Download or read book The Ras PKA Pathway Controls Transcription of Genes Involved in Stationary Phase Entry in Saccharomyces Cerevisiae written by Ya-Wen Chang and published by . This book was released on 2003 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: Upon nutrient deprivation, Saccharomyces cerevisiae cells arrest division and enter into a specialized resting state, known as stationary phase. The entry into this resting state is regulated, in part, by the Ras/PKA (cAMP-dependent protein kinase) signaling pathway. We are interested in understanding the mechanisms regulating stationary phase biology in S. cerevisiae, with an ultimate goal of defining the targets of PKA that are responsible for this growth control. To this end, we have identified a collection of mutants that exhibited a defective transcriptional response to nutrient limitation and failed to enter into a normal stationary phase. These rye mutants were isolated on the basis of defects in the regulation of YGP1 expression. We found that the levels of YGP1 were significantly elevated in the rye mutants during the log phase of growth. The rye defects were not specific to this YGP1 expression defect because these mutants also exhibited multiple defects in stationary phase properties, including an inability to survive periods of prolonged starvation and a failure to tolerate a mild heat shock. These data indicated that the RYE genes may encode important regulators of yeast cell growth. Interestingly, three of these RYE genes encoded the Srb proteins, Srb9p, Srb10p, and Srb11p. These Srb proteins are components of the Srb complex associated with the RNA polymerase II holoenzyme. We found that specific transcription defects associated with these srb mutations were suppressed by RAS2val19, a hyperactive allele of RAS2. However, increased Ras signaling was not able to correct the expression defects associated with an srb9 null mutant, suggesting that the Srb9 protein is essential for the Ras suppression. Moreover, there are two potential PKA consensus sites in Srb9p. Our results showed that the suppression of the srb9 defects required the presence of these two PKA sites. In addition, we have found that Srb9p was phosphorylated by PKA in vitro and in vivo. In all, our results suggest that Srb9p is a substrate for PKA, and that this phosphorylation of Srb9p modulates the in vivo activity of the Srb complex to regulate transcription of a subset of genes involved in stationary phase entry.
Download or read book The RAS PKA Signaling Pathway in Saccharomyces Cerevisiae May Regulate the Function of Proteins Associated with the RPB1P CTD to Coordinate Transcription with Nutrient Availability written by Susie C. Howard and published by . This book was released on 2002 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Ras PKA Signaling Pathway Regulates Growth in Response to Nutrient Availability in S Cerevisiae Coordinately with the Ror Pathway written by Vidhya Ramachandran and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: In addition to post-translational regulation, cells respond to changing nutrient levels by modulating mRNA turnover in the cell, to regulate gene expression as well as translation. In the recent years, the mRNP aggregates known as P-bodies have emerged as a major site and player in mRNA decapping and decay. Very little is known about the regulation of P-body formation in response to nutrient deprivation. To this end, our study in Chapter 3 describes a novel aspect of P-body biology, wherein PKA targets the P-body component Pat1, in response to nutrient deprivation, to regulate P-body formation in S. cerevisiae. Thus, Ras/PKA signaling might constitute a link between nutrient levels and mRNA turnover facilitated by P-bodies.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 2004 with total page 882 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Signaling in Plants written by František Baluška and published by Springer Science & Business Media. This book was released on 2009-02-27 with total page 307 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first comprehensive monograph on all emerging topics in plant signaling. The book addresses diverse aspects of signaling at all levels of plant organization. Emphasis is placed on the integrative aspects of signaling.
Download or read book The Molecular Biology of the Yeast Saccharomyces Life Cycle and Inheritance written by Jeffrey N. Strathern and published by . This book was released on 1981 with total page 776 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Mitochondrial Pathogenesis written by Hong Kyu Lee and published by Springer. This book was released on 2014-01-10 with total page 361 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Metabolism and Molecular Physiology of Saccharomyces Cerevisiae written by J. Richard Dickinson and published by CRC Press. This book was released on 2004-04-27 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the publication of the best-selling first edition, much has been discovered about Saccharomyces cerevisiae, the single-celled fungus commonly known as baker's yeast or brewer's yeast that is the basis for much of our understanding of the molecular and cellular biology of eukaryotes. This wealth of new research data demands our attention and r
Download or read book Yeast Membrane Transport written by José Ramos and published by Springer. This book was released on 2015-12-31 with total page 381 pages. Available in PDF, EPUB and Kindle. Book excerpt: This contributed volume reviews the recent progress in our understanding of membrane transport in yeast including both Saccharomyces cerevisiae and non-conventional yeasts. The articles provide a summary of the key transport processes and put these in a systems biology context of cellular regulation, signal reception and homeostasis. After a general introduction, readers will find review articles covering the mechanisms and regulation of transport for various substrates ranging from diverse nutrients to cations, water and protons. These articles are complemented by a chapter on extremophilic yeast, a chapter on the mathematical modelling of ion transport and two chapters on the role of transport in pathogenic yeasts and antifungal drug resistance. Each article provides both a general overview of the main transport characteristics of a specific substrate or group of substrates and the unique details that only an expert working in the field is able to transmit to the reader. Researchers and students of the topic will find this book to be a useful resource for membrane transport in yeast collecting information in one complete volume, which is otherwise scattered across many papers. This might also be interesting for scientists investigating other species in order to compare transport mechanisms with known functions in yeast with the cells on which they work.
Download or read book Methods in Yeast Genetics written by David C. Amberg and published by CSHL Press. This book was released on 2005 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Methods in Yeast Genetics" is a course that has been offered annually at Cold Spring Harbor for the last 30 years. This provides a set of teaching experiments along with the protocols and recipes for the standard techniques and reagents used in the study of yeast biology.
Download or read book Index Medicus written by and published by . This book was released on 2004 with total page 1812 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biochemistry of Beer Fermentation written by Eduardo Pires and published by Springer. This book was released on 2015-02-09 with total page 85 pages. Available in PDF, EPUB and Kindle. Book excerpt: Beer is the most popular alcoholic beverage in the world. Yet, behind each glass of beer there is an enormous amount of work invested. If the first image that comes to your mind is the lifting of heavy bags of malt or carrying kegs, guess again! Most of the work involved in brewing is carried out by “microworkers” – yeast and their enzymes! These special helpers are responsible for catalyzing the vast majority of the biochemical reactions occurring in all steps that gradually transform the sugary wort into beer. This book not only provides readers with an overview of the whole biochemical process involved in beer fermentation, but also reviews the latest findings in this delightful field, making it essential reading for both scientists and brewing enthusiasts
Download or read book Systems Biology written by Lilia Alberghina and published by Springer Science & Business Media. This book was released on 2007-10-04 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: For life to be understood and disease to become manageable, the wealth of postgenomic data now needs to be made dynamic. This development requires systems biology, integrating computational models for cells and organisms in health and disease; quantitative experiments (high-throughput, genome-wide, living cell, in silico); and new concepts and principles concerning interactions. This book defines the new field of systems biology and discusses the most efficient experimental and computational strategies. The benefits for industry, such as the new network-based drug-target design validation, and testing, are also presented.
Download or read book Control Theory and Systems Biology written by Pablo A. Iglesias and published by MIT Press. This book was released on 2010 with total page 359 pages. Available in PDF, EPUB and Kindle. Book excerpt: A survey of how engineering techniques from control and systems theory can be used to help biologists understand the behavior of cellular systems.
Download or read book Protein Kinase CK2 From Structure to Regulation written by Khalil Ahmed and published by Springer Science & Business Media. This book was released on 2002-06-30 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt: Signal Transduction through phosphorylation and dephosphorylation of proteins in the cell is now a well-recognized mechanism involved in countless physiological and pathological processes. Consequently, the enzymes, known as protein kinases, which catalyze the phosphorylation of proteins, are critical regulators of cellular events. One of these protein kinases is the protein kinase CK2 (also known as casein kinase 2) that has been implicated in multiple functions including control of cell growth and proliferation. CK2 is a protein serine/threonine kinase which is a highly conserved and ubiquitous protein kinase. It is localized in the cytoplasmic and nuclear compartments, which accords with its multiple functional activities in the cell. Pertinent to this is also the recognition that a large number of putative substrates for this kinase have been identified in various compartments of the cell. New evidence from several laboratories has further reinforced the involvement of CK2 in signal transduction related to many cellular functions, thus underscoring the significance of its functional role in normal and abnormal cell growth and proliferation. This volume provides an overview of the state of knowledge concerning this intriguing protein kinase. It brings together contributions from leading investigators engaged in research in this field. Key developments during the past three years pertain to the elaboration of the crystal structure and definition of novel functions of the kinase, such as its role as an inhibitor of apoptosis. Additionally, the shuttling of the kinase to various compartments in response to physiological and stress stimuli appears to be a key feature of the functional regulation of its activity in the cell.
Download or read book Biological Aging written by Trygve O. Tollefsbol and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 844 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book investigates the various processes that are affected by the age of an organism. Several new tools for the analysis of biological aging have been introduced recently, and this volume provides methods and protocols for these new techniques in addition to its coverage of established procedures. Researchers seeking new technology and techniques will find this volume of tremendous benefit as they move towards new directions.
Download or read book Guide to Yeast Genetics and Molecular Biology written by Christine Guthrie and published by . This book was released on 1991-01-28 with total page 933 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to Yeast Genetics and Molecular Biology presents, for the first time, a comprehensive compilation of the protocols and procedures that have made Saccharomyces cerevisiae such a facile system for all researchers in molecular and cell biology. Whether you are an established yeast biologist or a newcomer to the field, this volume contains all the up-to-date methods you will need to study "Your Favorite Gene" in yeast. Basic Methods in Yeast Genetics**Physical and genetic mapping**Making and recovering mutants**Cloning and Recombinant DNA Methods**High-efficiency transformation**Preparation of yeast artificial chromosome vectors**Basic Methods of Cell Biology**Immunomicroscopy**Protein targeting assays**Biochemistry of Gene Expression**Vectors for regulated expression**Isolation of labeled and unlabeled DNA, RNA, and protein
