Download or read book In Vitro Methods in Pharmaceutical Research written by Jose V. Castell and published by Elsevier. This book was released on 1996-10-04 with total page 483 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Vitro Methods in Pharmaceutical Research provides a comprehensive guide to laboratory techniques for evaluating in vitro organ toxicity using cellular models. Step-by-step practical tips on how to perform and interpret assays for drug metabolism and toxicity assessment are provided, along with a comparison of different techniques available. It is a welcome addition to the literature at a time when interest is growing in cellular in vitro models for toxicology and pharmacology studies. Meets the continuing demand for information in this field Compares In Vitro techniques with other methods Describes cell-culture methods used to investigate toxicity in cells derived from different organs Includes contributions by leading experts in the field
Download or read book Monoclonal Antibody Production written by National Research Council and published by National Academies Press. This book was released on 1999-05-06 with total page 74 pages. Available in PDF, EPUB and Kindle. Book excerpt: The American Anti-Vivisection Society (AAVS) petitioned the National Institutes of Health (NIH) on April 23, 1997, to prohibit the use of animals in the production of mAb. On September 18, 1997, NIH declined to prohibit the use of mice in mAb production, stating that "the ascites method of mAb production is scientifically appropriate for some research projects and cannot be replaced." On March 26, 1998, AAVS submitted a second petition, stating that "NIH failed to provide valid scientific reasons for not supporting a proposed ban." The office of the NIH director asked the National Research Council to conduct a study of methods of producing mAb. In response to that request, the Research Council appointed the Committee on Methods of Producing Monoclonal Antibodies, to act on behalf of the Institute for Laboratory Animal Research of the Commission on Life Sciences, to conduct the study. The 11 expert members of the committee had extensive experience in biomedical research, laboratory animal medicine, animal welfare, pain research, and patient advocacy (Appendix B). The committee was asked to determine whether there was a scientific necessity for the mouse ascites method; if so, whether the method caused pain or distress; and, if so, what could be done to minimize the pain or distress. The committee was also asked to comment on available in vitro methods; to suggest what acceptable scientific rationale, if any, there was for using the mouse ascites method; and to identify regulatory requirements for the continued use of the mouse ascites method. The committee held an open data-gathering meeting during which its members summarized data bearing on those questions. A 1-day workshop (Appendix A) was attended by 34 participants, 14 of whom made formal presentations. A second meeting was held to finalize the report. The present report was written on the basis of information in the literature and information presented at the meeting and the workshop.
Download or read book OECD Series on Testing and Assessment Guidance Document on Good In Vitro Method Practices GIVIMP written by OECD and published by OECD Publishing. This book was released on 2018-12-10 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past several decades, there has been a substantial increase in the availability of in vitro test methods for evaluating chemical safety in an international regulatory context. To foster confidence in in vitro alternatives to animal testing, the test methods and conditions under which ...
Download or read book In Vitro Techniques in Research written by John Weston Payne and published by . This book was released on 1989 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Vitro Techniques in Research: Recent Advances This book, based on a recent conference sponsored by the Humane Research Trust and held at the Royal College of Surgeons in London, considers a wide range of the very latest in vitro techniques and their applications. The book will be of interest to all scientists involved in animal experimentation or with pure research into in vitro techniques in biomedical research. Contents In vitro studies with isolated human cells The use of human cell cultures in the development of anti-rheumatic drugs In vitro culture of human articular chondrocytes and intervertebral disc Progress in the study of uptake and transport of exogenous material by nerve cells in tissue culture The acquisition and differentiation of oligodendrocytes in re-aggregating glial cell cultures The use of tumour cell cultures for understanding neuroendocrine cell biology Morphological and biochemical changes in cultured cells exposed to toxic chemicals Testing chemicals for acute toxicity and irritancy: the contribution of in vitro techniques Laboratory methods for assessing carcinogen exposure in man Use of engineered strains of Escherichia coli as an alternative to biological assays for available amino acids in foods and feedstuffs
Download or read book In Vitro Plant Breeding written by Acram Taji and published by CRC Press. This book was released on 2002 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive book presents the basic concepts and applied techniques of plant cell and tissue culture. Covering the history of in vitro breeding as well as emerging research trends, In Vitro Plant Breeding offers specific techniques for crop improvement and breeding. This helpful book is written in clear language illustrated with examples, schematic descriptions, and tables to make the concepts clear. To view an excerpt online, find the book in our QuickSearch catalog at www.HaworthPress.com.
Download or read book Assisted Reproductive Technology Surveillance written by Dmitry M. Kissin and published by Cambridge University Press. This book was released on 2019-07-04 with total page 255 pages. Available in PDF, EPUB and Kindle. Book excerpt: Offers a comprehensive guide to assisted reproductive technology surveillance, describing its history, global variations, and best practices.
Download or read book Dietary Supplements written by National Research Council and published by National Academies Press. This book was released on 2005-01-03 with total page 527 pages. Available in PDF, EPUB and Kindle. Book excerpt: The growing consumer interest in health and fitness has expanded the market for a wide range of products, from yoga mats to the multiple dietary supplements now on the market. Supplements are popular, but are they safe? Many dietary supplements are probably safe when used as recommended. However, since 1994 when Congress decided that they should be regulated as if they were foods, they are assumed to be safe unless the Food and Drug Administration can demonstrate that they pose a significant risk to the consumer. But there are many types of products that qualify as dietary supplements, and the distinctions can become muddled and vague. Manufacturers are not legally required to provide specific information about safety before marketing their products. And the sales of supplements have been steadily increasingâ€"all together, the various types now bring in almost $16 billion per year. Given these confounding factors, what kind of information can the Food and Drug Administration use to effectively regulate dietary supplements? This book provides a framework for evaluating dietary supplement safety and protecting the health of consumers.
Download or read book Individualized In Vitro Fertilization written by Human M. Fatemi and published by Cambridge University Press. This book was released on 2021-03-04 with total page 149 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides updates on the most recent developments in individualized infertility treatment resulting in improved outcomes.
Download or read book Mitochondrial Replacement Techniques written by National Academies of Sciences, Engineering, and Medicine and published by National Academies Press. This book was released on 2016-04-17 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mitochondrial replacement techniques (MRTs) are designed to prevent the transmission of mitochondrial DNA (mtDNA) diseases from mother to child. While MRTs, if effective, could satisfy a desire of women seeking to have a genetically related child without the risk of passing on mtDNA disease, the technique raises significant ethical and social issues. It would create offspring who have genetic material from two women, something never sanctioned in humans, and would create mitochondrial changes that could be heritable (in female offspring), and therefore passed on in perpetuity. The manipulation would be performed on eggs or embryos, would affect every cell of the resulting individual, and once carried out this genetic manipulation is not reversible. Mitochondrial Replacement Techniques considers the implications of manipulating mitochondrial content both in children born to women as a result of participating in these studies and in descendants of any female offspring. This study examines the ethical and social issues related to MRTs, outlines principles that would provide a framework and foundation for oversight of MRTs, and develops recommendations to inform the Food and Drug Administration's consideration of investigational new drug applications.
Download or read book Liquid Culture Systems for in vitro Plant Propagation written by A.K. Hvoslef-Eide and published by Springer Science & Business Media. This book was released on 2005-06-15 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-efficiency micropropagation, with relatively low labour costs, has been demonstrated in this unique book detailing liquid media systems for plant tissue culture. World authorities (e.g. von Arnold, Curtis, Takayama, Ziv) contribute seminal papers together with papers from researchers across Europe that are members of the EU COST Action 843 "Advanced micropropagation systems". First-hand practical applications are detailed for crops – including ornamentals and trees – using a wide range of techniques, from thin-film temporary immersion systems to more traditional aerated bioreactors with many types of explant – shoots to somatic embryos. The accounts are realistic, balanced and provide a contemporary account of this important aspect of mass propagation. This book is essential reading for all those in commercial micropropagation labs, as well as researchers worldwide who are keen to improve propagation techniques and lower economic costs of production. Undergraduate and postgraduate students in the applied plant sciences and horticulture will find the book an enlightened treatise.
Download or read book Drug Absorption Studies written by Carsten Ehrhardt and published by Springer Science & Business Media. This book was released on 2007-12-22 with total page 711 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a well thought-out, highly practical text covering contemporary ‘in vitro’ techniques for drug absorption studies. Starting at the molecular level of investigation, it continues with cell monolayer models (both primary and cell lines) and culminates with in situ techniques as a final testing format. In addition, chapters on high-throughput assays, in vitro-in vivo correlation, bioinformatics and regulatory issues are covered, giving a comprehensive overview of available models and techniques. Moreover, an appendix consisting of a number of practical protocols is available online, updated as needed, and should prove very helpful to apply the techniques directly to the benchside.
Download or read book In Vitro Culture of Mycorrhizas written by Stéphane Declerck and published by Springer Science & Business Media. This book was released on 2005-04-13 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book describing in vitro cultivation of root organs. The text describes various biological aspects such as the physiology, biochemistry, biodiversity, and life cycles of fungi, as well as the effects of symbiosis on plant growth and development, including large-scale fungus production for biotechnological use. Detailed protocols allow the immediate application of the method to culture mycorrhizal fungi in vitro.
Download or read book In Vitro Digestibility in Animal Nutritional Studies written by Pier Giorgio Peiretti and published by MDPI. This book was released on 2020-12-29 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book addresses various aspects of in vitro digestibility: • Application of meta-analyses and machine learning methods to predict methane production; • Methane production of sainfoin and alfalfa; • In vitro evaluation of different dietary methane mitigation strategies; • Rumen methanogenesis, rumen fermentation, and microbial community response; • The role of condensed tannins in the in vitro rumen fermentation kinetics; • Fermentation pattern of several carbohydrate sources; • Additive, synergistic, or antagonistic effects of plant extracts; • In vitro rumen degradation and fermentation characteristics of silage and hay; • In vitro digestibility, in situ degradability, and rumen fermentation of camelina co-products; • Ruminal fermentation parameters and microbial matters to odd- and branched-chain fatty acids; • Comparison of fecal versus rumen inocula for the estimation of NDF digestibility; • Rumen inoculum collected from cows at slaughter or from a continuous fermenter; • Seaweeds as ingredients of ruminant diets; • Rumen in vitro fermentation and in situ degradation kinetics of forage Brassica crops; • In vitro digestibility and rumen degradability of vetch varieties; • Intestinal digestibility in vitro of Vicia sativa varieties; • Ruminal in vitro protein degradation and apparent digestibility of Pisum sativum; • In vitro digestibility studies using equine fecal inoculum; • Effects of gas production recording system and pig fecal inoculum volume on kinetics; • In vitro methods of assessing protein quality for poultry; and • In vitro techniques using the DaisyII incubator.
Download or read book In Vitro Fertilization written by Zsolt Peter Nagy and published by Springer. This book was released on 2019-07-03 with total page 962 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now in its revised and expanded second edition - including over 20 new chapters - this comprehensive textbook remains a unique and accessible description of the current and developing diagnostic and treatment techniques and technologies comprising in vitro fertilization (IVF). Arranged thematically in sections, each chapter covers a key topic in IVF in a sensible presentation. Parts one and two describe the planning, design and organization of an ART unit and IVF laboratory and equipment and systems, respectively. The sections that follow provide detailed descriptions of IVF techniques, embryo culture methods, sperm processing and selection, insemination procedures, micromanipulation, embryo evaluation, cryopreservation, and embryo transfer. Concluding sections address issues of management and regulation of ART labs across the globe, as well as special topics and emerging techniques and devices. Chapter authors, all experts in the field, contribute their expertise from around the world. With the addition of learning key points and review questions at the beginning and end of each chapter, this new edition of In Vitro Fertilization is a readily accessible, high quality instructional resource for reproductive medicine trainees at all levels. Practicing reproductive endocrinologists, urologists, and embryologists also will find value in the book, as will infertility researchers.
Download or read book Bananas and Plantains written by S. Gowen and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 589 pages. Available in PDF, EPUB and Kindle. Book excerpt: In a field of mature bananas, plants can be seen at all stages of vegetative growth and fruit maturity, providing a fascination for anyone who has an interest in growing crops. Banana farmers in the tropics can harvest fruit every day of the year. The absence of seasonality in production is an advantage, in that it provides a continuity of carbohydrate to meet dietary needs as well as a regular source of income, a feature that perhaps has been under-estimated by rural planners and agricultural strategists. The burgeoning interest in bananas in the last 20 years results from the belated realization that Musa is an under-exploited genus, notwithstanding the fact that one genetically narrow group, the Cavendish cultivars, supply a major export commodity second only to citrus in terms of the world fruit trade. International research interest in the diversity of fruit types has been slow to develop, presumably because bananas and plantains have hitherto been regarded as a reliable backyard source of dessert fruit or starch supplying the needs of the household, and in this situation relatively untroubled by pests, diseases or agronomic problems.
Download or read book In Vitro Culture of Higher Plants written by R.L.M Pierik and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Vitro Culture of Higher Plants presents an up-to-date and wide- ranging account of the techniques and applications, and has primarily been written in response to practical problems. Special attention has been paid to the educational aspects. Typical methodological aspects are given in the first part: laboratory set-up, composition and preparation of media, sterilization of media and plant material, isolation and (sub)culture, mechanization, the influence of plant and environmental factors on growth and development, the transfer from test-tube to soil, aids to study. The question of why in vitro culture is practised is covered in the second part: embryo culture, germination of orchid seeds, mericloning of orchids, production of disease-free plants, vegetative propagation, somaclonal variation, test-tube fertilization, haploids, genetic manipulation, other applications in phytopathology and plant breeding, secondary metabolites.
Download or read book Evaluating Chemical and Other Agent Exposures for Reproductive and Developmental Toxicity written by National Research Council and published by National Academies Press. This book was released on 2001-04-13 with total page 261 pages. Available in PDF, EPUB and Kindle. Book excerpt: The United States Navy has been concerned for some time with protecting its military and civilian personnel from reproductive and developmental hazards in the workplace. As part of its efforts to reduce or eliminate exposure of Naval personnel and their families to reproductive and developmental toxicants, the Navy requested that the National Research Council (NRC) recommend an approach that can be used to evaluate chemicals and physical agents for their potential to cause reproductive and developmental toxicity. The NRC assigned this project to the Committee on Toxicology, which convened the Subcommittee on Reproductive and Developmental Toxicology, to prepare this report. In this report, the subcommittee recommends an approach for evaluating agents for potential reproductive and developmental toxicity and demonstrates how that approach can be used by the Navy. This report has been reviewed in draft form by individuals chosen for their diverse perspectives and technical expertise, in accordance with procedures approved by the NRC's Report Review Committee. The purpose of this independent review is to provide candid and critical comments that will assist the institution in making its published report as sound as possible and to ensure that the report meets institutional standards for objectivity, evidence, and responsiveness to the study charge. The review comments and draft manuscript remain confidential to protect the integrity of the deliberative process. We wish to thank the following individuals for their review of this report: James Chen (National Center for Toxicological Research), George Daston (Procter and Gamble Company), Jerry Heindel (National Institute of Environmental Health Sciences), Grace Lemasters (University of Cincinnati), and John Young (National Center for Toxicological Research).
