Download or read book Development Optimization and Characterization of a Surface Enhanced Raman Spectroscopic Method for Detection of Dipicolinic Acid written by Joy D. Guingab and published by . This book was released on 2007 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: ABSTRACT: Surface Enhanced Raman Spectroscopy (SERS) has been explored as a tool to study biological agents. The anthrax distribution in the US postal in October 2001 intensified the need for a rapid and accurate detection of bacterial spores. Dipicolinic acid (DPA) is a unique component of bacterial spores and has been used as a signature molecule for detection schemes. The feasibility of using SERS as a detection technique for bacterial spores is evaluated in this research.
Download or read book Surface Enhanced Raman Spectroscopy written by Marek Prochazka and published by Springer. This book was released on 2015-12-12 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives an overview of recent developments in RS and SERS for sensing and biosensing considering also limitations, possibilities and prospects of this technique. Raman scattering (RS) is a widely used vibrational technique providing highly specific molecular spectral patterns. A severe limitation for the application of this spectroscopic technique lies in the low cross section of RS. Surface-enhanced Raman scattering (SERS) spectroscopy overcomes this problem by 6-11 orders of magnitude enhancement compared with the standard RS for molecules in the close vicinity of certain rough metal surfaces. Thus, SERS combines molecular fingerprint specificity with potential single-molecule sensitivity. Due to the recent development of new SERS-active substrates, labeling and derivatization chemistry as well as new instrumentations, SERS became a very promising tool for many varied applications, including bioanalytical studies and sensing. Both intrinsic and extrinsic SERS biosensing schemes have been employed to detect and identify small molecules, nucleic acids and proteins, and also for cellular and in vivo sensing.
Download or read book Surface enhanced Raman Spectroscopy written by Vinay Bhardwaj and published by . This book was released on 2019 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last decade, we have seen spurring growth in academic and industrial interest in Raman spectroscopy and its modifications, in particular, Surface-Enhanced Raman Spectroscopy (SERS). This is evident by the number of SERS publications in the last decade, from 580 in 2005 to almost 2000 in 2014. There are now companies worldwide that are dedicated to manufacturing SERS substrate, sensors, and other SERS-based products. The miniaturization of Raman instrumentation from bulky and expensive bench-top designs to inexpensive handheld spectrometers has allowed SERS to be employed as an in situ detection technique. Some examples of the industrial success of Raman and SERS are rapid raw material identification (RMID), Process Analytical Technology (PAT) and drug screening in pharma and biotech industries, continuous surveillance of food, water, and environmental safety, and the diagnosis and continuous monitoring of diseases like cancer, diabetes, etc. This progress in translating Raman and SERS techniques into commercial use and commercial products is possible because of the close collaboration between scientists and engineers. However, the weak signals of Raman spectroscopy is an inherent limitation of the spontaneous Raman spectroscopy technique. Therefore, several modifications have been made, including but not limited to, surface-enhanced Raman spectroscopy (SERS), spatially offset Raman spectroscopy (SORS), transmission Raman spectroscopy (TRS), coherent anti-Stokes Raman spectroscopy (CARS), and tip-enhanced Raman spectroscopy (TERS). Spontaneous Raman spectroscopy has become a one-of-a kind, portable, and affordable technology for in-situ detection and continuous monitoring in resource-limited-settings. The primary focus of this textbook is to try to cover the fundamentals in SERS methods of preparing substrates and sensors, and efficient spectral analysis approaches like chemometrics for diverse applications, listed in the paragraph above. However, with a goal to give a true picture to the audience when assessing the scope of spontaneous Raman and Surface-enhanced Raman for specific applications, we have briefly introduced industrially successful examples of other types of Raman spectroscopy techniques, such as SORS, TRS, and CARS.
Download or read book Surface Enhanced Raman Spectroscopy for the Detection of Oncometabolites written by Daniel Hans Zimmermann and published by . This book was released on 2021 with total page 55 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mithilfe von Raman-Spektroskopie ist es möglich, ortsaufgelöste Informationen über die molekulare Zusammensetzung von biologischen Proben zu gewinnen. Durch metallische Nanostrukturen kann die von Natur aus schwache Raman-Streuung enorm verstärkt werden (SERS). In dieser Arbeit wurde die Anwendung von SERS zum Nachweis von Onkometaboliten in chemotherapeutisch behandelten und unbehandelten Hodgkin-Lymphomzellen untersucht. Verschiedene SERS-Substrate wurden verglichen und eine optimierte Raman-methode für Hodgkin-Lymphomzellen wurde entwickelt. Zur gezielten Differenzierung und verbesserten Interpretation der erhaltenen Raman-Spektren wurde eine automatisierte Auswertung basierend auf Hauptkomponentenanalyse (PCA) und linearer Diskriminanzanalyse (LDA) entwickelt. Behandelte und unbehandelte Zellen konnten durch die optimierte Auswertung mit hoher Genauigkeit voneinander unter-schieden werden. Die biologischen Grundlagen dieser Unterscheidung wurden durch Bandenzuordnung sowie GC-MS-Referenzanalytik ermittelt. *****Using Raman spectroscopy, it is possible to gain spatially resolved information about the molecular composition of biological samples. The inherently weak Raman scattering can greatly enhanced through the addition of metallic nanostructures (SERS). In this thesis, the application of SERS for the detection of oncometabolite in chemotherapeutically treated and untreated Hodgkin-Lymphoma cells was investigated. Various SERS sub-strates were compared, and an optimized Raman method for Hodgkin-Lymphoma cells was developed. For targeted differentiation and improved interpretation of the resulting Raman spectra, an automated data analysis workflow was developed based on principal component analysis (PCA) and linear discriminant analysis (LDA). Treated and untreated cells were differentiated with high accuracy using this workflow. The biological basis of this differentiation was determined through band assignment as well as GC-MS refer-ence analysis.
Download or read book Raman Spectroscopy for Nanomaterials Characterization written by Challa S. S. R. Kumar and published by Springer Science & Business Media. This book was released on 2012-03-02 with total page 659 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook gives a comprehensive overview about Raman spectroscopy for the characterization of nanomaterials. It is the first volume of a 40-volume series on nanoscience and nanotechnology, edited by the renowned scientist Challa S.S.R. Kumar.
Download or read book Surface Enhanced Raman Spectroscopy for Biophysical Applications written by Claudia Fasolato and published by Springer. This book was released on 2018-12-05 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book explores the phenomenon of surface-enhanced Raman scattering (SERS), the huge amplification of Raman signal from molecules in the proximity of a metallic nanostructured surface, allowing readers to gain an in-depth understanding of the mechanisms affecting the spectroscopic response of SERS-active systems for effective applications. SERS spectroscopy is an ultrasensitive analytical technique with great potential for applications in the field of biophysics and nanomedicine. As examples, the author presents the design of nanocolloid-based SERS-active substrates for molecular sensing and of a folate-based SERS-active nanosensor capable of selectively interacting with cancer cells, enabling cancer diagnostics and therapy at the single-cell level. The author also suggests novel paths for the systematization of the SERS nanosystem design and experimental protocols to maximize sensitivity and reproducibility, which is essential when real-world biomedical applications are the goal of the study. With a combined approach, both fundamental and applied, and a detailed analysis of the state of the art, this book provides a valuable overview both for students new to SERS spectroscopy and for experts in the field.
Download or read book Surface Enhanced Raman Spectroscopy with High Spatial Resolution written by T. R. Huser and published by . This book was released on 2003 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The identification of individual molecules and the determination of how these interact with their local environment are critical steps toward a better understanding of complex organic systems. Optical detection techniques have always played a key role in the nondestructive and noninvasive analysis of complex materials. Until recently, however, optical microscopy has lacked the sensitivity to study processes on the molecular scale. This has changed with the recent development of new schemes that limit the optical detection volume, and the advent of new, highly quantum-efficient photon detectors. These inventions have enabled researchers to optically probe biomolecular processes at the single molecule level by observing the fluorescence of specific marker molecules. The requirement to specifically label biomolecules and the fact, that fluorescence emission is prone to photodecomposition of the marker molecules, however, have limited this approach to a few, well-characterized case studies. Raman scattering, is one of few optical techniques that can identify atomic species and in addition determine their chemical bonds by observing their distinct vibrational fingerprints; but it is orders of magnitude weaker than fluorescence. In this project, we have developed new optical probes that allow for the non-destructive characterization and identification of organic and inorganic matter at the single molecule level by surface-enhanced Raman spectroscopy. Our approach combines confocal Raman microscopy with surface-enhanced Raman spectroscopy (SERS) generated by coating scanning probe microscope (SPM) tips with thin (30-40 nm) gold and silver films. The scanning SERS probe generates an image of the physical structure of a sample together with detailed chemical information about its composition. In a complementary approach, we have also used gold or silver nanoparticles to generate SER spectra from single molecules adsorbed to these particles. This project has led to the development of a new capability at LLNL, i.e. the field of optical single molecule detection.
Download or read book Surface Enhanced Raman Spectroscopy written by Sebastian Schlücker and published by John Wiley & Sons. This book was released on 2011-09-19 with total page 97 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covering everything from the basic theoretical and practical knowledge to new exciting developments in the field with a focus on analytical and life science applications, this monograph shows how to apply surface-enhanced Raman scattering (SERS) for solving real world problems. From the contents: * Theory and practice of SERS * Analytical applications * SERS combined with other analytical techniques * Biophysical applications * Life science applications including various microscopies Aimed at analytical, surface and medicinal chemists, spectroscopists, biophysicists and materials scientists. Includes a Foreword by the renowned Raman spectroscopist Professor Wolfgang Kiefer, the former Editor-in-Chief of the Journal of Raman Spectroscopy.
Download or read book Raman Spectroscopy written by Gustavo Morari Do Nascimento and published by BoD – Books on Demand. This book was released on 2018-04-18 with total page 326 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives a wide overview of the state-of-the-art applications of Raman spectroscopy in characterization of materials and biomaterials. The Raman signal is intrinsically smaller than other vibrational techniques; however, mainly through intensification processes, such as resonance Raman (RR) and surface-enhanced Raman spectroscopy (SERS), the Raman cross section can be strongly amplified. Thoroughly in these signal amplifications, the study of a diversity of chemical systems and the use of Raman technique for in situ and in vivo measurements is possible. The main goal of this book is to open up to an extended audience the possibilities of uses of Raman spectroscopy. In fact, this collective work will be beneficial to students, teachers, and researchers of many areas who are interested to expand their knowledge about Raman spectroscopy applied to nanotechnology, biotechnology, environmental science, inorganic chemistry, and health sciences.
Download or read book Development of Unenhanced External Reflection Raman Spectroscopy and Surface enhanced Raman Scattering SERS Methods for Routine Structural Characterization of Biophysical Molecular Monolayers written by Chad Livingston Leverette and published by . This book was released on 2000 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Developing Robust Figures of Merit for Spectroscopic Characterization of Sphere Masks in Sensors Made by Nanosphere Lithography for Detection of a Cyanide Metabolite Via Surface Enhanced Raman Spectroscopy written by Deepthika De Silva and published by . This book was released on 2013 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development of Functional Materials for Surface enhanced Raman Spectroscopy Analysis written by Huanyu Chi and published by . This book was released on 2020 with total page 95 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Silver Mirror Substrate and Rolling Method for Improved Surface enhanced Raman Spesctroscopic Analysis in Food written by Yanqi Qu and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Surface-enhanced Raman spectroscopy, short for SERS, is an emerging technology with great potential in food analysis due to rapid detection, high sensitivity, portable instrumentation, and simple sample preparation. However, it is always a bottleneck to obtain reproducible SERS measurements in real analytical cases due to the complicity of food systems and inhomogeneous aggregation of colloidal nanoparticles. To improve its performance for practical applications in food analysis, efforts have been made in improving the reproducibility, enhancing the selectivity and reducing the matrix interference to the analyte. Herein, a self-assembly silver nanoparticles mirror substrate was fabricated to improve the and the quantitative ability and effectiveness of sample preparation for different applications in food analysis, including pesticides detection in beverages, chemical profiling of red wines, and headspace analysis of garlic. The AgNPs mirror was fabricated using the interface between polar and non-polar solvents and showed a uniform arrangement of nanoparticles under the microscope. It demonstrated a great reliability for the detection of a pesticide fonofos in beverages (i.e., apple juice and green tea). AgNPs mirror can also be in situ fabricated in red wines to generate a comprehensive spectrum constituted by signals of five wine phytochemicals, which provided a great potential of SERS in the differentiation, authentication, and quality/safety control of red wines. AgNPs mirror also showed great potential in the headspace characterization of aromatic compounds from Allium species plants. Additionally, a facile rolling method was developed to enrich analyte (i.e., chlordane pesticide) and to amplify its weak SERS activity and a mathematic model was generated and successfully quantified the chlordane in a complicated crude oil sample with a very good recovery. Overall, AgNPs mirror and the rolling method overcame the reproducibility and sensitivity problems for SERS in several challenging food matrices. With these improvements, SERS can be much more reliable for analytical applications and its range of targets can be widely expanded.
Download or read book Surface Enhanced Raman Spectroscopy SERS for Characterization of Particles in Solution written by Steven M. Asiala and published by . This book was released on 2014 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development of Surface Enhanced Raman Spectroscopic Silicon Substrate Microphobes for the Investigation of Subcutaneous Lipid Chemistry written by Millicent Kaye Weldon and published by . This book was released on 1999 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Surface And Tip Enhanced Raman Spectroscopy for Catalysis written by Bert M. Weckhuysen and published by . This book was released on 2021-12-07 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Beginning with a look at the theory behind the techniques this book provides a practical and hands-on description of the various methods of Surface- and Tip-Enhanced Raman Spectroscopy.
Download or read book Surface Enhanced Raman Spectroscopy for Interfacial Characterization written by Steven George Schultz and published by . This book was released on 1981 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
