Download or read book Purification and Characterization of the Cytochrome O Containgin Terminal Oxidase of Escherichia Coli written by Kimberly Carter and published by . This book was released on 1987 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Purification and Characterization of the Cytochrome O Containing Terminal Oxidase of Escherichia Coli written by and published by . This book was released on 1987 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Purification and Characterization of the Cytochrome D Containing Terminal Oxidase of Escherichia Coli written by Michael Joseph Miller and published by . This book was released on 1984 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Production Purification and Characterization of the Cytochrome Bd Ubiquinol Oxidase from Escherichia Coli and Aquifex Aeolicus written by Jo Hoeser and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isolation of Characterization of New Mutants of the Cytochrome D Terminal Oxidase of Escherichia Coli written by Kristine Leigh Oden and published by . This book was released on 1991 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: The cytochrome d terminal oxidase from Escherichia coli is a two-subunit, three-heme integral membrane cytochrome. Subunit I contains the heme center $bsb{558}$ and Subunit II, is necessary for the binding of the $bsb{595}$ and d heme prosthetic groups. Earlier work suggests that the N-terminus of Subunit I participates in the binding of the $bsb{595}$ and d heme centers. Mutants in the cytochrome d terminal oxidase from Escherichia coli were isolated using hydroxylamine mutagenesis. These mutants, isolated both in the chromosome and on plasmids, were characterized using reduced minus air-oxidized difference spectroscopy, Western immunoblotting, CO difference spectroscopy, and oxidase activity assays. Plasmic-encoded mutations were mapped by marker rescue and identified by DNA sequencing. Two new classes of cytochrome d terminal oxidase mutants were identified. Under aerobic growth conditions, one class produces both subunits, and binds both the $bsb{558}$ and $bsb{595}$ heme centers, but varying amounts of the heme d center. The mutations conferring this phenotype are in the N-terminus of Subunit II. The conclusion is that the N-terminus of Subunit II along with the N-terminus of Subunit I forms the interface between these two subunits where the $bsb{595}$ and d heme centers bind. The second class of mutations also produces both subunits but binds only $bsb{558}$ and $bsb{595}$. No heme d is bound under aerobic conditions. One of these mutations was identified and is also found in the N-terminus of Subunit I confirming its involvement in the formation of the heme-binding interface. There is another phenotype associated with the above mutants. When cells containing these mutations are grown anaerobically, the stability of the mutant enzymes improved as evidenced by increased heme binding and subunit stability. During the course of this work, a technique was developed to create chromosomal replacements in E. coli. Plasmid DNA carrying the desired allele for gene replacement was transformed into a recBC sbcBC mutant of E. coli. A double cross-over event exchanges the wild-type and plasmid-encoded alleles to effect a replacement. This double cross-over event is screened for by the use of a positive drug marker linked to the replacement allele.
Download or read book The purification and characterisation of the cytochrome bd oxidase from Escherichia coli written by Frank Spinner and published by . This book was released on 1992 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Localization of the Prosthetic Group Ligands of Cytochrome O Terminal Oxidase Complex of Escherichia Coli written by Laura Jean Lemieux and published by . This book was released on 1991 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: The cytochrome o ubiquinol oxidase is one of two terminal oxidases present in the aerobic respiratory chain of E. coli. The cytochrome o complex has been purified and found to contain two protoheme IXs and one copper atom. Subsequently the gene encoding the cyo operon has been cloned. The research presented in this thesis focuses on the continued structural analysis of the cytochrome o oxidase complex including the following areas of interest: (i) the DNA sequence of the cyo operon, (ii) subunit analysis, (iii) localization of the prosthetic groups of this complex to specific subunits and (iv) determination of the histidines responsible for ligating the prosthetic groups of this complex. The cyo DNA sequence presented here reveals that there are five open reading frames, cyo A, B, C, D, and E. Similarity searches performed on these putative subunits reveal that three of these ORFs are related to subunits II, I, and III of both bacterial and eukaryotic cytochrome c oxidases. The remaining two putative subunits have sequence similarity with subunits found in bacterial cytochrome c oxidases. Immunological analyses of subclones of cyoA and cyoB demonstrate that they encode subunits II and I, respectively. Subsequent spectroscopic analysis of these subclones and the cyoBCDE subclone localize both the high and low-spin hemes associated with this complex to subunit I (cyoB) of the cytochrome o oxidase. The alignment of subunit I from cytochrome o oxidase with the analogous subunit from over 23 different species of bacterial and eukaryotic aa$sb3$-type cytochrome c oxidases revealed that there are seven conserved histidines referred to as H106, H284, H333, H334, H411, H419 and H421. Physical data on leucine mutations of these histidines indicate that H106 and H421 serve as ligands for the low-spin heme, H333 and H334 are likely to be copper ligands and H284 is probably the high-spin heme ligand.
Download or read book Cytochrome Systems written by S. Papa and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 769 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is based on the proceedings of an International Symposium on "Cytochrome Systems: Molecular Biology and Bioenergetics" that was held at Selva di Fasano near Bari, Italy, between April 7 and 11,1987. It contains papers covering the subjects discussed at the Symposium, contributed both by participants of the meeting and by some invited speakers who were not able to attend. The aim of the Symposium was to bring together experts in various rese arch strategies currently being applied to the study of cytochrome systems, including molecular genetics, protein chemistry, enzymology of electron transfer and protonmotive activity in energy-transducing biological mem branes. Because of the high degree of complexity of cytochrome systems and the increasing sophistication in recent years of the different experimen tal approaches, there has been a growing specialization - sometimes even a tendency to "over-specialization" - among scientists working in this field. This in itself seemed to justify a meeting where representatives of various disciplines could exchange their results and discuss their conclusions. In addition, and perhaps even more importantly, it was felt that meetings of this kind provide an opportunity for a "cross-fertilization" of approaches and ideas among representatives of various fields of science. The present meeting proved to be an ample illustration of the success of such an inter action.
Download or read book Molecular Genetic Studies of the Cytochrome O Terminal Oxidase Complex in Escherichia Coli written by Visala Chepuri and published by . This book was released on 1990 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: The cytochrome $o$ terminal oxidase complex is a component of aerobic respiratory chain of Escherichia coli. This enzyme catalyzes the oxidation of ubiquinol-8 to ubiquinone-8 within the cytoplasmic membrane and the concomitant reduction of O$sb2$ to H$sb2$O. Cytochrome $o$ oxidase has been purified and shown to contain four subunits on SDS-PAGE gels. The cloning of the $cyo$ operon makes it possible to address a number of problems concerning the structure and function of the cytochrome $o$ oxidase complex. Detailed analysis of this operon will enable us to identify the subunit compositions, the locations of the hemes, etc. The thesis research described here has mainly dealt with the structure-function relationship of the cytochrome $o$ oxidase complex. Molecular biological, genetic and some biochemical approaches were used to study the following aspects of cytochrome $o$ oxidase complex: (i) sequence of the $cyo$ operon to determine the primary structure and subunit composition of the cytochrome $o$ oxidase complex, (ii) sequence portions of the polypeptides of cytochrome $o$ complex to correlate with the corresponding ORF's in the $cyo$ operon and to confirm the accuracy of the ORF's deduced by DNA sequence analysis, (iii) use the method of gene fusions to determine the topology of all the subunits of the cytochrome $o$ oxidase complex, (iv) construct a cyo-lacZ fusion and transfer it to the chromosome in order to study regulation in single copy, in this case by the $arc$ system, (v) localize both the cytochromes of the cytochrome $o$ oxidase complex by deletion analysis, and (vi) establish conditions in order to isolate HQNO resistant mutants of the cytochrome $o$ oxidase complex.
Download or read book Analysis of the Topology of the Cytochrome D Terminal Oxidase Complex of Escherichia Coli by Genetic Methods written by Gail Ann Newton and published by . This book was released on 1990 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The cytochrome d terminal oxidase is one of two terminal oxidases in the aerobic respiratory chain of E. coli. The topology of the two subunits of the complex were examined by the use of alkaline phosphatase gene fusions, and models proposed. The expression and assembly of the subunits was also examined. It was found that subunit II of the complex can be expressed independently of subunit I and associates with the cytoplasmic membrane. It does not appear to have any of the cytochrome components associated with it under these conditions. The plasmid expressing subunit II complements another plasmid expressing subunit I, restoring oxidase activity. The incorporation of the $bsb{595}$ and $d$ cytochrome components requires the association of the two subunits. It has also been confirmed that the expression of the cytochrome $d$ complex is not regulated by the presence or absence of heme. A model for the assembly of the complex has been proposed.
Download or read book Analysis of the Topology of the Cytochrome D Terminal Oxidase Complex of E Coli by Genetic Methods written by Gail Ann Newton and published by . This book was released on 1990 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Purification and Identification of a Phospholipid Activated Cytochrome C4 O Terminal Oxidase from Azotobacter Vinelandii written by Tit-Yee Wong and published by . This book was released on 1981 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Molecular Biology of the Cytochrome O Terminal Oxidase of Escherichia Coli written by Chi-tak Doug Au and published by . This book was released on 1987 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Advances in Microbial Physiology written by and published by Academic Press. This book was released on 2015-07-17 with total page 531 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume of Advances in Microbial Physiology continues the long tradition of topical and important reviews in microbiology - Contributions from leading authorities - Informs and updates on all the latest developments in the field
Download or read book Bacterial Energetics written by Terry Krulwich and published by Elsevier. This book was released on 2012-12-02 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacterial Energetics deals with bacterial energetics and the molecular basis of how ions move between and within energy-transducing molecules. Topics covered range from respiration-driven proton pumps and primary sodium pumps to light-driven primary ionic pumps, bacterial transport ATPases, and bacterial photosynthesis. Sodium-coupled cotransport and ion-exchange systems in prokaryotes are also considered. This volume is comprised of 17 chapters and begins with an analysis of the pumps and processes that establish electrochemical ion gradients across bacterial membranes, followed by a discussion on the major types of bioenergetic work that utilize these gradients. The energetics of periplasmic transport systems, chemolithotrophs, methanogens, and protein insertion and translocation into or across membranes are also examined, along with bioenergetics in extreme environments such as high-pressure and high-temperature environments; energetic problems of bacterial fermentations; energetics of bacterial motility; and energetics of the bacterial phosphotransferase system in sugar transport and the regulation of carbon metabolism. This book should be of interest to molecular biologists and biochemists.
Download or read book Corynebacterium glutamicum written by Nami Tatsumi and published by Springer Science & Business Media. This book was released on 2012-08-14 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: Corynebacterium glutamicum was discovered in Japan in 1956 as a natural glutamate producer. Its “microbial factory” qualities, such as its physiological plasticity and robust catalytic functionalities, have since facilitated the development of efficient production processes for amino acids, nucleotides and vitamins. This monograph illustrates how the information gleaned from complete genome sequencing allows the rational engineering of the entire cellular metabolism and how systems biology permits the further optimization of C. glutamicum as a biocatalyst. Aspects of gene regulation, metabolic pathways, sugar uptake, protein secretion, cell division and biorefinery applications highlight the enormous biotechnological and biorefinery potential.
Download or read book Cumulated Index Medicus written by and published by . This book was released on 1996 with total page 1340 pages. Available in PDF, EPUB and Kindle. Book excerpt:
