Download or read book Investigation on the Chlorophyll a Content of Phytoplankton in the Sea of Azov and the Don River by the Fluorescence Method written by Galina Glushchenko and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Results of in situ fluorescence investigations on chlorophyll-a (chl-a) of phytoplankton in the Sea of Azov (May to June 2018) and in the Don River estuary (September to November 2019) are presented. Continuous data series of chl-a fluorescence were obtained with flow-through fluorometers. Discrete reference measurements of chl-a concentration and phytoplankton biomass were performed by standard methods for the sake of comparison and eventual corrections. The fluorescence intensity values measured in a lateral surface salinity gradient of the Sea of Azov were found correlating with the obtained data on the chl-a concentration (R2 = 0.88, n = 27) and phytoplankton biomass (R2 = 0.90, n = 11). Instead, there was a weak correlation (R2 = 0.40, n = 33) between the fluorescence and phytoplankton biomass found in the estuary. This disparity in correlations was explained by the difference in conditions during measurements, which affected the fluorescence. There were no significant changes in both temperature and coenotic composition of phytoplankton in the course of the marine expedition. The measurements on the river covered a period characterized by the seasonal variations in the phytoplankton composition and by noticeable temperature fluctuations.
Download or read book Fluorescence Methods for Investigation of Living Cells and Microorganisms written by Natalia Grigoryeva and published by BoD – Books on Demand. This book was released on 2020-09 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence methods play a leading role in the investigation of biological objects. They are the only non-destructive methods for investigating living cells and microorganisms in vivo. Using intrinsic and artificial fluorescence methods provides deep insight into mechanisms underlying physiological and biochemical processes. This book covers a wide range of modern methods involved in experimental biology. It illustrates the use of fluorescence microscopy and spectroscopy, confocal laser scanning microscopy, flow cytometry, delayed fluorescence, pulse-amplitude-modulation fluorometry, and fluorescent dye staining protocols. This book provides an overview of practical and theoretical aspects of fluorescence methods and their successful application in the investigation of static and dynamic processes in living cells and microorganisms.
Download or read book Chlorophyll a Fluorescence in Aquatic Sciences Methods and Applications written by David J. Suggett and published by Springer Science & Business Media. This book was released on 2010-08-23 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: Measurements of variable chlorophyll fluorescence have revolutionised global research of photosynthetic bacteria, algae and plants and in turn assessment of the status of aquatic ecosystems, a success that has partly been facilitated by the widespread commercialisation of a suite of chlorophyll fluorometers designed for almost every application in lakes, rivers and oceans. Numerous publications have been produced as researchers and assessors have simultaneously sought to optimise protocols and practices for key organisms or water bodies; however, such parallel efforts have led to difficulties in reconciling processes and patterns across the aquatic sciences. This book follows on from the first international conference on “chlorophyll fluorescence in the aquatic sciences” (AQUAFLUO 2007): to bridge the gaps between the concept, measurement and application of chlorophyll fluorescence through the synthesis and integration of current knowledge from leading researchers and assessors as well as instrument manufacturers.
Download or read book Chlorophyll a Fluorescence in Aquatic Sciences Methods and Applications written by David J. Suggett and published by Springer. This book was released on 2010-09-06 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: Measurements of variable chlorophyll fluorescence have revolutionised global research of photosynthetic bacteria, algae and plants and in turn assessment of the status of aquatic ecosystems, a success that has partly been facilitated by the widespread commercialisation of a suite of chlorophyll fluorometers designed for almost every application in lakes, rivers and oceans. Numerous publications have been produced as researchers and assessors have simultaneously sought to optimise protocols and practices for key organisms or water bodies; however, such parallel efforts have led to difficulties in reconciling processes and patterns across the aquatic sciences. This book follows on from the first international conference on “chlorophyll fluorescence in the aquatic sciences” (AQUAFLUO 2007): to bridge the gaps between the concept, measurement and application of chlorophyll fluorescence through the synthesis and integration of current knowledge from leading researchers and assessors as well as instrument manufacturers.
Download or read book A Critical Comparison of Methods for the Determination of Phytoplankton Chlorophyll written by John T. Salinas and published by . This book was released on 1988 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: The concentration of chlorophyll in natural bodies of water is commonly determined as a means to rapidly estimate the phytoplankton biomass. The literature gives numerous warnings, however, as to the problems involved with accurately determining chlorophyll concentrations. The author's work at Crater Lake, Oregon enticed him to explore critically the spectrometric methods for determining chlorophyll. Four spectrometric methods for the determination of chlorophyll have been investigated. These are the spectrophotometric method, the 'in vitro' fluorometric method, the 'in vivo' fluorometric method and the 'in situ' fluorometric method using fiber optic cables (remote fiber fluorometry). The spectrophotometric trichromatic and monochromatic methods depend on absorption measurements made with a spectrophotometer. The spectral bandpass of the spectrophotometer is a critical variable in the determination of chlorophyll. A spectral bandpass of 2.0 nm has been suggested and shown to be adequate to measure the concentrations of chlorophyll-a. The chlorophyll concentration determined is 15% and 36% too low with spectral bandpasses of 10 and 20 nm, respectively. Increasing the spectrophotometric cell pathlength from 1.0 to 5.0 cm improves the detection limit of the method by a factor of 5. With a 1-cm pathlength cell, the detection limit for chlorophyll-a is 34 [mu]g/L in an extract or 0.34 [mu]g/L in lake water with a concentration factor of 100. Of the fluorometric methods studied, the 'in vitro' uncorrected fluorometric method was shown to be the most precise and to provide the lowest detection limit (4 ng/L in an extract and 0.04 ng/L chlorophyll-a in lake water with a concentration factor of 100). The detection limits for the 'in vivo' and the enhanced 'in vivo' method (using DCMU) fluorometric methods are 5 and 3 ng/L, respectively. The effect of several variables in the sample preparation method for the spectrophotometric and 'in vitro' fluorometric methods were studied with samples of Cronemiller Lake water. No difference in filter retention efficiency at the 95% confidence level was observed when the Millipore HA membrane, S & S glass and Whatman Glass GF/F filters were compared with a solution of titanium dioxide or a natural phytoplankton sample. Following 65 days of storage at 0° C or 238 days of storage at 9° C, the chlorophyll concentration determined did not significantly change from that determined at the beginning of the study. The use of MgCO3 did not change this condition. The 'in vivo' fluorometric technique, applied to water samples from Crater Lake, Oregon, was shown to be influenced by sample temperature and irradiance history. The addition of the herbicide DCMU to a sample has been reported to decrease the dependency of the fluorescence signal on temperature and irradiance history of the sample. This was shown not to be the case. A 10° C decrease of sample temperature resulted in an average 1.8% increase in sample fluorescence. Exposure of a set of samples to solar radiation decreased the fluorescence signal for chlorophyll in the samples. A period of great change in fluorescence signal was followed by an extended period of slower change. After 50 minutes of sample irradiation, the average fluorescence signal decreased over 50% relative to the original signal. A remote fiber fluorometer was constructed to investigate its use for the 'in situ' fluorometric determination of chlorophyll. Transmission characteristics of the fiber showed that light attenuation increased as the wavelength decreased. With a jig that held the excitation and emission fibers at varying distances and angles, it was found that maximum fluorescence signals were recorded as the fiber ends were moved as close as possible to each other and at an angle of about 10°. The 'in situ' detection limit for chlorophyll-a was determined to be 0.64 [mu]g/L using 1-m excitation and emission fibers.
Download or read book Evaluation of Selected Biological Factors that May Have Contributed to the Drought and Post drought Decline in Chlorophyll a Concentration written by Phytoplankton Task Force. Biological Committee and published by . This book was released on 1990 with total page 56 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Cellular Chlorophyll a Fluorescence in Phytoplankton written by Dale Adolph Kiefer and published by . This book was released on 1972 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book New Approaches to the Measurement of Chlorophyll Related Pigments and Productivity in the Sea written by National Aeronautics and Space Administration (NASA) and published by Createspace Independent Publishing Platform. This book was released on 2018-07-18 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the 1984 SBIR Call for Proposals, NASA solicited new methods to measure primary production and chlorophyll in the ocean. Biospherical Instruments Inc. responded to this call with a proposal first to study a variety of approaches to this problem. A second phase of research was then funded to pursue instrumentation to measure the sunlight stimulated naturally occurring fluorescence of chlorophyll in marine phytoplankton. The monitoring of global productivity, global fisheries resources, application of above surface-to-underwater optical communications systems, submarine detection applications, correlation, and calibration of remote sensing systems are but some of the reasons for developing inexpensive sensors to measure chlorophyll and productivity. Normally, productivity measurements are manpower and cost intensive and, with the exception of a very few expensive multiship research experiments, provide no contemporaneous data. We feel that the patented, simple sensors that we have designed will provide a cost effective method for large scale, synoptic, optical measurements in the ocean. This document is the final project report for a NASA sponsored SBIR Phase 2 effort to develop new methods for the measurements of primary production in the ocean. This project has been successfully completed, a U.S. patent was issued covering the methodology and sensors, and the first production run of instrumentation developed under this contract has sold out and been delivered. Booth, C. R. and Keifer, D. A. Unspecified Center CHLOROPHYLLS; FLUORESCENCE; PHYTOPLANKTON; REMOTE SENSING; COST EFFECTIVENESS; FISHERIES; OCEANS; PHOTOSYNTHESIS...
Download or read book Investigation of the Effects of Natural Variations of Phytoplankton on Ocean Color written by Minsu Kim and published by . This book was released on 2001 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Sun induced Fluorescence of Phytoplankton in the Ocean microform Linking Physiology and Remote Sensing written by Yannick Huot and published by Library and Archives Canada = Bibliothèque et Archives Canada. This book was released on 2005 with total page 716 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Remote Determination of Chlorophyll Concentration in the Ocean Using an Optical Pulse Radar written by I. M. Levin and published by . This book was released on 1988 with total page 20 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Study of Fluorescence Based Chlorophyll A Concentration Algorithms for Case 1 and Case 2 Waters written by J. Gower and published by . This book was released on 1999 with total page 39 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Phytoplankton Pigments in Oceanography written by International Council of Scientific Unions. Scientific Committee on Oceanic Research and published by Unesco. This book was released on 1997 with total page 674 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive work stems from the activities of SCOR Working Group 78 (Determination of Photosynthetic Pigments in Seawater) established in 1985. It brings together in one volume all the oceanographer needs to measure and identify pigments as well as to select the most appropriate methods and ensure the best analytical quality control.
Download or read book Spatial and Temporal Variations of Underway in Vivo Chlorophyll Fluorescence from Phytoplankton in the Antarctic Ross Sea written by Joseph Edward Tegeder and published by . This book was released on 2003 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Airborne Laser Fluorosensing of Surface Water Chlorophyll A written by M. Bristow and published by . This book was released on 1979 with total page 84 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Chlorophyll a Concentration of Phytoplankton During the Cruise of the 48th Japanese Antarctic Research Expedition in 2006 2007 written by Japanese Antarctic Research Expedition and published by . This book was released on 2007 with total page 9 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book In Vivo Chlorophyll a Fluorescence in the Vicinity of Warm core Eddies Off the Coast of New South Wales 3 November 1978 written by Commonwealth Scientific and Industrial Research Organization (Australia). Division of Fisheries and Oceanography and published by . This book was released on 1980 with total page 6 pages. Available in PDF, EPUB and Kindle. Book excerpt:
