Download or read book Analysis of P97 and P102 Paralogs of Mycoplasma Hyopneumoniae written by Cary Jon Adams and published by . This book was released on 2004 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mycoplasma hyopneumoniae, the causative agent of enzootic pneumonia in pigs, is an economically significant disease in the swine industry. Mycoplasmas cause a chronic infection characterized by high morbidity but low mortality. The disease has been implicated as an important agent in the porcine respiratory disease complex, which results in slower growth and decreased feed efficiency. M. hyopneumoniae colonizes the porcine respiratory tract by adherence to the cilia of ciliated epithelial cells. Recent studies have identified P97 as the major protein responsible for this adherence. The gene for P97 is located within a two gene operon that also includes the gene for P102. The function of P102 is unknown, but it is also thought to play an important role in adherence. The recent sequencing and annotation of the M. hyopneumoniae genome has identified several P97 and P102 paralogous genes, comprising two significant gene families. The goal of this study was to determine if these paralogs are expressed and if they could contribute to the adherence process. To accomplish this, we examined the paralogous gene expression patterns at the transcriptional and translational levels in high and low adherent phenotypic variants of M. hyopneumoniae strain 232, as well as in vivo grown organisms harvested from bronchoalveolar lavage fluids from mycoplasma-infected pigs. Transcriptional analysis of each paralog open reading frame was performed using reverse transcriptase-polymerase chain reaction assays. These results indicated the transcription of the paralogous genes between these isolates were variable and allowed us to identify several genes whose transcription might be responsive to changing environmental conditions. For translational analysis, monospecific, polyclonal antisera was produced in rabbits using a genetic vaccination approach against each of the paralogous family members. The design of the study allowed us to utilize unique protein sequences present on the carboxy terminus of each paralog as a gene-specific tag. Immunoblots against lysed, whole cell M. hyopneumoniae strain 232 antigen indicated that we were able to produce antibodies specific for paralogous gene products and demonstrated their translation into protein products.
Download or read book Mycoplasmas in Swine written by Dominiek Maes and published by CABI. This book was released on 2021-03-12 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Swine can be infected with many different mycoplasmas. Some are important pathogens, causing significant health and welfare issues in pigs and major losses to the swine industry worldwide. Other mycoplasmas are not pathogenic for swine and can be considered commensals. This book provides up-to-date scientific, clinical and practical information of the most important pathogenic mycoplasmas in swine. Most emphasis has been placed on Mycoplasma hyopneumoniae as the most economically important, but other pathogenic species like Mycoplasma hyorhinis, Mycoplasma hyosynoviae and Mycoplasma suis are also discussed. Written by internationally renowned scientists and clinicians from all over the world, this book draws together in depth knowledge, expertise and experience in swine mycoplasmas to provide an evidence-based, academically rigorous and practical collection. It aims to serve the scientific and veterinary community and the swine industry worldwide.
Download or read book Microbiology Australia written by and published by . This book was released on 2002-09 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Master s Theses Directories written by and published by . This book was released on 2004 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Education, arts and social sciences, natural and technical sciences in the United States and Canada".
Download or read book Immunopotentiation written by G. E. W. Wolstenholme and published by John Wiley & Sons. This book was released on 2009-09-16 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Novartis Foundation Series is a popular collection of the proceedings from Novartis Foundation Symposia, in which groups of leading scientists from a range of topics across biology, chemistry and medicine assembled to present papers and discuss results. The Novartis Foundation, originally known as the Ciba Foundation, is well known to scientists and clinicians around the world.
Download or read book Group A Streptococcus written by Thomas Proft and published by Humana. This book was released on 2021-06-03 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details Group A Streptococcus (GAS) research and provides the reader with an extensive collection of research protocols within this important field. Chapters guide readers through standard genetic protocols such as whole genome sequencing, transcriptome analyses, proteome analysis, application of GAS-derived molecular tools, and methods that are crucial for the evaluation of novel GAS vaccines and GAS virulence factors, including bactericidal assays and animal infection models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Group A Streptococcus: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Download or read book Biology of Salmonella written by Filipe Cabello and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Salmonella infections of man and animals continue to be a distressing health problem worldwide. Far from disappearing, the incidence of typhoid fever in developing countries may be far higher than we had imagined. Salmonella food poisoning has increased to one of the major causes of gastroenteritis in the developed world, in itself also an indication that animal salmonellosis is still a major cause for concern. The situation requires a concerted multidisciplinary research effort in order to generate the new information and technology needed to assist in the control of these diseases. This concept was the driving force behind the NATO Advanced Research Workshop on "Biology of Salmonella" held at Portorosa, Messina, Italy, May 11-15, 1992. With additional support from the University of Messina, Medeva Group Research (UK) and the Swiss Serum and Vaccine Research Institute, the meeting brought together epidemiologists, microbiologists, molecular biologists, immunologists and clinicians. All the participants were actively working on different but related aspects of Salmonella and salmonellosis, with most of the leading laboratories worldwide being represented. The workshop provided an excellent opportunity for interdisciplinary consultation; it is not often that the topic of Salmonella and salmonellosis is covered to such breadth and depth in one extended meeting. Keynote addresses by invited speakers were interspersed with offered papers, many by younger members of the scientific community, and this volume presents the collated manuscripts of the lectures and extended summaries of the offered papers.
