Download or read book Immunoenzymatic Techniques written by S. Avrameas and published by Elsevier Science & Technology. This book was released on 1983 with total page 428 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aandacht voor de enzymatische-immunocytochemie met de nadruk op intracellulaire penetratie van gelabelde antilichamen en de toepassing van "double staining"-methodes of monoclonale antilichamen in normale en in diverse experimentele of pathologische omstandigheden; kwantitatieve aspecten van immuno-enzymatische technieken, zoals nieuwe technische vindingen en toepassingen op verschillende gebieden, bijvoorbeeld biologie, humane en veterinaire geneeskunde, fytopathologie en experimentele ziekten
Download or read book Immunoenzymatic Assay Techniques written by R. Malvano and published by Springer. This book was released on 1980 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book ELISA written by John R. Crowther and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt: ELISA: Theory and Practice introduces to scientists at all levels of expertise the principles of the most commonly used assay technique known as the Enzyme Linked Immunosorbent Assay. The book provides readers with full descriptions of the basic systems that make ELISA one of the most powerful techniques in science today, and also examines in detail the data obtained by ELISA and their analysis and actual manipulation. ELISA: Theory and Practice is designed not only to train novices in the science of ELISA, but also to aid investigators experienced in any of the biological sciences in performing independently assays of antibodies and antigens. Mastery of the book's contents will allow readers to fully appreciate exactly how and why assays function, as well as permit the efficient development of individual assays that are both rapid and accurate.
Download or read book Cell Growth written by Claudio Nicolini and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 806 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Nonisotopic Immunoassay written by That T. Ngo and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: The basis of all immunoassays is the interaction of antibodies with antigens. The most widely used immunoassay technique is radioimmunoassay (RIA) which was first developed by Yalow and Berson in 1959. The principle of RIA is elegantly simple. It utilizes a competitve binding reaction between analytes and a radio-labeled analog of the analytes (the tracer) for anti-analyte antibodies. In addition to its exquisite specificity, extraordinary sensitivity, good accuracy and precision, ease and rapidity of assay and simplicity of assay development, the applicability of RIA to a wide variety of substances has made it one of the most powerful and versatile analytical methods of the 20th century and beyond. Millions of RIA's are being performed annually on clinical, biological and environmental samples in licensed laboratories. In order to expand the use of RIA beyond the confines of these laboratories to areas like physician's offices, patients' homes, economically less developed countries, agricultural fields, large scale and continuing screening tests for infectious diseases, it has become necessary to develop non-isotopic labels. Indeed the last fifteen years have seen the development of a great number of ingenious non-isotopic labels in immunoassay so that a whole new industry capitalizing on the potential market for non isotopic immunoassays has appeared. It is the purpose of this volume to present in depth, state-of-the-art reviews on techniques used in non-isotopic immunoassays. Topics covered include: (1) Enzyme-labeled immunoassay; (2) Luminescene immunoassay; (3) Immunoassay at liquid-solid interface; (4) Membrane immunoassay and (5) "Particle"-mediated immunoassay.
Download or read book Immunoassay written by Brian Law and published by CRC Press. This book was released on 2002-09-11 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunoassay development is a multidisciplinary activity involving a wide range of skills possessed by few laboratories. This presentation of tried and tested methods should enable scientists and researchers in the pharmaceutical and related industries to more rapidly and effectively develop immunoassays upon which their work is becoming heavily dependent.; Important methods are included for preparing Lapten-protein conjugates and raising the necessary antibodies, concentrating on polyclonal sera, as well as methods for the synthesis of radio and enzyme labelled tracers. Particular attention is paid to the requirements of the regulatory authorities such as the FDA (Food and Drug Administration) with respect to assay validation. Further chapters deal with assay development and optimization, curve fitting and quality control procedures.
Download or read book Immunoenzymatic Techniques written by Thérèse Ternynck and published by Elsevier Publishing Company. This book was released on 1990 with total page 160 pages. Available in PDF, EPUB and Kindle. Book excerpt: This practical manual is translated from the highly successful French publication Techniques Immunoenzymatiques', published by INSERM for the Societe d'Immunologie. A useful bench-tool describing a series of experimental procedures for the application of immunoenzymatic methods and the preparation of reagents employed. Attractive features include: - clear, concise descriptions of underlying principles - extensive use of technical notes to facilitate application
Download or read book Immunoenzymatic Techniques written by International Symposium on Immunoenzymatic Techniques and published by . This book was released on 1983 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Evidence Based Medical Monitoring written by Paul P. Glasziou and published by John Wiley & Sons. This book was released on 2008-04-30 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: Monitoring is a major component of management of chronic diseases such as diabetes, cardiovascular disease, arthritis and depression. Yet poor monitoring means healthcare costs are rising. This book discusses how monitoring principles adopted in other spheres such as clinical pharmacology and evidence-based medicine can be applied to chronic disease in the global setting. With contributions from leading experts in evidence-based medicine, it is a ground-breaking text for all involved in delivery of better and more effective management of chronic illnesses.
Download or read book Handbook of Immunochemistry written by Miroslav Ferencik and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book appeared in two previous Slovak editions for university students in Czechoslovakia. This edition presents a completely new version updated according to recent advances not only in immunochemistry and essential immunology but also in molecular biology, biochemistry and molecular genetics. The scope of the book is considerable since the goal was to cover the field of immunochemistry from the widest point of view including both the topic and methods of contemporary immunochemistry. Each chapter provides basic information on a specific subtopic, clearly and understandab ly, and presents principles of individual immunochemical methods. I am confident that the book will fill the gap between the books on essential immunology and highly specialised books on individual areas of immunoche mistry (e. g. on antibodies, antigens, numerous immunochemical techniques, etc. ). It may also prove useful for beginning investigators from different biological and medical fields as it supplies basic information needed for solving their scientific problems by immunochemical approaches. I do hope that readers will find the text stimulatory and pleasury to read. I wish to thank all colleagues and friends for supplying their own results, suggestions and for their encouraging comments. My thanks go also to the editors and publishers for their valuable contribution to the preparation of the book. 1 Introduction The term immunochemistry was coined by the Swedish chemist ARRHENIUS who used it for the first time in his lectures in 1907.
Download or read book Principles and Practice of Immunoassay written by Christopher P. Price and published by . This book was released on 1997 with total page 688 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aims of this new edition are the same as the first edition, ie: to link the fundamental aspects of immunoassay together with the principles of the major techniques in use today. The highly acclaimed first edition was recommended as 'an excellent comprehensive source of background knowledge ... and a constant source of reference'. This new edition again covers the basic principles before going on to describe all the latest developments in this rapidly developing field. Completely new chapters such as Alternative Binding Particles, Microfabricated optical, and Microfabricated electrochemical immunoassay, together with the complete updating of all the techniques bring the reader/researcher up to date with all immunoassays. Upwards of a dozen or so contributors, expert in their field, have contributed to individual chapters. Finally the editors consider what the prospects are for immunoassay techniques in the future.
Download or read book ELISA written by Robert Hnasko and published by Humana. This book was released on 2015-07-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and quality assurance processes in food and beverage production. An introductory chapter orients the reader on the basic structure and function of immunoglobulins and their fragments while subsequent chapters outline the methodology to generate monoclonal antibodies using hybridoma technology and the general methods used to purify antibodies. Multiple chapters demonstrate how to creatively use the properties of the antibody to identify, localize and quantify target analytes to answer questions and resolve problems. The reader will learn how to use a variety of immunoassay strategies, reporters and detection systems that will undoubtedly facilitate their efforts to gain answers to their own questions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, ELISA: Methods and Protocols seeks to provide both professionals and novices with the technical information necessary for the reader to successfully use the immunoassay as part of the discovery process.
Download or read book Immunoenzymatic Techniques written by Gérard Feldmann and published by North-Holland. This book was released on 1976 with total page 552 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzyme linked Immunosorbent Assay ELISA written by Samira Hosseini and published by Springer. This book was released on 2017-12-30 with total page 115 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.
Download or read book Handbook of ELISPOT written by Alexander E. Kalyuzhny and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this first book dedicated entirely to the ELISPOT, a critical enzyme-linked immunospot assay used widely in biomedical research, recognized experts with first-hand experience detail how to design, perform, and analyze these assays. The readily reproducible techniques they provide cover a wide variety of topics, including the use of membrane-backed plates, the standardization and validation procedures, the removal of cells from ELISPOT plates, cell separation techniques, and the quantification of ELISPOT data. There are also numerous ELISPOT applications involving animal models, human cells, measles, multiple sclerosis, immune responses, multicytokine detection systems, and immunocytochemistry. Highlights include dual-color and multiplex ELISPOT assays, use of the ELISPOT assay on feline lymphocytes, standardization of the ELISPOT procedure, and combining the ELISPOT assay with immunohistochemistry.
Download or read book Encyclopedic Reference of Immunotoxicology written by Hans-Werner Vohr and published by Springer. This book was released on 2005-06-01 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work provides rapid access to focused information on topics of Immunotoxicology not only for scientists and those dealing with laboratory aspects but also for lecturers and advanced students. Over 200 contributing authors – including many of the world’s top specialists – have contributed full essays on all relevant topics, supplemented by keyword definitions of related terms. Full essays are structured uniformly to provide reader-friendly information on all aspects of Immunotoxicology, including methods of testing and analysis, characteristics of substances, the regulatory environment and the relevance of these to humans. The single A–Z format of both types of entry makes this reference book very easy to use. The Encyclopedic Reference of Immunotoxicology is intended to be a comprehensive work of reference which will provide easy access to relevant information in the fast-growing field of Immunotoxicology.
Download or read book Immunohistochemistry in Diagnostic Dermatopathology written by Mai P. Hoang and published by Cambridge University Press. This book was released on 2017-09-28 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of only a few textbooks on the market dedicated to the important role of immunohistochemistry in diagnostic dermatopathology.
