Download or read book Aqueous Two Phase Systems written by Rajni Hatti-Kaul and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: A mixture of two polymers, or one polymer and a salt, in an aqueous medium separates into two phases: this phenomenon is useful in biotechn- ogy for product separations. Separation of biological molecules and particles in these aqueous two-phase systems (ATPS) was initiated over 40 years ago by P.-Å. Albertsson, and later proved to be of immense utility in biochemical and cell biological research. A boost in the application of ATPS was seen when problems of separations in biotechnology processes were encountered. Its simplicity, biocompatibility, and amenability to easy scaleup operations make the use of ATPS very attractive for large-scale bioseparations. Despite the advantages ATPS enjoys over other separation techniques, the application of two-phase systems has for a long time been confined to selected labora- ries. Recent years have, however, shown a trend in which increasing numbers of researchers employ two-phase partitioning techniques in both basic and applied research.
Download or read book Partitioning In Aqueous Two Phase System written by Harry Walter and published by Elsevier. This book was released on 2012-12-02 with total page 731 pages. Available in PDF, EPUB and Kindle. Book excerpt: Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology is a collection of papers that discusses the applications of aqueous two-phase systems to problems of separation and extraction of macromolecules, organelles, and cells. Papers focus on the theoretical basis and the practical details of the procedures used. Some of the papers describe in one or a few steps how two components can be separated by the investigator manipulating their partitions so that one component is in one phase and the other component is in the other phase or at the interface. Investigators can also avail of developed batch extractions for plant organelles, cell membranes, nucleic acids, and proteins. The book cites as an example the partitioning of right-side-out and inside-out vesicles (obtained from fragments of thylakoid membranes) to the top and bottom phases, respectively, of a Dx-PEG system. Other papers describe the use of the countercurrent distribution when single extraction steps are not sufficient to produce a separation in materials that do not differ greatly in their partitioning behavior. The collection can prove valuable for bio-chemists, cellular biologists, micro-biologists, and developmental biologists.
Download or read book Aqueous Two Phase Systems written by Vangelis Anatolijs Xanthopoulos and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aqueous Two-Phase Systems (ATPS) have been used since their discovery for the isolation of a large number of different biological materials, such as animal and plant cells, microorganisms, viruses, cellular organelles such as chloroplasts, mitochondria, membrane vesicles, and also in the purification of proteins and nucleic acids. In Aqueous Two-Phase Systems: Properties, Functions and Advantages, the authors begin by discussing the benefits of these systems.The following chapter presents the main forms of the purification of lipases from aqueous biphasic systems, addressing and discussing the fundamentals for the formation, use and optimization of these systems applied in the biotechnological context. The authors address the basis of aqueous-biphasic systems, aqueous two-phase systems for enzymatic purification, purification of lipases and combined uses of aqueous two-phase systems.The most important experimental parameters that affect the efficiency of metal ions extraction in aqueous PEG-based two-phase systems are examined to provide a starting point in the design of a suitable system for the extraction of metal ions. Compared to the conventional solvent extraction, which uses toxic, flammable and volatile organic solvents and can be quite expensive, the extraction in aqueous PEG-based two-phase systems is considered a more environmental friendly and economically viable method.Also in this compilation, an overview about application of ATPSs in microstructured devices is provided. Microstructured devices offer potential benefits due to well-defined high specific interfacial areas available for heat and mass transfer. These areas increase transfer rate and enhance yield, selectivity and process control. The application of ATPSs in Micro Total Analysis Systems is described as well.
Download or read book Aqueous Two Phase Systems for Bioprocess Development for the Recovery of Biological Products written by Marco Rito-Palomares and published by Springer. This book was released on 2017-07-18 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive and unique text presents a full overview of downstream processing useful for those new to the concept as well as professionals with experience in the area. The history and theoretical principles of Aqueous Two-Phase Systems (ATPS) are covered in depth. Information on ATPS characterization and application is included, and ATPS equilibria and system parameters that have significant effect on partition behavior are studied. Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products addresses specific applications of ATPS for the recovery and partial purification of high molecular weight compounds such as proteins, nucleic acids and polysaccharides, particulate bioproducts such as cells and organelles and low molecular weight compounds. Non-conventional strategies involving ATPS such as affinity systems, continuous liquid-liquid fractionation stages and the recovery from plant extracts are presented. Economic analysis of the application of ATPS in comparison to other fractionation techniques, particularly liquid chromatography, is considered, as are opportunity and current trends in the ATPS research area. Each chapter utilizes the contributors' experimental expertise in traditional and non-conventional ATPS strategies, as well as analysis of areas of opportunity and perspectives on the development and future applications of ATPS in both the lab and larger scale operations. The result is a thorough and singular overview of ATPS which has not been matched by any other text on the market.
Download or read book Two Phase Flow in Complex Systems written by Salomon Levy and published by John Wiley & Sons. This book was released on 1999-08-02 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mehrphasige Strömungen spielen in etlichen Industriezweigen, besonders der Luft- und Raumfahrt und der Energieerzeugung, eine zentrale Rolle. Derart komplexe Strömungsvorgänge sind extrem schwer vorauszuberechnen, zu analysieren und zu testen. Wertvolle Hilfestellungen, die für reale technische Situationen gedacht sind, gibt dieser Band, der auch Quelltexte einschlägiger Computerprogramme enthält. (07/99)
Download or read book Separation of Isotopes of Biogenic Elements in Two phase Systems written by Boris Mikhailovich Andreev and published by Elsevier. This book was released on 2006-12-01 with total page 317 pages. Available in PDF, EPUB and Kindle. Book excerpt: Separation of Isotopes of Biogenic Elements provides a detailed overview of this area of research covering all aspects from the value of isotope effects to their practical use (equilibrium single-stage isotope effect - kinetics and mass transfer – multiplication of the single-stage isotope separation factor - technological peculiarity of processes) with the purpose of extraction from the natural mixture of the enriched and highly concentrated isotopes. In contrast to traditional books on the theory of isotope separation, the theoretical part of the book describes separation in two-phase processes in counter-flow columns. The experimental part of the book presents systematic analysis of specialists in the field of isotope separation in counter-flow columns. This book will be of interest to scientists, engineers and technical workers engaged in isotope separation processes and isotope application in nuclear physics, medicine, agro-chemistry, biology and other areas. This book may also be used in teaching theory and practical aspects in courses on physical chemistry and Isotope separation of light elements by physicochemical methods. * summarises current state of isotope research, especially biogenic elements* covering all aspects from the value of isotope effects to their practical use* of interest to scientists, engineers and technical workers engaged in isotope separation processes and isotope application
Download or read book Single phase Two phase and Supercritical Natural Circulation Systems written by Pallippattu Krishnan Vijayan and published by Woodhead Publishing. This book was released on 2019-06-19 with total page 650 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single-Phase, Two-Phase and Supercritical Natural Circulation Systems provides readers with a deep understanding of natural circulation systems. This book equips the reader with an understanding on how to detect unstable loops to ensure plant safety and reliability, calculate heat transport capabilities, and design effective natural circulation loops, stability maps and parallel channel systems. Each chapter begins with an introduction to the circulation system before discussing each element in detail and analyzing its effect on the performance of the system. The book also presents thermosyphon heat transport devices in nuclear and other industrial plants, a common information need for students and researchers alike. This book is invaluable for engineers, designers, operators and consultants in nuclear, mechanical, electrical and chemical disciplines. Presents single-phase, two-phase and supercritical natural circulation systems together in one resource to fill an existing knowledge gap Guides the reader through relevant processes, such as designing, analyzing and generating stability maps and natural circulation loops, calculating heat transport capabilities, and maintaining natural circulation system operations Includes global case studies and examples to increase understanding, along with important IAEA standards and procedures
Download or read book Thermo fluid Dynamics of Two Phase Flow written by Mamoru Ishii and published by Springer Science & Business Media. This book was released on 2006-09-28 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has been written for graduate students, scientists and engineers who need in-depth theoretical foundations to solve two-phase problems in various technological systems. Based on extensive research experiences focused on the fundamental physics of two-phase flow, the authors present the detailed theoretical foundation of multi-phase flow thermo-fluid dynamics as they apply to a variety of scenarios, including nuclear reactor transient and accident analysis, energy systems, power generation systems and even space propulsion.
Download or read book Aqueous Two Phase Systems written by Rajni Hatti-Kaul and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins - including a glimpse of large-scale handling of two-phase separations. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.
Download or read book Aqueous Two Phase Partitioning written by Boris Y. Zaslavsky and published by CRC Press. This book was released on 1994-11-15 with total page 720 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers the fundamental principles of solute partitioning in aqueous two-phase systems, explains their important practical features, and furnishes methods of characterization. The information provided by the partition behaviour of a solute in an aqueous two-phase system is examined.
Download or read book Two Phase Flow Boiling and Condensation written by S. Mostafa Ghiaasiaan and published by Cambridge University Press. This book was released on 2014-08-07 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text is an introduction to gas-liquid two-phase flow, boiling and condensation for graduate students, professionals, and researchers in mechanical, nuclear, and chemical engineering. The book provides a balanced coverage of two-phase flow and phase change fundamentals, well-established art and science dealing with conventional systems, and the rapidly developing areas of microchannel flow and heat transfer. It is based on the author's more than 15 years of teaching experience. Instructors teaching multiphase flow have had to rely on a multitude of books and reference materials. This book remedies that problem by covering all the topics that are essential for a graduate first course. Among the important areas that are discussed in the book, and are not adequately covered by virtually all the available textbooks, are: two-phase flow model conservation equations and their numerical solution; condensation with and without noncondensables; and two-phase flow, boiling, and condensation in mini and microchannels.
Download or read book Computational Methods For Two phase Flow And Particle Transport With Cd rom written by Wen Ho Lee and published by World Scientific Publishing Company. This book was released on 2013-03-22 with total page 471 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes mathematical formulations and computational methods for solving two-phase flow problems with a computer code that calculates thermal hydraulic problems related to light water and fast breeder reactors. The physical model also handles the particle and gas flow problems that arise from coal gasification and fluidized beds. The second part of this book deals with the computational methods for particle transport.
Download or read book Two Phase Heat Transfer written by Mirza Mohammed Shah and published by John Wiley & Sons. This book was released on 2021-03-04 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: A guide to two-phase heat transfer theory, practice, and applications Designed primarily as a practical resource for design and development engineers, Two-Phase Heat Transfer contains the theories and methods of two-phase heat transfer that are solution oriented. Written in a clear and concise manner, the book includes information on physical phenomena, experimental data, theoretical solutions, and empirical correlations. A very wide range of real-world applications and formulas/correlations for them are presented. The two-phase heat transfer systems covered in the book include boiling, condensation, gas-liquid mixtures, and gas-solid mixtures. The authora noted expert in this fieldalso reviews the numerous applications of two-phase heat transfer such as heat exchangers in refrigeration and air conditioning, conventional and nuclear power generation, solar power plants, aeronautics, chemical processes, petroleum industry, and more. Special attention is given to heat exchangers using mini-channels which are being increasingly used in a variety of applications. This important book: Offers a practical guide to two-phase heat transfer Includes clear guidance for design professionals by identifying the best available predictive techniques Reviews the extensive literature on heat transfer in two-phase systems Presents information to aid in the design and analysis of heat exchangers. Written for students and research, design, and development engineers, Two-Phase Heat Transfer is a comprehensive volume that covers the theory, methods, and applications of two-phase heat transfer.
Download or read book Two Phase Flow Heat Exchangers written by Sadik Kakaç and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 1090 pages. Available in PDF, EPUB and Kindle. Book excerpt: Two-phase flow heat exchangers are vital components of systems for power generation, chemical processing, and thermal environment control. The art and science of the design of such heat exchangers have advanced considerably in recent years. This is due to better understanding of the fundamentals of two-phase flow and heat transfer in simple geometries, greater appreciation of these processes in complex goemetries, and enhanced predictive capability through use of complex computer codes. The subject is clearly of great fundamental and practical importance. The NATO ASIan Thermal-Hydraulic Fundamentals and Design of Two-Phase Flow Heat Exchangers was held in Povoa de Varzim (near Porto), Portugal, July 6-17, 1987. participating in the organization of" the ASI were the Department of Mechanical Engineering and the Clean Energy Research Institute, University of Miami; Universidade do Porto; and the Department of Mechanical Engineering, Aeronautical Eng ineer ing, and Mechanics, Rensselaer Polytechnic Institute. The ASI was arranged primarily as a high-level teaching activity by experts representing both academic and industrial viewpoints. The program included the presentation of invited lectures, a limited number of related technical papers and discussion sessions.
Download or read book Two Phase Flow written by Cl Kleinstreuer and published by Routledge. This book was released on 2017-11-01 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: This graduate text provides a unified treatment of the fundamental principles of two-phase flow and shows how to apply the principles to a variety of homogeneous mixture as well as separated liquid-liquid, gas-solid, liquid-solid, and gas-liquid flow problems, which may be steady or transient, laminar or turbulent.Each chapter contains several sample problems, which illustrate the outlined theory and provide approaches to find simplified analytic descriptions of complex two-phase flow phenomena.This well-balanced introductory text will be suitable for advanced seniors and graduate students in mechanical, chemical, biomedical, nuclear, environmental and aerospace engineering, as well as in applied mathematics and the physical sciences. It will be a valuable reference for practicing engineers and scientists. A solutions manual is available to qualified instructors.
Download or read book Ionic Liquid Based Aqueous Biphasic Systems written by Mara G. Freire and published by Springer. This book was released on 2016-10-05 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers comprehensive information on the fundamentals and applications of ionic-liquid-based aqueous biphasic systems, which have predominantly (and successfully) been employed as alternative platforms for the extraction, separation and purification of diverse high-value products. The book consists of an initial introduction providing a brief overview, from fundamentals to applications, followed by nine chapters addressing the respective phase diagrams (interpretation and characterization) and remarkable examples of their applications. It also includes two final chapters focusing on recent developments in the search for more environmentally-benign and biocompatible ionic-liquid-based aqueous biphasic systems, and on the progress made to date concerning the recovery, recycling and reuse of the phase-forming components, the goal being the development of cost-effective and sustainable processes. The book offers an interesting and useful guide for a broad readership in the fields of green chemistry, biotechnology, chemical engineering, and biochemistry, among others. Mara G. Freire is a Coordinator Researcher at CICECO - Aveiro Institute of Materials, Chemistry Department, University of Aveiro, Portugal.
Download or read book Two Phase Flow in Refrigeration Systems written by Junjie Gu and published by Springer Science & Business Media. This book was released on 2013-08-17 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt: Two-Phase Flow in Refrigeration Systems presents recent developments from the authors' extensive research programs on two-phase flow in refrigeration systems. This book covers advanced mass and heat transfer and vapor compression refrigeration systems and shows how the performance of an automotive air-conditioning system is affected through results obtained experimentally and theoretically, specifically with consideration of two-phase flow and oil concentration. The book is ideal for university postgraduate students as a textbook, researchers and professors as an academic reference book, and by engineers and designers as handbook.
