Download or read book Nonlinear Microscopy for Histology written by Adam Tuer and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nonlinear Microscopy as a Tool for Histological Examinations written by Alexei Kazarine and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "Histological analysis is a fundamental technique in modern medical diagnostics. In this thesis, we present novel imaging techniques based on nonlinear microscopy to provide a most needed update to classical histological methods, some of which were initially developed a hundred years ago. Nonlinear microscopy, a form of advanced laser scanning microscopy, enables three-dimensional imaging within tissue using multiple complementary nonlinear contrast mechanisms including multiphoton fluorescence, second harmonic generation (SHG) and third harmonic generation (THG) each with inherent optical sectioning capabilities. The technique can be applied to both labelled and unlabeled samples, allowing for greater flexibility.This thesis first presents a new histological malaria detection method, third harmonic generation imaging scanning cytometry (THGISC). This technique is based on the combination of THG imaging, high speed motorized scanning and automated software processing. THG is a nonlinear optical process in which three incident photons interact with specific materials to combine into one photon that possesses the sum of their energies resulting in emission that is three times the incident light frequency. Hemozoin, a metabolic byproduct of the malaria parasite, has been shown to present a strong THG signal. We applied THGISC to lab-cultured parasites and malaria infected patient blood samples. We demonstrated that our technique is comparable in effectiveness to the gold standard of malaria detection by manual observation of Giemsa staining while significantly improving throughput thanks to rapid scanning and automated software-based parasitemia determination.A novel multimodal approach for virtual histology of rabbit vocal folds by label-free nonlinear microscopy and nano computed tomography is presented to investigate vocal fold regeneration after injury. Traditionally, vocal fold histology was conducted through mechanical sectioning of tissue into thin slices and conventional histological staining of the slices. Our method enables us to visualize the tissue microarchitecture, particularly extracellular matrix elements such as collagen and elastin, which are critical for the proper functioning of vocal folds. We visualize collagen through SHG and elastin via two photon fluorescence; we are also able to observe other features of interest in whole vocal folds such as muscle, chondrocytes and blood vessels through two photon fluorescence. The resulting images were used to create detailed three-dimensional models of vocal fold tissue that can be computationally cross-sectioned in software for easy visualization. Lastly, we investigate common histological dyes as THG harmonophores, enabling high resolution selective imaging of specific histological tissue features. We first verified the third order nonlinear susceptibility of hematoxylin within hematoxylin and eosin stained human skin and liver tissue sections, demonstrating highly selective nuclear staining by THG. We then identified two new THG harmonophores in common histological stains: Verhoeff-Van-Gieson and picrosirius red. We observe that the iron hematoxylin component of the Verhoeff stain acts as a selective THG harmonophore in tissue, binding to elastic fibers and nuclei. We also showed for the first time a significant nonlinear emission from picrosirius red, enabling highly specific, high contrast collagen detection by THG imaging.The application of nonlinear microscopy to histological investigations provides powerful alternatives to conventional methods. The inherent optical sectioning capability of nonlinear microscopy extends histology into the third dimension while the multimodal approach allows for simultaneous visualization of structures of interest in both unlabeled tissue and through labelling with highly selective harmonophores. We anticipate that these techniques will be adopted in the future of modern biomedical diagnostics." --
Download or read book Nonlinear Microscopy System and Protocol for Rapid Evaluation of Freshly Excised Human Tissue written by Tadayuki Yoshitake and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Histopathology determines disease type/condition by microscopic evaluation of biopsy and surgically excised tissue, playing a critical role in medicine. The clinical protocol for histology requires physical sectioning of tissue, either with intense chemical processing and paraffin embedding or freezing of tissue, limiting the rapid evaluation applications. Nonlinear microscopy (NLM) is an emerging optical sectioning microscopy technology that enables histological visualization of fresh and intact human tissue without requiring physical sectioning.
Download or read book Nonlinear Optical Microscopy for Tissue Imaging and Analysis in Anatomic Pathology written by Kseniya S. Shin and published by . This book was released on 2020 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stimulated Raman Scattering (SRS) microscopy provides chemical information for imaged tissue at submicron resolution. In the experiments described, I combine SRS with other non-linear microscopies, including second harmonic generation (SHG) microscopy and two-photon fluorescence (TPF) microscopy, to develop a methodology for tissue imaging and analysis with anatomic pathology diagnostic challenges in mind. Employing an interdisciplinary approach in collaboration with experts in the field I investigate the diagnostic potential of a multimodal approach to complex tissues such as brain, breast, and bone, illuminating the immense potential non-linear optical microscopy has to offer to pathologists and clinicians as they strive to improve patient care.
Download or read book Polarimetric Second Harmonic Generation Microscopy for Histopathology written by Ahmad Golaraei and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Second-harmonic generation (SHG) is one of the modalities of nonlinear microscopy, which can provide structural visualization and characterization, as well as 3D optical sectioning of the material without the need for staining. Polarimetric SHG microscopy can reveal an immense amount of structural information about the specimen beyond the diffraction limited spatial resolution of the microscope by extracting the second-order nonlinear susceptibility tensor elements ratios. SHG microscopy as well as polarimetric SHG microscopy have become important imaging techniques, specially in the study of biological materials such as collagen. Collagen is the most abundant protein in human body which plays an important role in a number of biological processes including wound repair, tumour development, and cancer metastasis. This thesis focuses on studying the nonlinear optical properties of collagen using polarimetric SHG microscopy and utilizing these nonlinear optical parameters as new metrics for the field of histopathology.Two polarimetric SHG techniques, double Stokes-Mueller polarimetry (DSMP) and reduced DSMP known as linear polarization-in, polarization-out (PIPO) were utilized to study the nonlinear optical properties of collagen fibres in thin tissue sections. Two most commonly assumed symmetry classes for collagen fibres, C6 and C6v were experimentally investigated. C6 class is a more precise description of collagen fibres, as it accounts for chirality of collagen. Measurement of the chiroptical properties of collagen is shown to be linked to the 3D organization of collagen fibres, the polarity of collagen, as well as the relative phase differences between susceptibility tensor elements. Further, PIPO SHG microscopy provides the molecular susceptibility ratios of collagen. Polarimetric SHG microscopy revealed the structural alteration of collagen in tissues with pathological conditions through three main parameters: the susceptibility components ratio, degree of linear polarization of the outgoing SHG and the angle distribution of collagen fibres in the image plane. Polarimetric SHG microscopy shows great potential for understanding the tissue morphology and function, and ultimately as a diagnostic tool in histopathology. The polarimetric SHG technique was further implemented into a large area scanning system for the first time. A large area polarimetric SHG image can elucidate the morphological changes of collagen in diseased tissues such as cancer and can be used as a complementary method to the currently existing techniques in histopathology.
Download or read book Microscopy Techniques written by Jens Rietdorf and published by Springer Science & Business Media. This book was released on 2005-06-23 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: With contributions by numerous experts
Download or read book The Microscope written by Simon Henry Gage and published by . This book was released on 1903 with total page 350 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Biomedical Nonlinear Optical Microscopy written by Barry R. Masters and published by Oxford University Press. This book was released on 2008-05-19 with total page 895 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections. The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing.
Download or read book Handbook of Histology Methods for Bone and Cartilage written by Yuehuei H. An and published by Springer Science & Business Media. This book was released on 2003-05-01 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt: Histotechnology and histomorphometry are the major methodologies in bone and cartila- related research. Handbook of Histology Methods for Bone and Cartilage is an outgrowth of the editors’ own quest for information on bone and cartilage histology and histomorphometry. It is designed to be an experimental guide for personnel who work in the areas of basic and clinical bone and cartilage, orthopedic, or dental research. It is the first inclusive and organized reference book on histological and histomorphometrical techniques on bone and cartilage specimens. The topic has not previously been covered adequately by any existing books in the field. Handbook of Histology Methods for Bone and Cartilage has six major parts and is designed to be concise as well as inclusive, and more practical than theoretical. The text is simple and straightforward. Large numbers of tables, line drawings, and micro- or macro-photographs, are used to help readers better understand the content. Full bibliographies at the end of each chapter guide readers to more detailed information. A book of this length cannot discuss every method for bone and cartilage histology that has been used over the years, but it is hoped that major methods and their applications have been included.
Download or read book The Microscope written by Simon Henry Gage and published by . This book was released on 1911 with total page 408 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Course of Practical Histology written by Sir Edward Albert Sharpey-Schäfer and published by . This book was released on 1877 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Rapid Evaluation of Pathology Using Nonlinear Microscopy with Applications in Breast Cancer Prostate Cancer and Renal Disease written by Lucas C. Cahill and published by . This book was released on 2021 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this thesis, we developed and validated NLM evaluation techniques for breast and prostate cancer and renal disease. The studies in this thesis were performed in close collaboration with the Beth Israel Deaconess Center. We developed a rapid, robust fluorescent staining protocol for NLM imaging with a compact, low cost ytterbium laser and validate it on fresh breast tissue. A randomized controlled trial was designed and initiated to assess the rate of indication for repeat breast surgeries in a study group of patients receiving intraoperative NLM evaluation vs a control group of patients receiving no intraoperative evaluation. We also developed techniques for NLM evaluation of fresh prostatectomy tissue and prostate biopsies and performed blinded readings of NLM images to assess accuracy. An optical clearing and staining technique for rapid three-dimensional analysis of renal biopsies was developed. Overall, this thesis aims to provide a new approach for intraoperative/intraprocedural consultation with NLM, potentially enabling more accurate, rapid, and simplified surgeries/procedures.
Download or read book The Microscopist written by Joseph Henry Wythe and published by . This book was released on 1877 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Microscope and Histology written by Simon Henry Gage and published by . This book was released on 1892 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development and Applications of High Speed and Hyperspectral Nonlinear Microscopy written by Lauren Grosberg and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Nonlinear microscopy refers to a range of laser scanning microscopy techniques that are based on nonlinear optical processes such as two-photon excited fluorescence and second harmonic generation. Nonlinear microscopy techniques are powerful because they enable the visualization of highly scattering biological samples with subcellular resolution. This capability is especially valuable for in vivo and live tissue imaging since it can provide both structural and functional information about tissues in their native environment. With the use of a range of exogenous dyes and intrinsic contrast, in vivo nonlinear microscopy can be used to characterize and measure dynamic processes of tissues in their normal environment. These advances have been particularly relevant in neuroscience, where truly understanding the function of the brain requires that its neural and vascular networks be observed while undisturbed. Despite these advantages, in vivo nonlinear microscopy still faces several major challenges.
Download or read book Second Harmonic Generation Imaging written by Francesco S. Pavone and published by Taylor & Francis. This book was released on 2016-04-19 with total page 465 pages. Available in PDF, EPUB and Kindle. Book excerpt: Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a
Download or read book The Microscope An Introduction to Microscopic Methods and to Histology written by Simon Henry Gage and published by Freeman Press. This book was released on 2011-06 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many of the earliest books, particularly those dating back to the 1900s and before, are now extremely scarce and increasingly expensive. We are republishing these classic works in affordable, high quality, modern editions, using the original text and artwork.
