Download or read book Stress Protein Expression and Metabolism in the Heart of the Rainbow Trout written by Judah Goldstein and published by . This book was released on 2005 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Heat Shock Protein Expression in the Rainbow Trout Oncorhynchus Mykiss Heart in Response to Separate Heat and Hypoxic Stress written by Eryn Creamer and published by . This book was released on 2007 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Effects of Environmental Stressors on Cardiac Gene Expression and Contractility in Rainbow Trout Oncorhynchus Mykiss written by Tracey S. Momoda and published by . This book was released on 2014 with total page 114 pages. Available in PDF, EPUB and Kindle. Book excerpt: Rainbow trout (Oncorhynchus mykiss) experience a variety of stressors in their natural environment as well as in aquaculture that can have negative effects on their physiology. The effects of physiological stress and endocrine disruption have been well described in fish. However, there is a scarcity of information on the effects of such stressors on the heart in fish. We examined gene expression in the rainbow trout heart in response to 17[alpha]-ethinylestradiol (EE), the active ingredient in some oral contraceptives, as well as physiological stress. We also assessed contractile function, as a measure of cardiac performance, under conditions of increasing performance demands in sexually immature rainbow trout. In chapter 2, using qRT-PCR, we observed increased vitellogenin (vtg1) in the ventricle, along with, hepatic estrogen receptor (ER[alpha]) and vtg1 mRNA increased with EE exposure, confirming endocrine disruption in both tissues. Stress increased corticotropin-releasing factor receptor 1 (CRF-R1) and JunB in response to stress while nuclear protein 1 (nupr1) and glucocorticoid receptor (GCR2) expression increased only in the population with higher cortisol levels (>100 ng/ml) in response to stress. Interestingly, EE exposure did not affect stress induced gene expression and stress did not have an effect on EE induced gene expression in the heart of rainbow trout. This research highlights the fact that these stressors have the potential to have physiological impacts beyond the target tissues classically thought of for stress (i.e. HPI/A axis) or exposure to estrogenic compounds (i.e. HPG axis). In chapter 3, contractile force production was measured from ventricle strips exposed to increasing concentrations of Ca2 or epinephrine to simulate conditions of increasing performance. Also, to determine the effects of physiological stress, ventricle strips were pre-treated with 100 ng/L cortisol prior to increasing Ca2 or epinephrine. Ventricle strips from males had a greater contractile force production to increasing Ca2+ or epinephrine compared to females, at concentrations greater than 3.5 mM Ca2+ or 1 [micro]M epinephrine. Exposure to cortisol did not have a direct effect on contractility, nor did it have an effect the contractile response to increasing exogenous Ca2+ in either sex. Interestingly, force production did not vary by sex in the cortisol pre-treated exposed to epinephrine, suggesting a sex-dependent response. My findings suggest that there are sex differences in the contractile function in rainbow trout and that these may be modified under conditions related to physiological stress. The rainbow trout is a genetically diverse species found across a variety of habitats. Our results highlight the variation in responses in in contractile function and gene expression in the heart by the different sexes, as well as variation within and among populations that allow rainbow trout to adapt and perform in the presence of stressors in the environment.
Download or read book Biology of Stress in Fish written by Carl B. Schreck and published by Academic Press. This book was released on 2016-11-01 with total page 604 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biology of Stress in Fish: Fish Physiology provides a general understanding on the topic of stress biology, including most of the recent advances in the field. The book starts with a general discussion of stress, providing answers to issues such as its definition, the nature of the physiological stress response, and the factors that affect the stress response. It also considers the biotic and abiotic factors that cause variation in the stress response, how the stress response is generated and controlled, its effect on physiological and organismic function and performance, and applied assessment of stress, animal welfare, and stress as related to model species. Provides the definitive reference on stress in fish as written by world-renowned experts in the field Includes the most recent advances and up-to-date thinking about the causes of stress in fish, their implications, and how to minimize the negative effects Considers the biotic and abiotic factors that cause variation in the stress response
Download or read book Heat Shock Proteins and Physiological Stress in Redband Rainbow Trout Oncorhynchus Mykiss Gairdneri in the South Fork John Day River Oregon written by Joseph W. Feldhaus and published by . This book was released on 2006 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goal of this research was to characterize thermal habitat requirements for juvenile redband steelhead trout (Oncorhynchus mykiss gairdneri) in the South Fork John Day River (SFJD), Oregon using physiological indicators of fish condition. Physiological indices of fish condition measured were whole body lipid content and heat shock proteins, specifically hsp70. Hsp70 is a family of highly conserved molecular chaperones proteins that protect cellular function by preventing irreversible protein damage. Hsp70 levels were measured in liver, white muscle, and fin tissue. This thesis includes a study of passive integrated transponder (PIT) tag handling stress in juvenile rainbow trout. PIT tags are used to identify individual fish. The objectives of the PIT tag study were to determine if there is a stress response, measured as a difference in plasma cortisol levels, between PIT tagged and non-PIT tagged rainbow trout (O. mykiss), to determine if PIT tag-related stress induces hepatic hsp70 synthesis in juvenile rainbow trout, and to examine the relation between cortisol and hsp70 levels. There was a difference in plasma cortisol six hours after tagging, with both treatment and tank effects. There were no differences detected after the 24h sampling period. Plasma cortisol levels were highly variable, but changes in plasma cortisol did not alter hepatic hsp70 levels. A laboratory experiment demonstrated that hsp70 levels increase significantly between 19 and 22ðC in both fin and liver tissue. The finding that hsp70 can be quantified in fin tissue is significant because it provides a non-lethal technique for assessing thermal stress in rare or endangered fish. The response of hsp70 in relation to temperature was sigmoid. During the summers of 2004 and 2005, a field study in the SFJD was conducted. The objectives were to: (1) determine if SFJD redband rainbow trout experience thermal stress, (2) develop a non-lethal technique for measuring cellular hsp70 levels, (3) and characterize the relation between whole body lipids and hsp70 for fish in the SFJD. Maximum hsp70 levels in liver and white muscle tissue in field collected fish occurred when mean weekly maximum temperatures (MWMT) exceeded 22-23ðC. Short and long term stream temperature averages of 15.6-18.2ðC and temperature maximums of 18.8-21.6ðC significantly increased hsp70 levels in liver tissue. Both the hsp72 and hsp73 isoforms were significantly elevated in white muscle tissue when long and short term average stream temperatures were 16-18.5ðC and 19.6-23ðC for temperature maximums. Lipid levels began to decrease when MWMT exceeded 23ðC. Results of this research suggest increased cellular hsp70 levels in juvenile redband rainbow trout in the SFJD River is symptomatic of cellular stress related to thermal conditions, as are decreasing lipid levels. Measurement of cellular hsp70 levels provides another tool that can be used to characterize physiological suitability of thermal habitat and potentially to define thermal limits, as differences of 1 or 2°C are sufficient to change expression of thermal stress proteins detected using these techniques. When using hsp70 as an index of thermal stress in different O. mykiss subpopulations, differences in hsp70 expression between tissues should be considered.
Download or read book Amino Acids Regulate Hepatic Intermediary Metabolism related Gene Expression Via MTORC1 dependent Manner in Rainbow Trout Oncorhynchus Mykiss written by Dai Weiwei and published by . This book was released on 2015 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: During my doctoral study, we used rainbow trout, a representative carnivorous fish and relevant diabetic model, to study the mechanisms underlying the regulation of hepatic intermediary metabolism by nutrients (amino acids (AAs) and glucose), and determine the potential involvement of insulin/Akt and mTORC1 signaling pathways in these regulations. Using acute administration of rapamycin, a pharmacological inhibitor of TOR, we first identified that mTORC1 activation promotes the expression of genes related to fatty acid biosynthesis, glycolysis and amino acid catabolism, while Akt negatively regulates gluconeogenic gene expression in rainbow trout liver and primary hepatocytes. Furthermore, we demonstrated hepatic fatty acid biosynthetic gene expression is more responsive to dietary protein intake/AAs than dietary carbohydrate intake/glucose during acute stimulations in vivo and in vitro. Moreover, we further showed that high levels of AAs up-regulate hepatic fatty acid biosynthetic gene expression through an mTORC1-dependent manner, while excessive AAs attenuate insulin-mediated repression of gluconeogenesis through elevating IRS-1 Ser302 phosphorylation, which in turn impairs Akt phosphorylation and dampens insulin action. Finally, using glucose tolerance test and acute inhibition of rapamycin, we concluded that hepatic gluconeogenesis probably plays a major role in controlling glucose homeostasis, which maybe account for the prolonged hyperglycemia and glucose intolerance phenotype of carnivorous fish. The present thesis brings forward our understandings about the roles of protein/AAs in the regulation of hepatic intermediary metabolism in trout and identifies relevant cellular signaling pathways mediating the action of amino acids on metabolism. It also clarifies some nutritional characteristics of the trout.
Download or read book Effects of Maturation Polyploidy and Nutrition on Growth Composition and Gene Expression Within Fatty Acid Metabolism in Rainbow Trout written by Meghan Lynn Manor and published by . This book was released on 2014 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cumulated Index Medicus written by and published by . This book was released on 2000 with total page 1840 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Master s Theses Directories written by and published by . This book was released on 2006 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Education, arts and social sciences, natural and technical sciences in the United States and Canada".
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 1504 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vols. for 1963- include as pt. 2 of the Jan. issue: Medical subject headings.
Download or read book The Journal of Experimental Biology written by and published by . This book was released on 2008 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Swimming Physiology of Fish written by Arjan P. Palstra and published by Springer Science & Business Media. This book was released on 2012-08-30 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: In light of mounting fishing pressures, increased aquaculture production and a growing concern for fish well-being, improved knowledge on the swimming physiology of fish and its application to fisheries science and aquaculture is needed. This book presents recent investigations into some of the most extreme examples of swimming migrations in salmons, eels and tunas, integrating knowledge on their performance in the laboratory with that in their natural environment. For the first time, the application of swimming in aquaculture is explored by assessing the potential impacts and beneficial effects. The modified nutritional requirements of “athletic” fish are reviewed as well as the effects of exercise on muscle composition and meat quality using state-of-the-art techniques in genomics and proteomics. The last chapters introduce zebrafish as a novel exercise model and present the latest technologies for studying fish swimming and aquaculture applications.
Download or read book Immunomodulators in Aquaculture and Fish Health written by Preetham Elumalai and published by CRC Press. This book was released on 2023-10-31 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference book provides updated information about different immunomodulators for managing fish health and sustainable aquaculture. Immunomodulators are dietary additives that enhance innate defense mechanisms and increase resistance against specific pathogens and diseases. The book covers the different types of immunostimulants, their modes of action, and their efficacies. It also reviews safety concerns, ethical regulations, limitations, and outreach to farmers. It discusses the application of herbal immunomodulators, antioxidants, pre- and pro-biotics, in disease management. Features: • Reviews the pressing topic of reduction of antibiotic use in aquaculture • Discusses herbal immunomodulators, nutrients, antioxidants and pre- and pro-biotics • Covers the topic of progressive immunomodulation using nanotechnology • Discusses fish health management in the ever-growing aquaculture industry • Includes natural and synthetic immunomodulators The book is meant for researchers and industry experts in aquaculture, fisheries science, and veterinary medicine.
Download or read book Journal of Experimental Biology written by and published by . This book was released on 2006 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Difference Gel Electrophoresis written by Kay Ohlendieck and published by Springer Nature. This book was released on 2022-11-15 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of this volume provides a comprehensive update of this key method on gel-based proteomics. Chapters present an introduction into the development of methods on principles of differential protein labeling and two-dimensional gel electrophoresis, techniques on optimized proteomic workflows using advanced mass spectrometry for protein identification, and the application of those methods in basic biological research, pathobiology and applied biomarker discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Difference Gel Electrophoresis: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.
Download or read book The Physiology of Fishes written by Suzanne Currie and published by CRC Press. This book was released on 2020-09-07 with total page 285 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fifth edition of The Physiology of Fishes represents a compendium of knowledge across fish physiology, collecting up-to-date research into an easy-to-access single textbook. Written by the leaders in the field, it provides a comprehensive, accessible review of the core topics, integrating physiology with environmental science, ecology, evolution, and molecular cell biology. New chapters address Epigenetics, Biomechanics and Locomotion, and Behaviour and Learning. Each chapter contains an extensive bibliography, providing readers with the best sources from the primary literature. Almost three decades after the publication of the first edition, this book remains the only published single-volume work on fish physiology. The fifth edition provides an important reference for new students of fish biology, marine and freshwater biologists, ichthyologists, fisheries scientists, and comparative physiologists.
Download or read book Canadian Journal of Fisheries and Aquatic Sciences written by and published by . This book was released on 2009 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt:
