Download or read book The LINC Complex written by Gregg G. Gundersen and published by . This book was released on 2018 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The LINC Complex written by Gregg G. Gundersen and published by Humana. This book was released on 2019-09-07 with total page 338 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes research methodologies and approaches used to study the Linker of Nucleoskeleton and Cytoskeleton (LINC) complex and its cellular functions. Chapters detail structural and biochemical analysis of LINC complexes, mechanical aspects of the LINC complex, analysis of the LINC complex in model systems and development, and LINC complex in mammalian tissue, organs, and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The LINC Complex: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Download or read book Structural Studies on the LINC Complex and Fic 1 written by Xuanzong Guo and published by . This book was released on 2015 with total page 38 pages. Available in PDF, EPUB and Kindle. Book excerpt: LINC complexes span the nuclear envelope and connect the nucleoskeleton to the cytoskeleton. In 2012, our lab solved the first LINC complex structure, that of SUN domain of human SUN2 bound with KASH1 or KASH2 peptides. In this project testes-specific human SUN proteins (SUN3, SPAG4, and SUNS) were compared to ubiquitously-expressed SUN2. Secondly, fission and budding yeast LINC complexes differ from human ones and were analyzed as well. I was able to confirm SUN-KASH interaction in human and yeast. For structural analysis I explored various expression strategies. Fic-1 is a C. elegans Fic-domain protein with diverse cellular functions. As a subfamily III Fic enzyme, Fic-1 may reveal valuable insights into Fic enzyme mechanisms from its structure. After trying different knowledge-informed constructs and crystal optimization, small Fic-1 crystals were obtained, which diffracted X-rays to ~ 7 [angstroms]. With modest additional effort diffraction-quality crystals should be achievable.
Download or read book Molecular Basis of Linkers of the Nucleoskeleton and Cytoskeleton Complex Formation written by Brian Alexander Sosa-Alvarado and published by . This book was released on 2014 with total page 111 pages. Available in PDF, EPUB and Kindle. Book excerpt: The nucleus is the hallmark of the eukaryotic cell. It contains most of the genetic material and it separates the processes of replication and transcription from that of translation. Communication between the nucleus and the cytoplasm occurs mostly through openings in the nuclear envelope composed of nuclear pore complexes. These massive assemblies allow for regulated transport of macromolecules across the barrier that is the nuclear envelope. However, another means of communication between the nucleus and the cytoskeleton has been characterized: linkers of the nucleoskeleton and cytoskeleton, or LINC, complexes mechanically connect the nucleus with its surroundings allowing for nuclear anchorage and nuclear movement during development and chromosome movement during meiosis. At the heart of LINC complexes are inner nuclear membrane resident SUN proteins and outer nuclear membrane resident KASH proteins. In this thesis we structurally characterize the human SUN2-KASH1 and KASH2 complexes and provide the molecular basis for their interaction. The solved structures suggest plausible models for high-order of LINC complex assembly as well as LINC complex mediated spacing between the inner and outer nuclear membrane. Questions about how these complexes are regulated also arise from the structure. TorsinA is an AAA+ ATPase suggested to play a role in LINC complex regulation. Analysis of Torsin's binding partners LAP1 and LULL1 show that they are catalytically inactive AAA+ ATPases. We characterize the complex and show by EM that they form ring akin to other AAA+ ATPases. With these studies we provide the first structural analysis of LINC complexes and Torsin ATPases and also provide biochemical tools for the study of LINC complex regulation.
Download or read book The Extracellular Matrix written by Davide Vigetti and published by Humana Press. This book was released on 2019-03-31 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details established experimental protocols on the isolation, characterization, and detection of extracellular matrix (ECM) molecules as well as methods to study the activity and role of ECM components on various biological functions, the formation of exosomes, and tissue surrogates. Chapters display a variety of protocols ranging from biochemical, cell, and molecular biology assays to complex tissue imaging techniques and in vivo models to elucidate the role of the extracellular matrix. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Extracellular Matrix: Methods and Protocols aims to provide protocols and applications that can be used by both Ph.D. students and senior researchers to advance research in the field of extracellular matrix biology.
Download or read book Cellular Migration and Formation of Neuronal Connections written by and published by Academic Press. This book was released on 2013-05-06 with total page 1081 pages. Available in PDF, EPUB and Kindle. Book excerpt: The genetic, molecular, and cellular mechanisms of neural development are essential for understanding evolution and disorders of neural systems. Recent advances in genetic, molecular, and cell biological methods have generated a massive increase in new information, but there is a paucity of comprehensive and up-to-date syntheses, references, and historical perspectives on this important subject. The Comprehensive Developmental Neuroscience series is designed to fill this gap, offering the most thorough coverage of this field on the market today and addressing all aspects of how the nervous system and its components develop. Particular attention is paid to the effects of abnormal development and on new psychiatric/neurological treatments being developed based on our increased understanding of developmental mechanisms. Each volume in the series consists of review style articles that average 15-20pp and feature numerous illustrations and full references. Volume 2 offers 56 high level articles devoted mainly to Formation of Axons and Dendrites, Migration, Synaptogenesis, Developmental Sequences in the Maturation of Intrinsic and Synapse Driven Patterns. - Series offers 144 articles for 2904 full color pages addressing ways in which the nervous system and its components develop - Features leading experts in various subfields as Section Editors and article Authors - All articles peer reviewed by Section Editors to ensure accuracy, thoroughness, and scholarship - Volume 2 sections include coverage of mechanisms which regulate: the formation of axons and dendrites, cell migration, synapse formation and maintenance during development, and neural activity, from cell-intrinsic maturation to early correlated patterns of activity
Download or read book Mechanotransduction written by and published by Academic Press. This book was released on 2014-07-29 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: Progress in Molecular Biology and Translational Science provides a forum for discussion of new discoveries, approaches, and ideas in molecular biology. It contains contributions from leaders in their fields and abundant references. Volume 126 features in-depth reviews that focus on the tools required to investigate mechanotransduction. Additional chapters focus on how we can use these tools to answer fundamental questions about the interaction of physical forces with cell biology, morphogenesis, and function of mature structures. Chapters in the volume are authored by a unique combination of cell biologists and engineers, providing a range of perspectives on mechanotransduction. - Provides a unique combination of perspectives from biologists and engineers - Engaging to people of many training backgrounds
Download or read book Cancer Biology and the Nuclear Envelope written by Eric C. Schirmer and published by Springer Science & Business Media. This book was released on 2014-02-22 with total page 610 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Nuclear envelope (NE) defects have been linked to cancer biology since the mid-1800s, but it was not until the last few years that we have begun to understand these historical links and to realize that there are myriad ways that the NE impacts on tumorigenesis. The NE is a complex double membrane system that encloses the genome while providing structural support through the intermediate filament lamin polymer and regulating protein/ mRNA trafficking and signaling between the nucleus and cytoplasm via the nuclear pore complexes (NPCs). These functions already provide some mechanisms for NE influences on cancer biology but work in the past few years has elucidated many others. Lamins and many recently identified NE transmembrane proteins (NETs) have been now shown to function in DNA repair, regulation of cell cycle and signaling, apoptosis, cell migration in metastasis and nuclear architecture and morphology. This volume presents a comprehensive overview of the wide range of functions recently identified for NE proteins and their relevance in cancer biology, providing molecular mechanisms and evidence of their value as prognostic and diagnostic markers and suggesting new avenues for the treatment of cancer. Indeed some of these recent links are already yielding promising therapies, such as the current clinical trial of selective inhibitors of the nuclear export factor exportin in certain types of leukemia, melanoma and kidney cancer."
Download or read book Chasing Lincoln s Killer written by James L. Swanson and published by Scholastic Inc.. This book was released on 2012-09-01 with total page 249 pages. Available in PDF, EPUB and Kindle. Book excerpt: NEW YORK TIMES bestselling author James Swanson delivers a riveting account of the chase for Abraham Lincoln's assassin. Based on rare archival material, obscure trial manuscripts, and interviews with relatives of the conspirators and the manhunters, CHASING LINCOLN'S KILLER is a fast-paced thriller about the pursuit and capture of John Wilkes Booth: a wild twelve-day chase through the streets of Washington, D.C., across the swamps of Maryland, and into the forests of Virginia.
Download or read book Dynamics of the LINC Complex written by Loic Gazquez and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Intermediate Filament Proteins written by and published by Academic Press. This book was released on 2016-01-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Intermediate Filament Proteins, the latest volume in the Methods in Enzymology series covers all the intermediate filaments in vertebrates and invertebrates, providing a unique understanding of the multiple different tissue-specific intermediate filaments. This volume also covers the latest methods that are currently being used to study intermediate filament protein function and dynamics. It will be an important companion for any experimentalist interesting in studying this protein family in their cell or organism model system.
Download or read book The Liver written by Irwin M. Arias and published by John Wiley & Sons. This book was released on 2020-03-09 with total page 1156 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bridging the gap between basic scientific advances and the understanding of liver disease — the extensively revised new edition of the premier text in the field. The latest edition of The Liver: Biology and Pathobiology remains a definitive volume in the field of hepatology, relating advances in biomedical sciences and engineering to understanding of liver structure, function, and disease pathology and treatment. Contributions from leading researchers examine the cell biology of the liver, the pathobiology of liver disease, the liver’s growth, regeneration, metabolic functions, and more. Now in its sixth edition, this classic text has been exhaustively revised to reflect new discoveries in biology and their influence on diagnosing, managing, and preventing liver disease. Seventy new chapters — including substantial original sections on liver cancer and groundbreaking advances that will have significant impact on hepatology — provide comprehensive, fully up-to-date coverage of both the current state and future direction of hepatology. Topics include liver RNA structure and function, gene editing, single-cell and single-molecule genomic analyses, the molecular biology of hepatitis, drug interactions and engineered drug design, and liver disease mechanisms and therapies. Edited by globally-recognized experts in the field, this authoritative volume: Relates molecular physiology to understanding disease pathology and treatment Links the science and pathology of the liver to practical clinical applications Features 16 new “Horizons” chapters that explore new and emerging science and technology Includes plentiful full-color illustrations and figures The Liver: Biology and Pathobiology, Sixth Edition is an indispensable resource for practicing and trainee hepatologists, gastroenterologists, hepatobiliary and liver transplant surgeons, and researchers and scientists in areas including hepatology, cell and molecular biology, virology, and drug metabolism.
Download or read book Tissue Morphogenesis written by Celeste M. Nelson and published by Springer Nature. This book was released on with total page 235 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Yeast Nucleus written by Peter Fantes and published by . This book was released on 2000 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Thunderhead written by Douglas Preston and published by Grand Central Publishing. This book was released on 2001-07-01 with total page 445 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nora Kelly, a young archaeologist in Santa Fe, receives a letter written sixteen years ago, yet mysteriously mailed only recently. In it her father, long believed dead, hints at a fantastic discovery that will make him famous and rich---the lost city of an ancient civilization that suddenly vanished a thousand years ago. Now Nora is leading an expedition into a harsh, remote corner of Utah's canyon country. Searching for her father and his glory, Nora begins t unravel the greatest riddle of American archeology. but what she unearths will be the newest of horrors...
Download or read book Lincoln Center Inside Out written by Diller Scofidio + Renfro and published by Damiani Limited. This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The redesign of Lincoln Center is one of the most challenging and innovative civic projects in recent urban history. Over the past eight years Diller Scofi dio + Renfro, in close collaboration with Lincoln Center's leadership, has transformed the fi fty year old Modernist citadel into a porous and democratic campus. This visually rich document is the first comprehensive book to feature the extensive redevelopment in its entirety. Through a combination of photographs, drawings, renderings, archival records and texts, the book describes the innovative strategies that have dissolved the public/private divide and effectively turned the campus inside-out, extending the spectacle of the performance halls into the Center's mute public spaces and surrounding streets. Conceived as a cross between an art book, a scholarly record, and an architectural diary this publication demonstrates how the recent redesign both respects and challenges preconceived notions about Lincoln Center and its ongoing role as a cultural hub in an ever-changing city. This unorthodox publication is comprised entirely of gatefolds; a series of inside-out centerfolds where the exterior pages of each spread feature glossy, large-format, full-bleed photographs highlighting different parts of the campus. Inside the gatefolds, tucked behind these lush photos, is a series of "back stories" that reveal the surprising evolution and unexpected afterlife of the same spaces.
Download or read book Cellular Mechanotransduction written by Mohammad R. K. Mofrad and published by Cambridge University Press. This book was released on 2014-07-31 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Mechanotransduction" is the term for the ability, first described by 19th-century anatomist Julius Wolff, of living tissues to sense mechanical stress and respond by tissue remodeling. More recently, the scope of mechanotransduction has been expanded to include the sensation of stress, its translation into a biochemical signal, and the sequence of biological responses it produces. This book looks at mechanotransduction in a more restricted sense, focusing on the process of stress sensing and transducing a mechanical force into a cascade of biochemical signals. This stress has become increasingly recognized as one of the primary and essential factors controlling biological functions, ultimately affecting the function of the cells, tissues, and organs. A primary goal of this broad book is also to help define the new field of mechanomics, which attempts to describe the complete mechanical state of a biological system.
