Download or read book Development of a Dual Joystick controlled Optical Trapping and Cutting System for Optical Micro manipulation of Cells written by Marcellinus Stevie Harsono and published by . This book was released on 2011 with total page 145 pages. Available in PDF, EPUB and Kindle. Book excerpt: In many situations there is a need to physically manipulate microscopic objects with as much dexterity as our own hands provide in the macroscopic world. An example would be applying stresses onto cells in order to determine their mechanical properties. Our existing microscope-laser experimental system is capable of manipulating microscopic objects using two optical tweezers and one optical scissors to move and cut these objects, respectively. Despite these capabilities, however, the point-and-click user interface for optical trapping and cutting was cumbersome and hard to use, limiting the system's potential for micro-manipulation. In order to resolve this limitation, a new, more intuitive and hands-on user interface using two joysticks was designed and developed from the ground up in order to provide responsive, real-time control of the optical tweezers and scissors for microscopic manipulation. This new joystick user interface was then used to verify whether or not forces other than those due to microtubule dynamics act upon chromosomes during mitosis, tested in mitotic PtK2 and Indian Muntjac cells by 1) depolymerizing microtubules using nocodazole, and 2) disrupting microtubules using laser ablation with optical scissors, and subsequently attempting to freely manipulate chromosomes using joystick-controlled optical trapping.
Download or read book Optical Trapping Laser Tweezers and Nanosurgery Laser Scissors written by Michael W. Berns and published by Frontiers Media SA. This book was released on 2022-02-03 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Light Robotics Structure mediated Nanobiophotonics written by Jesper Glückstad and published by Elsevier. This book was released on 2017-05-19 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: Light Robotics – Structure-Mediated Nanobiophotonics covers the latest means of sculpting of both light and matter for achieving bioprobing and manipulation at the smallest scales. The synergy between photonics, nanotechnology and biotechnology spans the rapidly growing field of nanobiophotonics. Nanoscale resolutions enable optical scientists to assess ever more accurate information. However, scientific hypothesis testing demands tools, not only for observing nanoscopic phenomena, but also for reaching into and manipulating nanoscale constituents. Taking an application based focus, this book explores how nanophotonics can productively be used in both the biomedical and life sciences, allowing readers to clearly see how structure-mediated nanobiophotonics can be used to increase our engineering toolbox for biology at the smallest scales. This book will be of great use to researchers and scientists working in the fields of optics and photonics. It will also be of great value to those working in the field of biotechnology, showcasing how nanotechnology can help provide new, effective ways to solve biomedical problems. - Presents cutting-edge research on the principles, mechanisms, optical techniques, fabrication, modeling, devices and applications of nanobiophotonics - Brings together the diverse field of structure-mediated nanobiophotonics into one coherent volume - Showcases how nanophotonics can be used to create new, more effective micro- and nano-biodevices
Download or read book Optical Trapping and Manipulation written by Philip Jones and published by MDPI. This book was released on 2020-11-25 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: We are pleased to present “Optical Trapping and Manipulation: From Fundamentals to Applications”, a Special Issue of Micromachines dedicated to the latest research in optical trapping. In recognition of the broad impact of optical manipulation techniques across disciplines, this Special Issue collected contributions related to all aspects of optical trapping and manipulation. Both theoretical and experimental studies were welcome, and applications of optical manipulation methods in fields including (but not limited to) single molecule biophysics, cell biology, nanotechnology, atmospheric chemistry, and fundamental optics were particularly welcome in order to showcase the breadth of the current research. The Special Issue accepted diverse forms of contributions, including research papers, short communications, methods, and review articles representing the state-of-the-art in optical trapping.
Download or read book Optical Trapping Cell Manipulation and Robotics written by and published by . This book was released on 1989 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: A new type of analytical and preparative cytometric instrument was developed. The instrument combines image analysis and machine vision with single cell and chromosome manipulation by means of optical trapping. A proof-of-principle instrument, OCAM, has the ability to locate and analyze biological particles inside an enclosed manipulation chamber, as well as the ability to move and position particles according to preprogrammed protocols. Preliminary results and potential biological applications of such a microrobot are discussed. 12 refs., 8 figs.
Download or read book VCSEL Based Optical Trapping Systems for Microfluidic Applications written by Andrea Kroner and published by Cuvillier Verlag. This book was released on 2011-01-17 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical trapping and manipulation by laser beams offers the unique possibility to handle single micrometer-sized particles such as living cells without any mechanical contact, damage or contamination. A second hot topic in biology is microfluidics, where the examination of biological samples in channel structures with widths below 100 µm reduces the used sample volume significantly. While the combination of both techniques results in attractive lab-on-a-chip structures for particle sorting and analysis, the commonly bulky trapping setup is contradictory to the miniaturized concept. Here, the use of vertical-cavity surface-emitting lasers (VCSELs) as light sources in optical trapping systems allows a strong reduction of the setup complexity owing to the small dimensions, low cost and high beam quality of these devices. This thesis gives a detailed study on optical manipulation systems based on vertically emitting laser diodes. A standard optical tweezers setup as well as a novel, miniaturized system, the so-called integrated optical trap are investigated. The latter aims for particle separation and sorting in microfluidics resulting in low-cost, portable modules. A classical optical tweezers system based on a high numerical aperture objective in combination with a VCSEL light source is investigated. Standard multi-mode as well as single-mode surface relief VCSELs are used as laser source. With both kinds of VCSELs, optical trapping of polystyrene particles of sizes ranging from 4 to 15µm is demonstrated with some milliwatts of optical power at the sample stage. A maximum trapping force of 4.4 pN for 15 µm particles is achieved with the multi-mode laser, proving the suitability of multi-mode lasers for optical manipulation despite their inferior beam profile. By using two-dimensional VCSEL arrays instead of solitary lasers, the system is extended to a multiple optical tweezers setup in a straightforward manner. To avoid any additional optics, densely packed VCSEL arrangements with a device spacing of less than 25 µm are used, where a novel fabrication process allows the seamless integration of the inverted surface relief technique for enhanced beam quality. By electrical switching between individual devices of the array, non-mechanical particle translation with velocities of up to 12 µm/s is achieved. With a tilted linear VCSEL array, an optical lattice is generated in the optical tweezers setup, and continuous deflection of particles is realized. By substituting the sample stage in the optical tweezers setup with a microfluidic chip fabricated from polydimethylsiloxane (PDMS), particle redirection at a channel junction is realized using a solitary VCSEL source as well as a tilted linear VCSEL array. For the latter, the particles are deflected when passing the optical lattice, thus, the position of the lasers is fixed and no moving parts are necessary, which further reduces the setup complexity. To achieve a drastic miniaturization of the trapping setup, namely the integrated optical trap, the laser source is placed directly underneath the sample chamber. A weakly focused laser beam is generated in the particle solution by integrating an additional microlens on the VCSEL output facet. To determine appropriate lens geometries, the beam propagation inside the integrated trap structure is calculated and the thermal reflow process for lens fabrication is studied in detail concerning lens diameter, reflow temperature and substrate material. By combining the microlens with the inverted relief technique, the quality of the focused beam is strongly improved with respect to divergence, transverse beam profile and beam diameter, where a minimum of 7 µm is measured at the focal point. With first solitary integrated optical traps, deflection, levitation and transverse trapping of 10 µm polystyrene particles is demonstrated for optical powers of 5mW. In a next step, integrated optical trap arrays are realized based on closely spaced twodimensional arrangements of lensed relief VCSELs. To transfer the continuous deflection scheme demonstrated in the classical tweezers setup to the integrated trap, linear arrays of parallel working VCSELs are investigated. To support the design of the multiple integrated trap structure, a simulation of the optical deflection process is performed. Here, a dependence on the geometric and material properties of the particles is predicted, so applications in microfluidic particle sorting are intended. Compact and portable modules are obtained by integrating the laser chip with the microfluidic chip using flip-chip bonding. Although the finished modules show strong heating of the VCSEL chip resulting in a significant reduction of the device performance, simultaneous trapping as well as continuous particle deflection was successfully demonstrated with a total optical power of just 5mW. The results presented in this work demonstrate the potential of VCSELs as laser sources for optical trapping and microparticle manipulation. In conventional optical tweezers setups, the use of VCSELs reduces the setup complexity significantly, while first prototypes of ultra-compact integrated optical traps based on VCSELs confirm the feasibility of portable and inexpensive microfluidic sorting systems.
Download or read book Optical Trapping and Optical Micromanipulation IX written by Kishan Dholakia and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Annotation Includes Proceedings Vol. 7821.
Download or read book Optical Trapping and Manipulation of Neutral Particles Using Lasers written by Arthur Ashkin and published by World Scientific Publishing Company Incorporated. This book was released on 2006 with total page 915 pages. Available in PDF, EPUB and Kindle. Book excerpt: This important volume contains selected papers and extensive commentaries on laser trapping and manipulation of neutral particles using radiation pressure forces. Such techniques apply to a variety of small particles, such as atoms, molecules, macroscopic dielectric particles, living cells, and organelles within cells. These optical methods have had a revolutionary impact on the fields of atomic and molecular physics, biophysics, and many aspects of nanotechnology.In atomic physics, the trapping and cooling of atoms down to nanokelvins and even picokelvin temperatures are possible. These are the lowest temperatures in the universe. This made possible the first demonstration of Bose-Einstein condensation of atomic and molecular vapors. Some of the applications are high precision atomic clocks, gyroscopes, the measurement of gravity, cryptology, atomic computers, cavity quantum electrodynamics and coherent atom lasers.A major application in biophysics is the study of the mechanical properties of the many types of motor molecules, mechanoenzymes, and other macromolecules responsible for the motion of organelles within cells and the locomotion of entire cells. Unique in vitro and in vivo assays study the driving forces, stepping motion, kinetics, and efficiency of these motors as they move along the cell's cytoskeleton. Positional and temporal resolutions have been achieved, making possible the study of RNA and DNA polymerases, as they undergo their various copying, backtracking, and error correcting functions on a single base pair basis.Many applications in nanotechnology involve particle and cell sorting, particle rotation, microfabrication of simple machines, microfluidics, and other micrometer devices. The number of applications continues to grow at a rapid rate.The author is the discoverer of optical trapping and optical tweezers. With his colleagues, he first demonstrated optical levitation, the trapping of atoms, and tweezer trapping and manipulation of living cells and biological particles.This is the only review volume covering the many fields of optical trapping and manipulation. The intention is to provide a selective guide to the literature and to teach how optical traps really work.
Download or read book Optical Trapping and Optical Micromanipulation VII written by and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Optical Manipulation of Biological Cell Without Measurement of Cell Velocity written by and published by . This book was released on with total page 4 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical tweezer is a useful tool for non-contact micromanipulation tasks because it can manipulate biological cells precisely without causing damage to the cells. In many optical manipulation techniques, the measurement of the velocity of the cell is necessary. Since it is difficult to measure the velocity of the cell, the velocity information is usually obtained by differentiating the position of the cell in image space, which may result in degraded performance of the control system due to estimation error and the noises induced in differentiation. In this paper, an adaptive observer technique is proposed for optical manipulation of cell with a low Reynolds' number, without measurement of the velocity of the cell. By using the proposed observer technique, a desired position input of the laser beam without measurement of the velocity of the cell is also developed. The stability of closed-loop system is analyzed by using Lyapunov-like method. Experimental results are presented to illustrate the performance of the proposed control method.
Download or read book Optical Trapping and Optical Micromanipulation XVI written by Kishan Dholakia and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book A Time shared Dual Optical Trap with Sub nanometer Image based Position Resolution for Hybrid Instruments written by Jessica Killian and published by . This book was released on 2017 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: Optical trapping is a powerful and sensitive technique that enables the direct measurement and manipulation of biological samples on the piconewton and nanometer scales. In examining the evolution and maturation of optical trapping instrumentation in the field of single-molecule biophysics, two main thrusts become apparent; a subset of instruments have aimed to push the limits of resolution, seeking to capture the finest details of biomechanical processes, while a second subset of instruments have explored the integration of optical trapping with other measurement techniques, with the goal of creating multidimensional, hybrid instruments. Unfortunately, many hybrid trapping instruments are forced to sacrifice measurement resolution, or relinquish their measurement capabilities entirely, when the combination of techniques cannot accommodate the stringent demands of traditional optical trapping detection methods. Thus, the full potential of hybrid optical trapping instruments has remained elusive. We have taken on this challenge by developing a highly-versatile optical tweezers instrument that can achieve sub-nanometer position resolution without the use of a second microscope objective, making high-resolution optical trapping measurements uniquely compatible with a broad array of bulky and/or opaque sample-side devices. Our instrument consists of a time-shared, dual optical trap implemented in an entirely custom-built, free-standing fluorescence microscope, with camera-based position detection. We have developed a robust and accurate set of tracking techniques that allow us to make the first direct measurements of relative bead displacement within an optical trap with sub-nanometer resolution using image tracking. These capabilities pave the way for the next generation of optical tweezer hybrids, which we envision as versatile tools that can integrate multiple measurement modalities and complex substrates without sacrificing resolution, to expand the breadth and clarity of our window into the microscopic and sub-microscopic biological worlds. ...
Download or read book Enhanced Optical Tweezing written by Unė Gabrielė Būtaitė and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Optical Tweezers written by Philip H. Jones and published by Cambridge University Press. This book was released on 2015-12-03 with total page 565 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive guide to the theory, practice and applications of optical tweezers, combining state-of-the-art research with a strong pedagogic approach.
Download or read book Manipulating Microscopic Objects A Study on Optical Tweezers and Acoustic Levitation written by Marius Martin and published by GRIN Verlag. This book was released on 2023-03-30 with total page 37 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bachelor Thesis from the year 2023 in the subject Physics - Applied physics, University of Aberdeen, language: English, abstract: This thesis investigates two powerful techniques for manipulating and studying microscopic particles: optical tweezers and acoustic levitation. Optical tweezers use the force of light to trap and manipulate small particles, while acoustic levitation uses sound waves to levitate particles in mid-air. The first part of the thesis focuses on the construction and optimization of an optical tweezers system. The system is build using high-powered laser, optics for focusing the laser beam, and a position detection and control system. the second part of this thesis explores the use of acoustic levitation for the manipulation of micro spheres. The acoustic levitation system is designed and constructed using 72 transducers. The system is characterized using measurements of the acoustic field and the resulting levitation forces. The levitation of micro spheres is demonstrated. This thesis would provide a detailed investigation of two powerful techniques for the manipulation of microscopic particles.
Download or read book Optical Trapping and Manipulation written by Philip Jones and published by . This book was released on 2020 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: We are pleased to present “Optical Trapping and Manipulation: From Fundamentals to Applications”, a Special Issue of Micromachines dedicated to the latest research in optical trapping. In recognition of the broad impact of optical manipulation techniques across disciplines, this Special Issue collected contributions related to all aspects of optical trapping and manipulation. Both theoretical and experimental studies were welcome, and applications of optical manipulation methods in fields including (but not limited to) single molecule biophysics, cell biology, nanotechnology, atmospheric chemistry, and fundamental optics were particularly welcome in order to showcase the breadth of the current research. The Special Issue accepted diverse forms of contributions, including research papers, short communications, methods, and review articles representing the state-of-the-art in optical trapping.
Download or read book Lab on Fiber Technology written by Andrea Cusano and published by Springer. This book was released on 2014-07-29 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on a research field that is rapidly emerging as one of the most promising ones for the global optics and photonics community: the “lab-on-fiber” technology. Inspired by the well-established "lab on-a-chip" concept, this new technology essentially envisages novel and highly functionalized devices completely integrated into a single optical fiber for both communication and sensing applications. Based on the R&D experience of some of the world's leading authorities in the fields of optics, photonics, nanotechnology, and material science, this book provides a broad and accurate description of the main developments and achievements in the lab-on-fiber technology roadmap, also highlighting the new perspectives and challenges to be faced. This book is essential for scientists interested in the cutting-edge fiber optic technology, but also for graduate students.
