Download or read book Application of Next Generation Sequencing NGS and CRISPR Cas Systems in The Diagnosis of Infectious Diseases written by Yanfei Chen and published by Frontiers Media SA. This book was released on 2023-03-03 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book CRISPR Cas System Based Diagnosis of Infectious Diseases written by Roohi Bansal and published by . This book was released on 2021-03-08 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since its discovery as a part of the bacterial defense mechanism, the Nobel Prize-winning technology CRISPR-Cas system has revolutionized the fields of genome editing and genetic engineering. Beyond gene-editing ability, scientists have leveraged its potential in the diagnosis of infectious diseases including COVID-19. This book provides a detailed understanding of CRISPR-based rapid and point-of-care diagnostic kits like SHERLOCK, DETECTR, FELUDA, AIOD CRISPR-Cas12a, etc. for the diagnosis of infectious diseases including COVID-19; the importance of isothermal nucleic acid amplification processes like RPA and LAMP; and how the results of the CRISPR-based tests can be interpreted using paper-based lateral flow strips. Because of the ease of performing the test and simple interpretation of the test results, CRISPR-based tests can be used at airports, ports, clinics, schools, etc., for better disease diagnosis, monitoring, management, and containment of infectious diseases like COVID-19. Additionally, this book discusses Monoclonal Antibodies, which have revolutionized the treatment for cancer, arthritis, autoimmune diseases, etc. This book also talks about various strategies to isolate monoclonal antibodies from the COVID-19 recovered people and different ways to engineer these antibodies using hybridoma technology.

Download or read book Next Generation Sequencing NGS for Rare Diseases Diagnosis written by Xiu-An Yang and published by Frontiers Media SA. This book was released on 2022-01-28 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Improving the Clinical Effectiveness of Metagenomic Next Generation Sequencing mNGS in Infection Disease Diagnosis and Treatment Linking the NGS Specialists and Clinicians volume II written by Beiwen Zheng and published by Frontiers Media SA. This book was released on 2023-12-28 with total page 133 pages. Available in PDF, EPUB and Kindle. Book excerpt: Timely and accurate pathogen diagnosis is critical for effective treatment, outbreak prevention and precise antibiotics administration of infectious diseases, which remains a challenge in clinical practice. Metagenomic next generation sequencing (mNGS) allows researchers and laboratory specialists to analyze the mixed collection of sequencing reads in human clinical samples, including sequences from bacteria, viruses, fungi and parasites besides the host. This new technology shows great potential in pathogen diagnosis. The sensitivity and faster turnaround time are higher than conventional clinical microbiology tests, especially for fastidious and atypical pathogens. However, current mNGS based pathogen detection and diagnosis are facing challenges from both technical and practical aspects. First, sequencing noise can be introduced from different steps such as samples preparation, sequencing, data analysis and reporting algorithm. Also, the interpretation of the results such as detection limits and detection rates are not straightforward to clinicians, compared with traditional culture-based technologies. Such challenges and considerations should be fully addressed before the wild application of mNGS as a pathogen detection tool. Further, these clarifications might help to properly find the best clinical application scenarios, refine the medical treatments, and improve the clinical effectiveness of mNGS in complex infectious diseases. This Research Topic is focused on the application of mNGS for infectious diseases diagnosis and treatment, including experiment process and clinical usage. Its purpose is to level up the clarification of NGS process, to support the development of efficient experiment methods, data interpretation algorithms and to report good examples of related clinical applications. We hope this Research Topic could facilitate the discovery of novel methods and help NGS specialists and clinicians to know mutual concerns and work together to improve the clinical effectiveness of mNGS.

Download or read book Next Generation Sequencing and CRISPR Cas Editing in Plant Virology written by Ahmed Hadidi and published by Frontiers Media SA. This book was released on 2021-11-26 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Clinical Applications for Next Generation Sequencing written by Urszula Demkow and published by Academic Press. This book was released on 2015-09-10 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Clinical Applications for Next Generation Sequencing provides readers with an outstanding postgraduate resource to learn about the translational use of NGS in clinical environments. Rooted in both medical genetics and clinical medicine, the book fills the gap between state-of-the-art technology and evidence-based practice, providing an educational opportunity for users to advance patient care by transferring NGS to the needs of real-world patients. The book builds an interface between genetic laboratory staff and clinical health workers to not only improve communication, but also strengthen cooperation. Users will find valuable tactics they can use to build a systematic framework for understanding the role of NGS testing in both common and rare diseases and conditions, from prenatal care, like chromosomal abnormalities, up to advanced age problems like dementia. Fills the gap between state-of-the-art technology and evidence-based practice Provides an educational opportunity which advances patient care through the transfer of NGS to real-world patient assessment Promotes a practical tool that clinicians can apply directly to patient care Includes a systematic framework for understanding the role of NGS testing in many common and rare diseases Presents evidence regarding the important role of NGS in current diagnostic strategies

Download or read book Application of Next Generation Sequencing NGS in Infection Prevention written by Michael Kemp and published by Frontiers Media SA. This book was released on 2022-07-04 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Next Generation Sequencing written by Jerzy Kulski and published by BoD – Books on Demand. This book was released on 2016-01-14 with total page 466 pages. Available in PDF, EPUB and Kindle. Book excerpt: Next generation sequencing (NGS) has surpassed the traditional Sanger sequencing method to become the main choice for large-scale, genome-wide sequencing studies with ultra-high-throughput production and a huge reduction in costs. The NGS technologies have had enormous impact on the studies of structural and functional genomics in all the life sciences. In this book, Next Generation Sequencing Advances, Applications and Challenges, the sixteen chapters written by experts cover various aspects of NGS including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS applications in basic and applied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.

Download or read book Improving the Clinical Effectiveness of Metagenomic Next Generation Sequencing mNGS in Infection Disease Diagnosis and Treatment Linking the NGS Specialists and Clinicians written by and published by Frontiers Media SA. This book was released on 2023-10-17 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Next Generation Sequencing Technologies in Medical Genetics written by C. Alexander Valencia and published by Springer Science & Business Media. This book was released on 2013-10-16 with total page 101 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book introduces readers to Next Generation Sequencing applications in medical genetics. The authors discuss the direct application of next-generation sequencing to medicine, specifically, laboratory medicine or molecular diagnostics. The first part of the book contains chapters on sanger sequencing, NGS technologies, targeted-amplification and capture, and exome sequencing. The second part of the book focuses on genetic disorders diagnoses by NGS, prenatal diagnosis, muscular dystrophies, mitochondrial disorders diagnosis, and challenges in molecular diagnosis. Recent developments and potential future trends in NGS sequencing applications are highlighted, as well.​

Download or read book Next generation sequencing NGS and annotation of three Staphyloccocus aureus strains written by Theodore Ngwana and published by GRIN Verlag. This book was released on 2018-02-05 with total page 29 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientific Essay from the year 2018 in the subject Medicine - Diagnostics, University of Salford (College of Science and Technology), course: Msc Biomedical Science, language: English, abstract: Next Generation Sequencing, one of the applications of genomics, employs bioinformatics tools and other molecular biology techniques such as the Polymerase Chain Reaction (PCR) and electrophoresis to diagnose diseases. Once sequenced, our assembled genome sequenced can be annotated to identify target genes and compared to a reference genome for similarities such as the case of BLAST, which is used to establish evolutionary relationships between species. The sequences can also be compared for variants. In this report Illumina-based next generation sequencing was used to sequence the genomes of three Staphylococcus aureus strains which are the methicillin resistant strain (MRSA), methicillin sensitive strain (MSSA) and an unknown strain of S. aureus (U). This involved an array of techniques, from the extraction of genomic DNA by way of the QIAGEN DNeasy DNA extraction kit, to quality control of our purified DNA by 1% agarose gel electrophoresis, Nanodrop and Qubit. It then moved on to preparation of DNA libraries from our genomic DNA extract, with the QIAGEN® QIAseq FX DNA Library Kit, from where the libraries went through an Illumina MiSeq sequencer. Raw sequence data was then assembled and analysed with genome annotation tools such as Atermis, Artemis Comparison Tool, WGSA and Abricate. Annotation of our S. aureus species revealed antimicrobial resistance genes. Abricate produced 10 antimicrobial genes for MRSA, 5 for MSSA and 6 for U. Meanwhile Artemis annotation gave 16 antimicrobial resistance genes for MRSA, 13 for MSSA and 14 for U. Information on antibiotic susceptibility of the three bacterial species can then be used to determine the best possible treatment for individuals with S. aureus infections, thus proving that next generation sequencing and genome annotation can serve as an alternative for routine disc antimicrobial susceptibility techniques to diagnose and treat infectious diseases.

Download or read book Reprogramming the Genome Applications of CRISPR Cas in non mammalian systems part A written by and published by Academic Press. This book was released on 2021-03-28 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reprogramming the Genome: Applications of CRISPR-Cas in Non-mammalian Systems, Part A presents a collation of chapters written by global, eminent scientists. CRISPR-Cas9 system is an RNA-mediated immune system of bacteria and archaea that protects from bacteriophage infections. It is one of the revolutionized technologies to uplift biology to the next stages. Chapters in this release include An Introduction and applications of CRISPR-Cas Systems, History, evolution and classification of CRISPR-Cas associated systems, CRISPR based bacterial genome editing and removal of pathogens, CRISPR based genome editing and removal of human viruses, CRISPR based development of RNA editing and diagnostic platform, and much more. Additional sections cover Genome engineering in insects for control of vector borne diseases, Development of insect cell line using CRISPR technology, CRISPRing protozoan parasites to better understand the biology of diseases, CRISPR based genome editing of Caenorhabditis elegans, and a variety of other important topics. Offers a basic understanding and clear picture of genome editing CRISPR-Cas systems in different organisms Explains how to create an animal model for disease diagnosis/research and reprogram CRISPR for removal of virus, bacteria, fungi, protozoan, and many more Discusses the advances, patents, applications, challenges and opportunities in CRISPR-Cas9 systems in basic sciences, biomedicine, virology, bacteriology, molecular biology, and many more

Download or read book Next Generation Sequencing written by Lee-Jun C. Wong and published by Springer Science & Business Media. This book was released on 2013-05-31 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, owing to the fast development of a variety of sequencing technologies in the post human genome project era, sequencing analysis of a group of target genes, entire protein coding regions of the human genome, and the whole human genome has become a reality. Next Generation Sequencing (NGS) or Massively Parallel Sequencing (MPS) technologies offers a way to screen for mutations in many different genes in a cost and time efficient manner by deep coverage of the target sequences. This novel technology has now been applied to clinical diagnosis of Mendelian disorders of well characterized or undefined diseases, discovery of new disease genes, noninvasive prenatal diagnosis using maternal blood, and population based carrier testing of severe autosomal recessive disorders. This book covers topics of these applications, including potential limitations and expanded application in the future. ​

Download or read book Landscape of Next Generation Sequencing Using Pattern Recognition written by Saurav Mallik and published by CRC Press. This book was released on 2024-10-23 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on an eminent technology called next generation sequencing (NGS) which has entirely changed the procedure of examining organisms and will have a great impact on biomedical research and disease diagnosis. Numerous computational challenges have been brought on by the rapid advancement of large-scale next-generation sequencing (NGS) technologies and their application. The term ""biomedical imaging"" refers to the use of a variety of imaging techniques (such as X-rays, CT scans, MRIs, ultrasounds, etc.) to get images of the interior organs of a human being for potential diagnostic, treatment planning, follow-up, and surgical purposes. In these circumstances, deep learning, a new learning method that uses multi-layered artificial neural networks (ANNs) for unsupervised, supervised, and semi-supervised learning, has attracted a lot of interest for applications to NGS and imaging, even when both of these data are used for the same group of patients. The three main research phenomena in biomedical research are disease classification, feature dimension reduction, and heterogeneity. AI approaches are used by clinical researchers to efficiently analyse extremely complicated biomedical datasets (e.g., multi-omic datasets. With the use of NGS data and biomedical imaging of various human organs, researchers may predict diseases using a variety of deep learning models. Unparalleled prospects to improve the work of radiologists, clinicians, and biomedical researchers, speed up disease detection and diagnosis, reduce treatment costs, and improve public health are presented by using deep learning models in disease prediction using NGS and biomedical imaging. This book influences a variety of critical disease data and medical images.

Download or read book Mapping and Prioritizing Criteria and Alternative Operational Models for an Optimal Adoption of Next Generation DNA Sequencing Technology for Diagnosing Infectious Diseases in Hospital Settings written by Vered Mintzer and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "Objectives: 1. To map and determine the relative importance of clinical, operational and economic criteria for optimal integration of NGS technology, specifically its application for whole genome sequencing (WGS) in infectious disease diagnostic processes in hospitals (henceforth: the decision). 2. To examine whether these clinical, operational and economic criteria for the decision, are prioritized differently across different operational models. 3. To examine whether these criteria are prioritized differently among clinical microbiology specialists with vast experience with WGS compared to those with little or no experience." -- Abstract.

Download or read book The CRISPR Cas System written by Muhammad Jamal and published by Caister Academic Press Limited. This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of CRISPR/Cas technology for genome editing suggests many potential applications, including the alteration of the germline of humans, animals and food crops. The speed and efficiency of the CRISPR/Cas system make it a potentially useful system for gene therapy. In this volume expert international authors provide a useful and timely review of the applications of the CRISPR/Cas system across diverse fields and explore further avenues and research directions of this novel and powerful editing technology. The technology and its application are reviewed with respect to reproduction and development, immunity and genetic diseases, system structure and system specificity. Some of the potential problems of the CRISPR/Cas system are also discussed, in particular the specificity of the system: this remains an important topic as improvement could lead to the more direct and efficient use of the CRISPR/Cas system in clinical settings. The authors also debate ethical concerns associated with this powerful new technology. This volume is a rigorous review of the applications and new opportunities for the CRISPR/Cas system and provides a stimulus for current and future research. An invaluable guide for all scientists working in the fields of genome editing and gene therapy the book is also recommended for all life sciences libraries.

Download or read book Emerging Human Viral Diseases Volume I written by Prudhvi Lal Bhukya and published by Springer Nature. This book was released on 2023-07-19 with total page 619 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first volume of the book-Emerging Human Viral diseases presents pathogenesis, diagnostics, and therapeutic strategies against viral respiratory and hemorrhagic fever diseases. The initial chapter discusses the underlying factors contributing to the emergence of these viral diseases. The subsequent chapters introduce structure, composition, and organization of viral genomes, and provide insight into function of important viral proteins. It also discusses the host factors that influence the outcome of viral infection and host immune response to the infections caused by these viruses. The book also discusses clinical manifestation and strategies for the prevention of virus diseases and highlights the recent technical advances in the diagnosis of virus diseases, prevention, and control strategies for viral infections. This timely book offers valuable resource for the scientists working in the field of emerging viral infections and those involved in preventing, controlling, and managing viral diseases.