Download or read book A Low Cost Approach to PCR written by Eva Harris and published by Oxford University Press. This book was released on 1998-12-31 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: The polymerase chain reaction (PCR) is a technique used to replicate specific pieces of DNA millions of times, which permits the detection and analysis of minute amounts of nucleic acids. Since its introduction in the late 1980s, this technique has been applied not only in molecular biology research but also in fields as diverse as anthropology, phylogeny, and forensics. However, despite the large impact of PCR, many of its applications remain within the confines of research and the academic environment. Now, in A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques, Dr. Eva Harris makes this elegantly simple technique more accessible to researchers, physicians, and laboratory workers throughout the world. She provides a description of the theoretical basis of the technique, the practical details of the method, and the philosophy behind the technology transfer program that she developed over the last ten years. The book serves as a guide for potential users in developing countries and for scientists in developed countries who may wish to work abroad. In addition, the low-cost approach outlined in this book can be useful for high school, undergraduate, or continuing education programs in the United States. While the specific applications of PCR outlined in the book are immediately useful to the study of infectious diseases, the approach presented can be generalized to a number of other technologies and situations. The book will help laboratories in many areas of the world generate information on site for use by physicians, epidemiologists, public health workers, and health policy professionals to develop new strategies for disease control.
Download or read book A Low Cost Approach to PCR written by Eva Harris and published by Oxford University Press. This book was released on 1998-12-31 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: The polymerase chain reaction (PCR) is a technique used to replicate specific pieces of DNA millions of times, which permits the detection and analysis of minute amounts of nucleic acids. Since its introduction in the late 1980s, this technique has been applied not only in molecular biology research but also in fields as diverse as anthropology, phylogeny, and forensics. However, despite the large impact of PCR, many of its applications remain within the confines of research and the academic environment. Now, in A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques, Dr. Eva Harris makes this elegantly simple technique more accessible to researchers, physicians, and laboratory workers throughout the world. She provides a description of the theoretical basis of the technique, the practical details of the method, and the philosophy behind the technology transfer program that she developed over the last ten years. The book serves as a guide for potential users in developing countries and for scientists in developed countries who may wish to work abroad. In addition, the low-cost approach outlined in this book can be useful for high school, undergraduate, or continuing education programs in the United States. While the specific applications of PCR outlined in the book are immediately useful to the study of infectious diseases, the approach presented can be generalized to a number of other technologies and situations. The book will help laboratories in many areas of the world generate information on site for use by physicians, epidemiologists, public health workers, and health policy professionals to develop new strategies for disease control.
Download or read book Bennett Brachman s Hospital Infections written by William R. Jarvis and published by Lippincott Williams & Wilkins. This book was released on 2013-10-17 with total page 1339 pages. Available in PDF, EPUB and Kindle. Book excerpt: For over three decades, Bennett & Brachman’s Hospital Infections has been a respected and influential resource in the prevention and control of healthcare-associated infections (HAIs). Now in its Sixth Edition, the book continues to provide readers with the latest information in the field of healthcare epidemiology, infection control, patient safety, and the prevention and control of HAIs. Many of the current contributors are or were employed by or trained at the Centers for Disease Control and Prevention (CDC) and have a thorough knowledge of healthcare epidemiology. Topics covered include HAI epidemiology; surveillance; control programs; antimicrobial stewardship;antimicrobial resistance; mechanisms of resistance; sterilization and disinfection; food-borne diseases; the role of the laboratory, intensive care unit, operating room, dialysis, and nursery settings; and specific hospital-acquired infections.
Download or read book Biotechnological Approaches in Food Adulterants written by Madan L Verma and published by CRC Press. This book was released on 2020-11-18 with total page 307 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book highlights the biotechnological advancement in the area of food adulterants and outlines the current state of art technologies in the detection of food adulterants using omics and nanobiotechnology. The book provides insights to the most recent innovations, trends, concerns, and challenges in food adulterants. It identifies key research topics and practical applications of modern cutting-edge technologies employed for detection of food adulterants including: expansion of food adulterants market, potential toxicity of food adulterants and the prevention of food adulteration act, cutting-edge technology for food adulterants detection, and biosensing and nanobiosensing based detection of food adulterants. There is need for new resources in omics technologies for the application of new nanobiotechnology. Biotechnological Approaches in Food Adulterants provides an overview of the contributions of food safety and the most up-to-date advances in omics and nanobiotechnology approaches to a diverse audience from postgraduate students to researchers in biochemical engineering, biotechnology, food technologist, environmental technologists, and pharmaceutical professionals.
Download or read book Animal Biotechnology written by Ashish S. Verma and published by Academic Press. This book was released on 2020-06-11 with total page 799 pages. Available in PDF, EPUB and Kindle. Book excerpt: Animal Biotechnology: Models in Discovery and Translation, Second Edition, provides a helpful guide to anyone seeking a thorough review of animal biotechnology and its application to human disease and welfare. This updated edition covers vital fundamentals, including animal cell cultures, genome sequencing analysis, epigenetics and animal models, gene expression, and ethics and safety concerns, along with in-depth examples of implications for human health and prospects for the future. New chapters cover animal biotechnology as applied to various disease types and research areas, including in vitro fertilization, human embryonic stem cell research, biosensors, enteric diseases, biopharming, organ transplantation, tuberculosis, neurodegenerative disorders, and more. - Highlights the latest biomedical applications of genetically modified and cloned animals, with a focus on cancer and infectious diseases - Offers first-hand accounts of the use of biotechnology tools, including molecular markers, stem cells, animal cultures, tissue engineering, ADME and CAM Assay - Includes case studies that illustrate safety assessment issues, ethical considerations, and intellectual property rights associated with the translation of animal biotechnology studies
Download or read book Basic Science Methods for Clinical Researchers written by Morteza Jalali and published by Academic Press. This book was released on 2017-03-31 with total page 383 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Science Methods for Clinical Researchers addresses the specific challenges faced by clinicians without a conventional science background. The aim of the book is to introduce the reader to core experimental methods commonly used to answer questions in basic science research and to outline their relative strengths and limitations in generating conclusive data. This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow's clinician scientists and future leaders in discovery science. - Serves as a helpful guide for clinical researchers who lack a conventional science background - Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms - Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data - Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
Download or read book Practical Healthcare Epidemiology written by Ebbing Lautenbach and published by Cambridge University Press. This book was released on 2018-04-19 with total page 455 pages. Available in PDF, EPUB and Kindle. Book excerpt: A clear, hands-on outline of best practices for infection prevention that directly improve patient outcomes across the healthcare continuum.
Download or read book Development of Practical Methods to Assess the Presence of Bacterial Pathogens in Water written by W. A. Yanko and published by IWA Publishing. This book was released on 2004-03-01 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: The objective of this project was to develop and evaluate a practical technique to indicate the probability of the presence of bacterial pathogens in receiving waters. A practical method was defined as one that would limit the use of defined culture-based microbiological methods, and would be based on: (1) validating indicator organisms that predicted the presence of pathogens, or (2) detection of pathogens by polymerase chain reaction (PCR)-based assays. The study also assessed the utility of PCR-based technology for bacterial pathogen detection with respect to technology transfer to a wider range of water and wastewater facilities.
Download or read book Present Knowledge in Food Safety written by Michael E. Knowles and published by Academic Press. This book was released on 2022-10-08 with total page 1190 pages. Available in PDF, EPUB and Kindle. Book excerpt: Present Knowledge in Food Safety: A Risk-Based Approach Through the Food Chain presents approaches for exposure-led risk assessment and the management of changes in the chemical, pathogenic microbiological and physical (radioactivity) contamination of 'food' at all key stages of production, from farm to consumption. This single volume resource introduces scientific advances at all stages of the production to improve reliability, predictability and relevance of food safety assessments for the protection of public health. This book is aimed at a diverse audience, including graduate and post-graduate students in food science, toxicology, microbiology, medicine, public health, and related fields. The book's reach also includes government agencies, industrial scientists, and policymakers involved in food risk analysis. - Includes new technologies such as nanotechnology, genetic modification, and cloning - Provides information on advances in pathogen risk assessment through novel and real-time molecular biological techniques, biomarkers, resistance measurement, and cell-to-cell communication in the gut - Covers the role of the microbiome and the use of surrogates (especially for viruses)
Download or read book PCR Protocols written by Michael A. Innis and published by Academic Press. This book was released on 2012-12-02 with total page 501 pages. Available in PDF, EPUB and Kindle. Book excerpt: The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual. - Avoid contamination--with specific instructions on setting up your lab - Avoid cumbersome molecular biological techniques - Discover new applications
Download or read book Human Biology written by Raymond Pearl and published by . This book was released on 2000 with total page 690 pages. Available in PDF, EPUB and Kindle. Book excerpt: Includes section "Recent literature useful in the study of human biology."
Download or read book Polymerase Chain Reaction written by Mark A. Behlke and published by . This book was released on 2019-07 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: This indispensable manual is a compilation of review articles written by experts in the field of PCR technology. It is a recommended purchase for all microbiology and molecular biology laboratories and university libraries.
Download or read book Acinetobacter written by Eugénie Bergogne-Bérézin and published by Springer Science & Business Media. This book was released on 2008-12-19 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is currently increasing interest concerning the biology and disease caused by Acinetobacter species. Such interest, however, developed relatively slowly because of the necessity to clarify the confusing taxonomy of these organisms. Much work was needed to identify various species as members of this genus, to recognize their epidemiologic profile, their pathogenic role and their increasing importance as multi-antibiotic resistant organisms. In recent years improvement of genetic approaches, recognition of plasmids, integrons and chromosomal sources of resistance mechanisms aroused interest on the role of Acinetobacters in disease by many microbiologists and clinicians, especially internists and infectious disease specialists. In this regard, physicians are frequently confronted with extremely difficult therapeutic approaches for treatment and prevention of severe nosocomial infections due to multi antibiotic resistant Acinetobacter. Moreover, recent observations of community acquired infections have been reported, especially in patients with various risk factors such as immuno-deficiencies. Also, it is now becoming evident that Acinetobacter infections occur frequently in violent situations such as earthquake or war zones. The mechanisms of Acinetobacter virulence are becoming increasingly clear, providing new insights into their pathogenic role in community acquired infections. It is apparent the time is appropriate for detailed review of the increasing knowledge concerning important new information, both clinical and therapeutic, especially information concerning virulence, resistance mechanisms and typing of Acinetobacter spp. Many new findings are accumulating in almost an exponential manner since publication of previous books on this subject in 1991 and 1996.
Download or read book Rapid Cycle Real Time PCR written by S. Meuer and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first comprehensive treatise on Rapid Cycle Real-Time PCR. With amplification times of 15-30 minutes of on-line detection and analysis, nucleic acid quantification of mutation analysis finally becomes a routine, powerful and rapid method. Focusing primarily on the LightCycler, an instrument that combines Rapid Cycle PCR with fluorescent monitoring, this technology provides convenient analysis by melting temperatures. PCR products can be identified by product Tm, and single base mismatches can easily be genotyped by probe Tm. Methods chapters detail the theory behind quantification of mutation analysis; the design of synthesis of fluorescent hybridization probes of the preparation of template DNA. Application chapters apply nucleid acid quantification to infectious organisms of intracellular messengers and mutation detection to somatic of acquired mutations.
Download or read book Principles and Technical Aspects of PCR Amplification written by Elizabeth van Pelt-Verkuil and published by Springer Science & Business Media. This book was released on 2008-03-14 with total page 333 pages. Available in PDF, EPUB and Kindle. Book excerpt: Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).
Download or read book Gene Quantification written by Francois Ferre and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 379 pages. Available in PDF, EPUB and Kindle. Book excerpt: Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.
Download or read book Reducing the Impact of Poverty on Health and Human Development written by Stephen G. Kaler and published by Wiley-Blackwell. This book was released on 2008-07-08 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work examines the many ways in which poverty contributes to ill health among the poor and to impeded development among poor children. The volume is a response to a request by the Council of Science Editors that journals all over the world publish an issue in October 2007 addressing the theme of poverty and human development.
