Download or read book Impact of Industrial Biocides on Bacterial Isolates from Hydraulic Fracturing Produced Water written by and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract : Biocides are antimicrobial compounds that are designed to kill diverse groups of microbes in an untargeted fashion. Glutaraldehyde and DBNPA are commonly used to manage microbial growth in different industries. It is possible that biocide use may result in the development of bacterial resistance. However, resistance to DBNPA is believed to be limited under normal use conditions. We isolated a number of bacteria from produced water from a hydraulically fractured site in West Texas. We then tested the ability of these isolates to resist Glutaraldehyde and DBNPA. In this study, we found that these isolates have varying resistance to these biocides. Importantly we show that some of these isolates are resistant to DBNPA. Isolates had higher minimum inhibitory concentrations (MICs) for glutaraldehyde after twenty-four hour incubation than after one hour. The opposite was seen with DBNPA, where cells that survived one hour of treatment were killed after the twenty-four hour incubation. Previous work has shown that many bacteria that are resistant to biocides can also be resistant to antibiotics. We determined the minimum inhibitory concentration (MIC) of Tetracycline, Ampicillin and Streptomycin. We found that the resistance profiles for these isolates varied. There was little relationship between the bacteria resistant to biocide and antibiotics. To determine the genetic mechanism for biocide and antibiotic resistance we sequenced the genomes of several of these isolates. Genomes were sequenced using Illumina sequencing. Raw reads were Quality filtered, assembled using SPAdes, and annotated using RAST. Antibiotic Resistance genes (ARGs) were identified for each of these isolates using the RGI from the CARD database. The number of ARGs varied from eight in some organisms to zero in others. There was no relationship between the number of genes and high antibiotic resistance. Our results suggest a complex relationship between the presence of antibiotic resistance genes and the antimicrobial resistance profiles. More work is needed to understand the prevalence and genetic basis for biocide resistance

Download or read book Environmental Impacts of Biocides Used in Hydraulic Fracturing written by Maria Fernanda Campa Ayala and published by . This book was released on 2018 with total page 177 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goal of this dissertation was to better understand the environmental impacts of biocides used in unconventional oil and gas (UOG) practices. Specifically focusing on how industrial biocides may impact aquatic microbial communities, biocide degradation potential, and contribute to antimicrobial resistance propagation. Recently, the energy sector has seen a stark increase in biocide use, due to the dramatic growth in hydraulic fracturing (HF) operations. Biocides in HF are used to suppress microbial-induced corrosion, biofouling, and hydrogen sulfide production. The implications of biocide usage expansion, its impacts to antimicrobial resistance, and to environmental and public health risks are not fully understood. To understand these knowledge gaps, microcosm-based studies were used to investigate the effect of biocide addition to both HF-impacted and unimpacted streams. The two most common HF biocides, glutaraldehyde and 2-2-dibromo-3-nitrilopropionamide (DBNPA), were used in two otherwise identical experiments. Degradation of the biocides and microbial community changes were measured over time. Results suggest that glutaraldehyde is more persistent in stream waters previously impacted by HF. However, the microbial community was able to tolerate it as shown by higher microbial diversity and biomass. The DBNPA microcosms experiment showed that previous HF impact, associated with higher total organic carbon, favors a less toxic and persistent DBNPA degradation pathway. Many unidentified brominated species were detected in both HF-impacted and unimpacted conditions. Whole genome sequencing of strains belonging to environmentally relevant genera enriched during the biocide microcosm and isolated in biocide plates were investigated to find relevant genes correlated with DBNPA resistance. Thirteen orthologous genes with predicted functions such as mobile elements (recombinase and terminase), efflux pumps, and possible enzymatic deactivation of the biocide were found. Finally, the knowledge gaps that need to be addressed to perform a risk assessment of antimicrobial resistance caused by biocide usage in UOG production were identified and discussed. This work should help oil and gas operators, environmental response teams, and regulators reach convergence about the risks and aquatic microbial community response to biocides in UOG production and help with preventive strategies and better formulations to minimize the effect this practice has in the environment.

Download or read book BIOCIDE RESISTANCE AS A RESULT OF EXPOSURE TO BIOCIDES USED IN HYDRAULIC FRACTURING written by and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract : Microbial resistance to antimicrobials is an important topic to investigate not only for our health but for the environment. There is a growing concern about expanding microbial resistance to both antibiotics and other antimicrobials such as biocides. We wanted to know how bacteria previously exposed to hydraulic fracturing fluids, including biocides, would react to biocide exposure and what the mechanism of resistance looked like. In order to test this hypothesis bacterial isolates were obtained from water and sediment from a stream that had been previously exposed to a spill of hydraulic fracturing water. Thirty bacterial isolates were obtained from these samples that could withstand a moderate dose of two biocides (Glutaraldehyde and 2,2-dibromo-3-nitrilopropionamide (DBNPA)). These isolates then had their DNA extracted and taxonomy identified using 16S rRNA sequencing. We also tested minimum inhibitory concentrations (MIC) for each isolate against each biocide. This data however was not conclusive. Attempts were made to perform whole genome sequencing on these isolates to better understand resistance mechanisms. Sequencing was also attempted but due to complications was not completed. To increase our understanding of the mechanism of resistance a comparative genomic study was undertaken to compare between hydraulic fracturing associate Bacillus spp. and non-hydraulic fracturing associated relatives. Overall, isolates resistant to glutaraldehyde, DBNPA or both were obtained and identified. More isolates were found resistant to either glutaraldehyde or DBNPA not both. Meaning that the mechanisms of resistance may be different for the two biocides.

Download or read book Modern Shale Gas Development in the United States written by and published by . This book was released on 2009 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Petroleum Microbial Biotechnology Challenges and Prospects written by Wael A. Ismail and published by Frontiers Media SA. This book was released on 2017-09-08 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: Petroleum hydrocarbons are both a product of, and rich substrate for, microorganisms from across all Domains of life. Rooted deeply in the history of microbiology, hydrocarbons have been studied as sources of carbon and energy for microorganisms for over a century. As global demand for petroleum and its refined products continues to rise, so do challenges associated with environmental pollution, oil well souring, infrastructure corrosion, oil recovery, transport, refining, and upgrading of heavy crude oils and bitumens. Advances in genomics, synthetic biology and metabolic engineering has invigorated interest in petroleum microbial biotechnology as interest grows in technologies for in situ methane production, biodesulfurization and biodenitrogenation, bio-upgrading of heavy crudes, microbial enhanced oil recovery, corrosion control, and biocatalysts for generating value-added products. Given the complexity of the global petroleum industry and the harsh conditions in which it operates, a deeper understanding of the ecophysiology of aerobic and anaerobic microbial communities that have associations with petroleum hydrocarbons is needed if robust technologies are to be deployed successfully. This research topic highlights recent advances in microbial enhanced oil recovery, methanogenic hydrocarbon metabolism and carbon dioxide sequestration, bioremediation, microbiologically influenced corrosion, biodesulfurization, and the application of metagenomics to better understand microbial communities associated with petroleum hydrocarbons.

Download or read book Aquatic Microbial Communities written by John Cairns and published by . This book was released on 1977 with total page 720 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Microbiologically Influenced Corrosion in the Upstream Oil and Gas Industry written by Torben Lund Skovhus and published by CRC Press. This book was released on 2017-03-03 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microorganisms are ubiquitously present in petroleum reservoirs and the facilities that produce them. Pipelines, vessels, and other equipment used in upstream oil and gas operations provide a vast and predominantly anoxic environment for microorganisms to thrive. The biggest technical challenge resulting from microbial activity in these engineered environments is the impact on materials integrity. Oilfield microorganisms can affect materials integrity profoundly through a multitude of elusive (bio)chemical mechanisms, collectively referred to as microbiologically influenced corrosion (MIC). MIC is estimated to account for 20 to 30% of all corrosion-related costs in the oil and gas industry. This book is intended as a comprehensive reference for integrity engineers, production chemists, oilfield microbiologists, and scientists working in the field of petroleum microbiology or corrosion. Exhaustively researched by leaders from both industry and academia, this book discusses the latest technological and scientific advances as well as relevant case studies to convey to readers an understanding of MIC and its effective management.

Download or read book Oilfield Microbiology written by Torben Lund Skovhus and published by CRC Press. This book was released on 2019-03-29 with total page 308 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microorganisms can be both beneficial and harmful to the oil and gas industry and therefore there is an increasing need for the oil industry to characterize, quantify and monitor microbial communities in real time. Oilfield Microbiology offers a fundamental insight into how molecular microbiological methods have enabled researchers in the field to analyze and quantify in situ microbial communities and their activities in response to changing environmental conditions. Such information is fundamental to the oil industry to employ more directed, cost-effective strategies to prevent the major problems associated with deleterious microbial activities (e.g., souring and biocorrosion), as well as to encourage beneficial microbe activity (e.g. oil bioremediation). The aim of the book is to understand how the technological advances in molecular microbiological methods over the last two decades are now being utilized by the oil industry to address the key issues faced by the sector. This book contains a comprehensive collection of chapters written by invited experts in the field from academia and industry and provides a solid foundation of the importance of microbes to the oil and gas industry. It is aimed at microbial ecologists, molecular biologists, operators, engineers, chemists, and academics involved in the sector.

Download or read book Biogeography of Microscopic Organisms written by Diego Fontaneto and published by Cambridge University Press. This book was released on 2011-05-19 with total page 387 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bringing together the viewpoints of leading experts in taxonomy, ecology and biogeography of different taxa, this book synthesises discussion surrounding the so-called 'everything is everywhere' hypothesis. It addresses the processes that generate spatial patterns of diversity and biogeography in organisms that can potentially be cosmopolitan. The contributors discuss questions such as: are microorganisms (e.g. prokaryotes, protists, algae, yeast and microscopic fungi, plants and animals) really cosmopolitan in their distribution? What are the biological properties that allow such potential distribution? Are there processes that would limit their distribution? Are microorganisms intrinsically different from macroscopic ones? What can microorganisms tell us about the generalities of biogeography? Can they be used for experimental biogeography? Written for graduate students and academic researchers, the book promotes a more complete understanding of the spatial patterns and the general processes in biogeography.

Download or read book An Independent Scientific Assessment of Well Stimulation in California written by Ccst and published by . This book was released on 2015-01-01 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Soil pollution a hidden reality written by Food and Agriculture Organization of the United Nations and published by Food & Agriculture Org.. This book was released on 2018-04-30 with total page 156 pages. Available in PDF, EPUB and Kindle. Book excerpt: This document presents key messages and the state-of-the-art of soil pollution, its implications on food safety and human health. It aims to set the basis for further discussion during the forthcoming Global Symposium on Soil Pollution (GSOP18), to be held at FAO HQ from May 2nd to 4th 2018. The publication has been reviewed by the Intergovernmental Technical Panel on Soil (ITPS) and contributing authors. It addresses scientific evidences on soil pollution and highlights the need to assess the extent of soil pollution globally in order to achieve food safety and sustainable development. This is linked to FAO’s strategic objectives, especially SO1, SO2, SO4 and SO5 because of the crucial role of soils to ensure effective nutrient cycling to produce nutritious and safe food, reduce atmospheric CO2 and N2O concentrations and thus mitigate climate change, develop sustainable soil management practices that enhance agricultural resilience to extreme climate events by reducing soil degradation processes. This document will be a reference material for those interested in learning more about sources and effects of soil pollution.

Download or read book Petroleum Microbiology written by Bernard Ollivier and published by . This book was released on 2005 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: A state-of-the-art presentation of the specific microbes that inhabit oil reservoirs, with an emphasis on the ecological significance of anaerobic microorganisms. Provides fundamental and applied biological approaches, and serves as an invaluable reference source for petroleum engineers, remediation professionals, and field researchers."

Download or read book Trends in Oil and Gas Corrosion Research and Technologies written by A. M. El-Sherik and published by Woodhead Publishing. This book was released on 2017-06-09 with total page 928 pages. Available in PDF, EPUB and Kindle. Book excerpt: Trends in Oil and Gas Corrosion Research and Technologies: Production and Transmission delivers the most up-to-date and highly multidisciplinary reference available to identify emerging developments, fundamental mechanisms and the technologies necessary in one unified source. Starting with a brief explanation on corrosion management that also addresses today's most challenging issues for oil and gas production and transmission operations, the book dives into the latest advances in microbiology-influenced corrosion and other corrosion threats, such as stress corrosion cracking and hydrogen damage just to name a few. In addition, it covers testing and monitoring techniques, such as molecular microbiology and online monitoring for surface and subsurface facilities, mitigation tools, including coatings, nano-packaged biocides, modeling and prediction, cathodic protection and new steels and non-metallics. Rounding out with an extensive glossary and list of abbreviations, the book equips upstream and midstream corrosion professionals in the oil and gas industry with the most advanced collection of topics and solutions to responsibly help solve today's oil and gas corrosion challenges. - Covers the latest in corrosion mitigation techniques, such as corrosion inhibitors, biocides, non-metallics, coatings, and modeling and prediction - Solves knowledge gaps with the most current technology and discoveries on specific corrosion mechanisms, highlighting where future research and industry efforts should be concentrated - Achieves practical and balanced understanding with a full spectrum of subjects presented from multiple academic and world-renowned contributors in the industry

Download or read book Handbook of Biocide and Preservative Use written by H.W. Rossmoore and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt: My professional interest in antimicrobial agents and contamination control goes back 50 years to my tour as a microbiologist in a field hospital in Europe during World War II. With no experience and relying solely on a military handbook, I prepared thermometer trays with jars of blue bichloride of mercury and pink isopropyl alcohol. A preliminary typhoid diagnosis of one of our cooks resulted in the need for lab testing. His stool specimen and its subsequent disposal was my problem. My handbook said bum it. So burn it T did, in a five-gallon can with gasoline. Flames shot up almost six feet, and my next mistake was to extinguish them with carbon tetrachloride. This resulted in the production of lethal phosgene gas. The hospital had a near disaster. I could say that at that moment I vowed to write a how-to book so that such stupidities could be avoided. Nevertheless, when I was offered the opportunity to edit this book I thought back on the need for a real, practical treatment of my subject. This book, then, is a practical handbook for technical service personnel and scientists who are not necessarily specialists in microbiology. It provides information on suitable antimicrobial agents appropriate to their particular problem-solving needs and information on the microbial groups contributing to the specific problem, their ecologies, and strategies for controlling their access to the area or material of interest.

Download or read book Oil and Gas Production Handbook An Introduction to Oil and Gas Production written by Havard Devold and published by Lulu.com. This book was released on 2013 with total page 84 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Applied Microbiology and Molecular Biology in Oilfield Systems written by Corinne Whitby and published by Springer Science & Business Media. This book was released on 2010-10-13 with total page 273 pages. Available in PDF, EPUB and Kindle. Book excerpt: Applied Microbiology and Molecular Biology in Oil Field Systems addresses the major problems microbes cause in oil fields, (e.g. biocorrosion and souring) and how beneficial microbial activities may be exploited (e.g. MEOR and biofuels). The book describes theoretical and practical approaches to specific Molecular Microbiological Methods (MMM), and is written by leading authorities in the field from both academia and industry. The book describes how MMM can be applied to faciliate better management of oil reservoirs and downstream processes. The book is innovative in that it utilises real industrial case studies which gives useful technical and scientific information to researchers, engineers and microbiologists working with oil, gas and petroleum systems.

Download or read book Shale Oil and Gas written by Vikram Rao and published by RTI Press. This book was released on 2015-08-09 with total page 184 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Promise and the Peril