Download or read book Western Blotting Guru written by Ayaz Najafov and published by Academic Press. This book was released on 2017-07-07 with total page 101 pages. Available in PDF, EPUB and Kindle. Book excerpt: Western Blotting Guru provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with immunoblotting setup and optimization. As a laboratory guide, it emphasizes the technical aspects of efficiently employing immunoblotting as a tool in molecular biology laboratories. The book covers the basic science underlying immunoblotting and detailed description of the method parameters, followed by good benchtop practices, tips and tricks for obtaining high-quality data and a detailed troubleshooting guide addressing a variety of problem types. Provides a benchtop reference that every molecular biologists will use to design, optimize, troubleshoot and analyze their immunoblotting experiments Contains unique good practices and tips that are indispensable for the beginner and expert alike Features special cases with applications of immunoblotting optimization Includes detailed appendices with tables, figures and key protocols Provides troubleshooting tips for various types of modifications of standard protocols Organized as a systematic, concentrated resource to save time when addressing an immunoblotting problem

Download or read book Western Blotting for the Non Expert written by Biji T. Kurien and published by Springer Nature. This book was released on 2021-07-24 with total page 467 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book fills the need for a simplified text covering western blotting protocols aimed not just at high school and college students, but the researcher with little to no experience in these techniques. It provides the principles, basic methodology, and tips and tricks to avoiding the common pitfalls of western blotting. The book also introduces simple protocols that can transform western blotting into a fun method, such as sending secret messages on membranes or using nitrocellulose membrane as a canvas for art. In addition to the techniques, this book also covers the history of western blotting, which originated from the development of the blotting of DNA. It then delves into the importance of protein blotting, brought to the fore by the fact that the procedure has been evolving constantly since its inception in 1979, and the fact that the scientific community is faced with a multitude of ways and means of transferring proteins to membranes..

Download or read book Western Blotting written by Biji T. Kurien and published by . This book was released on 2015 with total page 509 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Western Blot Simple Guide and Important Tips for a Beginner written by Che Aishah Nazariah Ismail, Mahaneem Mohamed, Victor Udo Nna and published by Penerbit USM. This book was released on 2023-10-11 with total page 91 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first time you know that you need to conduct Western blotting, you may be nervous since there are many critical steps to follow. It would be advantageous to the experienced researcher, but for the first-timer, especially postgraduate students, it can be such a hassle! This book will guide the researcher who is new to Western blotting, step by step from the scratch, in a simple and illustrative way. This book benefits the reader as it provides a simple illustrative guide starting from protein extraction to visualization of protein bands with some troubleshooting guides to improve the protein bands quality.

Download or read book Protein Blotting and Detection written by Biji T. Kurien and published by . This book was released on 2009-01-01 with total page 588 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In Protein Blotting and Detection: Methods and Protocols, expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques as well as completely original methods of protein blotting. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, step-by-step methods, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Innovative and highly practical, Protein Blotting and Detection: Methods and Protocols is an essential, hands-on guide for all investigators who hope to bring these cutting-edge procedures home to their laboratories.

Download or read book Self study Course 3031 G written by and published by . This book was released on 1990 with total page 130 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Detection of Blotted Proteins written by Biji T. Kurien and published by Humana. This book was released on 2016-10-15 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume provides methods and techniques for detection after blotting. Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Detection of Blotted Proteins: Methods and Protocols presents numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting.

Download or read book Molecular Genetics of Cancer written by John K. Cowell and published by Gulf Professional Publishing. This book was released on 2001 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first volume was published, there has been significant success in isolating genes responsible for particular cancers as well as a major improvement in our understanding of the molecular events leading to tumors. This book explores possible genetic treatments that can suppress cancer cells that have formed tumors and it presents the details of the isolation and characterization of new human cancer genes that have recently been identified. Molecular Genetics of Cancer, 2E is an essential book for anyone involved in cancer research and the search for a cure.

Download or read book Molecular Genetics of Cancer written by John Cowell and published by Garland Science. This book was released on 2003-12-16 with total page 537 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Genetics of Cancer, Second Edition provides an authoritative and up to date review of the key genes known to be critical in the development or progression of cancer. Throughout the book, scientific advances and their clinical relevance are covered in detail, particularly in the light of findings concerning the inheritance of genes predisposing to tumorigenesis. The book is therefore a valuable source of reference for clinicians and genetic counsellors as well as researchers.

Download or read book Olivia Joules and the Overactive Imagination written by Helen Fielding and published by Penguin. This book was released on 2005-04-26 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: At the close of the last millennium, Helen Fielding debuted the irrepressible (and blockbuster-bestselling) Bridget Jones. Now, Fielding gives us a sensational new heroine for a new era...Move over 007, a stunning, sexy-and decidedly female-new player has entered the world of international espionage. Her name is Olivia Joules (that’s "J.O.U.L.E.S. the unit of kinetic energy") and she's ready to take America by storm with charm, style, and her infamous Overactive Imagination. How could a girl not be drawn to the alluring, powerful Pierre Ferramo-he of the hooded eyes, impeccable taste, unimaginable wealth, exotic international homes, and dubious French accent? Could Ferramo really be a major terrorist bent on the Western world’s destruction, hiding behind a smokescreen of fine wines, yachts, and actresses slash models? Or is it all just a product of Olivia Joules’s overactive imagination? Join Olivia in her heart-stopping, hilarious, nerve-frazzling quest from hip hotel to eco-lodge to underwater cave, by light aircraft, speedboat, helicopter, and horse, in this witty, contemporary, and utterly unputdownable novel deluxe.

Download or read book Phytochemical Genomics written by Mallappa Kumara Swamy and published by Springer Nature. This book was released on 2023-01-01 with total page 691 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive reference for various plant bioactive compounds for research and pharmacological significance across the entire spectrum of phytochemical genomics. The book opens with general information on diversity, analysis and genomic basis of phytochemicals, computational approaches, databases for responsible genes, and biosynthetic pathways, and it delves very much into the details behind phytochemical diversity and diverse roles of plant metabolites. The later parts of the book also explore the direct drug discovery and omics approaches including metabolomics, transcriptomics, as well as gene editing technology experiments to further inspire readers into its unlimited potentials. Each chapter includes detailed analysis and relevant experiments for better and deeper understanding of the concepts. The book will be an invaluable aid for medicinal plant researchers and a rich source of information and advice for advanced undergraduates and graduates in the fields of medicine, nutraceuticals, cosmetics, flavor, and fragrance studies.

Download or read book Sadhguru More Than a Life written by Arundhathi Subramaniam and published by Penguin Books India. This book was released on 2010 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: ‘The thirst to be boundless is not created by you; it is just life longing for itself.’ —Sadhguru This is the extraordinary story of Sadhguru—a young agnostic who turned yogi, a wild motorcyclist who turned mystic, a sceptic who turned spiritual guide. Pulsating with his razor-sharp intelligence, bracing wit and modern-day vocabulary, the book empowers you to explore your spiritual self and could well change your life. It seeks to re-create the life journey of a man who combines rationality with mysticism, irreverence with compassion, ancient wisdom with a provocatively contemporary outlook and a deep knowledge of the self with a contagious love of life. Described as ‘a profound mystic, visionary humanitarian and prominent spiritual leader of our times', he is equally at home in a satsangh in rural Tamil Nadu as at the World Economic Forum in Davos. In his early years, Jaggi Vasudev (or Sadhguru as he is now known) was a chronic truant, a boisterous prankster, and later a lover of motorbikes and fast cars. It is evident that the same urgency, passion and vitality echo in his spiritual pursuits to this day, from his creation of the historic Dhyanalinga—the mission of three lifetimes—to his approach as a guru. In Sadhguru's view, faith and reason, spirituality and science, the sacred and the material, cannot be divided into easy binaries. He sees people as ‘spiritual beings dabbling with the material rather than the reverse’, and liberation as the fundamental longing in every form of life. Truth for him is a living experience instead of a destination, a conclusion, or a matter of metaphysical speculation. The possibility of self-realization, he strongly believes, is available to all. Drawing upon extended conversations with Sadhguru, interviews with Isha colleagues and fellow meditators, poet Arundhathi Subramaniam presents an evocative portrait of a contemporary mystic and guru—a man who seems to pack the intensity and adventure of several lifetimes into a single one.

Download or read book The Three Hostages written by John Buchan and published by Read Books Ltd. This book was released on 2015-04-24 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: The fourth of the five Richard Hannay novels by John Buchan. Here we find our hero Richard Hannay living a quiet life in the countryside with a wife and young child but his past comes back to haunt him and he once more must face up to an arch-enemy.

Download or read book Ubiquitin Proteasome System written by Aldrin V. Gomes and published by Nova Medicine & Health. This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, major advancements in our understanding of the ubiquitin-proteasome system (UPS) have occurred. This book focuses on recent trends in the UPS. The UPS is possibly the most complex of all intracellular pathways as close to 7% of all genes in the human genome make up part of the UPS. This complex system serves as an essential role in intracellular protein degradation, and because of its critical function, improper functioning of the UPS is associated with nearly all know diseases, including cancer, cardiovascular disease, and neurological diseases. The proteolytic component of the UPS is the proteasome, a multicatalytic complex found in the nucleus and cytoplasm. Another form of the proteasome, the immunoproteasome, is less abundant than the constitutive proteasome, but is important in immune response and degradation of oxidized proteins, and recent research suggests that it may be important in longevity. The articles in this book discuss recent findings which indicate that mutations in proteins involved with the UPS are associated with genetic diseases such as familial dilated cardiomyopathy, Nakajo syndrome, and spinal muscular atrophy (X-linked). Some chapters also discuss recent results which suggest that the UPS is heavily regulated by post-translational modifications such as phosphorylation, acetylation, and methylation. The UPS is also heavily regulated by ubiquitination itself. This book contains a research article using PubMed bibliometric data to present current research trends in the UPS. Articles are written so that no one tissue is emphasized to allow readers from any discipline to benefit from this information.

Download or read book Corporate Governance written by Robert A. G. Monks and published by Wiley-Blackwell. This book was released on 2003-12-19 with total page 584 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the wake of the dramatic series of corporate meltdowns: Enron; Tyco; Adelphia; WorldCom; the timely new edition of this successful text provides students and business professionals with a welcome update of the key issues facing managers, boards of directors, investors, and shareholders. In addition to its authoritative overview of the history, the myth and the reality of corporate governance, this new edition has been updated to include: analysis of the latest cases of corporate disaster; An overview of corporate governance guidelines and codes of practice in developing and emerging markets new cases: Adelphia; Arthur Andersen; Tyco Laboratories; Worldcom; Gerstner's pay packet at IBM Once again in the new edition of their textbook, Robert A. G. Monks and Nell Minow show clearly the role of corporate governance in making sure the right questions are asked and the necessary checks and balances in place to protect the long-term, sustainable value of the enterprise. A CD-ROM containing a comprehensive case study of the Enron collapse, complete with senate hearings and video footage, accompanies the text. Further lecturer resources and links are available at www.blackwellpublishing.com/monks

Download or read book Operations Management in Context written by Frank Rowbotham and published by Routledge. This book was released on 2012-05-23 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: Operations Management in Context provides students with excellent grounding in the theory and practice of operations management and its role within organizations. Structured in a clear and logical manner, it gradually leads newcomers to this subject through each topic area, highlighting key issues, and using practical case study material and examples to contextualize learning. Each chapter is structured logically and concludes with summary material to aid revision. Exercises and self-assessment questions are included to reinforce learning and maintain variety, with answers included at the end of the text.

Download or read book English Grammar for the Utterly Confused written by Laurie Rozakis and published by McGraw Hill Professional. This book was released on 2003-06-22 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: For students who want to learn the nuts and bolts of English grammar A generation ago, the United States public school system stopped formal instruction in English grammar and consequently created a legion of students and professionals notoriously weak in writing and language skills. English Grammar for the Utterly Confusedis a must-have for anyone who gets that “deer-in-the-headlights" look when asked to recognize a misplaced modifier or even find the verb in a sentence. This user-friendly, witty guide helps everyone-from students taking the GED to professionals writing business plans-learn the structures of English grammar and how to use them easily and proficiently. Demystifying grammar once and for all, this handbook will help all readers acquire the ability to speak and write competently, correctly, and confidently. Key features include: Exercises throughout to develop facility in writing skills Clear explanations of complex concepts Handy icons More than 200 solved problems and examples Test Yourself section in each chapter