Download or read book Use of CelR as a Model to Understand Glycoside Hydrolase Activity written by Nathaniel Jacob Kuch and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Efficient conversion of lignocellulosic biomass into chemically and biologically upgradeable components is a cornerstone of biofuel and bioproducts research. As such, learning more about how the thermochemical pretreatments and enzymes used for lignocellulosic biomass deconstruction interact with one another and the biomass used is a key part of advancing the field. This work begins with characterization of a family 9 glycoside hydrolase, CelR, determining the changes to kinetics, binding, and thermostability brought about by the presence or absence of carbohydrate binding domains. The structure of CelR is also described for the first time, and the potential mechanistic role of an active site adjacent calcium ion in coordinating residue motion to promote either substrate binding or product release is explored. Then, CelR is used as a model enzyme in the investigation of changes in lignocellulosic biomass from Îđ-valerolactone pretreatment through solid-state nuclear magnetic resonance (ssNMR) experiments. This work shows how the ratio of peaks corresponding to the 4-carbon of cellulose in lignocellulosic biomass strongly correlates with soluble sugar yields after hydrolysis with CelR. Spectral fitting is then used to deconvolute the 4-carbon peaks to identify changes in specific subdomains of the cellulose throughout pretreatment and enzyme hydrolysis. The IÎø subdomain is shown to form in the course of pretreatment; and be resistant to not only hydrolysis by CelR but also by the commercial cellulase cocktail CTec3. This thesis concludes with proposing a novel system for classifying and thinking about multifunctional cellulase activity, providing a framework that can be used to support and direct future research endeavors.This thesis represents advances in the understanding of family 9 glycoside hydrolases, which are attractive targets for study and enzyme engineering efforts for their ability to interact synergistically with both endo- and exo-cellulase classes. It also presents strong examples of using ssNMR to monitor chemical and physical changes to lignocellulosic biomass throughout the deconstruction process. The ratio of 4-carbon peaks that was found to correlate to hydrolysis yields could prove to be a potent tool for evaluating thermochemical pretreatments, while subdomain tracking was showcased as a powerful technique for identifying specific molecular changes and bottlenecks to enzymatic hydrolysis of lignocellulosic biomass. The tier system presented in the review of multifunctional cellulases advances dialogue in the field and expands what enzymes could be considered multifunctional and highlights under-explored research avenues that arise from the goal of fleshing out understanding of these tiers and how they integrate with one another.
Download or read book Sustainable Degradation of Lignocellulosic Biomass written by Anuj Chandel and published by BoD – Books on Demand. This book was released on 2013-05-15 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides important aspects of sustainable degradation of lignocellulosic biomass which has a pivotal role for the economic production of several value-added products and biofuels with safe environment. Different pretreatment techniques and enzymatic hydrolysis process along with the characterization of cell wall components have been discussed broadly. The following features of this book attribute its distinctiveness: This book comprehensively covers the improvement in methodologies for the biomass pretreatment, hemicellulose and cellulose breakdown into fermentable sugars, the analytical methods for biomass characterization, and bioconversion of cellulosics into biofuels. In addition, mechanistic analysis of biomass pretreatment and enzymatic hydrolysis have been discussed in details, highlighting key factors influencing these processes at industrial scale.
Download or read book Microbial Bioprospecting for Sustainable Development written by Joginder Singh and published by Springer. This book was released on 2018-09-18 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive overview of the use of microorganisms and microbial metabolites as a future sustainable basis of agricultural, environmental and industrial developments. It provides a holistic approach to the latest advances in the utilization of various microorganism bioprospecting including their wide range of applications, traditional uses, modern practices, and designing strategies to harness their potential. In addition, it highlights advanced microbial bioremediation approaches, including genetic manipulation, metagenomics analysis and bacteriophage-based sensors for the detection of food-borne pathogens. Lastly, it elaborates on the latest advances regarding the role of microbes in the sustainable development of various industrial products.
Download or read book Agrobacterium Biology written by Stanton B. Gelvin and published by Springer. This book was released on 2018-11-28 with total page 509 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume reviews various facets of Agrobacterium biology, from modern aspects of taxonomy and bacterial ecology to pathogenesis, bacterial cell biology, plant and fungal transformation, natural transgenics, and biotechnology. Agrobacterium-mediated transformation is the most extensively utilized platform for generating transgenic plants, but modern biotechnology applications derive from more than 40 years of intensive basic scientific research. Many of the biological principles established by this research have served as models for other bacteria, including human and animal pathogens. Written by leading experts and highlighting recent advances, this volume serves both as an introduction to Agrobacterium biology for students as well as a more comprehensive text for research scientists.
Download or read book Enzymes in Degradation of the Lignocellulosic Wastes written by Aparna B. Gunjal and published by Springer. This book was released on 2020-05-08 with total page 113 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers valuable insights into the principles, mechanisms of action and applications of traditional and novel enzymes involved in the degradation of wastes. Enzymes are biological catalysts that play an important role in various biochemical reactions. The generation of value-added products by means of these biological processes is also discussed. This book covers the use of in silico and computational methods in understanding the biodegradation processes, and reveals the importance of enzymes in various biochemical reactions and kinetics. The book’s target audience includes undergraduate and graduate students, faculty members at colleges and universities, research students, scientists and industry professionals.
Download or read book Metabolic Engineering written by Sang Yup Lee and published by John Wiley & Sons. This book was released on 2021-06-02 with total page 1075 pages. Available in PDF, EPUB and Kindle. Book excerpt: Learn more about foundational and advanced topics in metabolic engineering in this comprehensive resource edited by leaders in the field Metabolic Engineering: Concepts and Applications delivers a one-stop resource for readers seeking a complete description of the concepts, models, and applications of metabolic engineering. This guide offers practical insights into the metabolic engineering of major cell lines, including E. Coli, Bacillus and Yarrowia Lipolytica, and organisms, including human, animal, and plant). The distinguished editors also offer readers resources on microbiome engineering and the use of metabolic engineering in bioremediation. Written in two parts, Metabolic Engineering begins with the essential models and strategies of the field, like Flux Balance Analysis, Quantitative Flux Analysis, and Proteome Constrained Models. It also provides an overview of topics like Pathway Design, Metabolomics, and Genome Editing of Bacteria and Eukarya. The second part contains insightful descriptions of the practical applications of metabolic engineering, including specific examples that shed light on the topics within. In addition to subjects like the metabolic engineering of animals, humans, and plants, you’ll learn more about: Metabolic engineering concepts and a historical perspective on their development The different modes of analysis, including flux balance analysis and quantitative flux analysis An illuminating and complete discussion of the thermodynamics of metabolic pathways The Genome architecture of E. coli, as well as genome editing of both bacteria and eukarya An in-depth treatment of the application of metabolic engineering techniques to organisms including corynebacterial, bacillus, and pseudomonas, and more Perfect for students of biotechnology, bioengineers, and biotechnologists, Metabolic Engineering: Concepts and Applications also has a place on the bookshelves of research institutes, biotechnological institutes and industry labs, and university libraries. It's comprehensive treatment of all relevant metabolic engineering concepts, models, and applications will be of use to practicing biotechnologists and bioengineers who wish to solidify their understanding of the field.
Download or read book The Condensed Protocols from Molecular Cloning written by Joseph Sambrook and published by CSHL Press. This book was released on 2006 with total page 802 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.
Download or read book Biology of Termites a Modern Synthesis written by David Edward Bignell and published by Springer Science & Business Media. This book was released on 2010-10-20 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biology of Termites, a Modern Synthesis brings together the major advances in termite biology, phylogenetics, social evolution and biogeography. In this new volume, David Bignell, Yves Roisin and Nathan Lo have brought together leading experts on termite taxonomy, behaviour, genetics, caste differentiation, physiology, microbiology, mound architecture, biogeography and control. Very strong evolutionary and developmental themes run through the individual chapters, fed by new data streams from molecular sequencing, and for the first time it is possible to compare the social organisation of termites with that of the social Hymenoptera, focusing on caste determination, population genetics, cooperative behaviour, nest hygiene and symbioses with microorganisms. New chapters have been added on termite pheromones, termites as pests of agriculture and on destructive invasive species.
Download or read book Manual of Methods for General Bacteriology written by Philipp Gerhardt and published by . This book was released on 1981 with total page 546 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biosynthetic Technology and Environmental Challenges written by Sunita J. Varjani and published by Springer. This book was released on 2017-11-29 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive review of biosynthetic approaches to the production of industrially important chemicals and the environmental challenges involved. Its 19 chapters discuss different aspects of biosynthetic technology from the perspective of leading experts in the field. It covers various biorefinery approaches, including the use of microbes, metabolically engineered plants, biomass-based and green technology methods. Further, it examines important research in the areas of organic and hazardous waste composting, management and recovery of nutraceuticals from agro-industrial waste, biosynthesis and technological advancements of biosurfactants and waste water bioremediation. This book contributes to the scientific literature on biosynthetic technologies and the related environmental challenges for researchers and academics working in this area around the globe.
Download or read book Thermophilic Microorganisms written by Fu-li Li and published by Caister Academic Press Limited. This book was released on 2015 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thermophilic microorganisms thrive in a variety of marine and terrestrial habitats. These organisms have evolved several biochemical and molecular strategies to counteract the deleterious effects of the high temperatures in their environments. In this book, leading scientists highlight the current progress in the most topical areas of research providing a timely overview of the field. The authors discuss current technical challenges and future development trends.--
Download or read book Enzymatic Degradation of Insoluble Carbohydrates written by John N. Saddler and published by . This book was released on 1995 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents a review of enzymes used in the conversion of renewable feedstocks such as starch and cellulose. Provides examples of the use of enzymes in the resource sector, specifically addressing their use in agriculture, forest products, and pulp and paper. Explores greater use of agriculture and forestry residues and possible enzymatic modification.
Download or read book Current Developments in Solid state Fermentation written by Ashok Pandey and published by Springer Science & Business Media. This book was released on 2008-09-16 with total page 521 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the period of last two decades, there has been significant resurgence in solid-state fermentation due to the numerous benefits it offers, especially in the engineering and environmental aspects. SSF has shown much promise in the development of several bioprocesses and products. This resurgence gained further momentum during the last 5-6 years with the developments in fundamental and applied aspects. A good deal of information has been generated in published literature and patented information. Several commercial ventures have come up based on SSF in different parts of the world. The contents are organized into four parts: Part 1 deals with the General and Fundamentals aspects of SSF; Part 2 deals with the production of bulk chemicals and products such as enzymes, organic acids, spores and mushrooms in SSF; Part 3 is on the use of SSF for specialty chemicals such as gibberellic acid, antibiotics and other pharmaceutically valuable secondary metabolites, pigments, and aroma compounds; Part 4 deals with the use of SSF miscellaneous application such as SSF for food and feed applications, agro-industrial residues as substrates in SSF and the production of silage and vermicompost.
Download or read book Enzymatic Conversion of Biomass for Fuels Production written by Michael E. Himmel and published by . This book was released on 1994 with total page 522 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discusses the use of enzymatic and microbial biocatalysis for transformation of biomass to liquid or gaseous fuels. Explores metabolic pathway engineering. Discusses characterization of new hydrolytic enzymes. Presents new microorganisms and fermentation techniques. Focuses on lignocellulosic biomass conversion technology.
Download or read book Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals written by Charles E. Wyman and published by John Wiley & Sons. This book was released on 2013-03-27 with total page 597 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant biomass is attracting increasing attention as a sustainable resource for large-scale production of renewable fuels and chemicals. However, in order to successfully compete with petroleum, it is vital that biomass conversion processes are designed to minimize costs and maximize yields. Advances in pretreatment technology are critical in order to develop high-yielding, cost-competitive routes to renewable fuels and chemicals. Aqueous Pretreatment of Plant Biomass for Biological and Chemical Conversion to Fuels and Chemicals presents a comprehensive overview of the currently available aqueous pretreatment technologies for cellulosic biomass, highlighting the fundamental chemistry and biology of each method, key attributes and limitations, and opportunities for future advances. Topics covered include: • The importance of biomass conversion to fuels • The role of pretreatment in biological and chemical conversion of biomass • Composition and structure of biomass, and recalcitrance to conversion • Fundamentals of biomass pretreatment at low, neutral and high pH • Ionic liquid and organosolv pretreatments to fractionate biomass • Comparative data for application of leading pretreatments and effect of enzyme formulations • Physical and chemical features of pretreated biomass • Economics of pretreatment for biological processing • Methods of analysis and enzymatic conversion of biomass streams • Experimental pretreatment systems from multiwell plates to pilot plant operations This comprehensive reference book provides an authoritative source of information on the pretreatment of cellulosic biomass to aid those experienced in the field to access the most current information on the topic. It will also be invaluable to those entering the growing field of biomass conversion.
Download or read book Biofuels written by Jonathan R. Mielenz and published by Humana Press. This book was released on 2012-03-07 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: This editor believes the public finally understands that bioenergy should be a critical new 39 component of our world’s future energy supply. While this new enlightenment might have 40 come from bioenergy’s environmentally friendly attributes and its renewable and sustain- 41 able nature, the reality is that the primary driver has been both the high cost of energy, 42 led recently by petroleum products, and the unique climatic changes such as local weather 43 events and reports of melting of ancient glaciers. I believe that much of the public under- 44 stands the primary cause of these changes is the growing demand for fossil fuels for heat- 45 ing and transportation needs driven by both population increases and dramatic economic 46 development around the globe. This increased demand, as well as global politics, has 47 impacted the costs for these energy products. At the same time, increased use of fossil fuels 48 has been liberating unprecedented levels of CO and other greenhouse gasses (GHGs) 49 2 that must be impacting the global climate. As a result, the world is looking for alternative 50 solutions. Renewable energy is one of the leading options as long as it is pursued in a sus- 51 tainable fashion. Of course, renewable energy has numerous sources, including wind, solar, 52 geothermal, ocean currents/tides, and bio-based energy. The latter category of renewable 53 energy is centered primarily on use of green plant matter, i. e.
Download or read book Direct Microbial Conversion of Biomass to Advanced Biofuels written by Michael E Himmel and published by Elsevier. This book was released on 2015-05-19 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: 'Direct Microbial Conversion of Biomass to Advanced Biofuels' is a stylized text that is rich in both the basic and applied sciences. It provides a higher level summary of the most important aspects of the topic, addressing critical problems solved by deep science. Expert users will find new, critical methods that can be applied to their work, detailed experimental plans, important outcomes given for illustrative problems, and conclusions drawn for specific studies that address broad based issues. A broad range of readers will find this to be a comprehensive, informational text on the subject matter, including experimentalists and even CEOs deciding on new business directions. Describes an important new field in biotechnology, the consolidated conversion of lignocellulosic feedstocks to advanced fuels Up-to-date views of promising technologies used in the production of advanced biofuels Presents the newest ideas, well-designed experiments, and outcomes Provides outstanding illustrations from NREL and contributing researchers Contains contributions from leaders in the field that provide numerous examples and insights into the most important aspects of the topic
