Download or read book Self assembly of Proteins at Interfaces and Two dimensional Protein Crystallization written by Todd Criswell Edwards and published by . This book was released on 1999 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Material Properties by Design written by Robert Gregory Alberstein and published by . This book was released on 2020 with total page 225 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins, perhaps more than any other class of molecule, make life as we know it possible. The unrivaled champions of functional diversity, the 20+ different amino acids which comprise these polypeptide chains enable them to fold into sequence-specified structures (and structural ensembles) that physically encode their (structure-specified) functions. Yet, the vast majority of cellular proteins operate exclusively as self-assembled multi-subunit complexes, which combinatorially broadens the scope of their (shapes, structures, sizes, and thus) capabilities to encompass roles as molecular-scale catalysts, supramolecular nonequilibrium nanomachines, and cellular-scale motors and intercommunications. This makes proteins highly desirable for the bottom-up construction of new materials: they are perfectly monodisperse nanoscale building blocks, possessing well-defined molecular structure(s) (and thus function(s)) that can be facilely/robustly fine-tuned via routine genetic engineering procedures. As exemplified throughout biology, (synthetic) proteins possess the intrinsic potential to propagate and amplify functional structure (structural function) over multiple scales, promising tailor-made self-assembled macroscale architectures and devices with emergent properties. Towards this goal, this dissertation describes several specific examples of this general principle, whereby new physical phenomena spontaneously manifest within flexible 2D materials self-assembled from an engineered protein. We first report a theoretical and experimental dissection (followed by exploitation) of the bulk-scale behavior of crystalline lattices constructed from a square-shaped tetrameric aldolase with a designed cysteine at its corners (C98RhuA). Upon oxidative self-assembly, the topology and flexibility of the intermolecular disulfide bonds enable the resulting architecture to undergo "breathing" motions that allow it to switch between porous and close-packed conformations without losing crystallinity. We characterized the free energy landscape associated with these dynamic motions, validated its accuracy against experiment, and determined that it is dominated by solvent reorganization entropy. We then exploited the lack of native intermolecular interactions to rationally engineer this landscape through the introduction of negatively charged glutamate residues at prescribed locations, which was then analogously characterized both experimentally and computationally. We further determined that this repulsion could be toggled via the chelation of Ca2+ ions, enabling controllable access to discrete conformational states through environmental conditions. This variant was subsequently utilized as a selective "gatekeeper" coating for a highly specific and sensitive chemical sensor for HCN. Simultaneously, we characterized the self-assembly of C98RhuA at mineral interfaces via in situ AFM. We found that the use of a charged surface as a template for crystallization enabled the formation of three new crystal morphologies. We validated the observed crystal patterns against atomic structural models and used free-energy calculations to rationalize the formation of each crystal morphology. We also determined that C98RhuA possesses a sizeable macrodipole moment (~1200 D), which calculations indicated explained the preference for antiparallel packing of protein units (p4212 symmetry); this is the first time dipole-dipole interactions have been shown to control protein self-assembly. Finally, the alignment of dipole moments in surface-grown crystals was predicted them to be "electrets". Indeed, MD simulations of both p4 symmetry (parallel packing) and p4212 protein crystals revealed that the former exhibits a conformation-dependent membrane voltage of ≤−100 mV (in excellent agreement with analytical calculations), thus delineating the connection between the individual building block and macroscale properties when the patterning of component orientations is controlled. Finally, preliminary computational investigations into the epitaxial growth of C98RhuA crystals on these surfaces are described.
Download or read book Protein Self Assembly written by Jennifer J. McManus and published by Humana. This book was released on 2020-08-08 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.
Download or read book Molecular Self assembly at Interfaces written by Christopher Min-Far Yip and published by . This book was released on 1996 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Kinetics and Thermodynamics of Multistep Nucleation and Self Assembly in Nanoscale Materials Volume 151 written by Gregoire Nicolis and published by John Wiley & Sons. This book was released on 2012-05-22 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Advances in Chemical Physics series—the cutting edge of research in chemical physics The Advances in Chemical Physics series provides the chemical physics and physical chemistry fields with a forum for critical, authoritative evaluations of advances in every area of the discipline. Filled with cutting-edge research reported in a cohesive manner not found elsewhere in the literature, each volume of the Advances in Chemical Physics series presents contributions from internationally renowned chemists and serves as the perfect supplement to any advanced graduate class devoted to the study of chemical physics. This volume explores: Kinetics and thermodynamics of fluctuation-induced transitions in multistable systems (G. Nicolis and C. Nicolis) Dynamical rare event simulation techniques for equilibrium and nonequilibrium systems (Titus S. van Erp) Confocal depolarized dynamic light scattering (M. Potenza, T. Sanvito, V. Degiorgio, and M. Giglio) The two-step mechanism and the solution-crystal spinodal for nucleation of crystals in solution (Peter G. Vekilov) Experimental studies of two-step nucleation during two-dimensional crystallization of colloidal particles with short-range attraction (John R. Savage, Liquan Pei, and Anthony D. Dinsmore) On the role of metastable intermediate states in the homogeneous nucleation of solids from solution (James F. Lutsko) Effects of protein size on the high-concentration/low-concentration phase transition (Patrick Grosfils) Geometric constraints in the self-assembly of mineral dendrites and platelets (John J. Kozak) What can mesoscopic level in situ observations teach us about kinetics and thermodynamics of protein crystallization? (Mike Sleutel, Dominique Maes, and Alexander Van Driessche) The ability of silica to induce biomimetic crystallization of calcium carbonate (Matthias Kellermeier, Emilio Melero-GarcÍa, Werner Kunz, and Juan Manuel GarcÍa-Ruiz)
Download or read book Strategies For Two dimensional Crystallization Of Proteins Using Lipid Monolayers written by Jens Dietrich and published by World Scientific. This book was released on 2005-06-16 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: Strategies for Two-Dimensional Crystallization of Proteins Using Lipid Monolayers presents an overview of different methods that lead to structure determination by electron microscopy. These methods have proven to be extremely successful, especially for elucidating the structure of membrane proteins. Electron crystallography has become an important tool for structure determination of such proteins. This book covers the different practical approaches to two-dimensional crystallization of soluble as well as membrane proteins. From there it takes the reader to equally important issues, such as sample transfer and sample preparation for electron microscopy. In addition, the text provides an introduction to membrane protein structural biology and cryo-electron crystallography, as well as an in-depth discussion on two-dimensional crystallization and surface crystallization./a
Download or read book Molecular Bioelectronics The 19 Years Of Progress Second Edition written by Claudio Nicolini and published by World Scientific. This book was released on 2016-03-29 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular bioelectronics is a field in strong evolution at the frontier of life and materials sciences. The term is utilized in a broad context to emphasize a unique blend of electronics and biotechnology which is seen as the best way to achieve many objectives of industrial and scientific relevance, including biomolecular engineering, bioelectronic devices, materials and sensors capable of optimal hardware efficiency and intelligence and molecular miniaturization.
Download or read book Strategies for Two dimensional Crystallization of Proteins Using Lipid Monolayers written by Jens Dietrich and published by Imperial College Press. This book was released on 2005 with total page 154 pages. Available in PDF, EPUB and Kindle. Book excerpt: Strategies for Two-Dimensional Crystallization of Proteins UsingLipid Monolayers presents an overview of different methods thatlead to structure determination by electron microscopy. These methodshave proven to be extremely successful, especially for elucidating thestructure of membrane proteins.
Download or read book Biopolymers at Interfaces Second Edition written by Martin Malmsten and published by CRC Press. This book was released on 2003-01-15 with total page 930 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition features research from nearly 60 of the profession's most distinguished international authorities. Recognizing emerging developments in biopolymer systems research with fully updated and expanded chapters, the second edition discusses the biopolymer-based multilayer structures and their application in biosensors, the progress made in the understanding of protein behaviour at the air-water interface, experimental findings in ellipsometry and reflectometry, and recent developments concerning protein interfacial behaviour in microfabricated total analysis systems and microarrays. With over 3000 references, this is an essential reference for professionals and students in surface, pharmaceutical, colloid, polymer, and medicinal chemistry; chemical, formulation, and application engineering; and pharmacy.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2009 with total page 840 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Physics of Biomaterials Fluctuations Selfassembly and Evolution written by T. Riste and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent years have seen a growing interest in and activity at the interface between physics and biology, with the realization that both subjects have a great deal to learn from and to teach to one another. A particularly promising aspect of this interface concerns the area of cooperative phenomena and phase transitions. The present book addresses both the structure and motion of biological materials and the increasingly complex behaviour that arises out of interactions in large systems, giving rise to self organization, adaptation, selection and evolution: concepts of interest not only to biology and living systems but also within condensed matter physics. The approach adopted by Physics of Biomaterials: Fluctuations, Self Assembly and Evolution is tutorial, but the book is fully up to date with the latest research. Written at a level appropriate to graduate researchers, preferably with a background either in condensed matter physics or theoretical or physically-oriented experimental biology.
Download or read book Two Dimensional Protein Crystallization at Solid Liquid Interfaces written by Tammy Lynn Calvert and published by . This book was released on 1997 with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Frontiers in Surface Science and Interface Science written by C.B. Duke and published by Gulf Professional Publishing. This book was released on 2002-05-21 with total page 1076 pages. Available in PDF, EPUB and Kindle. Book excerpt: Any notion that surface science is all about semiconductors and coatings is laid to rest by this encyclopedic publication: Bioengineered interfaces in medicine, interstellar dust, DNA computation, conducting polymers, the surfaces of atomic nuclei - all are brought up to date. Frontiers in Surface and Interface Science - a milestone publication deserving a wide readership. It combines a sweeping expert survey of research today with an educated look into the future. It is a future that embraces surface phenomena on scales from the subatomic to the galactic, as well as traditional topics like semiconductor design, catalysis, and surface processing, modeling and characterization. And, great efforts have been made to express sophisticated ideas in an attractive and accessible way. Nanotechnology, surfaces for DNA computation, polymer-based electronics, soft surfaces, interstellar surface chemistry - all feature in this comprehensive collection.
Download or read book Molecular Manufacturing written by C. Nicolini and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present volume is a continuation of the EL.B.A. Forum Series, which was initiated in the spring of 1995 with the first volume, entitled From Neural Network and Biomolecular Engineering to Bioelectronics, in which a brief outline of modem bioelectron ics given as "the use of biological materials and biological architectures for information processing and sensing systems and devices down to molecular level. " The present volume highlights the aspects of advanced biotechnology and electronics originating from molecular manufacturing, which has been emerging as an independent branch of research. This volume appears in a crucial moment, when significant progress has already been made in this strategic field and when technologies derived from it are recognized as critical for the welfare of our society. In addition, acknowledging to the Italian Ministry of University and Scientific and Technological Research for launching the National Research Program "Technologies for Bioelectronics" in 1992 and for continuation of support of this advanced multidisciplinary research, we would like to acknowledge the support of the National Research Council of Italy through the "Molecular Manufacturing" CNR Strategic Project since 1994. The significant unique role of Technobiochip in the organization of the EL.B.A. Forums and in bringing to light the enormous industrial potential of bioelectronics is duly acknowledged, as well as its attraction and support of top level scientists to the series of EL.B.A. Forums of which this volume is part. Dr. Sergey Vakula of the EL.B.A.
Download or read book Design Principle and Application of Self Assembled Nanobiomaterials in Biology and Medicine written by Alok Pandya and published by Academic Press. This book was released on 2022-08-04 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt: Design, Principle and Application of Self-Assembled Nanobiomaterials in Biology and Medicine discusses recent advances in science and technology using nanoscale units that show the novel concept of combining nanotechnology with various research disciplines within both the biomedical and medicine fields. Self-assembly of molecules, macromolecules, and polymers is a fascinating strategy for the construction of various desired nanofabrication in chemistry, biology, and medicine for advanced applications. It has a number of advantages: (1) It is involving atomic-level modification of molecular structure using bond formation advanced techniques of synthetic chemistry. (2) It draws from the enormous wealth of examples in biology for the development of complex, functional structures. (3) It can incorporate biological structures directly as components in the final systems. (4) It requires that the target self-assembled structures be thermodynamically most stable with relatively defect-free and self-healing. In this book, we cover the various emerging self-assembled nanostructured objects including molecular machines, nano-cars molecular rotors, nanoparticles, nanosheets, nanotubes, nanowires, nano-flakes, nano-cubes, nano-disks, nanorings, DNA origami, transmembrane channels, and vesicles. These self-assembled materials are used for sensing, drug delivery, molecular recognition, tissue engineering energy generation, and molecular tuning. Provides a basic understanding of how to design, and implement various self-assembled nanobiomaterials Covers principles implemented in the constructions of novel nanostructured materials Offers many applications of self-assemblies in fluorescent biological labels, drug and gene delivery, bio-detection of pathogens, detection of proteins, probing of DNA structure, tissue engineering, and many more
Download or read book Molecular Assembly in Natural and Engineered Systems written by Stefan Howorka and published by Academic Press. This book was released on 2011-10-26 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores some of the most exciting recent advances in basic research on molecular assembly in natural and engineered systems and how this knowledge is leading to advances in the various fields. This series provides a forum for discussion of new discoveries, approaches, and idea Contributions from leading scholars and industry experts Reference guide for researchers involved in molecular biology and related fields
Download or read book Introduction to Fluorescence Sensing written by Alexander P. Demchenko and published by Springer Science & Business Media. This book was released on 2008-12-21 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence sensing is a rapidly developing field of research and technology. Its target is nearly the whole world of natural and synthetic compounds being detected in different media including living bodies. The application area range from control of industrial processes to environment monitoring and clinical diagnostics. Among different detection methods fluorescence techniques are distinguished by ultimate sensitivity, high temporal and spatial resolution and versatility that allows not only remote detection of different targets but their imaging within the living cells. The basic mechanism of sensing is the transmission of the signal produced by molecular interaction with the target to fluorescent molecules, nanoparticles and nanocomposites with the detection by devices based on modern electronics and optics. In this interdisciplinary field of research and development the book is primarily intended to be a guide for students and young researchers. It is also addressed to professionals involved in active research and product development serving as a reference for the recent achievements. The users of these products will find description of principles that could allow proper selection of sensors for particular needs. Making a strong link between education, research and product development, this book discusses future directions.
