Download or read book Engineering Of with Lipases written by F.X. Malcata and published by Springer Science & Business Media. This book was released on 1996-03-31 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: The innovative uses of lipases in a wide variety of organic syntheses and the modification of existing fats and oils have increased exponentially over the last five years, due to the increasing availability of lipases from (genetically engineered) microbial sources, coupled with their special capacity to act as catalysts at hydrophilic/hydrophobic interfaces. As a result of the structural characterisation performed during the same period, applications of these lipases can now be developed in a much more rational way. Engineering of/with Lipases presents two major topics: the design and production of lipases with desired, preselected properties, and the use of lipases for desired applications. Audience: Doctoral and post-doctoral crystallographers, biochemists, geneticists and enzyme kineticists. Food, chemical and biochemical engineers. The former will learn about the practical aims and constraints associated with industrial applications of lipases, enabling them to design lipases for specific purposes. The latter will learn how to take advantage of the structural knowledge of lipases and their metabolic genesis to better design media, processes and products in terms of biochemical and technical feasibility.
Download or read book Lipases written by Lilia Alberghina and published by Wiley-VCH. This book was released on 1991 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipases are of high practical relevance. They exhibit activity only at the water-lipid interphase and can thus be utilized for various industrial applications such as detergents and chiral synthesis. Up to now, however, progress in industrial use has been hampered by the lack of fundamental knowledge about lipase structure and function. With the information this GBF monograph provides, scientists will finally be able to develop and exploit lipases in their own work. The 70 articles in the book, written by scientists from 16 nations (notably from Europe, USA and Japan), are grouped into four sections: * 3-Dimensional Structure * Mechanism of Action * Genetic Engineering * General Characterization and Purification This unique and invaluable documentation is the result of a CEC-GBF Workshop (Braunschweig, Germany, September 1990) held within the framework of the BRIDGE EEC project.
Download or read book Lipases written by L. Alberghina and published by Wiley-Blackwell. This book was released on 1991-06-14 with total page 441 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipases are of high practical relevance. They exhibit activity only at the water-lipid interphase and can thus be utilized for various industrial applications such as detergents and chiral synthesis. Up to now, however, progress in industrial use has been hampered by the lack of fundamental knowledge about lipase structure and function. With the information this GBF monograph provides, scientists will finally be able to develop and exploit lipases in their own work. The 70 articles in the book, written by scientists from 16 nations (notably from Europe, USA and Japan), are grouped into four sections: * 3-Dimensional Structure * Mechanism of Action * Genetic Engineering * General Characterization and Purification This unique and invaluable documentation is the result of a CEC-GBF Workshop (Braunschweig, Germany, September 1990) held within the framework of the BRIDGE EEC project.
Download or read book Lipases and Phospholipases written by Georgina Sandoval and published by Humana. This book was released on 2019-12-10 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases. After an overview, the book delves into activity screening and expression, optimization of the biocatalyst production and performances, and applications of lipases, phospholipases, and esterases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Lipases and Phospholipases: Methods and Protocols, Second Editionserves as an updated reference book for the large scientific community, both seasoned and novice, working with lipases, phospholipases, and related enzymes.
Download or read book Lipases written by Hannes Broeken and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this collection, the authors discuss endothelial lipase (EL), a recently identified member of the lipase gene family which exhibits a high degree of sequence similarity to lipoprotein lipase and hepatic lipase. The opening chapter summarizes the recent findings with regard to the molecular structure and biological mechanisms of EL, factors affecting the EL expression and activity as well as its role in some inflammatory disorders. Additionally, enzyme lipase is discussed; specifically its wide range of applications in the food, cosmetic, pharmaceutical as well as oleochemical industries. The capability of enzyme lipase to catalyse reactions with higher efficiency and stability, their chemo-, regio- and enantioselectivity properties, as well as their eco-friendly characteristics resulted in their high demand worldwide. Following this, the use of enzyme immobilization as an efficient strategy to overcome certain drawbacks is analyzed. Immobilization of lipases enhances properties such as thermostability and activity, facilitates separation of products, and provides more flexibility with enzyme/substrate contact by using various reactor configurations. This chapter will also highlight benefits of immobilization in lipase stabilization with respect to other employed methods such as chemical modification and recombinant DNA technology. In the concluding study, three commercially available lipases, namely Lipase PS-CI and Lipase PS-DI from Pseudomonas cepacia (non-specific lipase), and Lipozyme RM-IM from Rhizomucor miehei (1,3-regioselective lipase) were screened for their effect on catalyzing the acidolysis of tricaprylin with ALA. The effect of substrate molar ratio, temperature and time of reaction on incorporation of ALA were investigated and optimized by response surface methodology. The study reveals the potential of lipase-catalyzed acidolysis of palm oil with ALA as a suitable alternative to improve the nutritional properties and health-beneficial effects of edible oils.
Download or read book Genetic Engineering and Directed Evolution of Staphylococcal Lipases written by Muriel Daniëla van Kampen and published by . This book was released on 2000 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Lipases and Phospholipases written by Georgina Sandoval and published by Humana Press. This book was released on 2016-05-01 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book details fundamental issues and new applications of lipases and phospholipases. It provides step-by-step detail essential for reproducible results and contains key notes and implementation advice from the experts."
Download or read book Lipases and Phospholipases in Drug Development written by Günter Müller and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipases and Phospholipases are key control elements in mammalian metabolism. They share many common features that set them apart from other metabolic enzyme classes, most importantly their association with biological membranes. Their potential as drug targets for the treatment of metabolic diseases is widely recognized, and the first lipase inhibitor drugs have been successfully introduced. Providing drug developers with a firm foundation for lipase-centered drug design, the editors of this volume have assembled experts from different scientific disciplines to create a comprehensive handbook for all pharmaceutical chemists, biochemists and physiologists working with lipases. The authors examine fundamental aspects of lipase function in vitro and in vivo, explaining how this knowledge may be used to develop lipase assays. They also treat the physiological roles of lipases in normal and disordered metabolism, as well as strategies to target lipases for the treatment of diabetes, obesity and related disorders. Additional topics include the application of phospholipases for liposome-based drug delivery and their use as diagnostic tools.
Download or read book Hydrolases in Organic Synthesis written by Uwe Theo Bornscheuer and published by John Wiley & Sons. This book was released on 2006-05-12 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: From reviews to the first edtion: "Bornscheuer and Kazlauskas have set out, and succeeded, in producing a definitive manual on hydrolytic enzymes (especially lipases, esterases, and proteases) for organic chemists. This is quite simply the best book of its type and can be unreservedly recommended to organic chemists who have an interest in using hydrolytic enzymes in synthesis." (Nicholas J. Turner, University of Edinburgh) "The book is an indispensable source of information on the use of hydrolases in organic synthesis. The subject matter is very well set out, and the chapters are clearly written and presented from a critical viewpoint. Bornscheuer and Kazlauskas have succeeded admirably in describing the capabilities and limitations of the use of hydrolytic enzymes and in critically evaluating them. No library should be without the book." (Fritz Theil, WITEGA Angewandte Werkstoff-Forschung GmbH, Berlin) The second edition of this extremely successful and well-proven book presents recent developments in the use of hydrolases for organic synthesis, reflecting in particular the enormous progress made in enzyme discovery and optimization with a new chapter on "Protein Sources and Optimization of Biocatalyst Performance". The renowned authors survey the stereoselective reactions of hydrolases, especially lipases, esterases and proteases, giving researchers an overview of what has worked in the past so that they can judge how to solve their own synthetic problems. In total, the book contains over one thousand chemical structures, rounded off by some 1,800 invaluable references.
Download or read book Lipase written by B.K. Konwar and published by CRC Press. This book was released on 2018-05-23 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbial lipases are industrially important and have gained attention due to their stability, selectivity, and broad substrate specificity. Lipases are used as medicine, and they also aid in indigestion, heartburn, allergy to gluten in wheat products (celiac disease), Crohn’s disease, and cystic fibrosis. This volume considers the industrial demand for new sources of lipases with different catalytic characteristics that stimulate the isolation, growth, and development of new microbial strains. The volume narrates the challenging metagenomic approach with the isolation of the lipase gene, its cloning into Escherichia coli, culture of the recombinant bacteria, and extraction and assessment of the lipase enzyme. Lipase-producing bacteria are available in different habitats, such as industrial wastes, vegetable oil processing factories, dairy plants, and soils contaminated with oil and oil seeds, among others. This volume is the effort of the authors to document the scientific findings carried out over the last eight years in the area of un-culturable soil microorganisms. The book presents the physic-chemical features of lipases and their specific applications in different commercial industries. The in-depth study looks at metagenomics for lipases from all angles and provides a truly informative resource. It describes the biochemical characterization of lipase enzymes with the high activity in the presence of 1% tributyrin. A wide review has been presented in the book on lipase enzymes purified from a large collection of microbes present in soil, seawater, waste-dumping sites, animal systems (including human beings), and the atmosphere. Stability of enzymes over changing environments of the industry is indeed a big issue, and the book deals at length with the changing temperatures and pH and metal ion concentrations.
Download or read book Enzymes in Industry written by Wolfgang Aehle and published by John Wiley & Sons. This book was released on 2006-05-12 with total page 508 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading experts from all over the world present an overview of the use of enzymes in industry for: - the production of bulk products, such as glucose, or fructose - food processing and food analysis - laundry and automatic dishwashing detergents - the textile, pulp and paper and animal feed industries - clinical diagnosis and therapy - genetic engineering. The book also covers identification methods of new enzymes and the optimization of known ones, as well as the regulatory aspects for their use in industrial applications. Up to date and wide in scope, this is a chance for non-specialists to acquaint themselves with this rapidly growing field. '...The quality...is so great that there is no hesitation in recommending it as ideal reading for any student requiring an introduction to enzymes. ...Enzymes in Industry - should command a place in any library, industrial or academic, where it will be frequently used.' The Genetic Engineer and Biotechnologist
Download or read book Lipase and Phospholipase Protocols written by Mark Doolittle and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: The lipases and phospholipases represent a diverse group of enzymes that are expressed in animals, plants, fungi, and bacteria. Their ubiquitous distribution among all species is a testament to the essential roles played by these enzymes in lipid storage, mobilization and metabolism, membrane homeostasis and remodeling, endocrine and immune functions, and signal tra- duction. In humans, lipases and phospholipases are also thought to contribute to complex diseases, such as atherosclerosis, obesity, arthritis, and cancer, as well as to single gene defects, such as Wolman's disease and Type I hyperlipoproteinemia. Enzymatically, the lipases are unique, since they hydrolyze substrates that are either insoluble, or only partly soluble, in aq- ous solvents; thus, enzyme catalysis takes place at a lipid-water interface. The interface comprises at least two, and often more, discrete bulk and s- face phases, in which the enzyme, substrate, and products oflipolysis disperse among these phases based on their physical properties. Furthermore, the d- tribution of these components changes continuously as lipolysis proceeds. Thus, the lipases and phospholipases are fundamentally different from any other enzyme because of the physical complexity of the environment in which catalysis occurs.
Download or read book PCR Technology written by Henry Erlich and published by Springer. This book was released on 2015-12-31 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.
Download or read book Thermophilic Moulds in Biotechnology written by B.N. Johri and published by Springer Science & Business Media. This book was released on 1999-09-30 with total page 376 pages. Available in PDF, EPUB and Kindle. Book excerpt: All important aspects of thermophilic moulds such as systematics, ecology, physiology and biochemistry, production of extracellular and intracellular enzymes, their role in spoilage of stores products and solid and liquid waste management, and general and molecular genetics have been dealt with comprehensively by experts in this book which covers progress in the field over the last 30 years since the seminal book Thermophilic Fungi published by Cooney and Emerson in 1964. The experts have reviewed extensive literature on all aspects of thermophilic moulds in a very comprehensive manner. This book will be useful for graduates as well as post-graduate students of life sciences, mycology, microbiology and biotechnology, and as a reference book for researchers.
Download or read book Protein Engineering For Industrial Biotechnology written by Lilia Alberghina and published by CRC Press. This book was released on 2000-01-10 with total page 393 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein engineering has proved to be one of the more fruitful technological approaches in biotechnology, being both very powerful and able to generate valuable intellectual property. This book aims to present examples in which the application of protein engineering has successfully solved problems arising in industrial biotechnology. There is a sec
Download or read book Production and optimization of extracellular lipase by Bacillus subtilis using submerged fermentation written by Prem Jose Vazhacharickal and published by Prem Jose. This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipase is an extracellular enzyme in food industry and is produced by different microbial and fungal sources by submerged and solid state fermentation process. In the present study, Bacillus subtilis was employed for the production of lipase in peptone broth with olive oil as inducer. Maximum lipase production was observed at 96 hours of incubation and NH4Cl (40.75 U/ml) as salt and peptone (24U/ml) as nitrogen source and olive oil (24 U/ml) as inducer. The results of the present study demonstrate that Bacillus subtilis is ideal for extracellular production at industrial level.
Download or read book Engineering Sciences and Technologies Researches written by Pınar BERKAN and published by Livre de Lyon. This book was released on 2023-12-24 with total page 181 pages. Available in PDF, EPUB and Kindle. Book excerpt: Engineering Sciences and Technologies Researches
