Download or read book The Protein Protocols Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2008-02-12 with total page 759 pages. Available in PDF, EPUB and Kindle. Book excerpt: In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose.
Download or read book The Protein Protocols Handbook written by John M. Walker and published by Springer. This book was released on 2007-10-02 with total page 1146 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.
Download or read book The Protein Protocols Handbook 2E written by Walker and published by . This book was released on 2007-09-01 with total page 1176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Proteomics Protocols Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2007-10-09 with total page 969 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Download or read book Protein Purification Protocols written by Paul Cutler and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of Protein Purification Protocols (1996), edited by Professor Shawn Doonan, rapidly became very successful. Professor Doonan achieved his aims of p- ducing a list of protocols that were invaluable to newcomers in protein purification and of significant benefit to established practitioners. Each chapter was written by an ex- rienced expert in the field. In the intervening time, a number of advances have w- ranted a second edition. However, in attempting to encompass the recent developments in several areas, the intention has been to expand on the original format, retaining the concepts that made the initial edition so successful. This is reflected in the structure of this second edition. I am indebted to Professor Doonan for his involvement in this new edition and the continuity that this brings. Each chapter that appeared in the original volume has been reviewed and updated to reflect advances and bring the topic into the 21st century. In many cases, this reflects new applications or new matrices available from vendors. Many of these have increased the performance and/or scope of the given method. Several new chapters have been introduced, including chapters on all the currently used protein fractionation and ch- matographic techniques. They introduce the theory and background for each method, providing lists of the equipment and reagents required for their successful execution, as well as a detailed description of how each is performed.
Download or read book Basic Protein and Peptide Protocols written by John M. Walker and published by Humana. This book was released on 1994-04-26 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Protein and Peptide Protocols offers an excellent collection of reproducible, step-by-step laboratory methods covering three major areas: (1) the quantitation and characterization of proteins, (2) the electrophoretic and blotting procedures used in protein isolation and characterization, and (3) the analysis of protein and peptide structure. THOUSANDS of labs are already using Basic Protein and Peptide Protocols-you should be too!
Download or read book Protein Engineering Protocols written by Kristian Müller and published by Springer Science & Business Media. This book was released on 2007-10-26 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Protein Engineering Protocols will consider the two general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The s- ond strategy is known as directed evolution. In this case, random mutagenesis is applied to a protein, and selection or screening is used to pick out variants that have the desired qualities. By several rounds of mutation and selection, this method mimics natural evolution. An additional technique known as DNA shuffling mixes and matches pieces of successful variants to produce better results. This process mimics recombination that occurs naturally during sexual reproduction. The first section of Protein Engineering Protocols describes rational p- tein design strategies, including computational methods, the use of non-natural amino acids to expand the biological alphabet, as well as impressive examples for the generation of proteins with novel characteristics. Although procedures for the introduction of mutations have become routine, predicting and und- standing the effects of these mutations can be very challenging and requires profound knowledge of the system as well as protein structures in general.
Download or read book Molecular Biomethods Handbook written by John M. Walker and published by Springer Science & Business Media. This book was released on 2008-11-04 with total page 1105 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
Download or read book Cell free Protein Synthesis written by Alexander S. Spirin and published by John Wiley & Sons. This book was released on 2007-12-03 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.
Download or read book Protein Structure Prediction written by David Webster and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: The number of protein sequences grows each year, yet the number of structures deposited in the Protein Data Bank remains relatively small. The importance of protein structure prediction cannot be overemphasized, and this volume is a timely addition to the literature in this field. Protein Structure Prediction: Methods and Protocols is a departure from the normal Methods in Molecular Biology series format. By its very nature, protein structure prediction demands that there be a greater mix of theoretical and practical aspects than is normally seen in this series. This book is aimed at both the novice and the experienced researcher who wish for detailed inf- mation in the field of protein structure prediction; a major intention here is to include important information that is needed in the day-to-day work of a research scientist, important information that is not always decipherable in scientific literature. Protein Structure Prediction: Methods and Protocols covers the topic of protein structure prediction in an eclectic fashion, detailing aspects of pred- tion that range from sequence analysis (a starting point for many algorithms) to secondary and tertiary methods, on into the prediction of docked complexes (an essential point in order to fully understand biological function). As this volume progresses, the authors contribute their expert knowledge of protein structure prediction to many disciplines, such as the identification of motifs and domains, the comparative modeling of proteins, and ab initio approaches to protein loop, side chain, and protein prediction.
Download or read book HPLC of Peptides and Proteins written by Marie-Isabel Aguilar and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.
Download or read book A Manual for Biochemistry Protocols written by Markus R. Wenk and published by World Scientific. This book was released on 2007 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biochemistry plays an important role in all areas of the biological and medical sciences. With most of the research or diagnosis involved in these areas being based on biochemically obtained observations, it is essential to have a profile of well standardized protocols.This manual is a basic guide for all students, researchers and experts in biochemistry, designed to help readers in directly starting off their experiments without prior knowledge of the protocol. The book dwells on the concepts used in designing the methodologies, thereby giving ample room for researchers to modify them according to their research requirements.
Download or read book Pichia Protocols written by James M Cregg and published by Springer Science & Business Media. This book was released on 2007-08-08 with total page 553 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.
Download or read book Flow Cytometry Protocols written by Teresa S. Hawley and published by Springer Nature. This book was released on with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Practical Protein Crystallography written by Duncan E. McRee and published by Academic Press. This book was released on 2012-12-02 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: Designed for easy use by both new and experienced protein crystallographers, this much-needed book is for anyone interested in solving protein structures by the method of crystallography. It contains many examples ofactual experiments and data, including electron density maps. Computer methods and computer code samples are presented. Practical Protein Crystallography is loaded with new information on area detectors, synchrotron radiation techniques, and the latest computer methods, and features the XtalView software system. Graduate students and teachers in physical biochemistry and pharmaceutical researchers will find this text a timely and convenient aid.
Download or read book Protein Purification written by Philip L.R. Bonner and published by Garland Science. This book was released on 2007-06-30 with total page 201 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein Purification provides a guide to the major techniques, including non-affinity absorption techniques, affinity procedures, non-absorption techniques and methods for monitoring protein purity. There is an overview of protein strategy and equipment, followed by discussions and examples of each technique and its applications. The basic theory and simple explanations given in Protein Purification make it an ideal handbook for final year undergraduates, and postgraduates, who are conducting research projects. It will also be a useful guide to more experienced researchers who need a good overview of the techniques and products used in protein purification.
Download or read book Proteins written by David Whitford and published by John Wiley & Sons. This book was released on 2013-04-25 with total page 544 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins: Structure and Function is a comprehensive introduction to the study of proteins and their importance to modern biochemistry. Each chapter addresses the structure and function of proteins with a definitive theme designed to enhance student understanding. Opening with a brief historical overview of the subject the book moves on to discuss the ‘building blocks’ of proteins and their respective chemical and physical properties. Later chapters explore experimental and computational methods of comparing proteins, methods of protein purification and protein folding and stability. The latest developments in the field are included and key concepts introduced in a user-friendly way to ensure that students are able to grasp the essentials before moving on to more advanced study and analysis of proteins. An invaluable resource for students of Biochemistry, Molecular Biology, Medicine and Chemistry providing a modern approach to the subject of Proteins.
