Download or read book Development of Novel Microfluidic Platforms for Neural Stem Cell Research written by Bonggeun Chung and published by . This book was released on 2007 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: To make novel microfluidic devices integrated with multiple functions, we have also developed a microfluidic multi-injector (MMI) that can generate temporal and spatial concentration gradients. MMI consists of fluidic channels and control channels with pneumatically actuated on-chip barrier valves. Repetitive actuations of on-chip valves control pulsatile release of solution that establishes microscopic chemical gradients. The development of novel gradient-generating microfluidic platforms will help in advancing our understanding of brain development and provide a versatile tool with basic and applied studies in stem cell biology.
Download or read book Stem Cells written by Michal K. Stachowiak and published by World Scientific. This book was released on 2012 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fast-moving and ever-changing, stem cell science and research presents ongoing ethical and legal challenges in many countries. Each development and innovation throws up new challenges. This is the case even where new developments initially seem to solve old dilemmas. Sometimes it becomes evident that new science does not in fact solve old problems and, for that reason, the ethical issues remain. In recognition of this, this book presents innovative and creative analyses of a range of ethical and legal challenges raised by stem cell research and its potential and actual application. The editors.
Download or read book Stem Cell Engineering written by Gerhard M. Artmann and published by Springer Science & Business Media. This book was released on 2010-10-29 with total page 566 pages. Available in PDF, EPUB and Kindle. Book excerpt: The potential of stem cells for healing and disease prevention in all fields of medicine is tremendous and has revolutionized the high-tech biomedical research. In this book, many of the most prominent researchers discuss the challenging topics of stem cell engineering, for example: Ethical issues of stem cell research; technological challenges, stem cell growth and differentiation, therapeutic applications, bioreactors and bioprocesses, high throughput and microfluidic screening platforms, stem cell identification and sorting, intercellular signaling and engineered niches, novel approaches for embryonic and adult stem cell growth and differentiation, stem cells and drug discovery, screening platforms. Stem Cell Engineering offers valuable background and reference for both the public and professionals including industrial staffers, faculty, researchers, engineers, students and scientific journalists.
Download or read book Microfluidic Cell Culture Systems written by Jeffrey T Borenstein and published by Elsevier. This book was released on 2018-09-12 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: Techniques for microfabricating intricate microfluidic structures that mimic the microenvironment of tissues and organs, combined with the development of biomaterials with carefully engineered surface properties, have enabled new paradigms in and cell culture-based models for human diseases. The dimensions of surface features and fluidic channels made accessible by these techniques are well-suited to the size scale of biological cells. Microfluidic Cell Culture Systems applies design and experimental techniques used in in microfluidics, and cell culture technologies to organ-on-chip systems. This book is intended to serve as a professional reference, providing a practical guide to design and fabrication of microfluidic systems and biomaterials for use in cell culture systems and human organ models. The book covers topics ranging from academic first principles of microfluidic design, to clinical translation strategies for cell culture protocols. The goal is to help professionals coming from an engineering background to adapt their expertise for use in cell culture and organ models applications, and likewise to help biologists to design and employ microfluidic technologies in their cell culture systems. This 2nd edition contains new material that strengthens the focus on in vitro models useful for drug discovery and development. One new chapter reviews liver organ models from an industry perspective, while others cover new technologies for scaling these models and for multi-organ systems. Other new chapters highlight the development of organ models and systems for specific applications in disease modeling and drug safety. Previous chapters have been revised to reflect the latest advances. - Provides design and operation methodology for microfluidic and microfabricated materials and devices for organ-on-chip disease and safety models. This is a rapidly expanding field that will continue to grow along with advances in cell biology and microfluidics technologies. - Comprehensively covers strategies and techniques ranging from academic first principles to industrial scale-up approaches. Readers will gain insight into cell-material interactions, microfluidic flow, and design principles. - Offers three fundamental types of information: 1) design principles, 2) operation techniques, and 3) background information/perspectives. The book is carefully designed to strike a balance between these three areas, so it will be of use to a broad range of readers with different technical interests and educational levels.
Download or read book Stem Cell Engineering written by Robert M. Nerem and published by Springer. This book was released on 2014-06-12 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes a global assessment of stem cell engineering research, achieved through site visits by a panel of experts to leading institutes, followed by dedicated workshops. The assessment made clear that engineers and the engineering approach with its quantitative, system-based thinking can contribute much to the progress of stem cell research and development. The increased need for complex computational models and new, innovative technologies, such as high-throughput screening techniques, organ-on-a-chip models and in vitro tumor models require an increasing involvement of engineers and physical scientists. Additionally, this book will show that although the US is still in a leadership position in stem cell engineering, Asian countries such as Japan, China and Korea, as well as European countries like the UK, Germany, Sweden and the Netherlands are rapidly expanding their investments in the field. Strategic partnerships between countries could lead to major advances of the field and scalable expansion and differentiation of stem cells. This study was funded by the National Science Foundation (NSF), the National Institutes of Health (NIH) and the National Institute of Standards and Technology (NIST).
Download or read book Development of Microfluidic Cell Culture Platforms for Investigating Cellular Phenomena written by Bryson McKinley Brewer and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Emerging Technology Platforms for Stem Cells written by Uma Lakshmipathy and published by Wiley. This book was released on 2009-04-06 with total page 438 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on practical applications for using adult and embryonic stem cells in the pharmaceutical development process. It emphasizes new technologies to help overcome the bottlenecks in developing stem cells as therapeutic agents. A key reference for professionals working in stem cell science, it presents the general principles and methodologies in stem cell research and covers topics such as derivitization and characterization of stem cells, stem cell culture and maintenance, stem cell engineering, applications of high-throughput screening, and stem cell genetic modification with their use for drug delivery.
Download or read book Development of a Novel Microfluidic Platform to Study T Cell Signaling written by Shannon L. Faley and published by . This book was released on 2007 with total page 109 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Stem Cell Bioprocessing written by Tiago G. Fernandes and published by Elsevier. This book was released on 2013-11-15 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem cell bioprocessing describes the main large-scale bioprocessing strategies for both stem cell culture and purification, envisaging the application of these cells for regenerative medicine and drug screening. Bioreactor configurations are described, including their applications for stem cell expansion, and stem cell separation techniques such as isolation and purification are discussed. Basic definitions are provided concerning the different types of stem cells, from adult stem cells to the more recent induced pluripotent stem cells. The main characteristics of these different stem cell types are described, alongside the molecular mechanisms underlying their self-renewal and differentiation. The book also focuses on methodologies currently used for in vitro stem cell culture under static conditions, including the challenge of xeno-free culture conditions, as well as culture parameters that influence stem cell culture. Approaches for both stem cell culture and separation in micro-scale conditions are presented, including the use of cellular microarrays for high-throughput screening of the effect of both soluble and extracellular matrix molecules. A further section is dedicated to application of stem cells for regenerative medicine. - Maintains a unique focus on both the basic stem cell biology concepts, and their translation to large-scale bioprocessing approaches - Envisages the use of stem cells in regenerative medicine and drug screening applications - Discusses the application of microscale techniques as a tool to perform basic stem cell biology studies
Download or read book Microfluidic Bio sensor Development for Clinical Applications written by Kyle Matthew Wellmerling and published by . This book was released on 2021 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidic devices have revolutionized the the field of cell biology. As microfluidicdevices became affordable and mainstream, they began to serve as miniature labs for detecting and studying biological phenomenon. We develop microfluidic devices for two different biological applications in this thesis. In Chapter 2, we develop a microfluidic device for removal of rare, teratoma-forming cells from human-induced pluripotent stem cell-derived neural progenitors (hiPSC-NPCs). Though generalizable to other cell types, we begin by characterizing the differentiation process of human-induced pluripotent stem cells (hiPSCs) into neural progenitor cells (NPCs) based off residual pluripotency signature and investigate device performance by removing residual teratoma-forming cells. In Chapter 3, we develop a platform for mimicking the early stages of Lyme transmission and apply our device towards detecting Borellia burgdorferi, the causative agent of Lyme Disease. Early research on Lyme disease suggested a model in which the outer surface protein OspA is expressed by Borrelia in the tick, while outer surface protein OspC is expressed in the mammalian host. We first develop a culture system for recreating this dynamic change in surface protein expression, and then demonstrate our device is capable of capturing and detecting Borellia burgdorferi. Teratoma formation remains a safety concern in therapeutic cells derived from hiPSCs. Residual Teratoma forming cells are present in small numbers in differentiated hiPSC cultures and yet are of significant roadblock to the manufacturing and clinical translation of stem cell therapies. Rare cells are often difficult to remove with standard flow cytometry or magnetic bead sorting techniques. Here, we first characterized time-dependent expression of a teratoma marker, stage-specific embryonic antigen 5 (SSEA-5), which binds the H type-1 glycan during neural differentiation of hiPSCs. The fraction of cells SSEA-5+ remained high at 97% on day 3, dropped to 70% on day 4, 40% by day 6, and down to 1% on day 12 of differentiation, indicating successful differentiation. We engineered a microfluidic geometrically enhanced differential immunocapture (GEDI) technology to remove SSEA-5+ rare cells from hiPSC-derived neural progenitor cells (hiPSC-NPCs). The GEDI chip removed more than 95% of teratoma-forming cells and presents a facile tool to potentially functionalize with multiple antibodies and robustly enhance hiPSC-derived cell population safety prior to therapeutic transplantation. The approach is potentially amenable to generate a wide variety of high-quality therapeutic cells and can be integrated within the pipeline of cell manufacturing to improve patient safety and reduce the cost of manufacturing through early removal of undesirable cell types. Lyme Disease is a multisystem infectious disease caused by the Borellia burgdorferi complex, and is a growing threat to public health. Approximately 476,000 people are infected with Lyme in the United States each year. Although Lyme is readily treated with antibiotics when detected early, early detection remains difficult. Current testing remains difficult because the standard 2-tiered ELISA assay indirectly detects Lyme via measurement of a host immune response, which suffers from an inherent time-lag in host antibody production. A direct test for Lyme Disease would overcome these inherent limitations. To this end we report on the first microfluidic immunocapture device for Lyme Disease. We engineered a geometrically enhanced differential immunocapture (GEDI) technology to capture whole-organism Borrelia for direct on-chip detection. This approach is potentially amenable with other work in the field to develop direct PCR tests for Lyme Disease, as our device could serve as a platform to drastically enhance the concentration of present Borrelia into a small volume.
Download or read book Human Embryonic Stem Cells in Development written by and published by Academic Press. This book was released on 2018-05-22 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: Human Embryonic Stem Cells in Development, Volume 129, the latest release in the Current Topics in Developmental Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as recapitulating pancreas development from human embryonic stem cells in a dish, modeling mammalian gastrulation with embryonic stem cells, and a section on what stem cells tell us about human germ cell biology. Each chapter is written by an international board of authors. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Current Topics in Developmental Biology series
Download or read book Toward Accessible Microfluidics written by Vinay Vishwas Abhyankar and published by . This book was released on 2008 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2007 with total page 960 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Micro Nanofluidics and Lab on Chip Based Emerging Technologies for Biomedical and Translational Research Applications Part B written by and published by Academic Press. This book was released on 2022-01-28 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Micro/Nanofluidics and Lab-on-Chip Based Emerging Technologies for Biomedical and Translational Research Applications - Part B, Volume 187 represents the collation of chapters written by eminent scientists worldwide. Chapters in this new release include Design and fabrication of microfluidics devices for molecular biology applications, Micro/Nanofluidics devices for drug delivery, From organ-on-chip to body-on-chip: the next generation of microfluidics platforms for in vitro drug toxicity testing, Micro/Nanofluidics for high throughput drug screening, Design, fabrication and assembly of lab-on-a-chip and its uses, Advances in microfluidic 3D cell culture for pre-clinical drug development, Tissue and organ culture on lab-on-a chip for biomedical applications, and much more. - Offers a basic understanding of the state-of-the-art design and fabrication of microfluidics/ nanofluidics and lab on chip - Explains how to develop microfluidics/nanofluidic for advanced application such as healthcare, high throughout drug screening, 3D cell culture and organ-on-chip - Discusses the emerging demands and research of micro/nanofluidic based devices in biomedical and translational research applications
Download or read book Integrated Microfluidic Platforms for Investigating Neuronal Networks written by Hyung Joon Kim and published by . This book was released on 2010 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation describes the development and application of integrated microfluidics-based assay platforms to study neuronal activities in the nervous system in-vitro. The assay platforms were fabricated using soft lithography and micro/nano fabrication including microfluidics, surface patterning, and nanomaterial synthesis. The use of integrated microfluidics-based assay platform allows culturing and manipulating many types of neuronal tissues in precisely controlled microenvironment. Furthermore, they provide organized multi-cellular in-vitro model, long-term monitoring with live cell imaging, and compatibility with molecular biology techniques and electrophysiology experiment. In this dissertation, the integrated microfluidics-based assay platforms are developed for investigation of neuronal activities such as local protein synthesis, impairment of axonal transport by chemical/physical variants, growth cone path finding under chemical/physical cues, and synaptic transmission in neuronal circuit. Chapter 1 describes the motivation, objectives, and scope for developing in-vitro platform to study various neuronal activities. Chapter 2 introduces microfluidic culture platform for biochemical assay with large-scale neuronal tissues that are utilized as model system in neuroscience research. Chapter 3 focuses on the investigation of impaired axonal transport by ß-Amyloid and oxidative stress. The platform allows to control neuronal processes and to quantify mitochondrial movement in various regions of axons away from applied drugs. Chapter 4 demonstrates the development of microfluidics-based growth cone turning assay to elucidate the mechanism underlying axon guidance under soluble factors and shear flow. Using this platform, the behaviors of growth cone of mammalian neurons are verified under the gradient of inhibitory molecules and also shear flow in well-controlled manner. In Chapter 5, I combine in-vitro multicellular model with microfabricated MEA (multielectrode array) or nanowire electrode array to study electrophysiology in neuronal network. Also, "diode-like" microgrooves to control the number of neuronal processes is embedded in this platform. Chapter 6 concludes with a possible future direction of this work. Interfacing micro/nano- technology with primary neuron culture would open many doors in fundamental neuroscience research and also biomedical innovation.
Download or read book Biological Applications of Microfluidics written by Frank A. Gomez and published by John Wiley & Sons. This book was released on 2008-02-15 with total page 562 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics has numerous potential applications in biotechnology, pharmaceuticals, the life sciences, defense, public health, and agriculture. This book details recent advances in the biological applications of microfluidics, including cell sorting, DNA sequencing on-a-chip, microchip capillary electrophoresis, and synthesis on a microfluidic format. It covers microfabricated LOC technologies, advanced microfluidic tools, microfluidic culture platforms for stem cell and neuroscience research, and more. This is an all-in-one, hands-on resource for analytical chemists and researchers and an excellent text for students.
Download or read book Advances in Microfluidics written by Xiao-Ying Yu and published by BoD – Books on Demand. This book was released on 2016-11-23 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Increasing innovations and applications make microfluidics a versatile choice for researchers in many disciplines. This book consists of multiple review chapters that aim to cover recent advances and new applications of microfluidics in biology, electronics, energy, and materials sciences. It provides comprehensive views of various aspects of microfluidics ranging from fundamentals of fabrication, flow control, and droplet manipulation to the most recent exploration in emerging areas such as material synthesis, imaging and novel spectroscopy, and marriage with electronics. The chapters have many illustrations showcasing exciting results. This book should be useful for those who are eager to learn more about microfluidics as well as researchers who want to pick up new concepts and developments in this fast-growing field.
