Download or read book Transgenic Switchgrass Panicum Virgatum L Targeted for Reduced Recalcitrance to Bioconversion written by and published by . This book was released on 2016 with total page 10 pages. Available in PDF, EPUB and Kindle. Book excerpt: Five different types of transgenic (GAUT4, miRNA, MYB4, COMT and FPGS) Panicum virgatum L. (switchgrass) were grown in a field in Knoxville, Tenn., USA over two consecutive years between 2011 and 2015 in separate experiments. Clonal replicates were established (year-one) and produced much greater biomass during the second year. After each growing season the above ground biomass was analyzed for cell wall sugars and for recalcitrance to enzymatic digestibility, and biofuel using a separate hydrolysis and fermentation (SHF) screen. Here, each transgenic event and control had more glucan, xylan and less ethanol (g/g basis) from the second year of growth relative to the first year plants. There was no correlation between plant carbohydrate content and biofuel production. In each of cell wall-targeted transgenics, GAUT4, MYB4, COMT and FPGS, the second year of growth resulted in increased carbohydrate abundance (up to 12%) and reduced recalcitrance through higher ethanol yields (up to 21%) over the non-transgenic control plants.
Download or read book Reproduction and Bioconfinement of MiR156 Transgenic Switchgrass Panicum Virgatum L written by Chelsea Renai Johnson and published by . This book was released on 2017 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic engineering of switchgrass (Panicum virgatum L.), an emerging cellulosic bioenergy feedstock, has been performed to alter cell walls for improved biofuel conversion. However, gene flow from transgenic switchgrass presents regulatory issues that may prevent commercialization of the genetically engineered crop in the eastern United States. Depending on its expression level, microRNA156 (miR156) can reduce, delay or eliminate flowering, which may be useful to mitigate transgene flow. However, flowering transition is dependent upon both environmental and genetic cues. In this study of transgenic switchgrass, two low (T14 and T35) and two medium (T27 and T37) miR156 overexpressing 'Alamo' lines and nontransgenic control plants were used. A two-year field experiment was performed to compare flowering, reproduction, and biomass yield in eastern Tennessee, U.S.A. Growth chamber studies assessed temperature and photoperiod effects on flowering and reproduction across a simulated latitudinal cline. In the field, medium miR156 overexpression line T37 resulted in the best overall combination of bioconfinement and biomass production. Though line T37 did flower, not all plants produced panicles, and panicle production was delayed in both years. Line T37 also produced fewer panicles, with a 65.9% reduction in year one and 23.8% reduction in year two over controls. T37 panicles produced 70.6% less flowers than control panicles during the second field year with commensurate decreased seed yield: 1205 seeds per plant vs. 18,539 produced by each control. These results are notable given that line T37 produced equivalent vegetative aboveground biomass as controls. In latitudinal simulation growth chambers, elevated temperatures and decreased daylength promoted flowering of the miR156 transgenic switchgrass lines. As temperatures increased and day lengths decreased, more plants in lines T35, T37, and controls produced panicles. The simulated (Ecuador) tropical conditions were the only chambers in which three of the four transgenic lines flowered. These results suggest that miR156 overexpression levels found in transgenic line T37 can be useful for bioconfinement, and the plants can significantly reproduce in tropical conditions, which would enable plant breeding for line improvement. Furthermore, the study suggests additional ways that miR156 can be manipulated to improve both biomass production and bioconfinement.
Download or read book Field Evaluation of Transgenic Switchgrass Plants Overexpressing PvMYB4 for Reduced Biomass Recalcitrance written by and published by . This book was released on 2015 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt: High biomass yields and minimal agronomic input requirements have made switchgrass, Panicum virgatum L., a leading candidate lignocellulosic bioenergy crop. Large-scale lignocellulosic biofuel production from such crops is limited by the difficulty to deconstruct cell walls into fermentable sugars: the recalcitrance problem. In this study, we assessed the field performance of switchgrass plants overexpressing the switchgrass MYB4 (PvMYB4) transcription factor gene. PvMYB4 transgenic switchgrass can have great lignin reduction, which commensurately increases sugar release and biofuel production. Our results over two growing seasons showed that one transgenic event (out of eight) had important gains in both biofuel (32% more) and biomass (63% more) at the end of the second growing season relative to non-transgenic controls. These gains represent a doubling of biofuel production per hectare, which is the highest gain reported from any field-grown modified feedstock. In contrast to this transgenic event, which had relatively low ectopic overexpression of the transgene, five of the eight transgenic events planted did not survive the first field winter. The dead plants were all high-overexpressing events that performed well in the earlier greenhouse studies. Disease susceptibility was not compromised in any transgenic events over the field experiments. These results demonstrate the power of modifying the expression of an endogenous transcription factor to improve biofuel and biomass simultaneously, and also highlight the importance of field studies for "sorting" transgenic events. In conclusion, further research is needed to develop strategies for fine-tuning temporal-spatial transgene expression in feedstocks to optimize desired phenotypes.
Download or read book Genetic Modification of Switchgrass Panicum Virgatum L for Improvement of Plant Architecture Biomass Productivity and Sugar Release Efficiency for Biofuel written by Wegi Aberra Wuddineh and published by . This book was released on 2015 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: Switchgrass (Panicum virgatum L.) is a leading candidate bioenergy crop for sustainable biofuel production. To ensure its economic viability, tremendous improvements in switchgrass biomass productivity and recalcitrance to enzymatic saccharification are needed. Genetic manipulation of lignin biosynthesis by targeting transcriptional regulators of higher level domains of lignin biosynthesis and other complex traits could alter several bioenergy-desirable traits at once. A three-pronged approach was made in the dissertation research to target one plant growth regulator and transcription factors to alter plant architecture a nd cell wall biosynthesis. Gibberellin (GA) catabolic enzymes, GA 2-oxidases (GA2oxs), were utilized to alternatively modify the lignin biosynthesis pathway as GA is known to play a role in plant lignification. Constitutive overexpression of switchgrass C20 [C20] GA2ox genes altered plant morphology and modified plant architecture by increasing the number of tillers. Moreover, transgenic plants exhibited reduced lignin especially in leaves accompanied by 15% increase in sugar release (glucose). The Knotted1 (PvKN1) TF, a putative repressor of lignin biosynthesis genes, was identified and evaluated for improving biomass characteristics of switchgrass for biofuel. Its ectopic overexpression in switchgrass altered the expression of genes in the lignin, cellulose and hemicellulose biosynthesis, and GA signalling pathways. Consequently, transgenic lines displayed altered growth phenotypes particularly at early stages of vegetative development and moderate changes in lignin content accompanied by improved sugar release by up to 16%. The APETALA2/ ethylene responsive factor (AP2/ERF) TFs are key putative targets for engineering plants not only so they can withstand adverse environmental factors but also confer modified cell wall characteristics. To facilitate this, a total of 207 switchgrass AP2/ERF TFs comprising 3 families (AP2, ERF and related to API3/VP (RAV)) were identified. Sequence analysis for conserved putative motifs and expression pattern analysis delimited key genes for manipulation of switchgrass. To that end, the PvERF001 TF gene was ectopically overexpressed resulting in improved biomass yield and sugar release efficiency. The transgenic plants and knowledge produced in this research will be used to create new lines of switchgrass with combined novel traits to address needs in biofuel production and sustainable plant cultivation to enable the development of the bioeconomy.
Download or read book Identification and Overexpression of a Knotted1 like Transcription Factor in Switchgrass Panicum Virgatum L for Lignocellulosic Feedstock Improvement written by and published by . This book was released on 2016 with total page 15 pages. Available in PDF, EPUB and Kindle. Book excerpt: High biomass production and wide adaptation has made switchgrass (Panicum virgatum L.) an important candidate lignocellulosic bioenergy crop. One major limitation of this and other lignocellulosic feedstocks is the recalcitrance of complex carbohydrates to hydrolysis for conversion to biofuels. Lignin is the major contributor to recalcitrance as it limits the accessibility of cell wall carbohydrates to enzymatic breakdown into fermentable sugars. Therefore, genetic manipulation of the lignin biosynthesis pathway is one strategy to reduce recalcitrance. Here, we identified a switchgrass Knotted1 transcription factor, PvKN1, with the aim of genetically engineering switchgrass for reduced biomass recalcitrance for biofuel production. Gene expression of the endogenous PvKN1 gene was observed to be highest in young inflorescences and stems. Ectopic overexpression of PvKN1 in switchgrass altered growth, especially in early developmental stages. Transgenic lines had reduced expression of most lignin biosynthetic genes accompanied by a reduction in lignin content suggesting the involvement of PvKN1 in the broad regulation of the lignin biosynthesis pathway. Moreover, the reduced expression of the Gibberellin 20-oxidase (GA20ox) gene in tandem with the increased expression of Gibberellin 2-oxidase (GA2ox) genes in transgenic PvKN1 lines suggest that PvKN1 may exert regulatory effects via modulation of GA signaling. Furthermore, overexpression of PvKN1 altered the expression of cellulose and hemicellulose biosynthetic genes and increased sugar release efficiency in transgenic lines. Our findings demonstrated that switchgrass PvKN1 is a putative ortholog of maize KN1 that is linked to plant lignification and cell wall and development traits as a major regulatory gene. Therefore, targeted overexpression of PvKN1 in bioenergy feedstocks may provide one feasible strategy for reducing biomass recalcitrance and simultaneously improving plant growth characteristics.
Download or read book Compendium of Bioenergy Plants written by Hong Luo and published by CRC Press. This book was released on 2014-03-14 with total page 463 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains the most comprehensive reviews on the latest development of switchgrass research including the agronomy of the plant, the use of endophytes and mycorrhizae for biomass production, genetics and breeding of bioenergy related traits, molecular genetics and molecular breeding, genomics, transgenics, processing, bioconversion, biosyst
Download or read book Hybridization of Downregulated COMT Transgenic Switchgrass Lines with Field selected Switchgrass for Improved Biomass Traits written by and published by . This book was released on 2016 with total page 15 pages. Available in PDF, EPUB and Kindle. Book excerpt: Transgenic switchgrass (Panicum virgatum L.) has been produced for improved cell walls for biofuels. For instance, downregulated caffeic acid 3-O-methyltransferase (COMT) switchgrass produced significantly more biomass and biofuel than the non-transgenic progenitor line. In this present study we sought to further improve biomass characteristics by crossing the downregulated COMT T1 lines with high-yielding switchgrass accessions in two genetic backgrounds ('Alamo' and 'Kanlow'). Crosses and T2 progeny analyses were made under greenhouse conditions to assess maternal effects, plant morphology and yield, and cell wall traits. Female parent type influenced morphology, but had no effect on cell wall traits. T2 hybrids produced with T1 COMT-downregulated switchgrass as the female parent were taller, produced more tillers, and produced 63% more biomass compared with those produced using the field selected accession as the female parent. Transgene status (presence or absence of transgene) influenced both growth and cell wall traits. T2 transgenic hybrids were 7% shorter 80 days after sowing and produced 43% less biomass than non-transgenic null-segregant hybrids. Cell wall-related differences included lower lignin content, reduced syringyl-to-guaiacyl (S/G) lignin monomer ratio, and a 12% increase in total sugar release in the T2 transgenic hybrids compared to non-transgenic null segregants. This is the first study to evaluate the feasibility of transferring the low-recalcitrance traits associated with a transgenic switchgrass line into high-yielding field varieties in an attempt to improve growth-related traits. Lastly, our results provide insights into the possible improvement of switchgrass productivity via biotechnology paired with plant breeding.
Download or read book Evaluation of Hall s Panicgrass Panicum Hallii Vasey as a Model System for Genetic Modification of Recalcitrance in Switchgrass Panicum Virgatum L written by Joshua Nathaniel Grant and published by . This book was released on 2017 with total page 59 pages. Available in PDF, EPUB and Kindle. Book excerpt: While switchgrass (Panicum virgatum (L.)) has long been recognized as a viable bioenergy feedstock, it and other plants have cell walls with recalcitrance to processing. Recalcitrance is recognized as a major barrier to broad adoption of switchgrass and other feedstocks for cellulosic bioenergy. In an effort to reduce recalcitrance, transgenic plants have been generated with altered cell wall phenotypes such as reduced lignin. Unfortunately, stable transformation of switchgrass and other C4 grasses is time intensive, costly, and genetic analysis is further complicated by polyploid genomic structures. Unlike switchgrass, which can be tetraploid to octoploid, a closely related species, Hall’s panicgrass (Panicum hallii Vasey), is diploid, and has a much smaller genome. In addition, Hall’s panicgrass is a smaller plant with a faster generation time and is capable of self-fertilization. In the present study, germplasm from two inbred populations of Hall’s panicgrass, FIL2 and HAL2, were selected to assess the feasibility of using Hall’s panicgrass as a model for switchgrass. Included in this work was the development of methods using seeds immediately harvested from plants grown in the greenhouse for germination, sterilization, callus induction, transformation, and regeneration. Seed germination was optimized on NB medium at 70 ±[plus or minus] 11% for FIL2 and 82 ±[plus or minus]3.0% for HAL2. Callus induction was optimized on MS-OG medium at 51 ±[plus or minus]29% and 81 ±[plus or minus]19% for HAL2. Shoot regeneration was optimized on REG medium at 11.5± [plus or minus] 0.8 shoots/gram for FIL2 and 11.3 ±[plus or minus]0.6 shoots/gram for HAL2. Root regeneration occurred at 100% frequency for all callus expressing roots on Diet-MSO. In addition to a complete tissue culture system, a suspension culture system was also developed to more rapidly produce tissue for cell-based experiments. Cell suspensions of Hall’s panicgrass, both FIL2 and HAL2, generated more callus after 16 weeks of culture (141 ±[plus or minus] 22% for FIL2; 302 ±[plus or minus] 54% for HAL2) than the solid-medium culture system.
Download or read book Climate Change Photosynthesis and Advanced Biofuels written by Ashwani Kumar and published by Springer Nature. This book was released on 2020-08-31 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of fossil fuels results in rising CO2 and other greenhouse gas (GHG) emissions, causing global temperature rise and climate change that will negatively impact human health, the food supply, and eventually worsen hunger and misery. Presently, fossil fuels meet 88% of the energy demand, resulting in rising CO2/GHG emissions at alarming rates. The increased use of biofuels would help to mitigate climate change. Efficiently designing methods for the production of biofuels and plant-derived high-value products requires a deeper understanding of photosynthetic processes as a prerequisite for applying novel biotechnologies. Accordingly, this book provides ample information and a wealth of illustrative examples. The book’s eighteen richly illustrated chapters are divided into three thematic parts. I: Photosynthesis and Biomass Production under Changing Conditions, II: Microalgae and Engineered Crops for Production of Biofuels and High-value Products, and III: Genetic Resources and Engineering Methods to Improve Crop Plants. Readers will find the latest information on the molecular basis of photosynthetic processes in plants (including the regulatory principles that allow plants to maintain homeostasis under changing conditions), stress resistance and synthetic pathways. In addition, the basic principles of important biotechnologies, as well as examples of specially designed crops capable of growing under stress conditions with improved productivity, are presented. The book sets the course for future research in the field of biofuel development and production and provides both general and specific information for students, teachers, academic researchers, industrial teams, and general readers who are interested in new developments concerning the production of biofuels with value-added properties.
Download or read book Modification of Carbohydrate Active Enzymes in Switchgrass Panicum Virgatum L to Improve Saccharification and Biomass Yields for Biofuels written by Jonathan Duran Willis and published by . This book was released on 2016 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: The natural recalcitrance of plant cell walls is a major commercial hurdle for plant biomass to be converted into a viable energy source as alternative to fossil fuels. To circumvent this hurdle manipulation of carbohydrate enzymes active in the cellulose and hemicellulose portions of the plant cell wall can be utilized to improve feedstocks. Production of cellulolytic enzymes by plants have been evaluated for reducing the cost associated with lignocellulosic biofuels. Plants have successfully served as bioreactors producing bacterial and fungal glycosyl hydrolases, which have altered plant growth to improve saccharification. A bioprospecting opportunity lies with the utilization of insect glycosyl hydrolases for transgenic production in plants. Lessons learned from microbial hydrolase expression can be applied to insect hydrolase expression along with gene stacking to develop autohydrolysis plant lines. A step toward production of insect cellulases in plants was performed by insertion of the endoglucanase TcEG1 gene, from Tribolium castaneum, into switchgrass. Transgenic lines overexpressing TcEG1 produced a functional enzyme with an optimal alkaline pH activity of 12.0. Recalcitrance was assayed by performing saccharification analysis, in which one line was superior over non-transgenic control; this line also had reduced 9% lignin content. Transgenic lines developed narrow stems, although biomass yield was unchanged due to increased tiller number and cell wall thickness. Grasses contain a relatively high amount of glucoarabinoxylan in their cell walls, which cross links with lignin. By down-regulation of a uridine diphosphate arabinomutase (UAM) gene via RNAi, it was hypothesized that attenuated production of this carbohydrate transferase would increase saccharification of switchgrass biomass from a disruption of cross linking. Transgenic events showed a reduction in arabinose content (up to 58%) and altered arabino-side chains, however saccharification was unchanged. UAM transgenic switchgrass showed a red node phenotype, which could be in response to increased lignin biosynthesis. A model of UAMinfluenced cell wall interactions was proposed and will used to build hypotheses for future - omics research. In summary, switchgrass saccharification and biomass yield can be increased by introduction of carbohydrate active enzymes. Combination of presented transgenic lines with low-lignin germplasm could be utilized to further improve saccharification yield.
Download or read book Advances in crop biomass production based on multi omics approach written by Yin Li and published by Frontiers Media SA. This book was released on 2023-05-12 with total page 145 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biofuel Production written by Marco Aurelio Dos Santos Bernardes and published by BoD – Books on Demand. This book was released on 2011-09-15 with total page 610 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book aspires to be a comprehensive summary of current biofuels issues and thereby contribute to the understanding of this important topic. Readers will find themes including biofuels development efforts, their implications for the food industry, current and future biofuels crops, the successful Brazilian ethanol program, insights of the first, second, third and fourth biofuel generations, advanced biofuel production techniques, related waste treatment, emissions and environmental impacts, water consumption, produced allergens and toxins. Additionally, the biofuel policy discussion is expected to be continuing in the foreseeable future and the reading of the biofuels features dealt with in this book, are recommended for anyone interested in understanding this diverse and developing theme.
Download or read book Plant Cell Walls written by Peter Albersheim and published by Garland Science. This book was released on 2010-04-15 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant cell walls are complex, dynamic cellular structures essential for plant growth, development, physiology and adaptation. Plant Cell Walls provides an in depth and diverse view of the microanatomy, biosynthesis and molecular physiology of these cellular structures, both in the life of the plant and in their use for bioproducts and biofuels. Plant Cell Walls is a textbook for upper-level undergraduates and graduate students, as well as a professional-level reference book. Over 400 drawings, micrographs, and photographs provide visual insight into the latest research, as well as the uses of plant cell walls in everyday life, and their applications in biotechnology. Illustrated panels concisely review research methods and tools; a list of key terms is given at the end of each chapter; and extensive references organized by concept headings provide readers with guidance for entry into plant cell wall literature. Cell wall material is of considerable importance to the biofuel, food, timber, and pulp and paper industries as well as being a major focus of research in plant growth and sustainability that are of central interest in present day agriculture and biotechnology. The production and use of plants for biofuel and bioproducts in a time of need for responsible global carbon use requires a deep understanding of the fundamental biology of plants and their cell walls. Such an understanding will lead to improved plant processes and materials, and help provide a sustainable resource for meeting the future bioenergy and bioproduct needs of humankind.
Download or read book Biofuels Greenhouse Gas Mitigation and Global Warming written by Ashwani Kumar and published by Springer. This book was released on 2018-02-09 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: This timely book is a compilation of edited articles by distinguished international scientists discussing global warming, its causes as well as present and future solutions. Social and economic growth at global level is measured in terms of GDP, which requires energy inputs generally based on fossil fuel resources. These, however, are major contributors to increasing levels of CO2, causing 15 tonnes of green house gas emissions per capita. Renewable sources of energy offer an alternative to fossil fuels, and would help reduce this to the 2 tonnes of greenhouse gas emissions per capita per annum needed to achieve sustainable growth. As such, the book discusses the next-generation of biofuels and all related aspects, based on the editors’ significant investigations on biofuels over the last 30 years. It also presents the latest research findings from research work carried out by contemporary researchers. Presenting global biofuel perspectives, it examines various issues related to sustainable development of biofuels in the contexts of agriculture, forestry, industry and economic growth. It covers the 1st to 4th generation biofuels, as well as the status of biofuel resources and their potential in carbon neutral economy. Offering a comprehensive, state-of-art overview of current and future biofuels at local and global levels, this book appeals to administrators, policy makers, universities and research institutions.
Download or read book Neural Networks written by Raul Rojas and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: Neural networks are a computing paradigm that is finding increasing attention among computer scientists. In this book, theoretical laws and models previously scattered in the literature are brought together into a general theory of artificial neural nets. Always with a view to biology and starting with the simplest nets, it is shown how the properties of models change when more general computing elements and net topologies are introduced. Each chapter contains examples, numerous illustrations, and a bibliography. The book is aimed at readers who seek an overview of the field or who wish to deepen their knowledge. It is suitable as a basis for university courses in neurocomputing.
Download or read book Cellulose Reinforced Nanofibre Composites written by Mohammad Jawaid and published by Woodhead Publishing. This book was released on 2017-06-06 with total page 563 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cellulose-Reinforced Nanofibre Composites: Production, Properties and Applications presents recent developments in, and applications of, nanocellulose as reinforcement in composite and nanocomposite materials. Written by leading experts, the book covers properties and applications of nanocellulose, including the production of nanocellulose from different biomass resources, the usefulness of nanocellulose as a reinforcement for polymer and paper, and major challenges for successful scale-up production in the future. The chapters draw on cutting-edge research on the use of nanosized cellulose reinforcements in polymer composites that result in advanced material characteristics and significant enhancements in physical, mechanical and thermal properties. The book presents an up-to-date review of the major innovations in the field of nanocellulose and provides a reference material for future research in biomass based composite materials, which is timely due to the sustainable, recyclable and eco-friendly demand for highly innovative materials made from biomass. This book is an ideal source of information for scientific and industrial researchers working in materials science. Gathers together a broad spectrum of research on nanocellulose, with emphasis on the outstanding reinforcing potential when nanocellulose is included into a polymer matrix or as an additive to paper Demonstrates systematic approaches and investigations from processing, design, characterization and applications of nanocellulose Presents a useful reference and technical guide for nanocomposite materials R&D sectors, university academics and postgraduate students (Masters and PhD) and industrialists working in material commercialization
Download or read book Biofuel Production Technologies Critical Analysis for Sustainability written by Neha Srivastava and published by Springer Nature. This book was released on 2020-03-23 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: Production and utilization of sustainable energy toward maintaining a clean environment is a major challenge. At the same time, the continued depletion of fossil fuels and the global dependency on non-renewable fuels is a chief concern. Moreover, the long-term economic and environmental issues associated with the high utilization of fossil fuel, such as global warming, are also important, particularly in the context of the predicted increase in the global population to around 5 billion by 2050. In recent years, researchers have been investigating alternative, renewable fuels to replace fossil fuels. Of the various options, biofuels are especially attractive due to their low production costs and the fact that they are pollution free. Also known as transportation fuels, their energy is derived from biological resources or through the biological processes. Biofuels such as biohydrogen, biomethane, biogas, ethanol and butanol offer a number of advantages and can be economically produced from cellulosic biomass. As such, they can play a vital role in sustainably meeting future energy demands. Biofuels have the potential to become a global primary energy source, offering significant reductions in greenhouse gas emissions as well as opportunities to increase economic and social development in rural communities and reduce the problems associated with waste disposal. However, low yields and lack of process technology are some of the aspects that need to be addressed. This book offers an overview of existing biofuels and the technologies to solve the problems associated with their practical implementation. Evaluating the biofuel options and discussing the opportunities and risks in relation to resources, technologies, practices, markets and policy, it provides insights into the development of economically viable bioenergy industries.
