Download or read book Whole brain Voltage Imaging in Larval Zebrafish Using Light field Microscopy written by Zeguan Wang and published by . This book was released on 2020 with total page 68 pages. Available in PDF, EPUB and Kindle. Book excerpt: Monitoring pan-neuronal electrical activity in awake vertebrates is a yet-to-be-achieved goal in neuroscience that will facilitate our efforts to understand how the brain processes information and mediates behaviors. Here we present Sparse Decomposition Light-Field Microscopy (SDLFM), a light-field microscopy method with improved resolution for volumetric neural activity imaging, and computational and experimental evaluations of its capacity for whole-brain voltage imaging in larval zebrafish. The work in this thesis includes three parts. First, we designed and implemented SDLFM and demonstrated its cellular-resolution ability in larval zebrafish expressing pan-neuronal GCaMP6. Second, we simulated the voltage imaging and the computation processes of SDLFM for zebrafish brains and found that in order to achieve a >0.9 correlation coefficient between the extracted SDLFM signals and the real voltage signals with our current hardware and voltage indicators, the proportion of the neurons randomly labeled with fluorescence in a zebrafish brain must be reduced to below 20%. Finally, we imaged live brains of zebrafish larvae expressing pan-neuronal Voltron525-ST and transient SomArchon at a volume rate of 307 Hz with a volumetric field-of-view of ~700 [mu]mx~400 [mu]mx~300 [mu]m. Although spontaneous spikes were traced in these recordings, we speculate some signals might result from noise and artifacts. In summary, we have developed SDLFM, which allows high-speed whole-brain imaging at cellular resolution in larval zebrafish. New camera sensors with higher quantum efficiency and larger dynamic range, and new voltage indicators with increased brightness and sensitivity are needed to achieve the signal-to-noise ratio required to record the voltage activity from a densely labeled fish brain.
Download or read book Imaging Manipulation and Optogenetics in Zebrafish written by Itia Amandine Favre‐Bulle and published by Springer. This book was released on 2018-08-12 with total page 86 pages. Available in PDF, EPUB and Kindle. Book excerpt: The work described here investigates the advantages and limitations of using laser light for the deep in-vivo illumination and micromanipulation of the neuronal system in zebrafish. To do so, it combines and develops novel optical methods such as optogenetics, light sheet microscopy and optical micromanipulation. It also demonstrates, for the first time, that directional and focused laser beams can successfully be used to target large objects at considerable depth in a living organism to exert purely optical force – in this case on otoliths (ear stones) – and create fictive vestibular stimuli in a stationary animal. The behavioural study and simultaneous imaging of the whole brain reveal the location of the brain cells specific to each ear stone. Elucidating these fundamental neural processes holds substantial value for basic neuroscience researchers, who still have only a vague grasp of how brain circuits mediate perception. As such, it represents highly innovative research that has already led to high-impact publications and is now being intensively pursued.
Download or read book The world according to zebrafish How neural circuits generate behaviour written by Gonzalo G. De Polavieja and published by Frontiers E-books. This book was released on 2014-11-07 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding how the brain functions is one of the most ambitious current scientific goals. This challenge will only be accomplish by a multidisciplinary approach involving genetics, molecular biology, optics, ethology, neurobiology and mathematics and using tractable model systems. The zebrafish larva is a transparent genetically tractable small vertebrate, ideal for the combination state-of-the- art imaging techniques (e.g. two-photon scanning microscopy, single-plane illumination microscopy, spatial light modulator microscopy and lightfield microscopy), bioluminiscence and optogenetics to monitor and manipulate neuronal activity from single specific neurons up to the entire brain, in an intact behaving organism. Furthermore, the zebrafish model offers large and increasing collection of mutant and transgenic lines modelling human brain diseases. With these advantages in hand, the zebrafish larva became in the recent years, a novel animal model to study neuronal circuits and behaviour, taking us closer than ever before to understand how the brain controls behaviour.
Download or read book Development of optical methods for real time whole brain functional imaging of zebrafish neuronal activity written by Lapo Turrini and published by Firenze University Press. This book was released on 2020 with total page 202 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Neural Substrate of Goal directed Locomotion in Zebrafish and Whole brain Functional Imaging with Two photon Light sheet Microscopy written by Sébastien Wolf and published by . This book was released on 2017 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first part of this thesis presents an historical overview of neural recording techniques, followed by a study on the development of a new imaging method for zebrafish neural recording: two-photon light sheet microscopy. Combining the advantages of two-photon point scanning microscopy and light sheet techniques, the two-photon light sheet microscope warrants a high acquisition speed with low photodamage and allows to circumvent the main limitation of one-photon light sheet microscopy: the disturbance of the visual system. The second part of the thesis is focused on goal-directed navigation in zebrafish larvae. After an exhaustive review on chemotaxis, phototaxis and thermotaxis in various animal models, we report a study that reveals the neural computation underlying phototaxis in zebrafish. Combining virtual-reality behavioral assays, volumetric calcium recordings, optogenetic stimulation, and circuit modeling, this work shows that a self-oscillating hindbrain population called the hindbrain oscillator (HBO) acts as a pacemaker for ocular saccades, controls the orientation of successive swim-bouts during zebrafish larva navigation, and is responsive to light in a state-dependent manner such that its response to visual inputs varies with the motor context. This peculiar response to visual inputs biases the fish trajectory towards brighter regions (phototaxis). The third part provides a discussion on the neural basis of ocular saccades in vertebrates. We conclude with some recent preliminary results on heat perception in zebrafish suggesting that the same hindbrain circuit may be at play in thermotaxis as well.
Download or read book Imaging 3D Visually evoked Calcium Dynamics in Larval Zebrafish Optic Tectum Using Light Field Microscopy written by Todd Anderson and published by . This book was released on 2009 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Development of Optical Methods for Realtime Whole brain Functional Imaging of Zebrafish Neuronal Activity written by Lapo Turrini and published by . This book was released on 2020 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Quantum Computing For The Brain written by Melanie Swan and published by World Scientific. This book was released on 2022-05-30 with total page 551 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantum Computing for the Brain argues that the brain is the killer application for quantum computing. No other system is as complex, as multidimensional in time and space, as dynamic, as less well-understood, as of peak interest, and as in need of three-dimensional modeling as it functions in real-life, as the brain.Quantum computing has emerged as a platform suited to contemporary data processing needs, surpassing classical computing and supercomputing. This book shows how quantum computing's increased capacity to model classical data with quantum states and the ability to run more complex permutations of problems can be employed in neuroscience applications such as neural signaling and synaptic integration. State-of-the-art methods are discussed such as quantum machine learning, tensor networks, Born machines, quantum kernel learning, wavelet transforms, Rydberg atom arrays, ion traps, boson sampling, graph-theoretic models, quantum optical machine learning, neuromorphic architectures, spiking neural networks, quantum teleportation, and quantum walks.Quantum Computing for the Brain is a comprehensive one-stop resource for an improved understanding of the converging research frontiers of foundational physics, information theory, and neuroscience in the context of quantum computing.
Download or read book Identification of an Operant Learning Circuit by Whole Brain Functional Imaging in Larval Zebrafish written by Jennifer Li and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: When confronted with changing environments, animals can generally adjust their behavior to optimize reward and minimize punishment. The process of modifying one's behavior based on its consequences is referred to as operant or instrumental learning. Operant learning makes specific demands on the animal. The animal must exhibit some flexibility in its behavior, switching from unsuccessful motor responses to potentially successful ones. The animal must represent the consequence of its actions. Finally, the animal must select the correct response based on its past history of reinforcement. Studies in mammalian systems have found competing and complementary circuits in the cortex and striatum that mediate different aspects of this learning process. The larval zebrafish is an ideal system to extend the study of operant learning due to its genetic and optical properties. We have developed a behavioral paradigm and imaging system that have allowed us to comprehensively image neural activity throughout the zebrafish brain during the process of operant conditioning. Our analysis of the neural network activity underlying this learning process reveals several classes of neurons whose activity correlates with learning and decision making. The distribution of these learning-related neurons is highly localized to regions of the habenula and forebrain. We describe, in particular, a lateralized habenula circuit that may encode prediction and relief prediction error.
Download or read book The World According to Zebrafish How Neural Circuits Generate Behaviour written by and published by . This book was released on 2014 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding how the brain functions is one of the most ambitious current scientific goals. This challenge will only be accomplish by a multidisciplinary approach involving genetics, molecular biology, optics, ethology, neurobiology and mathematics and using tractable model systems. The zebrafish larva is a transparent genetically tractable small vertebrate, ideal for the combination state-of-the- art imaging techniques (e.g. two-photon scanning microscopy, single-plane illumination microscopy, spatial light modulator microscopy and lightfield microscopy), bioluminiscence and optogenetics to monitor and manipulate neuronal activity from single specific neurons up to the entire brain, in an intact behaving organism. Furthermore, the zebrafish model offers large and increasing collection of mutant and transgenic lines modelling human brain diseases. With these advantages in hand, the zebrafish larva became in the recent years, a novel animal model to study neuronal circuits and behaviour, taking us closer than ever before to understand how the brain controls behaviour.
Download or read book Perceptual Decision Making in Larval Zebrafish Revealed by Whole brain Imaging written by Elena I. Dragomir and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Dissection of Complex Behavior and Whole Brain Functional Mapping in Larval Zebrafish written by Xiuye Chen and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Simultaneous whole-brain functional imaging should in principle provide a comprehensive understanding of the functional organization of the brain. However, the sheer quantity of neurons in whole-brain data makes extracting such an understanding a daunting task. An important first step is breaking up the neurons into functionally related clusters. These clusters then form manageable units for understanding and modeling the brain. Leveraging recent advances in light-sheet calcium imaging of behaving larval zebrafish, we recorded the ~100,000 neurons of the entire brain simultaneously at single-neuron resolution. We then employed a custom clustering algorithm to automatically classify neurons into functional clusters (~100 clusters per animal), characterized the sensory-motor transformations and compared multiple stimulus conditions. To facilitative in-depth exploratory analysis of whole-brain functional data, we constructed an interactive analytical software platform. This approach allowed us to both identify several known functional brain regions as well as discover previously uncharacterized activity patterns. In particular, the anterior hindbrain region shows great promise as an important site for sensory-motor transformations.
Download or read book Whole brain Functional and Structural Examination in Larval Zebrafish written by David Grant Colburn Hildebrand and published by . This book was released on 2015 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation describes new tools for enhancing larval zebrafish activity mapping endeavors and the development of a serial-section electron microscopy approach to accomplish dense structural imaging of the complete brain. Together, these developments provide a foundation for studying neuronal network computation in the context of a behaving animal.
Download or read book Handbook of Neurophotonics written by Francesco S. Pavone and published by CRC Press. This book was released on 2020-05-10 with total page 439 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook of Neurophotonics provides a dedicated overview of neurophotonics, covering the use of advanced optical technologies to record, stimulate, and control the activity of the brain, yielding new insight and advantages over conventional tools due to the adaptability and non-invasive nature of light. Including 32 colour figures, this book addresses functional studies of neurovascular signaling, metabolism, electrical excitation, and hemodynamics, as well as clinical applications for imaging and manipulating brain structure and function. The unifying theme throughout is not only to highlight the technology, but to show how these novel methods are becoming critical to breakthroughs that will lead to advances in our ability to manage and treat human diseases of the brain. Key Features: Provides the first dedicated book on state-of-the-art optical techniques for sensing and imaging across at the cellular, molecular, network, and whole brain levels. Highlights how the methods are used for measurement, control, and tracking of molecular events in live neuronal cells, both in basic research and clinical practice. Covers the entire spectrum of approaches, from optogenetics to functional methods, photostimulation, optical dissection, multiscale imaging, microscopy, and structural imaging. Includes chapters that show use of voltage-sensitive dye imaging, hemodynamic imaging, multiphoton imaging, temporal multiplexing, multiplane microscopy, optoacoustic imaging, near-infrared spectroscopy, and miniature neuroimaging devices to track cortical brain activity.
Download or read book The Mechanosensory Lateral Line written by Sheryl Coombs and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 712 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume represents the published proceedings of an international conference on the Neurobiology and Evolution of the Mechanosensory Lateral Line System held August 31 to September 4, 1987, at the Center for Interdisciplinary Research at the University of Bielefeld, West Germany. The goal of this confer ence was to bring together researchers from all over the world to share informa tion about a major aquatic sensory system, the evolution and function of which have largely remained an enigma since the 18th century. The "lateral line" or "lateralis" system has been used as an umbrella term to describe what originally (without the aid of modern anatomical techniques) looked like a series of pits, grooves, and lines on the head and trunk of fishes and some amphibians. For at least the past 30 years, however, it has been recognized that the lateralis system comprises not one, but at least two functional classes of receptors: mechanoreceptors and electroreceptors. The relative ease with which the appropriate stimulus could be defined and measured for the electroreceptive class has resulted in an explosion of information on this submodality during the past 20 years. As a result, there is little ambiguity about the overall function of the electrosensory system, now generally regarded as an independent system in its own right. A similarly clear definition for the function of the mechanosensory lateralis system has not been as forthcoming.
Download or read book Decoding Neural Circuit Structure and Function written by Arzu Çelik and published by Springer. This book was released on 2017-07-24 with total page 517 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers representative examples from fly and mouse models to illustrate the ongoing success of the synergistic, state-of-the-art strategy, focusing on the ways it enhances our understanding of sensory processing. The authors focus on sensory systems (vision, olfaction), which are particularly powerful models for probing the development, connectivity, and function of neural circuits, to answer this question: How do individual nerve cells functionally cooperate to guide behavioral responses? Two genetically tractable species, mice and flies, together significantly further our understanding of these processes. Current efforts focus on integrating knowledge gained from three interrelated fields of research: (1) understanding how the fates of different cell types are specified during development, (2) revealing the synaptic connections between identified cell types (“connectomics”) using high-resolution three-dimensional circuit anatomy, and (3) causal testing of how iden tified circuit elements contribute to visual perception and behavior.
Download or read book Imaging Manipulation and Optogenetics in Zebrafish written by Itia Amandine Favre-Bulle and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The work described here investigates the advantages and limitations of using laser light for the deep in-vivo illumination and micromanipulation of the neuronal system in zebrafish. To do so, it combines and develops novel optical methods such as optogenetics, light sheet microscopy and optical micromanipulation. It also demonstrates, for the first time, that directional and focused laser beams can successfully be used to target large objects at considerable depth in a living organism to exert purely optical force - in this case on otoliths (ear stones) - and create fictive vestibular stimuli in a stationary animal. The behavioural study and simultaneous imaging of the whole brain reveal the location of the brain cells specific to each ear stone. Elucidating these fundamental neural processes holds substantial value for basic neuroscience researchers, who still have only a vague grasp of how brain circuits mediate perception. As such, it represents highly innovative research that has already led to high-impact publications and is now being intensively pursued.
