Download or read book Identification Evolution and Expression of the Trace Amine Associated Receptor TAAR Gene Family in Atlantic Salmon Salmo Salar written by Jordan Anthony Tessarolo and published by . This book was released on 2014 with total page 95 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is widely hypothesized that Atlantic salmon are imprinted at a young age with olfactory cues, which they use as a guide in order to return to their natal streams to spawn. However, the molecular mechanism(s) behind this biological phenomenon remain unknown. Therefore, in order to better understand imprinting and homing in Atlantic salmon, it is important to characterize the repertoire of olfactory receptors in thisspecies. A search of the first assembly of the Atlantic salmon genome revealed 27 putatively functional trace amine-associated receptor (TAAR) genes and 25 putative TAAR pseudo-genes. Genetic mapping, phylogenetic analysis, binding-site prediction, and quantitative PCR were performed using the Atlantic salmon TAAR genes. The identification of this gene family in Atlantic salmon will facilitate additional studiesinvolving olfaction and homing such as determining the range of allelic variation in olfactory receptors genes of different salmon populations.
Download or read book Chemosensory Transduction written by Frank Zufall and published by Academic Press. This book was released on 2016-02-18 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by leaders in the field of chemosensation, Chemosensory Transduction provides a comprehensive resource for understanding the molecular mechanisms that allow animals to detect their chemical world. The text focuses on mammals, but also includes several chapters on chemosensory transduction mechanisms in lower vertebrates and insects. This book examines transduction mechanisms in the olfactory, taste, and somatosensory (chemesthetic) systems as well as in a variety of internal sensors that are responsible for homeostatic regulation of the body. Chapters cover such topics as social odors in mammals, vertebrate and invertebrate olfactory receptors, peptide signaling in taste and gut nutrient sensing. Includes a foreword by preeminent olfactory scientist Stuart Firestein, Chair of Columbia University’s Department of Biological Sciences in New York, NY. Chemosensory Transduction describes state-of-the-art approaches and key findings related to the study of the chemical senses. Thus, it serves as the go-to reference for this subject for practicing scientists and students with backgrounds in sensory biology and/or neurobiology. The volume will also be valuable for industry researchers engaged in the design or testing of flavors, fragrances, foods and/or pharmaceuticals. Provides a comprehensive overview for all chemosensory transduction mechanisms Valuable for academics focused on sensory biology, neurobiology, and chemosensory transduction, as well as industry researchers in new flavor, fragrance, and food testing Edited by leading experts in the field of olfactory transduction Focuses on mammals, but lower vertebrates and invertebrate model systems are also included
Download or read book Identification Evolution and Expression of Three Olfactory Gene Families in Atlantic Salmon Salmo Salar written by Kimberley Anne Johnstone and published by . This book was released on 2011 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: It has been hypothesized that salmonids use olfactory cues to return to their natal rivers and streams. However, the key components of the molecular pathway involved in imprinting and homing are still unknown. If odorants are involved in salmon homing migration then olfactory receptors should play a critical role in the dissipation of information from the environment to the fish. To understand the molecular basis for imprinting and homing in Atlantic salmon (Salmo salar), it is important to identify and characterize the olfactory receptors in the Atlantic salmon genome. Aquatic chemical cues are received through the salmon nares and into the nasal cavity that contains a single olfactory organ, olfactory rosette. The olfactory rosette contains sensory neurons, which are thought to express only one olfactory receptor. In this study, three major superfamilies of fish olfactory receptors (MOR, ora and OlfC) were examined. To identify the olfactory genes in Atlantic salmon several genomic and bioinformatic techniques were used. First, an Atlantic salmon bacterial artificial chromosome (BAC) library was screened with probes designed from previously identified fish olfactory receptor sequences. Then a selected number of hybridization positive BACs containing olfactory receptors were shotgun cloned and sequenced. From these BAC sequences, two ora genes and 55 OlfC genes were identified in Atlantic salmon. The second technique used to identify olfactory receptors in Atlantic salmon was a bioinformatic approach that involved screening a 3-fold Atlantic salmon genome sequence for olfactory receptors. Using this approach, 24 MOR and the remaining five ora genes were identified, as well as another 24 partial genes or pseudogenes. As a first step to understand how olfactory receptors are involved in imprinting and homing, a suite of olfactory receptors were selected to examine the expression profiles of these genes across different life stages and life histories of wild Atlantic salmon from Newfoundland, Canada. Seven differentially expressed OlfC genes were identified in juvenile anadromous salmon compared to returning adult salmon. From this research, I hypothesize that OlfC genes may play an important role in the imprinting of home stream water olfactory cues in anadromous Atlantic salmon.
Download or read book Identification and Annotation of Full length Genes in Atlantic Salmon Salmo Salar written by Jong S. Leong and published by . This book was released on 2010 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Large-scale expressed sequence tags (ESTs) in Atlantic salmon (Salmo salar) are examined to answer questions regarding salmonid transcriptomes. ESTs represent raw and incomplete gene sequences that need to be read, assembled and analyzed with computer software. The goal of this thesis was to develop an automatically curated and publicly accessible set of annotated full-length genes, representing a near-complete transcript set for Salmo salar. In turn, these genes provide the framework for studies in gene expression, conservation, and molecular evolution. The work presented here also touches on the results of a molecular evolution study, as an example of how full-length gene identification can be used to answer biological questions. Previous to this study, a limited number of Atlantic salmon cDNA libraries and ESTs were known. To further the goal of determining complete gene sequences, highly enriched full-length cDNA libraries and full-length libraries were created and sequenced, resulting in the ability to identify a large number of full-length reference genes. Together, all libraries represent a diverse pool of transcriptome sequences for Salmo salar. The goal of producing an accurate large-scale full-length gene set on a duplicated genome is not trivial. Complete systems for this objective do not readily exist. EST sequencing, EST assembly, and data storage, are just a few of the initial computational issues that are addressed. Once these issues are resolved, the multi-step workflow of full-length gene determination is described. The final challenge involving the development of a concise and universally accessible system for visualization is discussed. The resulting computational framework that has been developed is shown to be able to handle the intricacies and the size of a duplicated salmonid genome. It has been largely accepted that Atlantic salmon have undergone a recent genome duplication. Gene paralogs provide one source of evidence for this event. Analysis of paralogs revealed signatures of asymmetric evolution possibly due to relaxation of selective pressure. This thesis provides a complete Bioinformatics analysis pipeline to analyze and to visualize a set of full-length reference genes for Atlantic salmon. Using full-length genes as a framework, the topic of molecular evolution was addressed to show evidence of asymmetrical evolution among gene duplicates. The full-length reference genes, along with ESTs and all putative transcripts, have been made publicly available. These results serve as a valuable genomic resource for next-generation sequencing and for all other salmonid research endeavours.
Download or read book Isolation and Characterization of Atlantic Salmon Salmo Salar Olfactory Receptor Genes written by Jill Elizabeth Thorpe and published by . This book was released on 2000 with total page 94 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Evolutionary Biology of the Atlantic Salmon written by Tomislav Vladić and published by CRC Press. This book was released on 2015-08-10 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atlantic salmon (Salmo salar, L) is an anadromous species contributing to major fishery catches and comprising a significant part of the aquaculturally produced fish for human consumption. The aim of this book is to incite the interest in the field of knowledge gathered from a broad scope of disciplines that have investigated this fish species. Und
Download or read book The Atlantic Salmon written by Eric Verspoor and published by John Wiley & Sons. This book was released on 2007-05-21 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atlantic Salmon is a cultural icon throughout its North Atlantic range; it is the focus of probably the World’s highest profile recreational fishery and is the basis for one of the World’s largest aquaculture industries. Despite this, many wild stocks of salmon are in decline and underpinning this is a dearth of information on the nature and extent of population structuring and adaptive population differentiation, and its implications for species conservation. This important new book will go a long way to rectify this situation by providing a thorough review of the genetics of Atlantic salmon. Sponsored by the European Union and the Atlantic Salmon Trust, this book comprises the work of an international team of scientists, carefully integrated and edited to provide a landmark book of vital interest to all those working with Atlantic salmon.
Download or read book Identification and Annotation of Full length Genes in Atlantic Salmon Salmo Salar written by Jong S. Leong and published by . This book was released on 2010 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Large-scale expressed sequence tags (ESTs) in Atlantic salmon (Salmo salar) are examined to answer questions regarding salmonid transcriptomes. ESTs represent raw and incomplete gene sequences that need to be read, assembled and analyzed with computer software. The goal of this thesis was to develop an automatically curated and publicly accessible set of annotated full-length genes, representing a near-complete transcript set for Salmo salar. In turn, these genes provide the framework for studies in gene expression, conservation, and molecular evolution. The work presented here also touches on the results of a molecular evolution study, as an example of how full-length gene identification can be used to answer biological questions. Previous to this study, a limited number of Atlantic salmon cDNA libraries and ESTs were known. To further the goal of determining complete gene sequences, highly enriched full-length cDNA libraries and full-length libraries were created and sequenced, resulting in the ability to identify a large number of full-length reference genes. Together, all libraries represent a diverse pool of transcriptome sequences for Salmo salar. The goal of producing an accurate large-scale full-length gene set on a duplicated genome is not trivial. Complete systems for this objective do not readily exist. EST sequencing, EST assembly, and data storage, are just a few of the initial computational issues that are addressed. Once these issues are resolved, the multi-step workflow of full-length gene determination is described. The final challenge involving the development of a concise and universally accessible system for visualization is discussed. The resulting computational framework that has been developed is shown to be able to handle the intricacies and the size of a duplicated salmonid genome. It has been largely accepted that Atlantic salmon have undergone a recent genome duplication. Gene paralogs provide one source of evidence for this event. Analysis of paralogs revealed signatures of asymmetric evolution possibly due to relaxation of selective pressure. This thesis provides a complete Bioinformatics analysis pipeline to analyze and to visualize a set of full-length reference genes for Atlantic salmon. Using full-length genes as a framework, the topic of molecular evolution was addressed to show evidence of asymmetrical evolution among gene duplicates. The full-length reference genes, along with ESTs and all putative transcripts, have been made publicly available. These results serve as a valuable genomic resource for next-generation sequencing and for all other salmonid research endeavours.
Download or read book Evolution Illuminated written by Stephen C. Stearns and published by Oxford University Press. This book was released on 2003-11-27 with total page 521 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book will appeal to investigators in each of the scientific disciplines it integrates--evolutionary biology, ecology, salmonid biology, management, and conservation. Variation in salmonids can be used to illustrate virtually all evolutionary questions, and so the work will also attract general scientific interest by ecologists and evolutionary and conservation biologists.
Download or read book Evolution and Characterization of the Fatty Acid binding Proteins fabps in Atlantic Salmon Salmo Salar written by Yuk Yin Lai and published by . This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is suggested that gene or genome duplication is the driving force in evolution that leads to speciation. Two models, the classical model and the duplication- degeneration-complementation (DDC) model, have been proposed on the fates of gene duplicates resulting from either a gene or a genome duplication event. The classical model suggests that one of the gene duplicates might result in loss of function (non- functionalization) or gain of a new function (neo-functionalization) depending on whether the accumulated mutations over the years are deleterious or beneficial to the organism. In the DDC model, it is proposed that each of the gene duplicates might accumulate different deleterious mutations in the regulatory region of the gene, such that these genes partition the ancestral gene function (sub-functionalization). Combinations of the phylogenetic analysis of many gene families support that salmonids have undergone two additional whole genome duplications compared to the mammals, one occurred in the common ancestors of teleosts and another happened in the common ancestor of salmonids approximately 25-120 million years ago. In this thesis, the evolution of the fatty acid-binding protein (fabp) family in fish and salmonids was examined. I have characterized eighteen unique fabp genes in Atlantic salmon. These include the seven fabp sub-families described previously in fish. Phylogenetic analyses and conservation of synteny support the two whole genome duplication events in the common ancestors of teleosts and salmonids and indicate when gene losses occurred. Genetic mapping of fabp gene duplicates to homeologous chromosomes in Atlantic salmon also support that they arose by the 4R genome duplication. I also searched for the signatures of neo-functionalization and sub-functionalization by calculating dN/dS ratios, examining the nature of amino acid substitutions and expression patterns, and suggested the fates of fabp gene duplicates in Atlantic salmon. Overall, the findings of this project provide insight into the evolutionary processes at play in salmonid genomes.
Download or read book The Fate of Duplicated Regions of the Atlantic Salmon Salmo Salar Genome microform written by Leslie Mitchell and published by Library and Archives Canada = Bibliothèque et Archives Canada. This book was released on 2004 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gene and genome duplications have played a major role in vertebrate evolution. Salmonids provide a useful resource for studying the consequences of these events as their common ancestor underwent a genome duplication between 25 and 120 miliion years ago. To understand how a genome reorganizes itself to cope with duplicated chromosomes and the importance of gene duplications for evolution and adaptation, homeologous regions of the Atlantic salmon genome were identified and studied within a large insert, genomic BAC library; these BACs contain the metallothionen event. A BAC from each region was subsequently shotgun subcloned and sequenced. Sequence analysis revealed the presence of 10 genes, retaining their collinearity between the BAcs, although pseudogenization events have occurred in one of the duplicate loci in two instances. Comparative genomic analysis revealed the existence of extraordinary conservation of syntney over time.
Download or read book Evolutionary Genetics of Atlantic Salmon Salmo Salar L written by Anti Vasemägi and published by . This book was released on 2004 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Family specific Differences in Growth Rate and Hepatic Gene Expression in Triploid Growth Hormone GH Transgenic Atlantic Salmon Salmo Salar L written by Qingheng Xu and published by . This book was released on 2012 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Aquaculture Genome Technologies written by Zhanjiang (John) Liu and published by John Wiley & Sons. This book was released on 2008-02-28 with total page 579 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genomics is a rapidly growing scientific field with applications ranging from improved disease resistance to increased rate of growth. Aquaculture Genome Technologies comprehensively covers the field of genomics and its applications to the aquaculture industry. This volume looks to bridge the gap between a basic understanding of genomic technology to its practical use in the aquaculture industry.
Download or read book Molecular Basis of Odor written by John E. Amoore and published by . This book was released on 1970 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book On Food and Cooking written by Harold McGee and published by Simon and Schuster. This book was released on 2007-03-20 with total page 898 pages. Available in PDF, EPUB and Kindle. Book excerpt: A kitchen classic for over 35 years, and hailed by Time magazine as "a minor masterpiece" when it first appeared in 1984, On Food and Cooking is the bible which food lovers and professional chefs worldwide turn to for an understanding of where our foods come from, what exactly they're made of, and how cooking transforms them into something new and delicious. For its twentieth anniversary, Harold McGee prepared a new, fully revised and updated edition of On Food and Cooking. He has rewritten the text almost completely, expanded it by two-thirds, and commissioned more than 100 new illustrations. As compulsively readable and engaging as ever, the new On Food and Cooking provides countless eye-opening insights into food, its preparation, and its enjoyment. On Food and Cooking pioneered the translation of technical food science into cook-friendly kitchen science and helped birth the inventive culinary movement known as "molecular gastronomy." Though other books have been written about kitchen science, On Food and Cooking remains unmatched in the accuracy, clarity, and thoroughness of its explanations, and the intriguing way in which it blends science with the historical evolution of foods and cooking techniques. Among the major themes addressed throughout the new edition are: · Traditional and modern methods of food production and their influences on food quality · The great diversity of methods by which people in different places and times have prepared the same ingredients · Tips for selecting the best ingredients and preparing them successfully · The particular substances that give foods their flavors, and that give us pleasure · Our evolving knowledge of the health benefits and risks of foods On Food and Cooking is an invaluable and monumental compendium of basic information about ingredients, cooking methods, and the pleasures of eating. It will delight and fascinate anyone who has ever cooked, savored, or wondered about food.
Download or read book Probiotics and Prebiotics in Animal Health and Food Safety written by Diana Di Gioia and published by Springer. This book was released on 2018-02-27 with total page 275 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses the role of probiotics and prebiotics in maintaining the health status of a broad range of animal groups used for food production. It also highlights the use of beneficial microorganisms as protective agents in animal derived foods. The book provides essential information on the characterization and definition of probiotics on the basis of recently released guidelines and reflecting the latest trends in bacterial taxonomy. Last but not least, it discusses the concept of “dead” probiotics and their benefits to animal health in detail. The book will benefit all professors, students, researchers and practitioners in academia and industry whose work involves biotechnology, veterinary sciences or food production.
