Download or read book A Study of an Oxidative Method for the Determination of Cystine in Proteins written by Jesse Maurice Berkowitz and published by . This book was released on 1952 with total page 62 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Measuring Oxidants and Oxidative Stress in Biological Systems written by Lawrence J. Berliner and published by Springer Nature. This book was released on 2020-08-08 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the methods of analysis and determination of oxidants and oxidative stress in biological systems. Reviews and protocols on select methods of analysis of ROS, RNS, oxygen, redox status, and oxidative stress in biological systems are described in detail. It is an essential resource for both novices and experts in the field of oxidant and oxidative stress biology.
Download or read book Techniques in Protein Chemistry III written by Ruth Hogue Angeletti and published by Academic Press. This book was released on 2014-06-28 with total page 563 pages. Available in PDF, EPUB and Kindle. Book excerpt: Techniques in Protein Chemistry III compiles papers presented at the Fifth Protein Society Symposium in Baltimore on June 22-26, 1991. This book discusses the protein and peptide recovery from PVDF membranes; high-sensitivity peptide mapping utilizing reversed-phase microbore and microcolumn liquid chromatography; and capillary electrophoresis for preparation of peptides and direct determination of amino acids. The TFMSA/TFA cleavage in t-Boc peptide synthesis; applications of automatic PTC amino acid analysis; and identification of O-glycosylation sites with a gas phase sequencer are also elaborated. This text likewise covers the conformational stability of the molten globule of cytochrome c and role of aqueous solvation in protein folding. This publication is useful to students and researchers interested in methods and research approaches on protein chemistry.
Download or read book Oxidative Folding of Proteins written by Matthias J Feige and published by Royal Society of Chemistry. This book was released on 2018-07-30 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: The formation of disulphide bonds is probably the most influential modification of proteins. These bonds are unique among post-translational modifications of proteins as they can covalently link cysteine residues far apart in the primary sequence of a protein. This has the potential to convey stability to otherwise marginally stable structures of proteins. However, the reactivity of cysteines comes at a price: the potential to form incorrect disulphide bonds, interfere with folding, or even cause aggregation. An elaborate set of cellular machinery exists to catalyze and guide this process: facilitating bond formation, inhibiting unwanted pairings and scrutinizing the outcomes. Only in recent years has it become clear how intimately connected this cellular machinery is with protein folding helpers, organellar redox balance and cellular homeostasis as a whole. This book comprehensively covers the basic principles of disulphide bond formation in proteins and describes the enzymes involved in the correct oxidative folding of cysteine-containing proteins. The biotechnological and pharmaceutical relevance of proteins, their variants and synthetic replicates is continuously increasing. Consequently this book is an invaluable resource for protein chemists involved in realted research and production.
Download or read book Oxidation of Amino Acids Peptides and Proteins written by Virender K. Sharma and published by John Wiley & Sons. This book was released on 2012-11-06 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: Explains the role of reactive intermediates in biological systems as well as in environmental remediation With its clear and systematic approach, this book examined the broad range of reactive intermediate that can be generated in biological environments, detailing the fundamental properties of each reactive intermediate. Readers gain a contemporary understanding of how these intermediates react with different compounds, with an emphasis on amino acids, peptides, and proteins. The author not only sets forth the basic chemistry and nature of reactive intermediates, he also demonstrates how the properties of the intermediates presented in the book compare with each other. Oxidation of Amino Acids, Peptides, and Proteins begins with a discussion of radical and non-radical reactive species as well as an exploration of the significance of reactive species in the atmosphere, disinfection processes, and environmental remediation. Next, the book covers such topics as: Thermodynamics of amino acids and reactive species and the effect of metal-ligand binding in oxidation chemistry Kinetics and mechanisms of reactive halogen, oxygen, nitrogen, carbon, sulfur and phosphate species as well as reactive high-valent Cr, Mn, and Fe species Reactivity of the species with molecules of biological and environmental importance Generation of reactive species in the laboratory for kinetics studies Oxidation of amino acids, peptides, and proteins by permanganate, ferryl, and ferrate species Application of reactive species in purifying water and treating wastewater With this book as their guide, readers will be able to assess the overall effects of reactive intermediates in biological environments. Moreover, they’ll learn how to apply this knowledge for successful water purification and wastewater treatment.
Download or read book A Laboratory Manual of Analytical Methods of Protein Chemistry written by P. Alexander and published by Elsevier. This book was released on 2014-05-09 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Laboratory Manual of Analytical Methods of Protein Chemistry, Volume 4 provides information pertinent to the fundamental aspects of protein chemistry. This book discusses the simple and accurate methods of estimating specific proteins. Organized into six chapters, this volume begins with an overview of the composition of acids and experimental conditions for the acid hydrolysis of proteins. This text then examines the advantages of high-voltage electrophoresis for amino acid analysis, which are paralleled by equal advantages in the peptide separation field. Other chapters consider the simple technique of estimating specific proteins, which is one of several based on the phenomenon of antigen-antibody precipitation in gels. This book discusses as well the summations of analyses in weight percentages of the various residues and of the nitrogen of each constituent. The final chapter deals with the electrical properties of molecules. This book is a valuable resource for physicists and research workers.
Download or read book Side Reactions in Peptide Synthesis written by Yi Yang and published by Academic Press. This book was released on 2015-09-01 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Side Reactions in Peptide Synthesis, based on the author's academic and industrial experience, and backed by a thorough review of the current literature, provides analysis of, and proposes solutions to, the most frequently encountered side reactions during peptide and peptidomimetic synthesis. This valuable handbook is ideal for research and process chemists working with peptide synthesis in diverse settings across academic, biotech, and pharmaceutical research and development. While peptide chemistry is increasingly prevalent, common side reactions and their causes are often poorly understood or anticipated, causing unnecessary waste of materials and delay. Each chapter discusses common side reactions through detailed chemical equations, proposed mechanisms (if any), theoretical background, and finally, a variety of possible solutions to avoid or alleviate the specified side reaction. - Provides a systematic examination on how to troubleshoot and minimize the most frequent side reactions in peptide synthesis - Gives chemists the background information and the practical tools they need to successfully troubleshoot and improve results - Includes optimization-oriented analysis of side reactions in peptide synthesis for improved industrial process development in peptidyl API (active pharmaceutical ingredient) production - Answers the growing, global need for improved, replicable processes to avoid impurities and maintain the integrity of the end product. - Presents a thorough discussion of critical factors in peptide synthesis which are often neglected or underestimated by chemists - Covers solid phase and solution phase methodologies, and provides abundant references for further exploration
Download or read book The Proteomics Protocols Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2007-10-09 with total page 969 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Download or read book Plant Cyclotides written by and published by Academic Press. This book was released on 2015-11-24 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Botanical Research publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. Currently in its 76th volume, the series features several reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. - Publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences - Contains commentary by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology, and ecology - This volume features reviews of the fast moving field of plant cyclotides
Download or read book Antioxidants in Sport Nutrition written by Manfred Lamprecht and published by CRC Press. This book was released on 2014-09-17 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of antioxidants in sports is controversial due to existing evidence that they both support and hinder athletic performance. Antioxidants in Sport Nutrition covers antioxidant use in the athlete ́s basic nutrition and discusses the controversies surrounding the usefulness of antioxidant supplementation. The book also stresses how antioxidants may affect immunity, health, and exercise performance. The book contains scientifically based chapters explaining the basic mechanisms of exercise-induced oxidative damage. Also covered are methodological approaches to assess the effectiveness of antioxidant treatment. Biomarkers are discussed as a method to estimate the bioefficacy of dietary/supplemental antioxidants in sports. This book is useful for sport nutrition scientists, physicians, exercise physiologists, product developers, sport practitioners, coaches, top athletes, and recreational athletes. In it, they will find objective information and practical guidance.
Download or read book Oxidants in Biology written by Giuseppe Valacchi and published by Springer Science & Business Media. This book was released on 2008-06-06 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: Oxidants, like other aspects of life, involves tradeoffs. Oxidants, whether intentionally produced or by-products of normal metabolism can either mediate a variety of critical biological processes but when present inappropriately cause extensive damage to biological molecules (DNA, proteins, and lipids). These effects can lead to either damage that is a major contributor to aging and degenerative diseases (or to other diseases such as cancer, cardiovascular disease, immune-system decline, brain dysfunction, and cataracts) or normal physiological function- tissue repair, defense against pathogens and cellular proliferation. On the other hand the body is equipped with a complex antioxidant/oxidant handling system which includes both enzymatic and nonenzymatic (i.e. small molecules such as flavonoids, ascorbate, tocopherol, and carotenoids) produced endogenously or derived from the diet. This book focuses on how the same molecules can have favorable or noxious effects depending on location, level and timing. Each chapter focuses on one particular molecule or oxidant/antioxidant system and provides a state of the art review of the current understanding regarding both positive and negative actions of the system under review.
Download or read book Methods for Protein Analysis written by Robert A. Copeland and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: As protein science continues to become an increasingly important aspect of academic and commercial sciences and technology, the need has arisen for a ready source of laboratory protocols for the analysis and evaluation of these biological polymers. Methods for Protein Analysis presents the methods most relevant to the generalist bench scientist working with proteins. A concise yet thorough summary, it covers laboratory methods that can be reasonably performed in a standard protein laboratory, without specialized equipment or expertise. Taking a how to approach, this book examines the techniques used to answer common protein analytical questions and describes methods useful in daily laboratory work. Methods for Protein Analysis is the ideal reference for protein laboratories in academic, government and industrial settings. It is an essential benchtop manual for first-year graduate students beginning their laboratory experience as well as for chemists, biochemists, and molecular biologists in the pharmaceutical, biotechnological, food and specialty chemical industries, and for analysts concerned with the purity and structural integrity of protein. Featuring illustrations and a convenient spiral binding, this guide offers a glossary of common abbreviations and a list of suppliers for protein science.
Download or read book Proteomic Applications in Biology written by Joshua Heazlewood and published by BoD – Books on Demand. This book was released on 2012-01-18 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has seen the field of proteomics expand from a highly technical endeavor to a widely utilized technique. The objective of this book is to highlight the ways in which proteomics is currently being employed to address issues in the biological sciences. Although there have been significant advances in techniques involving the utilization of proteomics in biology, fundamental approaches involving basic sample visualization and protein identification still represent the principle techniques used by the vast majority of researchers to solve problems in biology. The work presented in this book extends from overviews of proteomics in specific biological subject areas to novel studies that have employed a proteomics-based approach. Collectively they demonstrate the power of established and developing proteomic techniques to characterize complex biological systems.
Download or read book The Role of Protein and Amino Acids in Sustaining and Enhancing Performance written by Institute of Medicine and published by National Academies Press. This book was released on 1999-09-15 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a commonly held belief that athletes, particularly body builders, have greater requirements for dietary protein than sedentary individuals. However, the evidence in support of this contention is controversial. This book is the latest in a series of publications designed to inform both civilian and military scientists and personnel about issues related to nutrition and military service. Among the many other stressors they experience, soldiers face unique nutritional demands during combat. Of particular concern is the role that dietary protein might play in controlling muscle mass and strength, response to injury and infection, and cognitive performance. The first part of the book contains the committee's summary of the workshop, responses to the Army's questions, conclusions, and recommendations. The remainder of the book contains papers contributed by speakers at the workshop on such topics as, the effects of aging and hormones on regulation of muscle mass and function, alterations in protein metabolism due to the stress of injury or infection, the role of individual amino acids, the components of proteins, as neurotransmitters, hormones, and modulators of various physiological processes, and the efficacy and safety considerations associated with dietary supplements aimed at enhancing performance.
Download or read book Protein Sequence Determination written by Saul B. Needleman and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 363 pages. Available in PDF, EPUB and Kindle. Book excerpt: "All the King's horses and all the King's mm couldn't put Humpty Dumpty together again." It is entirely possible that the difficulty facing "all the King's men" was principally the lack of a sufficient guide to the techniques of reassembling from a series of small components, the original entity. It is the sincere hope of the editor of the present work and of each of the contributing authors that the modern researcher will not face a similar predicament in his endeavours to reconstruct the complete primary sequence of a protein from the array of component amino acids. Rather, it is the intent that, with this volume, he may proceed untimorously if not with outright confidence toward achieving his purpose. To the newcomer in protein sequencing, compelled by necessity, or fascination, to investigate the exact order of amino acids in proteins, the question of "where to begin" - or "how to do it" is urgent. To those more skilled, a ready source of additional techniques should nevertheless be of value. This volume attempts to present in a single source a discussion of the methods and techniques useful to the determination of the primary structures of proteins and peptides. Hopefully, this book will tell the reader "how to do it".
Download or read book Nutrient Metabolism written by Martin Kohlmeier and published by Elsevier. This book was released on 2003-10-01 with total page 841 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nutrient Metabolism defines the molecular fate of nutrients and other dietary compounds in humans, as well as outlining the molecular basis of processes supporting nutrition, such as chemical sensing and appetite control. It focuses on the presentation of nutritional biochemistry; and the reader is given a clear and specific perspective on the events that control utilization of dietary compounds. Slightly over 100 self-contained chapters cover all essential and important nutrients as well as many other dietary compounds with relevance for human health. An essential read for healthcare professionals and researchers in all areas of health and nutrition who want to access the wealth of nutrition knowledge available today in one single source.Key Features* Highly illustrated with relevant chemical structures and metabolic pathways* Foreword by Steven Zeisel, Editor-in-chief of the Journal of Nutritional Biochemistry* First comprehensive work on the subject
Download or read book Bioconjugate Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 2010-07-26 with total page 1233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions
