Download or read book Microfluidics for Investigating Single cell Biodynamics written by Scott Warren Cookson and published by . This book was released on 2008 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: Progress in synthetic biology requires the development of novel techniques for investigating long-term dynamics in single cells. Here, we demonstrate the utility of microfluidics for investigating single-cell biodynamics within tightly-controlled environments in the model organisms Saccharomyces cerevisiae and Escherichia coli. First, we develop a microfluidic chemostat for monitoring single-cell gene expression within large populations of S. cerevisiae over many cellular generations. We overcome typical difficulties in tracking individual cells throughout long sequences of time-lapse microscopy images by constraining colony growth to a monolayer. Second, we construct a variant of this device to elucidate a new mechanism of cellular ordering within dense E. coli populations. By comparing colony growth and alignment inside a long and narrow microfluidic channel with continuum models and discrete element simulations, we conclude that the observed dynamic transition from an isotropic disordered phase to a nematic ordered phase is caused by biomechanical interactions arising from cellular growth and division. Finally, we use microfluidics and time-lapse microscopy to characterize an engineered genetic oscillator in E. coli. We find the circuit to exhibit fast and robust oscillatory periods that can be tuned by altering inducer levels, temperature, and the growth medium. Together, these studies illustrate the role of novel measurement techniques in advancing the goals of synthetic biology. Specifically, the ability to generate single-cell expression profiles for a large number of cells is essential to understanding the roles of regulatory motifs within native and synthetic gene networks. Through such an understanding, we aim to develop an engineering-based approach to building gene-regulatory circuits, where design specifications generated from computational modeling drive the construction of regulatory networks with desired properties.
Download or read book Microfluidics for Single Cell Analysis written by Jin-Ming Lin and published by Springer Nature. This book was released on 2019-08-28 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.
Download or read book Microfluidics in Cell Biology Part B Microfluidics in Single Cells written by and published by Academic Press. This book was released on 2018-08-27 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics in Cell Biology Part B: Microfluidics in Single Cells, Volume 147, a new volume in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various single cell models, including microfludics in micro-organisms, microfluidics for cell culture and cell sorting of mammalian cells, and microfluidics for cell migration. Specific sections in this latest release include Temperature control and drug delivery for cell division cycle control in fission yeast H2O2 stress response in budding yeast, Antibiotic resistance in bacteria, Metabolism in bacteria, Fluidized beds for bacterial sorting and amplification, Microfluidics for cell culture and cell sorting of mammalian cells, Hydrogel microwells, Immune cells migration in complex environments, Neutrophiles migration in health and disease, Cell guidance by physical cues, Stable gradients in gels of extracellular matrix for cancer cell migration, and more. Contains contributions from experts in the field from across the world Covers a wide array of topics on both mitosis and meiosis Includes relevant, analysis based topics
Download or read book Investigating Single Cell Growth Dynamics of Mycrobacteria with Microfluidics written by Solmaz Ariana Golchin and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Microfluidics in Cell Biology Part C Microfluidics for Cellular and Subcellular Analysis written by and published by Academic Press. This book was released on 2018-11-22 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics in Cell Biology Part C, Volume 148, a new release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are three sections on microfluidics in various multi-cellular models, including microfluidics in cell monolayers/spheroids, microfluidics in organ on chips, and microfluidics in model organisms. Specific chapters discuss collective migration in microtubes, leukocyte adhesion dynamics on endothelial monolayers under flow, constrained spheroid for perfusion culture, cells in droplet arrays, heart on chips, kidney on chips, liver on chips, and more. - Contains contributions from experts in the field from across the world - Covers a wide array of topics on both mitosis and meiosis - Includes relevant, analysis based topics
Download or read book Microfluidics for Single cell Analysis written by and published by . This book was released on 2019 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor's laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.
Download or read book Microfluidics for Cells and Other Organisms written by Danny van Noort and published by MDPI. This book was released on 2019-10-23 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics-based devices play an important role in creating realistic microenvironments in which cell cultures can thrive. They can, for example, be used to monitor drug toxicity and perform medical diagnostics, and be in a static-, perfusion- or droplet-based device. They can also be used to study cell-cell, cell-matrix or cell-surface interactions. Cells can be either single cells, 3D cell cultures or co-cultures. Other organisms could include bacteria, zebra fish embryo, C. elegans, to name a few.
Download or read book Microfluidic Systems for Investigating and Modulating Single cell Cancer Invasion Dynamics written by Michael Mak and published by . This book was released on 2014 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: My research entails the design and implementation of microfluidic systems to investigate the mechanical dynamics in cancer invasion at the single -cell level. Metastasis, the process of cancer spreading, is the leading cause of cancer related deaths, but the principles and dynamics that drive this process remain largely unknown. The challenge is in part due to the large spatial and temporal scales that the metastatic process spans, as it could involve single-cells transporting to distal sites across meters and over months to years. To address this challenge, my goal is to create microsystems that aim to recapitulate the critical steps in dissemination. Cell scaled microchannels with subnucleus-scaled barriers are incorporated to elucidate the mechanical transition dynamics of invasive cancer cells. Single-cell - single-barrier interactions are induced and invasive behavior is elicited. Different regulators of invasion are explored, including molecular modulators of microtubules and actomyosin as well as mechanical factors such as dimensional, directional, and other engineered spatially asymmetric cues. Results of my work have shown that microtubule stabilization suppresses cell invasion across subnucleus-scaled barriers, physiologically ubiquitous mechanical cues such as dimensionality and directionality modulate migratory cell decision making, myosin IIa activity inhibition can alter invasion patterns, and certain mechanically asymmetric microenvironments can potentially suppress dissemination via the phenomenon iteratio ad nauseam.
Download or read book Microfluidics Diagnostics written by Valérie Taly and published by Springer Nature. This book was released on with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cell Analysis on Microfluidics written by Jin-Ming Lin and published by Springer. This book was released on 2017-10-25 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a detailed overview of the design, formatting, application, and development of microfluidic chips in the context of cell biology research, enumerating each element involved in microfluidics-based cell analysis, discussing its history, status quo, and future prospects, It also offers an extensive review of the research completed in the past decade, including numerous color figures. The individual chapters are based on the respective authors' studies and experiences, providing tips from the frontline to help researchers overcome bottlenecks in their own work. It highlights a number of cutting-edge techniques, such as 3D cell culture, microfluidic droplet technique, and microfluidic chip-mass spectrometry interfaces, offering a first-hand impression of the latest trends in the field and suggesting new research directions. Serving as both an elementary introduction and advanced guidebook, the book interests and inspires scholars and students who are currently studying microfluidics-based cell analysis methods as well as those who wish to do so.
Download or read book Open Space Microfluidics written by Emmanuel Delamarche and published by John Wiley & Sons. This book was released on 2018-04-30 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: Summarizing the latest trends and the current state of this research field, this up-to-date book discusses in detail techniques to perform localized alterations on surfaces with great flexibility, including microfluidic probes, multifunctional nanopipettes and various surface patterning techniques, such as dip pen nanolithography. These techniques are also put in perspective in terms of applications and how they can be transformative of numerous (bio)chemical processes involving surfaces. The editors are from IBM Zurich, the pioneers and pacesetters in the field at the forefront of research in this new and rapidly expanding area.
Download or read book Microfluidic Devices in Nanotechnology written by Challa S. S. R. Kumar and published by John Wiley & Sons. This book was released on 2010-11-29 with total page 561 pages. Available in PDF, EPUB and Kindle. Book excerpt: Explores the latest applications arising from the intersection of nanotechnology and microfluidics In the past two decades, microfluidics research has seen phenomenal growth, with many new and emerging applications in fields ranging from chemistry, physics, and biology to engineering. With the emergence of nanotechnology, microfluidics is currently undergoing dramatic changes, embracing the rising field of nanofluidics. This volume reviews the latest devices and applications stemming from the merging of nanotechnology with microfludics in such areas as drug discovery, bio-sensing, catalysis, electrophoresis, enzymatic reactions, and nanomaterial synthesis. Each of the ten chapters is written by a leading pioneer at the intersection of nanotechnology and microfluidics. Readers not only learn about new applications, but also discover which futuristic devices and applications are likely to be developed. Topics explored in this volume include: New lab-on-a-chip systems for drug delivery Integration of microfluidics with nanoneuroscience to study the nervous system at the single-cell level Recent applications of nanoparticles within microfluidic channels for electrochemical and optical affinity biosensing Novel microfluidic approaches for the synthesis of nanomaterials Next-generation alternative energy portable power devices References in each chapter guide readers to the primary literature for further investigation of individual topics. Overall, scientists, researchers, engineers, and students will not only gain a new perspective on what has been done, but also the nanotechnology tools they need to develop the next generation of microfluidic devices and applications. Microfluidic Devices for Nanotechnology is a two-volume publication, the first ever to explore the synergies between microfluidics and nanotechnology. The first volume covers fundamental concepts; this second volume examines applications.
Download or read book Microfluidics in Cell Biology Part A Microfluidics for Multicellular Systems written by and published by Academic Press. This book was released on 2018-07-20 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics in Cell Biology Part A: Volume 146, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Unique to this updated volume are sections on Cell monolayers/spheroids, Collective migration in microtubes, Leukocyte adhesion dynamics on endothelial monolayers under flow, Constrained spheroid for perfusion culture, Cells in droplet arrays, Heart on chips, Kidney on chips, Liver on chips and hepatic immune responses, Gut on chips, 3D microvascular model-based lymphoma model, Blood brain barrier on chips, Multi-organ-on-a-chip for pharmacokinetic analysis, Cancer immunotherapy on chips, and more. - Contains contributions from experts in the field from across the globe - Covers a wide array of topics on both mitosis and meiosis - Includes relevant, analysis based topics
Download or read book Microfluidics and Nanotechnology written by Eric Lagally and published by CRC Press. This book was released on 2017-12-19 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: An increasing number of technologies are being used to detect minute quantities of biomolecules and cells. However, it can be difficult to determine which technologies show the most promise for high-sensitivity and low-limit detection in different applications. Microfluidics and Nanotechnology: Biosensing to the Single Molecule Limit details proven approaches for the detection of single cells and even single molecules—approaches employed by the world’s foremost microfluidics and nanotechnology laboratories. While similar books concentrate only on microfluidics or nanotechnology, this book focuses on the combination of soft materials (elastomers and other polymers) with hard materials (semiconductors, metals, and glass) to form integrated detection systems for biological and chemical targets. It explores physical and chemical—as well as contact and noncontact—detection methods, using case studies to demonstrate system capabilities. Presenting a snapshot of the current state of the art, the text: Explains the theory behind different detection techniques, from mechanical resonators for detecting cell density to fiber-optic methods for detecting DNA hybridization, and beyond Examines microfluidic advances, including droplet microfluidics, digital microfluidics for manipulating droplets on the microscale, and more Highlights an array of technologies to allow for a comparison of the fundamental advantages and challenges of each, as well as an appreciation of the power of leveraging scalability and integration to achieve sensitivity at low cost Microfluidics and Nanotechnology: Biosensing to the Single Molecule Limit not only serves as a quick reference for the latest achievements in biochemical detection at the single-cell and single-molecule levels, but also provides researchers with inspiration for further innovation and expansion of the field.
Download or read book Virtual Microfluidics written by Liyi Xu (Ph. D.) and published by . This book was released on 2018 with total page 124 pages. Available in PDF, EPUB and Kindle. Book excerpt: The extensive genomic diversity of complex systems, such as the human gut microbiome and the evolution of human cancer, has been revealed with advances in DNA sequencing. But we are still at an early stage in understanding this genomic diversity to expand our knowledge in biology and for biomedical applications. Taking the diverse human gut microbiome as an example, little is known about the rapid exchange of antibiotic resistance genes and virulence factors as part of the mobile gene flow between the microbes in the gut. Understanding such heterogeneous systems often involves studying the nature and behavior of the individual cells that constitute the system and their interactions. However, it is technically challenging to probe the genomic material of cells, the smallest unit of life and amplify single genomes for sequencing. Current single-cell technologies require complex instrumentation and the data quality is often confounded by biased genome coverage and chimera artifacts. We address these challenges with a new single-cell technology paradigm to make high-quality low-input genomic research accessible to scientists. We developed hydrogel-based virtual microfluidics as a simple and robust platform for the compartmentalization of nucleic acid amplification reactions. We applied whole genome amplification (WGA) to purified DNA molecules, cultured bacterial cells, human gut microbiome samples, and human cell lines in the virtual microfluidics system. We demonstrated whole-genome sequencing of single-cell WGA products with excellent coverage uniformity and markedly reduced chimerism compared with traditional methods. Additionally, we applied single-cell sequencing to identify horizontally transferred genes between the microbes in the gut and revealed human population activities' selective pressure in shaping the mobile gene pools. Altogether, we expect virtual microfluidics will find application as a low-cost digital assay platform and as a high-throughput platform for single-cell sample preparation. This work offers a significant improvement in making high-quality low-input genomic research accessible to scientists in microbiology and oncology.
Download or read book Multidisciplinary Microfluidic and Nanofluidic Lab on a Chip written by Xiujun (James) Li and published by Newnes. This book was released on 2021-09-19 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multidisciplinary Microfluidic and Nanofluidic Lab-on-a-Chip: Principles and Applications provides chemists, biophysicists, engineers, life scientists, biotechnologists, and pharmaceutical scientists with the principles behind the design, manufacture, and testing of life sciences microfluidic systems. This book serves as a reference for technologies and applications in multidisciplinary areas, with an emphasis on quickly developing or new emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology. The book offers practical guidance on how to design, analyze, fabricate, and test microfluidic devices and systems for a wide variety of applications including separations, disease detection, cellular analysis, DNA analysis, proteomics, and drug delivery. Calculations, solved problems, data tables, and design rules are provided to help researchers understand microfluidic basic theory and principles and apply this knowledge to their own unique designs. Recent advances in microfluidics and microsystems for life sciences are impacting chemistry, biophysics, molecular, cell biology, and medicine for applications that include DNA analysis, drug discovery, disease research, and biofluid and environmental monitoring. - Provides calculations, solved problems, data tables and design rules to help understand microfluidic basic theory and principles - Gives an applied understanding of the principles behind the design, manufacture, and testing of microfluidic systems - Emphasizes on quickly developing and emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology
Download or read book Applications of Microfluidic Systems in Biology and Medicine written by Manabu Tokeshi and published by Springer Nature. This book was released on with total page 559 pages. Available in PDF, EPUB and Kindle. Book excerpt:
