Download or read book Peptide Lipid Interactions written by Sidney A. Simon and published by Academic Press. This book was released on 2002-11-13 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains a comprehensive overview of peptide-lipid interactions by leading researchers. The first part covers theoretical concepts, experimental considerations, and thermodynamics. The second part presents new results obtained through site-directed EPR, electron microscopy, NMR, isothermal calorimetry, and fluorescence quenching. The final part covers problems of biological interest, including signal transduction, membrane transport, fusion, and adhesion. Key Features * world-renowned experts * state-of-the-art experimental methods * monolayers, bilayers, biological membranes * theoretical aspects and computer simulations * rafts * synaptic transmission * membrane fusion * signal transduction

Download or read book Transmembrane Peptide lipid Interactions written by Michael A. Amon and published by . This book was released on 2007 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Membrane Induced Structure in Transmembrane Signaling Proteins and Peptides written by and published by . This book was released on 2012 with total page 59 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Membrane Protein Lipid Interactions Physics and Chemistry in the Bilayer written by Jordi H. Borrell and published by Springer. This book was released on 2016-03-15 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has been conceives as a brief introduction to biomembranes physical chemistry for undergraduate students of sciences, and it is particularly dedicated to the lipid-protein membrane interactions. A general introduction is presented in Chapters 1 and 2. The following Chapters, 3 and 4, describe the most accepted theories on lipid-membrane protein interactions as well as the new experimental approaches, in particular, these arose from nano sciences as atomic for microscopy and single molecule force spectroscopy. The book emphasizes the relevance of physical parameters as the lateral surface pressure and the lipid curvature as actors for understanding the physicochemical properties of the biomembranes.

Download or read book Protein Lipid Interactions written by C.Reyes Mateo and published by Springer Science & Business Media. This book was released on 2008-09-30 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological membranes have long been identified as key elements in a wide variety of cellular processes including cell defense communication, photosynthesis, signal transduction, and motility; thus they emerge as primary targets in both basic and applied research. This book brings together in a single volume the most recent views of experts in the area of protein–lipid interactions, providing an overview of the advances that have been achieved in the field in recent years, from very basic aspects to specialized technological applications. Topics include the application of X-ray and neutron diffraction, infrared and fluorescence spectroscopy, and high-resolution NMR to the understanding of the specific interactions between lipids and proteins within biological membranes, their structural relationships, and the implications for the biological functions that they mediate. Also covered in this volume are the insertion of proteins and peptides into the membrane and the concomitant formation of definite lipid domains within the membrane.

Download or read book Protein Lipid Interactions written by Lukas K. Tamm and published by John Wiley & Sons. This book was released on 2006-05-12 with total page 470 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 17 contributions by leading research groups, this first comprehensive handbook in the field covers the interactions between proteins and lipids that make the fabric of biological membranes from every angle. It examines the relevant hermodynamic and structural issues from a basic science perspective, and goes on to discuss biochemical and cell biological processes. The book covers physical principles as well as mechanisms of membrane fusion and fission. Additionally, chapters on bilayer structure and protein-lipid interactions as well as on how proteins shape lipids and vice versa, membrane penetration by toxins, protein sorting, and allosteric regulation of signal transduction across membranes make this a valuable information source for researchers in academia and industry.

Download or read book Free Energy Calculations written by Christophe Chipot and published by Springer Science & Business Media. This book was released on 2007-01-08 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: Free energy constitutes the most important thermodynamic quantity to understand how chemical species recognize each other, associate or react. Examples of problems in which knowledge of the underlying free energy behaviour is required, include conformational equilibria and molecular association, partitioning between immiscible liquids, receptor-drug interaction, protein-protein and protein-DNA association, and protein stability. This volume sets out to present a coherent and comprehensive account of the concepts that underlie different approaches devised for the determination of free energies. The reader will gain the necessary insight into the theoretical and computational foundations of the subject and will be presented with relevant applications from molecular-level modelling and simulations of chemical and biological systems. Both formally accurate and approximate methods are covered using both classical and quantum mechanical descriptions. A central theme of the book is that the wide variety of free energy calculation techniques available today can be understood as different implementations of a few basic principles. The book is aimed at a broad readership of graduate students and researchers having a background in chemistry, physics, engineering and physical biology.

Download or read book Protein Lipid Interactions written by Anthony Watts and published by Elsevier. This book was released on 1993-10-08 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein-lipid interactions as a field of study is now a mature area, and this volume of NewComprehensive Biochemistry has been published with two objectives in mind. Firstly, to look to thefuture, and try to envisage how the subject may develop in the near to medium future. Secondly, to presentcontrasting or complementary views on the same system. For example, the acetylcholine receptor is discussedfrom a predominantly structural aspect by Barrantes, and from the kinetic standpoint by Rankin, et al. The volume not only gives an update on specific aspects of the field, but also shows the way in whichthe phenomenon of protein-lipid interactions is now seemingly infiltrating many areas of biomembraneresearch, from recombinant DNA studies, protein insertion and assembly and reconstitution considerationsto structural studies of membrane proteins.

Download or read book Membrane active Peptides written by Miguel A. R. B. Castanho and published by Internat'l University Line. This book was released on 2010 with total page 675 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Interactions of Transmembrane Peptides and Proteins with Lipid Membranes Studied by Mass Spectrometry written by Jeroen Adrianus Antonius Demmers and published by . This book was released on 2002 with total page 137 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Lipid Protein Interactions written by Jörg H. Kleinschmidt and published by Humana Press. This book was released on 2013-02-14 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological membranes are the essential structuring elements of all living cells. Many enzymatic reactions take place at the membrane-water interface. To gain detailed insight into membrane properties, it is therefore of great importance to understand the complex nature of the interactions of membrane proteins with lipids. Lipid-Protein Interactions: Methods and Protocols provides a selection of protocols to examine protein-lipid interactions, membrane and membrane protein structure, how membrane proteins affect lipids and how they are in turn affected by the lipid bilayer and lipid properties. The methods described here are all actively used, complementary, and necessary to obtain comprehensive information about membrane structure and function. They include label-free approaches, imaging techniques and spectroscopic methodologies. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Lipid-Protein Interactions: Methods and Protocols seeks to serve both professional and novices with its wide range of the methods frequently used in this area of research.

Download or read book Transmembrane Model Peptides A Powerful Tool to Investigate Protein Lipid Interactions written by A. Holt and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Progress in Protein lipid Interactions written by A. Watts and published by Elsevier Science & Technology. This book was released on 1985 with total page 316 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Peptide and Protein Interaction with Membrane Systems written by Sara Bobone and published by Springer. This book was released on 2014-05-31 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt: In her thesis, Sara Bobone outlines spectroscopic studies of antimicrobial peptides (AMPs) which are promising lead compounds for drugs used to fight multidrug resistant bacteria. Bobone shows that AMPs interact with liposomes and she clarifies the structure of pores formed by one of these molecules. These results help us to understand how AMPs are selective for bacterial membranes and how their activity can be finely tuned by modifying their sequence. Findings which solve several conundrums debated in the literature for years. In addition, Bobone uses liposomes as nanotemplates for the photopolymerization of hydrogels - exploiting the self- assembly properties of phospholipids. Bobone was able to trap an enzyme using nanometeric particles, while still allowing its activity by the diffusion of substrates and products through the network of the polymer. The innovative nano devices described in this thesis could solve many of the hurdles still hampering the therapeutic application of protein-based drugs.

Download or read book Lipid Domains written by and published by Academic Press. This book was released on 2015-06-08 with total page 393 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current Topics in Membranes is targeted toward scientists and researchers in biochemistry and molecular and cellular biology, providing the necessary membrane research to assist them in discovering the current state of a particular field and in learning where that field is heading. This volume offers an up to date presentation of current knowledge in the field of Lipid Domains. Written by leading experts Contains original material, both textual and illustrative, that should become a very relevant reference material The material is presented in a very comprehensive manner Both researchers in the field and general readers should find relevant and up-to-date information

Download or read book Biophysical Interactions of Peptides and Their Analogues with Lipid Membranes written by Anja Stulz and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Many drugs, displaying a wide range of structures and diverse applications, can cross or bind to lipid membranes. Quantitative understanding of membrane interactions is thus important for several therapeutic approaches. First, membrane permeabilization represents the dominating mode of action of antimicrobial peptides (AMPs) and their synthetic mimics (SMAMPs). In terms of clinical applications, selectivity for bacterial over mammalian membranes is as important as good activity. Second, membrane interactions might influence loading, retaining, and releasing drugs from lipid-based drug delivery systems in a time controlled and targeted manner. Understanding the binding behaviour of the peptide drug exenatide to lipid membranes is not only important for characterization of its release from vesicular phospholipid gels, but might also help to understand other complex peptide-lipid interactions. The main aim of this thesis was to derive a mechanistic understanding of interactions of peptides and their analogues with model lipid membranes with a focus on the lipid composition of a membrane. Membrane permeabilization induced by AMPs and SMAMPs was quantified by a lifetime-based leakage assay using calcein-loaded vesicles. Different leakage behaviours were identified by considering active concentrations, strengths of individual leakage events, L1, and cumulative kinetics. Further experiments using isothermal titration calorimetry (ITC), monolayer adsorption measurements, and differential scanning calorimetry (DSC) helped to characterize the initial binding of AMPs and SMAMPs to lipid membranes and their propensity to induce electrostatic lipid clustering. Leakage experiments showed that the leakage behaviour changes with both, the structure of the AMP or SMAMP and the lipid composition of the membrane. The activity seems to increase if a membrane-active agent favours a permeabilization mechanism to which the particular lipid composition is especially susceptible. A closer look at kinetic profiles allowed distinguishing leakage induced by asymmetric stress from leakage events that occur stochastically. Very hydrophobic and unselective compounds seem to act mainly by hydrophobically driven asymmetry stress, especially when acting on zwitterionic phosphatidylcholine (PC) membranes. This mechanism brings about poor selectivity because all lipid membranes (bacterial and mammalian) contain a hydrophobic core. Stochastic leakage events, on the other hand, probably depend more on lipid compositions. Negatively charged lipids like phosphatidylglycerol (PG) or cardiolipin (CL) triggered the initial electrostatic attraction of polycationic AMPs or SMAMPs to bacterial membranes. High amounts of phosphatidylethanolamine (PE) seem to counteract the unselective asymmetry stress mechanism. Finally, especially strong leakage events were induced in vesicles containing CL. In this way, compounds that induce only rare leakage events might still be effective. In the second part of the thesis, an ITC fit model was introduced to study complex peptide-lipid interactions based on primary binding of peptide to the lipid layer and secondary binding to pre-bound peptide. Exenatide served as an exemplary peptide that interacts electrostatically with mixed POPC/POPG liposomes and self-associates at Kd = 46 μM. A global fit of various ITC curves revealed that exenatide binds primarily to a binding site at the outer membrane leaflet composed of 2-3 negatively charged POPG and some POPC molecules. Primary binding showed high affinity with a Kd1 of 0.2-0.6 μM, while secondary binding with a Kd2 of 10-46 μM was weaker. ITC was able to quantify primary and secondary binding separately, based on different binding enthalpies. Unlike ITC, other methods such as tryptophan fluorescence and microscale thermophoresis (MST) probably represent only a summary or average of both effects. Many similar ITC data can be found in literature that possibly involve primary and secondary binding effects. Those data could benefit from a fit model as presented in this thesis