Download or read book Regulation of Cytokinesis in the Budding Yeast Saccharomyces Cerevisiae written by Kay Joanne Simmonite and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Mitotic Exit Network Methods and Protocols written by Fernando Monje-Casas and published by Methods in Molecular Biology. This book was released on 2018-11-23 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book collects the main methodologies used for the analysis of the activity, localization, and regulation of the components of the Mitotic Exit Network (MEN) pathway during mitotic exit in Saccharomyces cerevisiae, as well as for the evaluation of the roles of these proteins in other cellular processes, such as the condensation of the rDNA, the functionality of the mitotic checkpoints, and cytokinesis. Budding yeast serves as an ideal model system for dissecting the mechanisms that regulate cell cycle progression and providing new insights into the molecular basis of cell cycle control and, thus, into the origin of diseases that arise as a consequence of problems during cell division. Therefore, although this volume concentrates on Saccharomyces cerevisiae as a model, it also details the implications that the research about the MEN have on our understanding of the mitotic exit process in higher eukaryotes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Mitotic Exit Network: Methods and Protocols will be a valuable reference for cellular and molecular biologists and biochemists as well as for all scientists interested in the study of the regulation of mitotic exit using budding yeast as a model organism.
Download or read book Systematic Analysis of Cell Size Control in the Budding Yeast Saccharomyces Cerevisiae written by Michael Alexander Cook and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of DNA Replication in Mitosis and Meiosis in the Budding Yeast Saccharomyces Cerevisiae written by Elisabeth Nadjar-Boger and published by . This book was released on 2000 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of the Morphogenesis Checkpoint in the Budding Yeast Saccharomyces Cerevisiae written by Lee Szkotnicki and published by . This book was released on 2008 with total page 228 pages. Available in PDF, EPUB and Kindle. Book excerpt: In response to cytoskeletal perturbations Saccharomyces cerevisiae cells trigger a morphogenesis checkpoint resulting in a G2 arrest. The arrest is due to the Swe1p kinase, which phosphorylates and inhibits the CDK Cdc28p. Dephosphorylation of Cdc28p is catalyzed by the phosphatase Mih1p, and allows for cell cycle progression. Recent work on regulation of the kinase Swe1p has shown a complex pathway of localization specific degradation. Several of the components involved in Swe1p degradation appear to have conserved roles in degradation of the Wee1 family kinases in multicellular systems. Despite this little is known about the steps leading up to the initiation of Swe1p degradation. In this study we have challenged the model that activation of the Swe1p negative regulator, Hsl1p, results from relief of autoinhibition due to septin interaction. Rather, we conclude Hsl1p is subject to phosphorylation of threonine 273, promoted by the septin-associated kinase Elm1p. We identified elm1 mutants in a screen for defects in Swe1p degradation. Additionaly regulation of the morphogenesis checkpoint appears to be acting though regulation of the phophatase Mih1p. In other eukaryotes, Mih1p homologs (Cdc25-family phosphatases) are thought to be regulated by other checkpoints through control of their phosphorylation. We have shown that Mih1p is phosphorylated in response to morphogenesis checkpoint stress. We have also identified a NLS in Mih1p, mutation of which greatly reduces its in vivo activity. These data combined with data from a competing lab suggest that phosphorylation of Mih1p is indeed regulated. We have also investigated the checkpoint response of budded yeast cells. Specifically we have shown that environmental stresses that depolarize the actin cytoskeleton result in a Swe1p mediated G2 delay.
Download or read book The Regulation of the Exit from Mitosis in Saccharomyces Cerevisiae written by Lena H. Hwang and published by . This book was released on 1998 with total page 374 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Multiple Mechanisms Prevent Re replication in Budding Yeast Saccharomyces Cerevisiae written by Van Q. Nguyen and published by . This book was released on 2003 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of Mitotic Exit and Cytokinesis in Saccharomyces Cerevisiae written by Sarah Elizabeth Lee and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell cycle checkpoint control in budding yeast saccharomyces cerevisiae PHD written by Gretchen Louise Kiser and published by . This book was released on 1995 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Molecular and Cellular Biology of the Yeast Saccharomyces Cell cycle and cell biology written by James R. Broach and published by Cold Spring Harbor Laboratory Press. This book was released on 1991 with total page 1148 pages. Available in PDF, EPUB and Kindle. Book excerpt: In all eukaryotic cells, each fundamental processcycle progression and its control, protein secretion and targeting, transcription and its regulation, mRNA processing, and DNA replicationaccomplished by essentially identical cellular machinery composed of essentially identical protein components. This conservation of function has catapulted the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe from parochial backwaters to the forefront of experimental molecular biology: What is true for a yeast is true for an elephant, and in experiments you can get the answer a lot faster from a yeast. This burgeoning appreciation of yeasts as model systems for the study of fundamental cellular processes has highlighted the need for an update of the seminal 1981 monograph The Molecular Biology of the Yeast Saccharomyces. This need is now met by the publication of a three-volume series to serve as the authoritative sequel. The first volume focuses on the genome organization of the yeast Saccharomyces as well as protein translation and its regulation and energy metabolism. Subsequent volumes emphasize such topics as the cell cycle, secretion, and transcription. Together, these volumes provide a comprehensive survey of the molecular and cellular biology of Saccharomyces and Schizosaccharomyces, serving not only as a current summary of every significant area of investigation, but also as a thorough reference source. These volumes are required reading for everyone in the field and anyone curious about the state of the art of molecular and cellular biology.
Download or read book Cell cycle Regulation by Cdk1 in Saccharomyces Cerevisiae written by Jeffrey A. Ubersax and published by . This book was released on 2004 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation and Functions of Cdc14 in Mitotic Exit in Saccharomyces Cerevisiae written by Brett N. Tomson and published by . This book was released on 2008 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: In order to ensure the accurate formation of two daughter cells from one parental cell, the series of events that comprise the mitotic cell division cycle must be carefully regulated. Much of this regulation affects the transitions between the stages of the cell cycle, which are controlled by proteins known as cyclin dependent kinases (CDKs). High levels of CDKs promote mitotic entry, whereas the inactivation of CDKs drives exit from mitosis and entry into the next cell cycle at the G stage. In the budding yeast Saccharomyces cerevisiae, the evolutionarily conserved phosphatase Cdcl4 regulates many events in anaphase, including the inactivation of CDKs. The activity of Cdcl4 is thought to be restricted to anaphase due to two signaling networks, the Cdc fourteen early anaphase release (FEAR) network and the mitotic exit network (MEN). Although the major components of these two pathways have been identified, their regulation is not fully understood. Here we examine how the members of the FEAR network function together to regulate Cdc 14 and its targets in anaphase. One function of Cdc 14 in early anaphase is to promote proper segregation of ribosomal DNA (rDNA). We have determined that Cdcl4 is required to resolve a novel type of linkage between chromosomes, which occurs at the rDNA locus due to transcription-dependent processes. We also characterize the regulation of Cdc 14 activity by the FEAR network component Spo 12. Specifically, we found that Spo 2 must be phosphorylated in a conserved domain during anaphase in order for Cdc 14 to function in early anaphase. Spol2 phosphorylation is dependent on the CDK, Cdc28, whereas Cdcl4 contributes to the dephosphorylation of Spol2. Lastly, we show that Spol2 phosphorylation is regulated by the FEAR network components Espl and Slk19, but not by the MEN. Overall, this thesis describes research that establishes a new model for both the regulation and significance of the FEAR network in controlling Cdc 14 during anaphase in Saccharomyces cerevisiae.
Download or read book Roles of Septins and Type II Myosin in Cytokinesis in the Budding Yeast Saccharomyces Cerevisiae written by Carsten Wloka and published by . This book was released on 2014 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Study on cytokinesis and vesicle trafficking in budding yeast Saccharomyces cerevisiae written by Yulan Xiong and published by . This book was released on 2006 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Regulation of Processing Body Formation in the Budding Yeast Saccharomyces Cerevisiae written by Julie Weidner and published by . This book was released on 2014 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Mitotic Exit Network in Saccharomyces Cerevisiae written by Sue L. Jaspersen and published by . This book was released on 2000 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt:
