Download or read book The Technique of Fluorescent Antibodies and Its Application in Virology written by David Kirsh and published by . This book was released on 1964 with total page 16 pages. Available in PDF, EPUB and Kindle. Book excerpt: The following aspects of the fluorescent antibody technique in virology are discussed: The optical system -- microscope, light source and filters; Labelling procedure -- labelling globulin with fluorescein isothiocyanate; Direct and indirect staining methods; Staining of complement; and The identification and localization of virus.
Download or read book Fluorescent Antibody Techniques and Their Applications written by Akiyoshi Kawamura and published by . This book was released on 1977 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Rapid Virus Diagnosis written by P. S. Gardner and published by Butterworth-Heinemann. This book was released on 2014-06-28 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rapid Virus Diagnosis: Application of Immunofluorescence presents developments in immunofluorescence as a technique for the diagnosis of virus infections. It discusses the effective and rapid methods for the diagnosis of virus infections. It addresses the application of fluorescent antibody techniques to the diagnosis and the investigation of virus infections and the assessment of their value both to the clinician and to the virologist. Some of the topics covered in the book are the fluorescence microscopy; nature of fluorescence; filter systems; transmitted light microscopy; interference filters for transmitted light microscopy; lamp centration and alignment of microscope; contrast-fluorescence condensers; photomicrography; and choice of microscope. The culture of viruses for production of antiserum is fully covered. The selection of cell lines or tissues for virus culture is discussed in detail. The text describes in depth the fluorescent antibody staining techniques. The preparation of specimens from respiratory infections is presented completely. A chapter is devoted to the respiratory syncytial virus. Another section focuses on the analysis influenza virus, paramyxoviruses, and picornaviruses. The book can provide useful information to doctors, virologists, students, and researchers.
Download or read book Fluorescent Antibody Methods written by Morris Goldman and published by . This book was released on 1968 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence generalities; Light sources; Filter systems; Microscopy; Preparation and fractionation of antiserum; Labeling agents and procedures for conjugation; Properties of conjugated sera; Cytological and histological methods; Staining methods; Unwanted fluorescence; Applications of fluorescent antibody.
Download or read book Global Infectious Disease Surveillance and Detection written by Institute of Medicine and published by National Academies Press. This book was released on 2007-11-11 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Early detection is essential to the control of emerging, reemerging, and novel infectious diseases, whether naturally occurring or intentionally introduced. Containing the spread of such diseases in a profoundly interconnected world requires active vigilance for signs of an outbreak, rapid recognition of its presence, and diagnosis of its microbial cause, in addition to strategies and resources for an appropriate and efficient response. Although these actions are often viewed in terms of human public health, they also challenge the plant and animal health communities. Surveillance, defined as "the continual scrutiny of all aspects of occurrence and spread of a disease that are pertinent to effective control", involves the "systematic collection, analysis, interpretation, and dissemination of health data." Disease detection and diagnosis is the act of discovering a novel, emerging, or reemerging disease or disease event and identifying its cause. Diagnosis is "the cornerstone of effective disease control and prevention efforts, including surveillance." Disease surveillance and detection relies heavily on the astute individual: the clinician, veterinarian, plant pathologist, farmer, livestock manager, or agricultural extension agent who notices something unusual, atypical, or suspicious and brings this discovery in a timely way to the attention of an appropriate representative of human public health, veterinary medicine, or agriculture. Most developed countries have the ability to detect and diagnose human, animal, and plant diseases. Global Infectious Disease Surveillance and Detection: Assessing the Challenges-Finding Solutions, Workshop Summary is part of a 10 book series and summarizes the recommendations and presentations of the workshop.
Download or read book Fluorescence Microscopy for Disease Diagnosis and Environmental Monitoring written by Warren R. Sanborn and published by . This book was released on 2005 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laboratories should be more aware of the advantages of using fluorescence microscopy. This manual provides information on the principles of fluorescence microscopy and practical advice on the preparation of samples for many simple applications for diagnosing disease and monitoring environmental contamination using a fluorescence microscope. The publication puts emphasis on procedures for direct, rapid identification of microorganisms causing a disease. The practical steps of indirect immunofluorescence microscopy for the diagnosis of noncommunicable diseases are also considered.
Download or read book Immunofluorescence Methods in Virology written by H. Craig Lyerla and published by . This book was released on 1979 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science. Serves as a helpful guide for clinical researchers who lack a conventional science background Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
Download or read book Cerebral Palsy in Infancy written by Roberta B. Shepherd and published by Elsevier Health Sciences. This book was released on 2013-09-20 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cerebral Palsy in Infancy is a thought-provoking book which introduces a new way of thinking on the development and use of interventions. Relevant to current practice, it advocates early, targeted activity that is focused on increasing muscle activation, training basic actions and minimizing (or preventing) mal-adaptive changes to muscle morphology and function. The authors present recent scientific findings in brain science, movement sciences (developmental biomechanics, motor control mechanisms, motor learning, exercise science) and muscle biology. This knowledge provides the rationale for active intervention, underpinning the need for an early referral to appropriate services. The book features methods for promoting relatively intensive physical activity in young infants without placing a burden on parents which include assistive technologies such as robotics, electronic bilateral limb trainers and baby treadmills. Cerebral Palsy in Infancy begins by specifying the guidelines for training and exercise, outlining the rationale for such intervention. It goes on to cover the fundamentals of neuromotor plasticity and the development and negative effects of limited motor activity on brain organization and corticospinal tract development. Neuromuscular adaptations to impairments and inactivity are discussed along with the General Movement assessment that can provide early diagnosis and prognosis, facilitating very early referral from paediatric specialists to training programs. The book ends with a section featuring various methods of training with the emphasis on preventing/minimizing muscle contracture, stimulating biomechanically critical muscle activity and joint movement. An ideal clinical reference for those working to improve the lives of infants suffering from cerebral palsy. CONTRIBUTORS: Adel Abdullah Alhusaini (Saudi Arabia); David I. Anderson (USA); Nicolas Bayle (France); Roslyn Boyd (Australia); Giovanni Cioni (Italy); Diane L. Damiano (USA); Janet Eyre (UK); Linda Fetters (USA); Mary Galea (Australia); Andrew M Gordon (USA); Martin Gough (UK); Richard L Lieber (USA); Jens Bo Nielsen (Denmark); Micah Perez (Australia); Caroline Teulier (France). "This book provides a comprehensive overview of the challenges of motor development and the consequent impact of poor motor function in later childhood for infants with cerebral palsy (CP)."Reviewed by: Oxford Brookes University on behalf of the British Journal of Occupational Therapy, Dec 2014 conceived and edited by Roberta Shepherd with contributions from internationally renowned expert clinicians and researchers discusses new research and new evidence-based treatment interventions shows how to organize very early and intensive physical activity in young infants to stimulate motor development and growth therapies include the specificity of training and exercise, with emphasis on promoting muscle activity and preventing contracture by active instead of passive stretching methods include new interactive technologies in enhancing home-based training sessions carried out by the infant's family extensive referencing in each chapter for further study chapters feature "Annotations" which illustrate scientific findings
Download or read book Laboratory Diagnosis of Infectious Diseases Principles and Practice written by Edwin H. Lennette and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 983 pages. Available in PDF, EPUB and Kindle. Book excerpt: those who deal with infectious diseases on a daily This two volume work stems from the belief of the Editors that infectious diseases are not only very basis. much with us today but, more importantly, that they There are several excellent textbooks dealing will continue to playa significant global role in mor with medical microbiology, and there are equally bidity and mortality in all people. A continuing need well-recognized books devoted to infectious dis for an informed and knowledgeable community of eases. The Editors of this work, on the other hand, were persuaded that there was a need for a publica laboratory scientists is fundamental. Data describing tion that would bring together the most pertinent and the global impact of infectious diseases are difficult to come by. Fortunately, a recent thoughtful and relevant information on the principles and practice of provocative publication by Bennett et al. (1987) pro the laboratory diagnosis of infectious diseases and vides us with data derived from several consultants include clinical relationships. While this two volume that clearly delineate the impact of infectious dis text is directed toward the role of the laboratory in eases on the United States today.
Download or read book Far Field Optical Nanoscopy written by Philip Tinnefeld and published by Springer. This book was released on 2015-02-07 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes developments in the field of super-resolution fluorescence microscopy or nanoscopy. In 11 chapters, distinguished scientists and leaders in their respective fields describe different nanoscopy approaches, various labeling technologies, and concrete applications. The topics covered include the principles and applications of the most popular nanoscopy techniques STED and (f)PALM/STORM, along with advances brought about by fluorescent proteins and organic dyes optimized for fluorescence nanoscopy. Furthermore, the photophysics of fluorescent labels is addressed, specifically for improving their photoswitching capabilities. Important applications are also discussed, such as the tracking and counting of molecules to determine acting forces in cells, and quantitative cellular imaging, respectively, as well as the mapping of chemical reaction centers at the nano-scale. The 2014 Chemistry Nobel Prize® was awarded for the ground-breaking developments of super-resolved fluorescence microscopy. In this book, which was co-edited by one of the prize winners, readers will find the most recent developments in this field.
Download or read book Neuroanatomical Tract Tracing written by Laszlo Zaborszky and published by Springer Science & Business Media. This book was released on 2006-11-22 with total page 703 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first two editions of this title had a tremendous impact in neuroscience. Between the Second edition in 1989 and today, there has been an explosion of information in the field, including advances in molecular techniques, such as genomics and proteomics, which have become increasing important in neuroscience. A renaissance in fluorescence has occurred, driven by the development of new probes, new microscopes, live imagers, and computer processing. The introduction of new markers has enormously stimulated the field, moving it from tissue culture to neurophysiology to functional MRI techniques.
Download or read book Immunohistochemistry written by Alexander E. Kalyuzhny and published by Springer. This book was released on 2016-03-24 with total page 91 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aimed at both beginners and experienced researchers unfamiliar with the technique, this book provides a concise and easy-to-understand guide to using immunohistochemistry. It identifies the proper place and purpose of each component of immunohistochemistry, emphasizes the components that are of critical importance, and explains the logistics of experimental approaches. Understanding the principles of immunohistochemistry allows for the identification and localization of protein targets and is of crucial use in the following experiments: · producing transgenic animals · studying the effects of drug treatments · electrophysiological studies · animal surgery · isolating and differentiating stem cells · super-resolution microscopy Immunohistochemistry for the Non-Expert is aimed at a wide audience, including molecular biologists, pharmacologists, immunologists, chemists, cell biologists, physiologists, and researchers involved with animal anatomy studies.
Download or read book Canine and Feline Infectious Diseases E BOOK written by Jane E. Sykes and published by Elsevier Health Sciences. This book was released on 2013-06-06 with total page 928 pages. Available in PDF, EPUB and Kindle. Book excerpt: Canine and Feline Infectious Diseases is a practical, up-to-date resource covering the most important and cutting-edge advances in the field. Presented by a seasoned educator in a concise, highly visual format, this innovative guide keeps you current with the latest advances in this ever-changing field. 80 case studies illustrate the clinical relevance of the major infectious disease chapters. Well-organized Major Infectious Diseases chapters break down content by etiologic agent and epidemiology, clinical signs and their pathophysiology, physical examination findings, diagnosis, treatment and prognosis, immunity, prevention, and public health implications. Over 80 case studies illustrate how the information provided can be applied in everyday practice. Logical approach to laboratory diagnosis guides you through all the steps needed to accurately diagnose and treat viral, bacterial, fungal, protozoal, and algal diseases. Practical protocols provided by expert clinicians guide you in the management of canine and feline patients suspected to have infectious diseases, including handling, disinfection, isolation, and vaccination protocols. Over 500 full color images – geographic distribution maps, life cycle drawings, and hundreds of color photographs – visually illustrate and clarify complex issues. Easy-to-understand tables and boxes make content quickly accessible, eliminating the need to sort through dense text for critical information in the clinical setting.
Download or read book Flow Cytometry and Cell Sorting written by Andreas Radbruch and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS
Download or read book Recent Advances in Virus Diagnosis written by M. S. McNulty and published by . This book was released on 1984 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Laboratory Guide to the Tight Junction written by Jianghui Hou and published by Academic Press. This book was released on 2020-04-24 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models. Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research. Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases. Provides consistent and detailed research methods Covers various cell, tissue and animal models Includes step-by-step guidance from beginner to sophisticated levels
