was released on 2021-01-12 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Hybrid systems are those that-unlike classical systems-exhibit both discrete changes, or "jumps", and continuous changes, or "flow." The canonical example of a hybrid system is a bouncing ball: the ball's speed changes continuously between bounces, but there is a discrete jump in velocity each time the ball impacts the ground. Hybrid systems feature widely across disciplines, including in biology, computer science, and mechanical engineering; examples range from fireflies to self-driving cars. Although classical control theory provides powerful tools for analyzing systems that exhibit either flow or jumps, it is ill-equipped to handle hybrid systems, which feature both behaviors. In Hybrid Feedback Control, Ricardo Sanfelice presents a self-contained introduction to the control of hybrid systems, and develops new tools for their design and analysis. This monograph uses hybrid systems notation to present a new, unified control theory framework, thus filling an important gap in the control theory literature. In addition to presenting this theoretical framework, the book also includes a variety of examples and exercises, a Matlab toolbox, and a summary at the beginning of each chapter. The book was originally used in a series of lectures on the topic, and will find a modest amount of crossover course use. The book will also find use outside the field of control, particularly in dynamical systems theory, applied mathematics, and computer science"--
Download or read book Protein Protein Interactions written by Weibo Cai and published by BoD – Books on Demand. This book was released on 2012-03-30 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are indispensable players in virtually all biological events. The functions of proteins are coordinated through intricate regulatory networks of transient protein-protein interactions (PPIs). To predict and/or study PPIs, a wide variety of techniques have been developed over the last several decades. Many in vitro and in vivo assays have been implemented to explore the mechanism of these ubiquitous interactions. However, despite significant advances in these experimental approaches, many limitations exist such as false-positives/false-negatives, difficulty in obtaining crystal structures of proteins, challenges in the detection of transient PPI, among others. To overcome these limitations, many computational approaches have been developed which are becoming increasingly widely used to facilitate the investigation of PPIs. This book has gathered an ensemble of experts in the field, in 22 chapters, which have been broadly categorized into Computational Approaches, Experimental Approaches, and Others.
Download or read book Hybrid Technologies for Power Generation written by Massimiliano Lo Faro and published by Academic Press. This book was released on 2021-10-30 with total page 530 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hybrid Technologies for Power Generation addresses the topics related to hybrid technologies by coupling conventional thermal engines with novel technologies, including fuel cells, batteries, thermal storage and electrolysis, and reporting on the most recent advances concerning transport and stationary applications. Potential operating schemes of hybrid power generation systems are covered, highlighting possible combinations of technology and guideline selection according to the energy demands of end-users. Going beyond state-of-the-art technological developments for processes, devices and systems, this book discusses the environmental impact and existing hurdles of moving from a single device to new approaches for efficient energy generation, transfer, conversion, high-density storage and consumption. By describing the practical viability of novel devices coupled to conventional thermal devices, this book has a decisive impact in energy system research, supporting those in the energy research and engineering communities. - Covers detailed thermodynamic requirements for multiple smart technologies included in hybrid systems (i.e., FC, electrolysers, supercapacitors, batteries, thermal storage, etc.) - Features fundamental analysis and modeling to optimize the combination of smart technologies with traditional engines - Details protocols for the analysis, operation and requirements of large-scale production
Download or read book Circadian Rhythms written by Ezio Rosato and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 557 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is an in-depth examination of circadian biology, presented by leading researchers in the field. Methods for analysis of rhythmic readouts in select model organisms are included. This cutting-edge collection of protocols is adaptable for research at every level, and represents the huge strides that chronobiologists have made over the past two decades. Circadian biologists at all research levels will realize tremendous benefit from this extraordinary collection.
Download or read book Protein Interaction Networks written by Aidong Zhang and published by Cambridge University Press. This book was released on 2009-04-06 with total page 283 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis of protein-protein interactions is fundamental to the understanding of cellular organization, processes, and functions. Proteins seldom act as single isolated species; rather, proteins involved in the same cellular processes often interact with each other. Functions of uncharacterized proteins can be predicted through comparison with the interactions of similar known proteins. Recent large-scale investigations of protein-protein interactions using such techniques as two-hybrid systems, mass spectrometry, and protein microarrays have enriched the available protein interaction data and facilitated the construction of integrated protein-protein interaction networks. The resulting large volume of protein-protein interaction data has posed a challenge to experimental investigation. This book provides a comprehensive understanding of the computational methods available for the analysis of protein-protein interaction networks. It offers an in-depth survey of a range of approaches, including statistical, topological, data-mining, and ontology-based methods. The author discusses the fundamental principles underlying each of these approaches and their respective benefits and drawbacks, and she offers suggestions for future research.
Download or read book Dengue Virus written by Ronaldo Mohana-Borges and published by Humana. This book was released on 2021-10-29 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores various aspects of the dengue virus and its four serotypes (DENV1-4). Beginning with a section on protocols to produce DENV and its proteins that are fundamental for many biophysical, biochemical, immunological, and cellular studies, the book continues by covering protocols to study the interactions between DENV and cellular proteins, DENV immunopathogenesis and diagnosis, as well as recent advances in animal models that can be used in studies of DENV immunopathogenesis and vaccine development. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Dengue Virus: Methods and Protocols serves as an ideal guide for researchers attempting to further understand this critical threat to worldwide public health.
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protein Microarray Technology written by Dev Kambhampati and published by Wiley-Blackwell. This book was released on 2004-03 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein microarrays soon will become one of the major tools for realizing the goals of drug discovery research in the post-genome era. The book is aimed at life science researchers and scientists working in drug and pharma industry.
Download or read book Recombinant Protein Protocols written by Rocky S. Tuan and published by Humana. This book was released on 2013-08-23 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: A major success story of modem molecular biology is the development of technologies to clone and express specific genes. Current applications of recombinant gene products cover a wide spectrum, including gene therapy, production of bioactive pharmaceuticals, synthesis of novel biopolymers, agriculture and animal husbandry, and so on. Inherent in bringing these appli cations to fruition is the need to design "expression constructs" that will per mit the ready and specific detection and isolation of the defined recombinant gene products. Recombinant Protein Protocols grows out of the need for a laboratory manual on the detection and isolation of recombinantly expressed genes that covers both the background information and the practical laboratory recipes for these analyses. In this book, detailed and contemporary protocols are col lected to provide the reader with a wide-ranging number of methodologies to enhance the detection and isolation of their gene product(s) of interest. A large number of molecular tags and labels and their usage are described, including enzymes, ligand-binding moieties, immunodetectable molecules, as well as methods to detect interactive proteins, and gene expression-mediated alter ations in cellular activity. Chapters on in situ detection of gene expression deal with technologies that are currently being applied to the study of gene function and activity. Highlights of applications for recombinant gene expres sion technologies are provided to give readers exciting perspectives on the future of such technologies.
Download or read book Qualitative Theory of Hybrid Dynamical Systems written by Alexey S. Matveev and published by Springer Science & Business Media. This book was released on 2000-03-23 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: The emerging area of hybrid dynamical systems lies at the interface of control theory and computer science, i.e., analogue 'and' digital aspects of systems. This new monograph presents state-of-the-art concepts, methods and tools for analyzing and describing hybrid dynamical systems.
Download or read book Hybrid Dynamical Systems written by Hai Lin and published by Springer Nature. This book was released on 2021-11-18 with total page 455 pages. Available in PDF, EPUB and Kindle. Book excerpt: A graduate-level textbook, Hybrid Dynamical Systems provides an accessible and comprehensive introduction to the theory of hybrid systems. It emphasizes results that are central to a good understanding of the importance and role of such systems. The authors have developed the materials in this book while teaching courses on hybrid systems, cyber-physical systems, and formal methods. This textbook helps students to become familiar with both the major approaches coloring the study of hybrid dynamical systems. The computer science and control systems points of view – emphasizing discrete dynamics and real time, and continuous dynamics with switching, respectively – are each covered in detail. The book shows how the behavior of a system with tightly coupled cyber- (discrete) and physical (continuous) elements can best be understood by a model simultaneously encompassing all the dynamics and their interconnections. The theory presented is of fundamental importance in a wide range of emerging fields from next-generation transportation systems to smart manufacturing.Features of the text include: extensive use of examples to illustrate the main concepts and to provide insights additional to those acquired from the main text; chapter summaries enabling students to assess their progress; end-of-chapter exercises, which test learning as a course proceeds; an instructor’s guide showing how different parts of the book can be exploited for different course requirements; and a solutions manual, freely available for download by instructors adopting the book for their teaching. Access to MATLAB and Stateflow is not required but would be beneficial, especially for exercises in which simulations are a key tool.
Download or read book Hybrid Energy System Models written by Asmae Berrada and published by Academic Press. This book was released on 2020-12-10 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hybrid Energy System Models presents a number of techniques to model a large variety of hybrid energy systems in all aspects of sizing, design, operation, economic dispatch, optimization and control. The book's authors present a number of new methods to model hybrid energy systems and several renewable energy systems, including photovoltaic, solar plus wind and hydropower, energy storage, and combined heat and power systems. With critical modeling examples, global case studies and techno-economic modeling integrated in every chapter, this book is essential to understanding the development of affordable energy systems globally, particularly from renewable resources. With a detailed overview and a comparison of hybrid energy systems used in different regions, as well as innovative hybrid energy system designs covered, this book is useful for practicing power and energy engineers needing answers for what factors to consider when modeling a hybrid energy system and what tools are available to model hybrid systems. Combines research on several renewable energy systems, energy storage, and combined heat and power systems into a single informative resource on hybrid energy systems Includes significant global case studies of current and novel modeling techniques for comparison Covers numerical simulations of hybrid systems energy modeling and applications
