Download or read book Experiments in the Purification and Characterization of Enzymes written by Thomas E. Crowley and published by Academic Press. This book was released on 2014-01-11 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides students with a working knowledge of the fundamental and advanced techniques of experimental biochemistry. Included are instructions and experiments that involve purification and characterization of enzymes from various source materials, giving students excellent experience in kinetics analysis and data analysis. Additionally, this lab manual covers how to evaluate and effectively use scientific data. By focusing on the relationship between structure and function in enzymes, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual provides a strong research foundation for students enrolled in a biochemistry lab course by outlining how to evaluate and effectively use scientific data in addition to offering students a more hands-on approach with exercises that encourage them to think deeply about the content and to design their own experiments. Instructors will find this book useful because the modular nature of the lab exercises allows them to apply the exercises to any set of proteins and incorporate the exercises into their courses as they see fit, allowing for greater flexibility in the use of the material. Written in a logical, easy-to-understand manner, Experiments in the Purification and Characterization of Enzymes: A Laboratory Manual is an indispensable resource for both students and instructors in the fields of biochemistry, molecular biology, chemistry, pharmaceutical chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. Offers project lab formats for students that closely simulate original research projects Provides instructional guidance for students to design their own experiments Includes advanced analytical techniques Contains adaptable modular exercises that allow for the study proteins other than FNR, LuxG and LDH Includes access to a website with additional resources for instructors

Download or read book Studies on Purification and Characterization of an Autolytic Enzyme from Cell Walls of Bacillus Subtilis written by Willie Claiborne Brown and published by . This book was released on 1968 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this investigation was to purify and characterize the autolytic enzyme from cell walls of Bacillus subtilis 168. The crude enzyme was obtained by autolysis of purified cell walls in buffer at 37° C. Two purification methods were developed. The first involved fractional precipitation with ammonium sulfate. The enzyme activity was found in the 30-85% fraction. Purification by this method was 3.2 fold while the recovery was 35%. A second, more efficient, method was developed using ethanol as a precipitant in the presence of 0.1 M NaCl. Crude autolysates were precipitated with 75% cold ethanol. The precipitate was dissolved and exposed to 33% ethanol. This fraction was further purified by chromatography on Bio Gel A50m. Active fractions were pooled and concentrated. This scheme resulted in a purification of 14 fold and a yield of 31%. With heat-inactivated cell walls as substrate the partially purified autolytic enzyme was active at temperatures from 30°C to 62°C with maximum activity at 54°C. The pH optimum was broad (7-10); maximum activity occurred at pH 9-9.5. Divalent cations were required for activity. Activation occurred with Ba+, Ca++, Mg ++, and Mn ++. The reaction was inhibited by Fe++ and Cu++. Solutions of enzyme were stable for several hours at room temperature and for at least two months at -20°C. Activity was unaffected by freezing and thawing during this period. Lyophilization caused a 50% reduction in activity. No evidence for proteolytic activity was found. The partially purified enzyme contained 3% tightly bound organic phosphorus which was assumed to be in teichoic acid. This complex was not dissociated by several physical methods such as electrophoresis, ion-exchange chromatography, and gel filtration. These findings permitted tentative characterization of the enzyme as an acidic glycoprotein.

Download or read book Guide to Protein Purification written by Richard R Burgess and published by Academic Press. This book was released on 2009-11-03 with total page 915 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition’s publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines, including biochemistry, genetics, oncology, pharmacology, dermatology and immunology Assembles chapters on both common and less common relevant techniques Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process

Download or read book Biocatalysis for Practitioners written by Gonzalo de Gonzalo and published by John Wiley & Sons. This book was released on 2021-07-19 with total page 54 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.

Download or read book Undergraduate Research in the Sciences written by Sandra Laursen and published by Jossey-Bass. This book was released on 2010-06-10 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Undergraduate research (UR) is widely believed to enhance the learning experience of students in science, technology, engineering, and mathematics programs. This is the first comprehensive, practical, research-based book on undergraduate research. It addresses how the benefits to UR participants arise; compares the benefits of UR with other types of educational activities or experience; the long-term value of UR; and more. Intended to assist both existing and new UR practitioners with program design and evaluation needs, the book will also be useful to the wider community of academics, policy-makers, and funders of UR programs.

Download or read book Purification and Characterization of the Enzyme Responsible for Hydrolysis of Ethyl Acetate by Hansenula Anomala written by Sheril Dale Burton and published by . This book was released on 1961 with total page 47 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Maillard Reactions in Chemistry Food and Health written by T P Labuza and published by Elsevier. This book was released on 1998-01-01 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Maillard reaction was originally studied due to its importance in foods. Lately, it has been found to play a key role in many health-related issues. It is now associated with diabetes, ageing and cancer. The 5th International Symposium on The Maillard Reaction was held at the University of Minnesota, USA, in August 1993. This volume of conference proceedings presents recent research and discusses aspects of the chemistry, kinetics, technology and toxicology of this reaction.

Download or read book Microbial Enzymes and Biotechnology written by W.M. Fogarty and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biotechnology is now one of the major growth areas in science and engineering and within this broad discipline enzyme technology is one of the areas earmarked for special and significant developments. This publication is the second edition of Microbial Enzymes and Biotechnol ogy which was originally published in 1983. In this edition the editors have attempted to bring together accounts (by the relevant experts) of the current status of the major areas of enzyme technology and specifically those areas of actual and/or potential commercial importance. Although the use of microbial enzymes may not have expanded at quite the rate expected a decade ago, there is nevertheless intense activity and considerable interest in the whole area of enzyme technology. Microbial enzymes have been used in industry for many centuries although it is only comparatively recently that detailed knowledge relating to their nature, properties and function has become more evident. Developments in the 1960s gave a major thrust to the use of microbial enzymes in industry. The commercial success of alkaline proteases and amyloglucosidases formed a bed-rock for subsequent research and development in the area.

Download or read book Purification and Characterization of Proteolytic Enzymes from Bacteroides written by EARL MICHAEL LESK and published by . This book was released on 1969 with total page 70 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Xylanolytic Enzymes written by Pratima Bajpai and published by Academic Press. This book was released on 2014-02-03 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt: Xylanolytic Enzymes describes the enzyme structure and its interaction with plant cell walls, the properties and production of different enzymes and their application, and the knowledge gathered on the hydrolysis mechanism of hemicellulose. The knowledge gathered about the hydrolysis mechanism of the hemicelluloses, especially xylans, has greatly promoted the rapid application of these enzymes in new areas. Recently there has been much industrial interest in xylan and its hydrolytic enzymatic complex, as a supplement and for the manufacturing of food, drinks, textiles, pulps and paper, and ethanol; and in xylitol production as a fermentation substrate for the production of enzymes. This book describes xylan as a major component of plant hemicelluloses. Presents a thorough overview of all aspects of xylanolytic enzymes Gives up-to-date authoritative information and cites pertinent research Includes studies on xylanase regulation and synergistic action between multiple forms of xylanase

Download or read book Enzyme Assays written by Robert Eisenthal and published by Practical Approach. This book was released on 2002 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used tomeasure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters.Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gelelectrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening;statistical analysis of enzyme kinetic data; and the determination of active site concentration.This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.

Download or read book Chitin in Nature and Technology written by G.W. Gooday and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 562 pages. Available in PDF, EPUB and Kindle. Book excerpt: Exegi monumentum ael'e perennius. The monument I have built will last longer than bronze. Horace My previous book, "Chitin", (1977) was listed by the publisher, as a "key research book", among the most requested books by libraries. It received favorable comments from. each of the journals which reviewed it, Science, 198, 28 Oct. 1977, Physiological Entomology, 2(4), Dec. 1977, The Canadian Institute of Food Science and Technology Journal, April 1978, The Quarterly Review of Biology, 53:361, 1978, Oceanographic Abstracts, 15:182, 1979, Annales de Zoologie-Ecologie Animale, 11:127, 1979, and Enzyme & Microbial Technology, 2, 1980. The variety of these journals testifies to the interdisci plinary character of chitin studies. "Chitin" has really been a landmark, to use the definition given by Science, because it stimulated interest in the less known polysaccharides and in modified chitins, besides chitin itself, to the point that three International Conferences on Chitin / Chitosan were convened (Boston, U. S. A. 1977, Sapporo, Japan 1982 and Senigallia, Italy 1985). In convening the 3rd International Conference on Chitin / Chitosan (1-4 April 1985), one of the main objectives was the preparation of the present book. While the proceedings of the previous two Conferences were very valuable, they did not appear in any book catalogs and this severely Ii mi ted their distribution.

Download or read book Production Purification and Characterization of Thermostable Protease from Alkaliphilic and Thermophilic written by Seden Güracar and published by . This book was released on 2011 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteases are the hydrolase enzymes that catalyze the hydrolysis of the peptide bonds in the primary structure of proteins and peptids. They are used to cleave the proteins specifically to produce useful peptides in the processes. Proteases are present in a wide variety of living organisms and they also show different physicological, physicochemical, biological, chemical functions on the earth. They are the most important enzymes in the industry, accounting for 60% of the total enzyme scales in the world. The microorganisms that were previously isolated and characterized as a Bacillus sp. from Balçova Geotermal region in İzmir were used in the experiments. The aim of this study was to produce the protease enzyme from alkaliphilic and thermophilic Bacillus sp., purify and determine the properties of the enzyme with the characterization steps. When the screening studies and growth conditions were investigated, it was understood that the alkaliphilic and thermophilic Bacillus sp. produced extracellular protease enzyme. This extracellular protease enzyme was purified by ammonium sulphate precipitation and ion exchange chromatography chromatograpy. The yield and purification fold after purification of the enzyme were 33% and 1.41, respectively. In the characterization studies, the results indicated that the protease enzyme had highest activity at pH 8.0 and 55 C. The protease enzyme lost 20% of its activity at pH 4.0 and it lost 10% of its activity at pH 10.0. The protease enzyme at temperatures below 55 C lost 15% of its activity and also the protease enzyme at temperatures above 55 C lost 25% of its activity. The protease enzyme was stable at different pH values during 3 hours and at different temperature values during 6 hours. When compared the substrates, casein showed higher activity. The effect of organic solvents and surfactants on protease activity was investigated and the results indicated that the protease enzyme was stable in the presence of 10% of the organic solvents and 1% of the surfactants. PMSF and the protease inhibitor coctail decrease the activity of the protease.

Download or read book Principles of Enzyme Kinetics written by Athel Cornish-Bowden and published by Elsevier. This book was released on 2014-05-20 with total page 221 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles of Enzyme Kinetics discusses the principles of enzyme kinetics at an intermediate level. It is primarily written for first-year research students in enzyme kinetics. The book is composed of 10 chapters. Chapter 1 provides the basic principles of enzyme kinetics with a brief discussion of dimensional analysis. Subsequent chapters cover topics on the essential characteristics of steady-state kinetics, temperature dependence, methods for deriving steady-state rate equations, and control of enzyme activity. Integrated rate equations, and introductions to the study of fast reactions and the statistical aspects of enzyme kinetics are provided as well. Chemists and biochemists will find the book invaluable.

Download or read book Rational Design of Stable Protein Formulations written by John F. Carpenter and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recombinant proteins and polypeptides continue to be the most important class of biotechnology-derived agents in today's pharmaceutical industry. Over the past few years, our fundamental understanding of how proteins degrade and how stabilizing agents work has made it possible to approach formulation of protein pharmaceuticals from a much more rational point of view. This book describes the current level of understanding of protein instability and the strategies for stabilizing proteins under a variety of stressful conditions.

Download or read book Purification and Characterization of Bacterial Phospholipid Biosynthetic Enzymes written by Timothy Joseph Larson and published by . This book was released on 1978 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Handbook of Proteolytic Enzymes Volume 1 written by Alan J. Barrett and published by Elsevier. This book was released on 2012-12-02 with total page 1182 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Proteolytic Enzymes, Second Edition, Volume 1: Aspartic and Metallo Peptidases is a compilation of numerous progressive research studies on proteolytic enzymes. This edition is organized into two main sections encompassing 328 chapters. This handbook is organized around a system for the classification of peptidases, which is a hierarchical one built on the concepts of catalytic type, clan, family and peptidase. The concept of catalytic type of a peptidase depends upon the chemical nature of the groups responsible for catalysis. The recognized catalytic types are aspartic, cysteine, metallo, serine, threonine, and the unclassified enzymes, while clans and families are groups of homologous peptidases. Homology at the level of a family of peptidases is shown by statistically significant relationship in amino acid sequence to a representative member called the type example, or to another member of the family that has already been shown to be related to the type example. Each chapter discusses the history, activity, specificity, structural chemistry, preparation, and biological aspects of the enzyme. This book will prove useful to enzyme chemists and researchers.