Download or read book Whole Cell Biocatalysis written by Sudheer D. V. N. Pamidimarri and published by CRC Press. This book was released on 2024-06-28 with total page 673 pages. Available in PDF, EPUB and Kindle. Book excerpt: Whole-cell biocatalysis is a promising and emerging field of biological science that is mostly based on advancements in biosystems engineering for the production of useful products such as chemicals, fuels, and pharmaceuticals. It is a unique platform that provides an efficient catalytic system at affordable cost and with low maintenance. Recent studies have proven that whole-cell catalysis is very useful in conducting many complex and complicated chemical reactions that could be executed with greater ease and in an eco-friendly manner in ambient conditions with zero/minimal ecological effect. This has made whole-cell biocatalysis very popular and a center of significance in the field of modern biological catalysis. This new volume offers a comprehensive understanding of whole-cell biocatalysis, beginning with an introduction and an overview of relevant tools and techniques. It presents the basic as well as the advanced concepts of whole-cell biocatalysis, thus acting as a complete guide for the reader. It looks at the promising developments that have taken place in the past decade and discusses the application of computational tools, the use of metabolic flux systems, and the role of metabolic networks for the cell system development for whole-cell catalysis. The chapters explore whole-cell biocatalysis for the production of chemicals, of fuels, and for environmental restoration, with case studies and success stories that showcase new developments in the technology. This volume provides a valuable presentation of whole-cell biocatalysis for graduate students, faculty, industry professionals, and others working in biochemistry and bio-systems engineering.
Download or read book Immobilization of Enzymes and Cells written by Jose M. Guisan and published by Humana. This book was released on 2021-01-29 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.
Download or read book Applied Biocatalysis written by John Whittall and published by John Wiley & Sons. This book was released on 2020-08-21 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides clear and comprehensive coverage of recently developed applied biocatalysis for synthetic organic chemists with an emphasis to promote green chemistry in pharmaceutical and process chemistry This book aims to make biocatalysis more accessible to both academic and industrial synthetic organic chemists. It focuses on current topics within the applied industrial biocatalysis field and includes short but detailed experimental methods on timely novel biocatalytic transformations using new enzymes or new methodologies using known enzymes. The book also features reactions that are “expanding and making the enzyme toolbox available to chemists”—providing readers with comprehensive methodology and detailed key sourcing information of a wide range of enzymes. Chapters in Applied Biocatalysis: The Chemist’s Enzyme Toolkit are organized by reaction type and feature a short introductory section describing the current state of the art for each example. Much of the book focuses on processes for which the enzymes are readily available so that organic chemists can synthesize appropriate quantities of chemicals with available materials in a standard chemical laboratory. Advanced methods are included to present examples of new enzymes that might encourage collaboration with suppliers or academic groups and that will educate chemists of rapidly expanding future possibilities. Focuses on current topics within the applied industrial biocatalysis field Offers experimental methods on novel biocatalytic transformations using new enzymes or new methodology using known enzymes Covers the hot topics of enzyme and chemoenzymatic cascades and biocatalysis in flow Edited by noted experts from both academia and industry with years of experience in the field of biocatalysis—particularly, the industrial applications of enzymes Written for synthetic organic chemists working in all industries but especially the pharmaceutical industry and for those in academia with an eye for biocatalysis, Applied Biocatalysis: The Chemist’s Enzyme Toolkit will also benefit academic groups in chemistry and related sciences that are using enzymes for synthetic purposes, as well as those working in the area of enzymology and molecular biology.
Download or read book Practical Methods for Biocatalysis and Biotransformations 2 written by John Whittall and published by John Wiley & Sons. This book was released on 2012-04-25 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities. Practical Methods for Biocatalysis and Biotransformations 2 is a "how-to" guide that focuses on the practical applications of enzymes and strains of microorganisms that are readily obtained or derived from culture collections. The sources of starting materials and reagents, hints, tips and safety advice (where appropriate) are given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology are given and relevant references to the primary literature are cited. This second volume – which can be used on its own or in combination with the first volume - concentrates on new applications and new enzyme families reported since the first volume. Contents include: introduction to recent developments and future needs in biocatalysts and synthetic biology in industry reductive amination enoate reductases for reduction of electron deficient alkenes industrial carbonyl reduction regio- and stereo- selective hydroxylation oxidation of alcohols selective oxidation industrial hydrolases and related enzymes transferases for alkylation, glycosylation and phosphorylation C-C bond formation and decarboxylation halogenation/dehalogenation/heteroatom oxidation tandem and sequential multi-enzymatic syntheses Practical Methods for Biocatalysis and Biotransformations 2 is an essential collection of biocatalytic methods for chemical synthesis which will find a place on the bookshelves of synthetic organic chemists, pharmaceutical chemists, and process R&D chemists in industry and academia.
Download or read book Enzyme or Whole Cell Immobilization for Efficient Biocatalysis Focusing on Novel Supporting Platforms and Immobilization Techniques written by Wen-Yong Lou and published by Frontiers Media SA. This book was released on 2021-04-21 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Oxidation of Cyclohexane written by I. V. Berezin and published by Elsevier. This book was released on 2018-04-12 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Oxidation of Cyclohexane focuses on the processes, methodologies, reactions, and approaches involved in the oxidation of cyclohexane. The publication first offers information on the theory of slow chain oxidations and the products of liquid-phase cyclohexane oxidation. Discussions focus on the applicability of the stationary state method to liquid-phase oxidation reactions; mechanism of liquid hydrocarbon chain oxidation; kinetic equations for product accumulation in degenerate branching chain reactions; and changes of the volume of the liquid phase due to oxidation product formation. The text then ponders on experimental apparatus for the study of the liquid-phase oxidation of cyclohexane, including prevention of cyclohexane losses in the waste gases, explosion danger and problems of safety, and characteristics of gas sampling in cyclohexane oxidation apparatus. The manuscript takes a look at the kinetics of uncatalyzed cyclohexane oxidation and kinetics of cyclohexane oxidation in continuous flow systems. Topics include effect of temperature on the relative yield of cyclohexane oxidation products; kinetics of cyclohexane oxidation in a glass reactor; rate of oxygen absorption and accumulation of reaction products; ideal displacement reactor; and determination of diffusion factor. The publication is a dependable reference for readers interested in the oxidation of cyclohexane.
Download or read book Flow Chemistry written by Santiago V Luis and published by Royal Society of Chemistry. This book was released on 2019-10-18 with total page 560 pages. Available in PDF, EPUB and Kindle. Book excerpt: In flow chemistry reactions are performed in a reactor with the reactants pumped through it. It has the benefit of being easily scaled up and it is straightforward to integrate synthesis, workup and analysis into one system. This volume provides an update on recent advances in the field of flow chemistry, with special emphasis on new, integrated approaches for green and efficient chemistry. This book is a valuable resource for researchers in green chemistry, chemical engineers and Industrial chemists working in the pharmaceutical and fine chemicals industries.
Download or read book Novel Enzyme and Whole Cell Biocatalysts written by Anwar Sunna and published by MDPI. This book was released on 2020-11-04 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: The concept of a circular economy relies on waste reduction, valorization, and recycling. Global trends for “green” synthesis of chemicals have positioned the field of enzyme technology and biocatalysis (multi-enzymes and whole-cells) as an alternative for the synthesis of more social- and environmentally-responsible bio-based chemicals. Recent advances in synthetic biology, computational tools, and metabolic engineering have supported the discovery of new enzymes and the rational design of whole-cell biocatalysts. In this book, we highlight these current advances in the field of biocatalysis, with special emphasis on novel enzymes and whole-cell biocatalysts for applications in several industrial biotechnological applications.
Download or read book Biocatalysts and Enzyme Technology written by Klaus Buchholz and published by John Wiley & Sons. This book was released on 2012-12-21 with total page 609 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition of a bestselling textbook offers an instructive and comprehensive overview of our current knowledge of biocatalysis and enzyme technology. The book now contains about 40% more printed content. Three chapters are completely new, while the others have been thoroughly updated, and a section with problems and solutions as well as new case studies have been added. Following an introduction to the history of enzyme applications, the text goes on to cover in depth enzyme mechanisms and kinetics, production, recovery, characterization and design by protein engineering. The authors treat a broad range of applications of soluble and immobilized biocatalysts, including wholecell systems, the use of non-aqueous reaction systems, applications in organic synthesis, bioreactor design and reaction engineering. Methods to estimate the sustainability, important internet resources and their evaluation, and legislation concerning the use of biocatalysts are also covered.
Download or read book Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives written by Jesús Fernández Lucas and published by John Wiley & Sons. This book was released on 2019-04-29 with total page 348 pages. Available in PDF, EPUB and Kindle. Book excerpt: A review of innovative tools for creative nucleic acid chemists that open the door to novel probes and therapeutic agents Nucleic acids continue to gain importance as novel diagnostic and therapeutic agents. With contributions from noted scientists and scholars, Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives is a practical reference that includes a wide range of approaches for the synthesis of designer nucleic acids and their derivatives. The book covers enzymatic (including chemo-enzymatic) methods, with a focus on the synthesis and incorporation of modified nucleosides. The authors also offer a review of innovative approaches for the non-enzymatic chemical synthesis of nucleic acids and their analogs and derivatives, highlighting especially challenging species. The book offers a concise review of the methods that prepare novel and heavily modified polynucleotides in sufficient amount and purity for most clinical and research applications. This important book: -Presents a timely and topical guide to the synthesis of designer nucleic acids and their derivatives -Addresses the growing market for nucleotide-derived pharmaceuticals used as anti-infectives and chemotherapeutic agents, as well as fungicides and other agrochemicals. -Covers novel methods and the most recent trends in the field -Contains contributions from an international panel of noted scientistics Written for biochemists, medicinal chemists, natural products chemists, organic chemists, and biotechnologists, Enzymatic and Chemical Synthesis of Nucleic Acid Derivatives is a practice-oriented guide that reviews innovative methods for the enzymatic as well as non-enzymatic synthesis of nucleic acid species.
Download or read book Immobilization of Enzymes and Cells written by Jose M. Guisan and published by Humana. This book was released on 2016-08-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.
Download or read book Biocatalysis for Practitioners written by Gonzalo de Gonzalo and published by John Wiley & Sons. This book was released on 2021-07-19 with total page 54 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference book originates from the interdisciplinary research cooperation between academia and industry. In three distinct parts, latest results from basic research on stable enzymes are explained and brought into context with possible industrial applications. Downstream processing technology as well as biocatalytic and biotechnological production processes from global players display the enormous potential of biocatalysts. Application of "extreme" reaction conditions (i.e. unconventional, such as high temperature, pressure, and pH value) - biocatalysts are normally used within a well defined process window - leads to novel synthetic effects. Both novel enzyme systems and the synthetic routes in which they can be applied are made accessible to the reader. In addition, the complementary innovative process technology under unconventional conditions is highlighted by latest examples from biotech industry.
Download or read book Applied Biocatalysis written by Adrie J.J. Straathof and published by CRC Press. This book was released on 2003-09-02 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the essential steps in the development of biocatalytic processes from concept to completion. It is a carefully integrated text which combines the fundamentals of biocatalysis with technological experience and in-depth commercial case studies. The book starts with an introductory look at the characteristics and present applications of biocatalysts, followed by more detailed overviews of these areas.
Download or read book Biocatalysis written by Peter Grunwald and published by Imperial College Press. This book was released on 2009 with total page 1052 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book covers the fundamentals of the field of biocatalysis that are not treated in such detail (or even not at all) in existing biocatalysis books or biochemistry textbooks. It of course does not substitute existing biochemistry textbooks but will serve a suitable supplement as it discusses biochemical fundamentals in connection with the respective topics.With focus on the interdisciplinary nature of biocatalysis, the book contains many aspects of fundamental organic chemistry and some of inorganic chemistry as well, which should make it interesting not only for biochemistry but also for chemistry students. An important theme being emphasized in the book is that applied biocatalysis is one of the main prerequisites for a sustainable development.The topics covered ranges from basic enzyme chemistry (biosynthesis, structure, properties, interaction forces, kinetics) to a detailed description of catalytic mechanisms. It covers the fundamentals of the different enzyme classes together with their applications in native and in immobilized state or in the form of whole cells in aqueous as well as non-conventional media. Topics such as catalytic antibodies, nucleic acid catalysts, non-ribosomal peptide synthesis, evolutionary methods, and the design of cells are also included.
Download or read book Introduction to Biocatalysis Using Enzymes and Microorganisms written by S. M. Roberts and published by Cambridge University Press. This book was released on 1995-01-27 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book gives an introduction to biotransformations, the practice of harnessing biological catalysts for the preparation of useful chemicals.
Download or read book Biocatalysis for Green Chemistry and Chemical Process Development written by Junhua (Alex) Tao and published by John Wiley & Sons. This book was released on 2011-06-09 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes recent progress in enzyme-driven green syntheses of industrially important molecules. The first three introductory chapters overview recent technological advances in enzymes and cell-based transformations, and green chemistry metrics for synthetic efficiency. The remaining chapters are directed to case studies in biotechnological production of pharmaceuticals (small molecules, natural products and biologics), flavors, fragrance and cosmetics, fine chemicals, value-added chemicals from glucose and biomass, and polymeric materials. The book is aimed to facilitate the industrial applications of this powerful and emerging green technology, and catalyze the advancement of the technology itself.
Download or read book Practical Methods for Biocatalysis and Biotransformations 3 written by John Whittall and published by John Wiley & Sons. This book was released on 2016-02-16 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities. Practical Methods for Biocatalysis and Biotransformations 3 will be a companion book to Practical Methods for Biocatalysis and Biotransformations (2009) and Practical Methods for Biocatalysis and Biotransformations 2 (2012). Following the successful format of the two volumes, it will be a “how-to” guide focusing on commercially available enzymes and strains of microorganisms that are readily obtained from culture collections. The source of starting materials and reagents, hints, tips and safety advice (where appropriate) will be given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology will be given and relevant references to the primary literature will be cited. Contents include: Biotransformation Process Technology Industrial Biooxidation Hydrolase catalysed hydrolysis/synthesis Reduction Oxidation Halogenation Transferase catalysed glycosylation, methylation, etc C-C bond formation Tandem Biocatalytic Reactions Practical Methods for Biocatalysis and Biotransformations, Volume 3 is an essential collection of validated biocatalytic methods which will find a place on the bookshelves of synthetic organic chemists, pharmaceutical chemists, and process R&D chemists in industry and academia.
