Download or read book Immobilized Enzymes in Analytical and Clinical Chemistry written by Peter W. Carr and published by John Wiley & Sons. This book was released on 1980-10-20 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Analytical Uses of Immobilized Biological Compounds for Detection Medical and Industrial Uses written by George G. Guilbault and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: On May 4-8, 1987, a NATO Advanced Research Workshop on the Analytical Uses of Immobilized Biological Compounds was held in Florence, Italy. The Director of the Workshop was Professor George G. Guilbault of the University of New Orleans, and the Co-Director was Professor Marco Mascini of the University of Florence It vas the purpose of this meeting to assemble scientists from all NATO Countries with an interest in immobilized biological compounds. to discuss - methods of immobilization - properties of immobilized compounds - enzyme electrodes and biosensors - optical devices utilizing immobilized enzymes - microbial sensors and clinical uses of immobilized enzymes - flow injection analysis using enzymes - immobilized biological compounds in chemical defense detection - pharmaceutical analysis - uses in industrial analysis - enzyme reactors - air pollution detectors - immunosensors - medical uses and applications - solid state and FET sensors Goals to be achieved by the conference were - to permit an exchange of views and experience in all these areas - to review and critically assess the state-of-the-art in these fields - to set guidelines for future research and establish collaborative projects between scientists in NATO laboratories in the above areas. Thirty-seven lectures were given by 36 speakers in all of the above areas.
Download or read book Analytical Applications of Immobilized Enzymes and Cells written by Lemuel B. Wingard and published by Elsevier. This book was released on 2014-06-28 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical Applications of Immobilized Enzymes and Cells, Volume 3 presents a survey of the interdisciplinary interactions, developments, and trends on research in immobilized enzymes and cells. This volume is comprised of eight chapters. Chapters 1 to 4 are devoted to the description of methodologies and instrumentations which are used in routine analysis. Among those described are enzyme tubes, enzyme thermistors, and enzymic or microbial electrodes. Chapter 4 provides a theoretical analysis of electrode design. Subsequent chapters focus on solid-phase enzyme immunoassays and on techniques for in vivo monitoring of metabolites; economic evaluation of the use of high-purity enzymes in analysis; and an assessment of the significance of microprocessors and computer science in enzyme based analysis. Chemists and chemical engineers will find the book very useful.
Download or read book Analytical Uses of Immobilized Enzymes written by George G. Guilbault and published by . This book was released on 1984 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Analytical Applications of Immobilized Enzyme Reactors written by S. Lam and published by Springer. This book was released on 1994 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes are bio-catalysts which effect transformation of substrates to products with high specificity. The usage of enzymes in domestic and industrial applications is well known and has been well documented since the early history of civilization. With the advances in understanding of enzymology, usage of enzymes in industrial and biotechnological pro cesses and molecular medicine has proliferated. One of the key factors in the widespread application of enzymes in modern technologies is the development of enzyme immobilization tech niques, which overcome certain practical, functional and economic con straints. Many natural enzymes can be stabilized by immobilization on solid matrices, with most of the activity retained, for a variety of applica tions. An important application of immobilized enzymes is in liquid chromatography. In the last decade, post-column enzyme detection has become established as an important discipline in liquid chromatography. The new detection approach offers more sensitive and specific ways for measuring major classes of biomolecules. Reactors are fabricated by packing the immobilized enzymes into small columns, which can be placed immediately after an HPLC column.
Download or read book Immobilized Enzymes in Analytical Chemistry written by Larry D. Bowers and published by . This book was released on 1980 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Immobilized Enzymes in Analytical and Clinical Chemistry written by Peter W. Carr and published by John Wiley & Sons. This book was released on 1980-10-20 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Application of Immobilized Enzymes in Analytical Chemistry written by Richard Kwasi Owusu and published by . This book was released on 1985 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Immobilized Enzymes in Medicine written by Vladimir P. Torchilin and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: The application of immobilized enzymes in medicine is the main objective of this book. The author reviews natural and synthetic carriers for enzyme immobilization, chemistry of enzyme binding, and in-vitro and in-vivo properties of immobilized enzymes. Four chapters are dedicated to clinical use of immobilized enzymes.
Download or read book Immobilization of Enzymes and Cells written by José M. Guisán and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation—from the laboratory to industry—of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of protocols for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few protocols are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related properties.
Download or read book Methods of Enzymatic Analysis Fundamentals written by Hans Ulrich Bergmeyer and published by . This book was released on 1983 with total page 608 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enzymology in the Practice of Laboratory Medicine written by Philip Blume and published by Academic Press. This book was released on 2014-05-10 with total page 433 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymology in the Practice of Laboratory Medicine focuses on disease processes, enzymatic reactions and activities, and diagnosis and management of diseases. The selection first offers information on developments in the search for mechanisms of enzymic catalysis; application of the spin labeling technique to biological membranes; and the properties of membrane bound enzymes involved in energy transduction processes. Discussions focus on possible clinical interrelationships of membrane function, mitochondrial membrane transactions, mechanical details of chymotryptic catalysis, and physical nature of globular proteins. The text then elaborates on metalloenzymes, enzyme measurement, and enzymology, clinical chemistry, and development of fast analyzers. The publication examines the analytical uses of immobilized enzymes; origin of serum enzymes and the basis for their variation; and the screening methods for glycolytic enzyme deficiencies in red blood cells. Topics include factors contributing to normal variation of enzyme activity, catabolism of enzymes, mechanism of release from cells to plasma, and determination of urea in blood and urine. The selection is a dependable reference for researchers interested in the application of enzymology in laboratory medicine.
Download or read book Biomedical Applications of Immobilized Enzymes and Proteins written by Thomas Ming Swi Chang and published by Springer Science & Business Media. This book was released on 2013-11-21 with total page 433 pages. Available in PDF, EPUB and Kindle. Book excerpt: I) ADSORPTION EEEEEEEE E E carrier 2) COVALENT LINKAGE a) Insoluble support b) Intermolecular linkage N'E~ ~~ c) Soluble support 0 \:)....m 3) tM TRIX (MOLECULAR) ENTRAPMENT ~~~~~;;..,J~-polymer matrix 4) ENCAPSULATION membrane FIGURE I. Classification of immobilized enzymes. Covalently linked, adsorbed, and matrix-entrapped enzymes represent stage II, research on the microenvironment. Microencapsulation represents stage III, research on the intracellular environment. Further subdivision of microencapsulated enzymes will be found in Chapter 12. 4 T. M. S. CHANG matrix entrapment. In this section, detailed discussions will center on clinical analysis, urine analysis, monitoring of environmental pollution, radioimmune assay, enzyme-linked immunosorbent assay, enzyme electrodes, and other approaches involving immobilized enzymes, antibodies, and antigens. In the final section, research workers describe and discuss the perspectives of immobilized enzymes and proteins. Here, they speculate on the future potential of possible approaches, even though these may not have been extensively studied or tested at the laboratory stage. The biomedical applications of enzymes and proteins, especially in the thera peutic area, is in a very early stage of development. Much remains to be explored and studied, and the area is wide open for investigators interested in original research in a new interdisciplinary area. References Chang, T. M. S., 1972, Artificial Cells, Charles C. Thomas, Publisher, Springfield, Ill. Dunlop, R. B. (ed.), 1974, Immobilized Biochemicals and Affinity ChrOTIULtography, Plenum Press, N ew York.
Download or read book Uses of Immobilized Biological Compounds written by George G. Guilbault and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 578 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Uses of Immobilized Biological Compounds the reader will find a comprehensive survey of the field written by acknowledged experts who met in Brixen, Italy, between May 9 and 14, 1993 for a NATO Advanced Research Workshop devoted to the topic. The resulting volume presents a critical review of the latest results in the area and sets guidelines for future research. The 53 reports presented here cover: (A) General Aspects of Immobilizing Biological Compounds; (B) Medical, Clinical and Pharmaceutical Applications; (C) Electrochemical Biosensors; (E) Defense Applications; (F) Immunosensors and Receptors; (G) Food, Environmental, Clinical and Analytical Applications; and (H) Biotechnology and Marketing. In short, all aspects of the area are presented, in a compact format which will appeal to undergraduates, technicians, and professional scientists in the food, clinical, environmental, pharmaceutical and industrial fields.
Download or read book Immobilization of Enzymes and Cells written by and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Bioanalytical Applications of Enzymes written by Clarence H. Suelter and published by John Wiley & Sons. This book was released on 2009-09-25 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: Details the latest advances in bioanalytical applications using enzymes--techniques that are becoming increasingly important in analysis, synthesis, manufacturing and medical diagnosis. Consists of seven articles which cover: enzyme labeled antibodies in bioassays, DNA restriction enzymes and RFLPs in medicine, enzyme-labeled probes for nucleic acid hybridization, unique methodologies of immobilized proteins in bioanalytical systems, dry reagent chemistry fundamentals, the theory and applications of enzyme electrode biosenors, and advances in enzymatically coupled field effect transistors.
Download or read book Immobilized Enzymes in Food and Microbial Processes written by Alfred Olson and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last five years the potential value of immobilized enzymes has captured the imagination of an increasing number of scientists and engineers. The concept of being able to create an immobilized derivative of an enzyme which has long-term stability and is able to be recovered and reused is fascinating, to say the least. Since the industrial application of enzymes has been mostly in the food and microbial process industry it is not surprising that many of the applications of immobilized enzymes considered for commercial development fall within the area of this industry. It is for this reason that we organized a symposium on immobilized enzymes for the 166th National Meeting of the American Chemical Society. Appropriately, the symposium was jointly sponsored by the Division of Agricultural and Food Chemistry and the Division of Microbial Chemistry and Technology. Although there were at least half a dozen symposia at other meetings on various aspects of immobilized enzyme technology in the preceding ten months none had specifically addressed themselves to food and microbial processes and none had been held at a meeting such as the National ACS gathering, which is not only large but multidisciplinary. The enthusiastic response to this symposium prompted us, at the invita tion of Plenum Press, to publish the proceedings of this symposium.
