Download or read book Towards the Synthesis of Isotopically Labelled Amino Acids written by Rachel Mary Campbell and published by . This book was released on 2009 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Studies Towards the Synthesis of Isotopically Labelled Amino Acids written by Damiano Banfi and published by . This book was released on 1998 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Towards the Synthesis of Isotopically Labelled alpha amino Acids written by and published by . This book was released on 2005 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Synthesis of Several Isotopically Labelled Amino Acids written by Alexander Theodore Shulgin and published by . This book was released on 1955 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isotope labeling in Biomolecular NMR written by Hanudatta S. Atreya and published by Springer Science & Business Media. This book was released on 2012-10-18 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt: NMR spectroscopy has undergone a revolution in recent years with the advent of several new methods overcoming the problems of sensitivity and resolution. Recent developments in biotechnology have made it easier and economical to introduce 13C, 15N and 2H into proteins and nucleic acids. At the same time, there has been an explosion in the number of NMR experiments that utilize such isotope labeled samples. Thus, a combination of isotopic labeling and multidimensional, multinuclear NMR has opened up new avenues for structural studies of proteins, nucleic acids and their complexes. This book will focus on recent developments in isotope labeling methods for structural studies of small molecules, peptides, proteins and nucleic acids. The aim of the book is to serve as a compendium of isotope labeling for the biomolecular NMR community providing comprehensive coverage of the existing methods and latest developments along with protocols and practical hints on the various experimental aspects. The book will cover a wide range of topics in isotope labeling under one title including emerging areas of metabolonomics and solid state NMR.
Download or read book The Organic Chemistry of Isotopic Labelling written by James R Hanson and published by Royal Society of Chemistry. This book was released on 2019-04-05 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: The chemical synthesis of isotopically labelled compounds is a pre-requisite for many chemical, biochemical and medicinal investigations. The constraints imposed by the requirements for regiospecific labelling and, in some instances, the time-scale of the synthesis often lead to quite different synthetic strategies to those that are used for the unlabelled material. Whilst there are many specialist papers, reviews and long books devoted to particular isotopes, there is no currently available short introductory book devoted to the organic chemistry of isotopic labelling. The aim of this book is to introduce research workers to a variety of methods that have been used to achieve these synthetic labelling objectives before exploring a particular method in detail. It covers a number of different isotopes and the methods that have been used to introduce them into organic compounds. Labelling methods also provide useful undergraduate teaching examples of modern synthetic reactions and their stereochemical consequences using relatively simple substrates. The book will therefore have a wider appeal than just those involved in using isotopes in research such as environmental and pharmaceutical chemists as well as organic chemists.
Download or read book Synthesis and Application of Stable isotope Labelled L alpha amino Acids written by Johannes Jacobus Cappon and published by . This book was released on 1993 with total page 127 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Enantioselective Synthesis of Isotopically Labelled Amino Acids and Nitrogen Heterocycles written by and published by . This book was released on 2007 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Isotope Dilution Mass Spectrometry written by Jose Alonso and published by Royal Society of Chemistry. This book was released on 2019-03-22 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isotope Dilution Mass Spectrometry (IDMS) has become an essential tool in research laboratories and is increasingly used in routine analysis labs (including environmental, food safety and clinical applications). This is the first textbook to present a comprehensive and instructive view of the theory and applications of this growing technique. The main objective of this book is to cover the theory and applications of Isotope Dilution in Analytical Chemistry. The scope is comprehensive to include elemental analysis, speciation analysis, organic analysis and biochemical and clinical analysis together with applications in metabolism studies and traceability of goods. Until now there have been no books published with the same general scope (only book chapters on particular applications). This is a textbook focused at post-graduate level covering the basic knowledge required for doctoral studies in this field. Isotope Dilution Mass Spectrometry will also outline practical applications of interest for routine testing laboratories where isotope dilution procedures are implemented or can be implemented in the future. This unique book covers all the theoretical and practical aspects of Isotope Dilution Mass Spectrometry (IDMS). Due to the increasing application of IDMS in many research laboratories and the increasing implementation of IDMS methodologies in routine testing laboratories, scientists in industry and working in or affiliated to this area will this an invaluable source of information. Concerning the theoretical aspects, the authors present a uniform theoretical background which grows from previous developments in Organic, Speciation and Elemental analysis both in their own laboratory and in other laboratories around the world. This general approach will be simpler and will also include new emerging fields such as quantitative proteomics and metabolism studies.
Download or read book Synthesis and Applications of Isotopically Labelled Compounds 1994 written by J. Allen and published by Wiley. This book was released on 1995-08-15 with total page 990 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work provides a forum for the exchange of information between leading scientists and manufacturers involved in the synthesis and applications of compounds labelled with radioactive and stable isotopes. Contains a general overview of the latest work in the field and its future significance.
Download or read book The Preparation of Optically Active Stable Isotope Labeled Alpha amino Acids and Their Utility in the Synthesis of Other Molecules written by Eli A. Stoddard and published by . This book was released on 2007 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Quantitative Proteomics by Mass Spectrometry written by Salvatore Sechi and published by Methods in Molecular Biology. This book was released on 2018-04-15 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume describes prominent methodologies developed by laboratories that have been leading the field of quantitative proteomics by mass spectrometry. The procedures for performing the experiments are described in an easy-to-understand manner with many technical details that usually are not reported in typical research articles. This second edition of Quantitative Proteomics by Mass Spectrometry provides a broad perspective of the methodologies used for quantifying proteins and post-translational modifications in different types of biomedical specimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Quantitative Proteomics by Mass Spectrometry, Second Edition is a valuable resource to help researchers understand and learn about the latest tools used in the study of quantitative proteomics by mass spectrometry.
Download or read book Synthesis and Applications of Isotopically Labelled Compounds 1997 written by J. R. Heys and published by . This book was released on 1998-06-11 with total page 776 pages. Available in PDF, EPUB and Kindle. Book excerpt: Synthesis and Applications of Isotopically Labelled Compounds provides a wide-ranging, authoritative and comprehensive coverage of the most recent advances in the synthesis, production and applications of stable and radioactive isotopes. This volume focuses on significant growth areas including applications of isotopes in clinical research and medicine, advances in the analysis of isotopically labelled compounds, novel approaches to compounds labelled with isotopes of hydrogen, applications of isotopes in organic and bio-organic reaction mechanisms, isotopes in macro-molecules, applications of isotopes in metabolism and toxicology, production of stable isotopes and radioisotopes and applications of isotopes in pharmaceutical and agricultural research. Contributions are drawn from major research groups around the world providing an invaluable information and reference source to anyone involved in the production of isotopes, the synthesis and analysis of stable or radioactive isotopically labelled compounds, the applications of short-lived isotopes, drug metabolism and pharmacokinetics, pharmaceutical research and development, clinical research, biosynthesis, handling radioisotopes, laboratory design and other related areas.
Download or read book Synthesis of Violacein for Stable Isotopic Labeling by Amino Acids in Cell Culture written by Awura Ama A Oppong-Holmes and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Violacein is an intensely purple microbial pigment with an arsenal of biological activity. A structural analog to the better-known bis-indole alkaloids staurosporine and rebeccamycin, violacein has been touted as an antimicrobial and anticancer therapeutic. It is most frequently obtained as a biosynthetic secondary metabolite from bacterial cell cultures. However, we have developed an efficient, environmentally benign methodology for synthesizing the natural product via a 3-component condensation in polyethylene glycol. Violacein, along with a series of analogs have been prepared in our laboratory. The deoxyviolacein analog has been investigated for its effects against cancer cell migration in HeLa. This initial screening has served as the impetus to study deoxyviolacein's specific interactions in cancer cells via proteomics. In this project, the synthesis of the deoxyviolacein scaffold for solid support is designed, in preparation for SILAC (Stable Isotope Labeling by Amino acids in Cell culture) analysis. The synthesis requires preparation of 5-hydroxymethyl-1H-indole, reported here. The main objective of this project is to design and synthesize a functional analog that can be used to determine the protein interactions responsible for the biological activity of violacein.
Download or read book Isotope Tracers in Metabolic Research written by Robert R. Wolfe and published by John Wiley & Sons. This book was released on 2004-10-18 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past few years, the number of applications of tracers for in vivo biomedical studies has greatly increased. New analytical tools at the genetic and protein levels have spurred this growth, opening the door for a deeper understanding of metabolic events. This in turn promises to yield significant advances in the understanding and treatment of human disease. Now fully revised and expanded, Isotope Tracers in Metabolic Research, Second Edition is the established definitive text on stable and radioactive isotope tracers. In unique, multidisciplinary fashion, it presents comprehensive coverage of new methodological, mathematical, and theoretical approaches. This new Second Edition includes: All-new chapters on nuclear magnetic resonance, mass isotopomer analysis, and methods of protein metabolism analysis A completely updated categorized list of over 750 references Major advances in the development of mass isotopomer and positional isotopomer techniques, noninvasive isotope techniques for studying metabolic pathways, hyphenated techniques, and new tracer techniques The latest developments in quantification of DNA synthesis and mass spectrometry spurred by genome sequencing and proteomics New coverage of mathematical modeling Expanded coverage of microdialysis probes, laboratory procedures, and regulatory issues related to human studies In this complete guide to performing tracer studies, the authors systematically cover tracer selection, modeling considerations, sample derivitization, mass spectrometry analysis, and data interpretation. Problems and discussion questions highlight key points in each chapter. Isotope Tracers in Metabolic Research, Second Edition offers students and researchers a comprehensive, practical resource for utilizing the latest tracer methodologies.
Download or read book Noncanonical Amino Acids in the Interrogation of Cellular Protein Synthesis written by John Tuan Ngo and published by . This book was released on 2012 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins in living cells can be made receptive to bioorthogonal chemistries through metabolic labeling with appropriately designed noncanonical amino acids (ncAAs). In the simplest approach to metabolic labeling, an amino acid analog replaces one of the natural amino acids specified by the protein's gene (or genes) of interest. This approach, often termed "residue-specific incorporation," allows the ncAA to be incorporated in controlled proportions into positions normally occupied by the natural amino acid residue. Chapter I of this thesis describes how this strategy has been used to track cellular protein synthesis with reactive ncAAs. In procedures similar to isotopic labeling, translationally active ncAAs are incorporated into proteins during a "pulse" in which newly synthesized proteins are tagged. The set of tagged proteins can be distinguished from those made before the pulse by bioorthogonally ligating the ncAA side chain to probes that permit detection, isolation, and visualization of the labeled proteins. Chapter II of this thesis describes how the selectivity of the method can be enhanced through the use of mutant aminoacyl tRNA synthetases (aaRSs) that permit incorporation of ncAAs not used by the endogenous biomachinery. Expression of a mutant synthetase in a portion of cells within a complex cellular mixture restricts labeling to that subset of cells. In multicellular environments, this approach permits the identification of the cellular origins of labeled proteins. The work in Chapter III illustrates how the extent of temporal and spatial resolution of protein labeling can be enhanced through controlled expression of mutant synthetases. Use of characterized promoters to direct transcription of mutant synthetase genes can limit labeling to relevant cells and physiological states in settings of increased complexity. Chapter IV presents a novel strategy with which ncAAs can be uniquely incorporated at the N-terminal positions of nascent proteins while excluded from insertion at internal positions. This approach permits "site-selective" tagging of cellular proteins, and its use in tagging and visualization of cell-cycle dependent protein synthesis is described. The work described throughout this thesis was designed with the objective of providing powerful and versatile methods for the study of protein synthesis in complex multicellular systems, including live animals. Thus, Chapter V considers how these strategies might be used to dissect protein synthesis in living animals.
Download or read book Synthesis and Applications of Isotopically Labelled Compounds 1988 written by Thomas A. Baillie and published by Elsevier Publishing Company. This book was released on 1989 with total page 874 pages. Available in PDF, EPUB and Kindle. Book excerpt:
