Download or read book Development of DNA Markers Using Random Amplified Polymorphic DNA RAPD for the Identification of Tiger Barbs puntius Tetrazona written by I. Patimah and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development of Randomly Amplified Polymorphic DNA RAPD Markers in Labeo Fimbriatus Blach written by SUDHIR S and published by . This book was released on 2000 with total page 71 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development of PCR based Markers for Identifying Grape Rootstocks written by Hong Xu and published by . This book was released on 1995 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sequence-specific PCR markers were derived from one new and eight previously identified random amplified polymorphic DNA (RAPD) markers for the purpose of identifying grape (Vitis) rootstocks. The markers were developed because the RAPD assay was found to be inconsistent and the original RAPD markers unreliable. Southern hybridization analysis of the RAPD gels with cloned RAPD bands as probes revealed deficiencies of scoring RAPD bands based solely on ethidium bromide staining. In one case, bands of the same size generated by the same primer in different rootstocks -- normally scored as the same marker -- failed to cross-hybridize, implying a lack of homology between the bands. In another case, a band scored as absent based on ethidium bromide staining was detected by hybridization. One of nine RAPD bands was cloned in the present study. All nine were partially sequenced and sequence-specific pairs of primers were synthesized for each for use under stringent PCR conditions. Three of the primer pairs generated products only from the rootstocks from which the original RAPD bands had been cloned; three others produced products from additional rootstocks while still generating polymorphisms; two others generated apparent length variants from some accessions; and one primer pair resulted in a loss of polymorphism. Based on the eight polymorphic markers, five of nine rootstocks could be unambiguously distinguished.
Download or read book A Study of Random Amplified Polymorphic DNA RAPD as Markers in a Linkage Map of Peromyscus Using Two Inbred Strains written by Daniel D. Ririe and published by . This book was released on 1995 with total page 36 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification of DNA Markers in Triticum Aestivum Aegilops Caudata Additions Lines by Randomly Amplified Polymorphic DNA rapd Technology written by Ling Wei and published by . This book was released on 1995 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this study was to identify DNA markers for each of six added C-genome chromosomes in Triticum aestivum L. cv. 'Alceso'-Aegilops caudata L. addition lines using the randomly amplified polymorphic DNA (RAPD) technique. DNA from Ae. caudata, T. aestivum, amphiploid of T. aestivum X Ae. caudata, and six disomic addition lines of wheat having a pair of Ae. caudata chromosomes were used as the template for the amplification of RAPD markers with a total of 58 random 10-mer oligonucleotide primers. Two primers, OPC-08 and OPJ-16, produced one intense band each from the amphiploid of T. aestivum X Ae. caudata and Ae. caudata, which was absent in all six addition lines. Each of these two primers produced a chromosome marker that could be tentatively located to the chromosome CA of Ae. caudata. OPJ-02, OPD-12, OPD-02, OPJ-12, OPD-20, and OPJ-14 produced a marker each for CB, CC, CD, CE, CF, and CG, respectively. OPJ-09 produced C-genome chromosome-specific RAPD markers. Also, OPC-05 and OPJ-19 produced RAPDs from both wheat and Ae. caudata genomes.
Download or read book An Evaluation of Fluorescent Randomly Amplified Polymorphic DNA FRAPD as a Tool for Identifying Species Hybrids and the Application of These Markers to Questions of Hybridization in Two Groups of Ohio River Basin Fishes written by Michael G. Sovic and published by . This book was released on 2011 with total page 157 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Randomly amplified polymorphic DNA (RAPD) has a number of characteristics that make it an attractive option for studies in hybrid zones. However, concerns regarding the repeatability of RAPD data have caused many researchers to opt for other types of genetic markers. In this dissertation, a series of modifications were applied to traditional (RAPD) methodology, and the modified RAPD markers were evaluated for their utility as a tool to identify species hybrids. Levels of repeatability were evaluated for these markers, and the estimated error rates fell within the levels of those generally accepted for studies employing dominant genotyping methods. The repeatability estimates were then incorporated into simulations to directly assess the power of dominant genetic markers in identifying various classes of parental and hybrid individuals. The level of performance varied with the number of loci analyzed, the error rates associated with the loci, and the levels of diversity between the parental taxa. However, many scenarios were identified that are likely to be encountered in natural populations in which the simulated markers performed very well, suggesting that the modified RAPD markers may serve as a viable option in many hybridization studies. Finally, two case studies are presented in which the modified RAPD markers are applied to address questions of hybridization in freshwater fishes of the Ohio River basin. In the first case study, hybrid saugeye (Sander vitreus x Sander canadensis), which are artificially produced and stocked into waters throughout Ohio, were evaluated to determine whether reproduction occurs by these fish. No evidence of reproduction was observed in the inland reservoirs of Ohio, suggesting that reproduction among F1 hybrid saugeye occurs very rarely, or not at all. However, evidence of reproduction by saugeye is presented from Sander spp. individuals collected from the Ohio River, where native populations of both parental species occur. In the second case study, fishes of the genus Carpiodes are examined in order to determine whether hybridization occurs in this group. Evidence of hybridization is presented for these fishes, and a combination of molecular data and morphological data suggest that the rates of hybridization may vary among different sympatric communities of Carpiodes spp. in the Ohio River basin. The direction of interspecific gene flow resulting from the hybridization also appears to be biased, with C. cyprinus sending genes into C. carpio more often than the reverse. The results of each of these two case studies are discussed in the context of their potential influences on the evolutionary trajectory of these groups. Finally, as part of the analysis of hybridization in Carpiodes, C. cyprinus was identified as a nonmonophyletic taxon according to mitochondrial cytochrome b sequence data. The patterns of genetic diversity observed at this gene suggest the potential that cryptic diversity occurs within C. cyprinus. Specifically, specimens collected from the Susquehanna River basin may be distinct from those in the Great Lakes and Ohio River basins. These data suggest that a reevaluation of the taxonomy of this group is warranted.
Download or read book DNA Markers written by Gustavo Caetano-Anollés and published by Wiley-Liss. This book was released on 1997-09-16 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the four decades since the discovery of DNA, molecular genetics and biotechnology have undergone a revolution in the research and application of genetic testing techniques. As a result, these fields have witnessed an explosion of both information and controversy, and the focus of public awareness of the significance of molecular biotechnology in everyday life has leaped from journal abstracts to front-page news. Molecular biotechnology provides important tools for the identification of specific nucleic acid sequences, some of them linked to important traits such as those conferring resistance to disease. Such DNA markers are used commonly in genetic research and in other applications, such as DNA fingerprinting. Researchers can use DNA markers to follow individual traits in different environments and hosts, increasing our understanding of the constitution, diversity, and evolution of genetic material. DNA Markers: Protocols, Applications, and Overviews offers valuable new information on this rapidly growing field. This compendium of research highlights the most recently discovered DNA markers and their application to the analyses of genomes of low and high complexity, with a focus on recently developed DNA marker systems as tools for DNA fingerprinting, genome analysis, molecular systematics, taxonomy, and phylogenetic analysis. The Protocols section of the book contains detailed, step-by-step instructions on how to perform selected DNA marker experiments, providing a useful laboratory tool. Subjects covered by top specialists include: Designed as a bench-top reference for the laboratory as well as a complete guide to the latest research, DNA Markers: Protocols, Applications, and Overviews is a vital resource for both novices and experienced practitioners involved in research in molecular genetics or biotechnology. A global overview of DNA markers * Molecular hybridization * RAPD, AP-PCR, DAF and AFLP analysis * Microbial Genomic fingerprinting * Simple sequence repeat DNA markers * Genetic BIT analysis * Differential display of RNA * The status of DNA fingerprinting: population databases * Arbitrarily amplified DNA in ecology and evolution
Download or read book Inheritance of Random Amplified Polymorphic DNA RAPD Molecular Marker in Brown Planthopper Nilaparvata Lugens written by A. Latif and published by . This book was released on 1999 with total page 5 pages. Available in PDF, EPUB and Kindle. Book excerpt: RAPD molecular markers were tested for segregation in two families of the brown planthopper, N. lugens. Single pair matings between individuals from two sympatric populations of N lugens, one from rice and the other from Leersia hexandra, a weed grass were used to produce the families. Four short RAPD primers amplified 41 bands (markers) in these two families. Twenty one of the markers could not be tested because no segregation was observed while the other 20 markers showed segregation in the progenies of the two families and were tested for conformity to simple Mendelian ratios. Twenty segregating markers showed ratios in conformity with them being dominant Mendelian genetic traits.
Download or read book Identification and Characterization of Chromosome specific Randomly Amplified Polymorphic DNA RAPD Markers in Triticum Aestivum Aegilops Caudat Addition Lines written by Lihua Sun and published by . This book was released on 1998 with total page 166 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Use of RAPD Random Amplified Polymorphic DNA Markers to Increase the Cross compatibility Between Fagopyrum Esculentum and F Tataricum and to Analyze the Resulting Embryos for Their Hybridity written by Dimuth S. K. Siritunga and published by . This book was released on 1994 with total page 62 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development of the Random Amplified Polymorphic DNA RAPD Technique to Measure the Effects of Genotoxins in Aquatic Organisms written by Franck Andre Atienzar and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Variation of Random Amplified Polymorphic DNA RAPD Markers in Pecan Carya Illinoensis written by Michelle Imbalzano and published by . This book was released on 1993 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Segregation of Random Amplified Polymorphic DNA RAPD and Microsatellite Markers in Backcross Progeny of Peromyscus written by David A. McClellan and published by . This book was released on 1994 with total page 72 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Investigation of Random Amplified Polymorphic DNA RAPD as Markers for Soluble Solids in Onion written by Ruwanthi Wettasinghe and published by . This book was released on 1996 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Identification of Species Specific DNA Marker as Barcode Sequence from Greater Indian One Horned Rhinoceros Rhinoceros unicornis from North East India written by Tarikul Mazumder and published by GRIN Verlag. This book was released on 2014-08-05 with total page 51 pages. Available in PDF, EPUB and Kindle. Book excerpt: Master's Thesis from the year 2014 in the subject Biology - Geobiology, Assam University, language: English, abstract: The North East India is a reservoir of rich biodiversity for much of India’s flora and fauna, and as a consequence, the region is one of the richest in biological values. DNA based species identification or DNA barcoding is an exciting tool for documenting biodiversity with a gene sequence. Here we focused on the development of DNA barcode sequence of Greater Indian one-horned Rhinoceros [Rhinoceros unicornis] by determining the DNA sequence of its mitochondrial 5 ́ COI gene region using cross primer design for PCR amplification. The cross species primer pairs of forward primer fish [FISH COX F] and reverse primer invertebrate [INV R] DNA barcoding, successfully amplified the barcode sequence of R. unicornis which is confirmed through similarity search in National Centre for Biotechnology [NCBI, www.ncbi.nlm.nih.gov] and Barcode of life [BOLD] database and submitted to NCBI-GenBank with accession no. JN417004 is the first COI barcode sequence of R. unicornis of Greater Indian one-horned Rhino. We observed the maximum identification of the query sequence in case of R. unicornis (99%) and R. sondaicus (93%). Mean pair wise distance was computed using K2P to check the inter species divergence between all species in each genus among the members of Rhinoceretidae family in COI region taking 632 bp into consideration, within species was found to be 0.002 as the maximum and 0.000 as the minimum and that of in between species was found to be 0.064 as the maximum and 0.000 as the minimum. The study represents development of COI species specific DNA barcode of R. unicornis with regard to conservation and trade aspect. The cross species primer pair to amplify barcode gene for a species for which no specific primer are available represents a noble approach for barcoding on those areas like North East India where biological diversity is high.
Download or read book Genome Mapping and Genomics in Fishes and Aquatic Animals written by Thomas D. Kocher and published by Springer Science & Business Media. This book was released on 2008-06-07 with total page 195 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mapping of animal genomes has generated huge databases and several new concepts and strategies, which are useful to elucidate origin, evolution and phylogeny. Genetic and physical maps of genomes further provide precise details on chromosomal location, function, expression and regulation of academically and economically important genes. The series Genome Mapping and Genomics in Animals provides comprehensive and up-to-date reviews on genomic research on a large variety of selected animal systems, contributed by leading scientists from around the world. This volume summarizes the first era of genomic studies of aquaculture species, in which the tools and resources necessary to support whole-genome sequencing were developed. These tools will enhance efforts toward selective breeding of aquaculture species. Included in this volume are summaries of work on salmonids, cyprinids, catfish, tilapias, European sea bass, Japanese flounder, shrimps and oysters.
Download or read book Random Amplified Polymorphic DNA RAPD Assay Pf Pasteurella Haemolytica written by Trevor Van Woerden and published by . This book was released on 1994 with total page 36 pages. Available in PDF, EPUB and Kindle. Book excerpt:
