Download or read book Genetic Study of Resistance to Charcoal Rot and Fusarium Stalk Rot Diseases of Sorghum written by Adedayo Adeyanju and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Fusarium stalk rot and charcoal rot caused by Fusarium thapsinum and Macrophomina phaseolina respectively are devastating global diseases in sorghum that lead to severe quality and yield loss each year. In this study, three sets of interrelated experiments were conducted that will potentially lead to the development of resistance based control option to these diseases. The first experiment was aimed at identifying sources of resistance to infection by M. phaseolina and F. thapsinum in a diverse panel of 300 sorghum genotypes. The genotypes were evaluated in three environments following artificial inoculation. Out of a total of 300 genotypes evaluated, 95 genotypes were found to have resistance to M. phaseolina and 77 to F. thapsinum of which 53 genotypes were resistant to both pathogens. In the second experiment, a set of 79,132 single nucleotide polymorphisms (SNPs) markers were used in an association study to identify genomic regions underlying stalk rot resistance using a multi-locus mixed model association mapping approach. We identified 14 loci associated with stalk rot and a set of candidate genes that appear to be involved in connected functions controlling plant defense response to stalk rot resistance. The associated SNPs accounted for 19-30% of phenotypic variation observed within and across environments. An analysis of associated allele frequencies within the major sorghum subpopulations revealed enrichment for resistant alleles in the durra and caudatum subpopulations compared with other subpopulations. The findings suggest a complicated molecular mechanism of resistance to stalk rots. The objective of the third experiment was to determine the functional relationship between stay-green trait, leaf dhurrin and soluble sugar levels and resistance to stalk rot diseases. Fourteen genotypic groups derived from a Tx642 × Tx7000 RIL population carrying combinations of stay-green quantitative trait loci were evaluated under three environments in four replications. The stg QTL had variable effects on stalk rot disease. Genotypes carrying stg1, stg3, stg1,3 and stg1,2,3,4 expressed good levels of resistance to M. phaseolina but the combination of stg1 and stg3 was required to express the same level of resistance to F. thapsinum. Other stg QTL blocks such as stg2 and stg4 did not have any impact on stalk rot resistance caused by both pathogens. There were no significant correlations between leaf dhurrin, soluble sugar concentration, and resistance to any of the pathogens.

Download or read book Sorghum Root and Stalk Rots written by Gloria Rosenberg and published by . This book was released on 1984 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Sorghum and Millets Diseases written by John F. Leslie and published by John Wiley & Sons. This book was released on 2008-02-28 with total page 524 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work is based on the Mexico 2000 meeting under the auspices of ICRISAT (International Crops Research Institute for Semi-Arid Tropics) and INTSORMIL (International Sorghum and Millet Collaborative Research Support Program). Sorghum and millet are very important agronomic crops in many parts of the world, specifically in the semi-arid regions in warm areas. The crops are of great significance in supplying food and feed in the developing areas of Latin America, Africa, and Asia.

Download or read book Disease Analysis Through Genetics and Biotechnology written by John F. Leslie and published by Wiley-Blackwell. This book was released on 1995 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: Focusing on the most important sorghum and millet diseases, this book is the result of an international conference on diseases of sorghum and millet from different growing regions of the world. New research approaches to improve disease management strategies are included.

Download or read book Genetic Analysis of Yield Its Components and Resistance to Charcoal Rot in Rabi Sorghum Sorghum Bicolor L Moench written by GURARAJA RAO M. R and published by . This book was released on 1992 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Analysis of Resistance to Fusarium Stalk Rot in Grain Sorghum Sorghum Bicolor L Moench written by Tesfaye Teferra Tesso and published by . This book was released on 2002 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic Analysis of Yield Components and Charcoal Rot Macrophomina Phaseolina Tassi Goid Resistance in Rabi Sorghum sorghum Bicolor L Moench written by K. Hussain Sahib and published by . This book was released on 1985 with total page 253 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Compendium of Sorghum Diseases written by Richard A. Frederiksen and published by American Phytopathological Society. This book was released on 2000 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt: Specialists from around the world provide a basic diagnostic tool for identifying diseases of the grain and related problems that anyone with a technical background in plant disease diagnosis should be able to use. They cover the many biotic and the few abiotic diseases, beneficial microorganisms, insect pests, and methods of control. Color photographs show clearly the symptoms. The extensive glossary does not indicate pronunciations. The first edition appeared in 1986. Annotation copyrighted by Book News, Inc., Portland, OR

Download or read book Cultural Studies to Determine Resistance to Fusarium Moniliforme Stalk and Root Rot Disease of Sorghum written by Moise Medjo-Me-Nkembe and published by . This book was released on 1982 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Understanding the Physiological and Molecular Aspects of Charcoal Rot Resistance Mechanisms in Sorghum and Soybean written by Afsana Noor and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Charcoal rot (CR) of soybean (Glycine max (L.) Merr.) and sorghum (Sorghum bicolor (L.) Moench) is caused by the hemibiotrophic soilborne fungus Macrophomina phaseolina (MP) and is an important pathogen in the midwestern United States. Complex molecular mechanisms underlie the interaction of MP with these two hosts, which impedes resistance breeding. To select for charcoal rot resistance, a thorough understanding of the host's physiological and molecular responses to MP along with screening of genotypes with resistance to CR is essential. To understand MP induced host's physiological and molecular responses, first we investigated MP-induced oxidative stress-mediated senescence by using the reactive oxygen species (ROS) scavenger ascorbic acid in soybean seedlings. Three soybean isolates of MP were tested for their sensitivity to ascorbic acid using an in-vitro assay. An in-planta soybean cut-stem assay was used for the exogenous application of ascorbic acid (oxidized and reduced form) following inoculation with MP. A ROS (H2O2) quantification assay was used to validate H2O2 induced by MP and ascorbic acid pre-treatment. All three MP isolates were sensitive to ascorbic acid concentrations of ≥15 mM. Ascorbic acid (10mM) pre-treatment following MP inoculation reduced CR lesion length compared to inoculated treatment. MP induced a significantly higher H2O2 than ascorbic acid pre-treated inoculated plant. Second, through comparative transcriptomics, MP-resistant and susceptible soybean genotypes revealed contrasted responses to MP-induced senescence. Gene Ontology and pathway analysis showed MP-induced receptor kinase like genes in both genotypes while down-regulated defense related antioxidant, hormonal, and other metabolic pathways in both genotypes. Ascorbic acid pre-treatment induced a more significant number of photosynthesis genes in both genotypes. Hydrogen peroxide pre-treatment following inoculation showed up-regulation of oxidative stress responsive pathways while down-regulated photosynthesis and hormonal signal transduction pathways. Third, the NAM phenotyping for CR resistance results of location- and year-wise data showed strong genotype by environment interactions. Overall, using MP screening, charcoal rot resistance phenotyping in the NAM parental lines revealed the genotype SC1103 as the most resistant line and Segaolane and Macia as the most susceptible. The SC1103 NAM family-derived population can be used for charcoal rot resistance in association studies to map charcoal rot resistance.

Download or read book Dissecting the Molecular Responses of Sorghum Bicolor to Macrophomina Phaseolina Infection written by Y. M. Ananda Yapa Bandara and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Charcoal rot, caused by the necrotrophic fungus, Macrophomina phaseolina (Tassi) Goid., is an important disease in sorghum (Sorghum bicolor (L.) Moench). The molecular interactions between sorghum and M. phaseolina are poorly understood. In this study, a large-scale RNA-Seq experiment and four follow-up functional experiments were conducted to understand the molecular basis of charcoal rot resistance and/or susceptibility in sorghum. In the first experiment, stalk mRNA was extracted from charcoal-rot-resistant (SC599) and susceptible (Tx7000) genotypes and subjected to RNA sequencing. Upon M. phaseolina inoculation, 8560 genes were differentially expressed between the two genotypes, out of which 2053 were components of 200 known metabolic pathways. Many of these pathways were significantly up-regulated in the susceptible genotype and are thought to contribute to enhanced pathogen nutrition and virulence, impeded host basal immunity, and reactive oxygen (ROS) and nitrogen species (RNS)-mediated host cell death. The paradoxical hormonal regulation observed in pathogen-inoculated Tx7000 was characterized by strongly upregulated salicylic acid and down-regulated jasmonic acid pathways. These findings provided useful insights into induced host susceptibility in response to this necrotrophic fungus at the whole-genome scale. The second experiment was conducted to investigate the dynamics of host oxidative stress under pathogen infection. Results showed M. phaseolina's ability to significantly increase the ROS and RNS content of two charcoal-rot-susceptible genotypes, Tx7000 and BTx3042. Over-accumulation of nitric oxide (NO) in stalk tissues in the pathogen-inoculated susceptible genotypes was confirmed using a NO-specific fluorescent probe and confocal microscopy. Significantly increased malondialdehyde content confirmed the enhanced oxidative stress experienced by the susceptible genotypes after pathogen inoculation. These findings suggested the contribution of oxidative stress-associated induced cell death on charcoal rot susceptibility under infection. In the third functional experiment, the behavior of the sorghum antioxidant system after pathogen inoculation was investigated. M. phaseolina significantly increased the glutathione s- transferase (GST), glutathione peroxidase (GPX), glutathione reductase (GR), and peroxidase activities of the susceptible genotypes (Tx7000, BTx3042) but not in the resistant genotypes (SC599, SC35). Increased activities of these enzymes in susceptible genotypes may contribute to reduced oxidative stress thus lowering charcoal rot susceptibility. The fourth functional experiment was designed to quantify induced host-derived cell wall degrading enzymes (CWDEs) using crude enzyme mixtures from stalks. A gel diffusion assay revealed significantly increased pectinesterase activity in pathogen-inoculated Tx7000 and BTx3042 while significantly increased polygalacturonase activity was determined by absorbance. Fluorimetric determination of cell extracts revealed significantly increased cellulose degrading enzyme activity in M. phaseolina-inoculated Tx7000 and BTx3042. These findings revealed the pathogen's ability to promote charcoal rot susceptibility in grain sorghum through induced host CWDEs. The last functional study was designed to profile the stalk tissue lipidome of Tx7000 and SC599 after M. phaseolina inoculation using automated direct infusion electrospray ionization-triple quadrupole mass spectrometry (ESI-MS/MS). M. phaseolina significantly decreased the phytosterol, phosphatidylserine, and ox-lipid contents in Tx7000 while significantly increasing stigmasterol:sitosterol ratio. Except for ox-lipid content, none of the above was significantly affected in resistant SC599. Results suggested the lethal impacts of M. phaseolina inoculation on plastid- and cell- membrane integrity and the lipid-based signaling capacity of Tx7000. Findings shed light on the host lipid classes that contribute to induced charcoal rot susceptibility in grain sorghum.

Download or read book Molecular Identification of Fungi written by Youssuf Gherbawy and published by Springer Science & Business Media. This book was released on 2010-03-03 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fungi enjoy great popularity in pharmaceutical, agricultural, and biotechnological applications. Recent advances in the decipherment of whole fungal genomes promise an acceleration of these trends. This timely book links scientists from different parts of the world who are interested in the molecular identification of fungi combined with the exploration of the fungal biodiversity in different ecosystems. It provides a compendium for scientists who rely on a rapid and reliable detection of fungal specimens in environmental as well as clinical resources in order to ensure the benefit of industrial and clinical applications. Chapters focus on the opportunities and limits of the molecular marker-mediated identification of fungi. Various methods, procedures and strategies are outlined. Furthermore, the book offers an update of the current progress in the development of fungal molecular techniques, and draws attention to potential and associated problems, as well as integrating theory and practice.

Download or read book Genetic Improvement of Bioenergy Crops written by Wilfred Vermerris and published by Springer Science & Business Media. This book was released on 2008-08-02 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ethanol as an alternative fuel is receiving a lot of attention because it addresses concerns related to dwindling oil supplies, energy independence, and climate change. The majority of the ethanol in the US is produced from corn starch. With the US Department of Energy’s target that 30% of the fuel in the US is produced from renewable resources by 2030, the anticipated demand for corn starch will quickly exceed the current production of corn. This, plus the concern that less grain will become available for food and feed purposes, necessitates the use of other feedstocks for the production of ethanol. For the very same reasons, there is increasing research activity and growing interest in many other biomass crops. Genetic Improvement of Bio-Energy Crops focuses on the production of ethanol from lignocellulosic biomass, which includes corn stover, biomass from dedicated annual and perennial energy crops, and trees as well as a number of important biomass crops. The biomass is typically pretreated through thermochemical processing to make it more amenable to hydrolysis with cellulolytic enzymes. The enzymatic hydrolysis yields monomeric sugars that can be fermented to ethanol by micro-organisms. While much emphasis has been placed on the optimization of thermo-chemical pretreatment processes, production of more efficient hydrolytic enzymes, and the development of robust microbial strains, relatively little effort has been dedicated to the improvement of the biomass itself.

Download or read book Sorghum written by Ignacio A. Ciampitti and published by John Wiley & Sons. This book was released on 2020-01-22 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sorghum is among the top five cereals and one of the key crops in global food security efforts. Sorghum is a resilient crop under high-stress environments, ensuring productivity and access to food when other crops fail. Scientists see the potential of sorghum as a main staple food in a future challenged by climate change. The contributors provide a comprehensive review of sorghum knowledge. The discussion covers genetic improvements, development of new hybrids, biotechnology, and physiological modifications. Production topics include water and nutrient management, rotations, and pest control. Final end uses, sorghum as a bioenergy crop, markets, and the future of sorghum are presented. IN PRESS! This book is being published according to the “Just Published” model, with more chapters to be published online as they are completed.

Download or read book Compendium of Soybean Diseases and Pests written by and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Sorghum Molecular Breeding written by R. Madhusudhana and published by Springer. This book was released on 2015-08-20 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an up-to-date overview of international research work on sorghum. Its comprehensive coverage of our current understanding of transgenic development in sorghum and the strategies that are being applied in molecular breeding make this book unique. Important areas such as genetic diversity, QTL mapping, heterosis prediction, genomic and bioinformatics resources, post-genome sequencing developments, molecular markers development using bioinformatics tools, genetic transformation and transgenic research are also addressed. The availability of the genome sequence along with other recent developments in sequencing and genotyping technologies has resulted in considerable advances in the area of sorghum genomics. These in turn have led to the generation of a large number of DNA-based markers and resulted in the identification and fine mapping of QTL associated with grain yield, its component traits, biotic and abiotic stress tolerance as well as grain quality traits in sorghum. Though a large volume of information has accumulated over the years, especially following the sequencing of the sorghum genome, until now it was not available in a single reference resource. This book fills that gap by documenting advances in the genomics and transgenic research in sorghum and presenting critical reviews and future prospects. “Sorghum Molecular Breeding” is an essential guide for students, researchers and managers who are involved in the area of molecular breeding and transgenic research in sorghum and plant biologists in general.

Download or read book Genes Involved in Plant Defense written by Thomas Boller and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many fungi and bacteria that associate with plants are potentially harmful and can cause disease, while others enter into mutually beneficial sym bioses. Co-evolution of plants with pathogenic and symbiotic microbes has lead to refined mechanisms of reciprocal recognition, defense and counter defense. Genes in both partners determine and regulate these mechanisms. A detailed understanding of these genes provides basic biological insights as well as a starting point for developing novel methods of crop protection against pathogens. This volume deals with defense-related genes of plants and their regulation as well as with the genes of microbes involved in their interaction with plants. Our discussion begins at the level of populations and addresses the complex interaction of plant and microbial genes in multigenic disease resistance and its significance for crop protection as compared to mono genic resistance (Chap. 1). Although monogenic disease resistance may have its problems in the practice of crop protection, it is appealing to the experimentalist: in the so-called gene-for-gene systems, single genes in the plant and in the pathogen specify the compatibility or incompatibility of an interaction providing an ideal experimental system for studying events at the molecular level (Chaps. 2 and 4). Good progress has been made in identifying viral, bacterial, and fungal genes important in virulence and host range (Chaps. 3-6). An important aspect of plant-microbe interactions is the exchange of chemical signals. Microbes can respond to chemical signals of plant origin.