Download or read book The Involvement of RNA in the Initiation of DNA Replication written by Leonard Charles Erickson and published by . This book was released on 1974 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Initiation of DNA Replication written by Dan S Ray and published by Elsevier. This book was released on 2012-12-02 with total page 649 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Initiation of DNA Replication contains the proceedings of the 1981 ICN-UCLA Symposia on Structure and DNA-Protein Interactions of Replication Origins, held in Salt Lake City, Utah on March 8-13, 1981. The papers explore the initiation of DNA replication and address relevant topics such as whether there are specific protein recognition sites within an origin; how many proteins interact at an origin and whether they interact in a specific temporal sequence; or whether origins can be subdivided into distinct functional domains. The specific biochemical steps in DNA chain initiation and how they are catalyzed are also discussed. This book is organized into six sections and comprised of 41 chapters. The discussion begins by analyzing the replication origin region of the Escherichia coli chromosome and the precise location of the region carrying autonomous replicating function. A genetic map of the replication and incompatibility regions of the resistance plasmids R100 and R1 is described, and several gene products produced in vivo or in vitro from the replication region are considered. The sections that follow focus on the DNA initiation determinants of bacteriophage M13 and of chimeric derivatives carrying foreign replication determinants; suppressor loci in E. coli; and enzymes and proteins involved in initiation of phage and bacterial chromosomes. The final chapters examine the origins of eukaryotic replication. This book will be of interest to scientists, students, and researchers in fields ranging from microbiology and molecular biology to biochemistry, molecular genetics, and physiology.

Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Proteins Involved in DNA Replication written by Ulrich Huebscher and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book collects the Proceedings of a workshop sponsored by the European Molecular Biology Organization (EMBO) entitled "Pro teins Involved in DNA Replication" which was held September 19 to 23,1983 at Vitznau, near Lucerne, in Switzerland. The aim of this workshop was to review and discuss the status of our knowledge on the intricate array of enzymes and proteins that allow the replication of the DNA. Since the first discovery of a DNA polymerase in Escherichia coli by Arthur Kornberg twenty eight years ago, a great number of enzymes and other proteins were des cribed that are essential for this process: different DNA poly merases, DNA primases, DNA dependent ATPases, helicases, DNA liga ses, DNA topoisomerases, exo- and endonucleases, DNA binding pro teins and others. They are required for the initiation of a round of synthesis at each replication origin, for the progress of the growing fork, for the disentanglement of the replication product, or for assuring the fidelity of the replication process. The number, variety and ways in which these proteins inter act with DNA and with each other to the achievement of replication and to the maintenance of the physiological structure of the chromo somes is the subject of the contributions collected in this volume. The presentations and discussions during this workshop reinforced the view that DNA replication in vivo can only be achieved through the cooperation of a high number of enzymes, proteins and other cofactors.

Download or read book Mechanism and Regulation of DNA Replication written by Alan Kolber and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 457 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1 - Chromosome Replication in Procaryotes.- Enzymatic Aspects of Chromosome Replication in E. coli.- Escherichia coli DNA Polymerase II and III.- Initiation of DNA Synthesis.- In vitro Replication of DNA.- The Role of ATP in Chromosome Replication Studied in Toluenized Escherichia coli.- Membrane Protein Components and DNA Synthesis in Escherichia coli.- A Possible Common Role for DNA Polymerase I and Exonuclease V in Escherichia coli.- The Joining of DNA Duplexes at Their Base-Paired Ends.- The Attachment of the Bacterial Chromosome to the Cell Membrane.- DNA Replication in Bacteriophage and.

Download or read book DNA Replication written by Hisao Masai and published by Springer. This book was released on 2018-01-22 with total page 581 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews the latest trends and future directions of DNA replication research. The contents reflect upon the principles that have been established through the genetic and enzymatic studies of bacterial, viral, and cellular replication during the past decades. The book begins with a historical overview of the studies on eukaryotic DNA replication by Professor Thomas Kelly, a pioneer of the field. The following chapters include genome-wide studies of replication origins and initiation factor binding, as well as the timing of DNA replications, mechanisms of initiation, DNA chain elongation and termination of DNA replication, the structural basis of functions of protein complexes responsible for execution of DNA replication, cell cycle-dependent regulation of DNA replication, the nature of replication stress and cells’ strategy to deal with the stress, and finally how all these phenomena are interconnected to genome instability and development of various diseases. By reviewing the existing concepts ranging from the old principles to the newest ideas, the book gives readers an opportunity to learn how the classical replication principles are now being modified and new concepts are being generated to explain how genome DNA replication is achieved with such high adaptability and plasticity. With the development of new methods including cryoelectron microscopy analyses of huge protein complexes, single molecular analyses of initiation and elongation of DNA replication, and total reconstitution of eukaryotic DNA replication with purified factors, the field is enjoying one of its most exciting moments, and this highly timely book conveys that excitement to all interested readers.

Download or read book DNA Replication in Eukaryotic Cells written by Melvin L. DePamphilis and published by CSHL Press. This book was released on 1996 with total page 1080 pages. Available in PDF, EPUB and Kindle. Book excerpt: National Institutes of Health. Cold Spring Harbor Monograph, Volume 31 Extensive text on the replication of DNA, specifically in eukaryotic cells, for researchers. 68 contributors, 54 U.S.

Download or read book Concepts of Biology written by Samantha Fowler and published by . This book was released on 2018-01-07 with total page 618 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concepts of Biology is designed for the single-semester introduction to biology course for non-science majors, which for many students is their only college-level science course. As such, this course represents an important opportunity for students to develop the necessary knowledge, tools, and skills to make informed decisions as they continue with their lives. Rather than being mired down with facts and vocabulary, the typical non-science major student needs information presented in a way that is easy to read and understand. Even more importantly, the content should be meaningful. Students do much better when they understand why biology is relevant to their everyday lives. For these reasons, Concepts of Biology is grounded on an evolutionary basis and includes exciting features that highlight careers in the biological sciences and everyday applications of the concepts at hand.We also strive to show the interconnectedness of topics within this extremely broad discipline. In order to meet the needs of today's instructors and students, we maintain the overall organization and coverage found in most syllabi for this course. A strength of Concepts of Biology is that instructors can customize the book, adapting it to the approach that works best in their classroom. Concepts of Biology also includes an innovative art program that incorporates critical thinking and clicker questions to help students understand--and apply--key concepts.

Download or read book Growth Rate Regulation and Control of Initiation of DNA Replication in E s c h e r i c h i a C o l i written by and published by . This book was released on 1990 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: The initiation of DNA synthesis at the chromosomal replication origin, oriC, in Escherichia coli involves an RNA polymerase-mediated step. The level of synthesis of transcripts moving counterclockwise toward oriC is controlled at two promoters, P1 (asnC) and P2 (mioC), and at two transcription terminator regions, T1 and T2. As shown by S1 mapping, termination at the T2 region occurs to the right of oriC at nucleotides 297-299 and 306-310, while major termination events in the T1 region occur in and near the mioC promoter. The majority of transcripts entering oriC originates from the mioC promoter. Transcription from the mioC promoter has been shown to enhance the frequency of initiation of DNA replication of oriC containing plasmids, and to stabilize these plasmids in the host cells. The mioC promoter, which is stringently controlled, is also growth rate regulated. The amount of mioC transcripts relative to the amount of total RNA was inversely correlated with growth rate. This transcript is characterized by a short half-life (1.5 min). The mioC promoter, which contains a DnaA protein binding site, was much less susceptible to repression by DnaA protein when located in the chromosome, than when located in a plasmid. Only a very high concentration of DnaA protein repressed the mioC promoter. The DnaA protein, which is required for initiation of DNA replication from oriC, is growth rate regulated. As shown by RNase protection, this regulation is exerted at the transcriptional level, affecting both promoters, dnaAp1 and dnaAp2. Transcription from these two promoters is also stringently controlled. The amount of DnaA protein in spoT mutants, which are deficient in ppGpp pyrophosphorylase activity, decreases as the severity in the mutation increases. Thus, the intracellular concentration of ppGpp influences the expression of the dnaA gene. In conclusion, the growth rate regulation and stringent control of the dnaA gene suggest that one way in which DNA replication is coordinated with the growth rate is via ppGpp synthesis at the ribosome.

Download or read book Growth Rate Regulation and Control of Initiation of DNA Replication in Escherichia Coli written by Anne Eliane Chiaramello and published by . This book was released on 1990 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: The initiation of DNA synthesis at the chromosomal replication origin, oriC, in Escherichia coli involves an RNA polymerase-mediated step. The level of synthesis of transcripts moving counterclockwise toward oriC is controlled at two promoters, P1 (asnC) and P2 (mioC), and at two transcription terminator regions, T1 and T2. As shown by S1 mapping, termination at the T2 region occurs to the right of oriC at nucleotides 297-299 and 306-310, while major termination events in the T1 region occur in and near the mioC promoter. The majority of transcripts entering oriC originates from the mioC promoter. Transcription from the mioC promoter has been shown to enhance the frequency of initiation of DNA replication of oriC containing plasmids, and to stabilize these plasmids in the host cells. The mioC promoter, which is stringently controlled, is also growth rate regulated. The amount of mioC transcripts relative to the amount of total RNA was inversely correlated with growth rate. This transcript is characterized by a short half-life (1.5 min). The mioC promoter, which contains a DnaA protein binding site, was much less susceptible to repression by DnaA protein when located in the chromosome, than when located in a plasmid. Only a very high concentration of DnaA protein repressed the mioC promoter. The DnaA protein, which is required for initiation of DNA replication from oriC, is growth rate regulated. As shown by RNase protection, this regulation is exerted at the transcriptional level, affecting both promoters, dnaAp1 and dnaAp2. Transcription from these two promoters is also stringently controlled. The amount of DnaA protein in spoT mutants, which are deficient in ppGpp pyrophosphorylase activity, decreases as the severity in the mutation increases. Thus, the intracellular concentration of ppGpp influences the expression of the dnaA gene. In conclusion, the growth rate regulation and stringent control of the dnaA gene suggest that one way in which DNA replication is coordinated with the growth rate is via ppGpp synthesis at the ribosome.

Download or read book Transcription Factors and DNA Replication written by David Scott Pederson and published by R. G. Landes. This book was released on 1994 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt: Initiation of DNA synthesis: a general model from studies with prokaryotes. Regulatory transcription factors and the initiation of transcription in eucaryotic cells. Replication origins and the initiation of DNA synthesis in mammalian cells and the viruses. DNA replication in yeast. Transcription factors and control of DNA replication.

Download or read book Dna Replication In Plants written by John A. Bryant and published by CRC Press. This book was released on 2018-01-18 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: This texts discusses DNA replication in plants including chapters on; functional chromosomal structure, the biochemistry of DNA replication, Control of DNA replication, Replication of plant organelle DNA, replication of DNA viruses in plants, and DNA damage, repair, and mutagenesis.

Download or read book DNA Protein Interactions written by A. Travers and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt: Our understanding of the mechanisms regulating gene expression, which determine the patterns of growth and development in all living organisms, ultimately involves the elucidation of the detailed and dy namic interactions of proteins with nucleic acids -both DNA and RNA. Until recently the commonly presented view of the DNA double helix as visualized on the covers of many textbooks and journals - was as a monotonous static straight rod incapable in its own right of directing the processes necessary for the conservation and selective reading of genetic information. This view, although perhaps extreme, was reinforced by the necessary linearity of genetic maps. The reality is that the biological functions of both DNA and RNA are dependent on complex, and sometimes transient, three-dimensional nucleoprotein structures in which genetically distant elements are brought into close spatial proximity. It is in such structures that the enzymatic manipulation of DNA in the essential biological processes as DNA replication, transcription and recombination are effected - the complexes are the mediators of the 'DNA transactions' of Hatch Echols.

Download or read book DNA Replication written by Roger Lionel Poulter Adams and published by Oxford University Press, USA. This book was released on 1991 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt: 'In Focus' is a series of books specifically written for students facing the problem of keeping up to date with key areas in biology and medicine. Each title presents the very latest information in a clear and accessible format. These book will particularly complement course work, providing an in-depth knowledge of the topic.

Download or read book Telomeres and Telomerase written by CIBA Foundation Symposium and published by Wiley. This book was released on 1998-03-11 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: Telomeres and Telomerase Chairman: Sydney Brenner, 1997 Telomeres are the protective genetic elements located at the ends of chromosomes and are essential for correct chromosomal structure and function. They are not fully replicated by the conventional DNA polymerase system because DNA synthesis occurs only in the 5' to 3' direction and requires an RNA primer for initiation. Consequently, cells require a special enzyme to maintain the telomeric ends of chromosomes during each round of replication. This enzyme, telomerase, is a ribonucleoprotein that extends chromosome ends by adding short stretches of nucleotide repeats using a portion of its integral RNA component as the template. Recently, much excitement has been generated by the suggestion that telomerase, or rather the absence of telomerase and the resultant loss of terminal DNA, is a cause of human ageing. The evidence for this is twofold: the telomeres of certain cells in culture shorten during their lifespan; and immortalization of cells is associated, at least in some cases, with the maintenance of telomeres and telomerase activity. The latter observation prompted the analysis of clinical samples from patients with cancer and the demonstration that, in contrast to normal somatic cells, malignant cells possess telomerase activity. This is a unique book. Not only does it contain the latest experimental results from an international group of experts, but it also includes critical examinations of the current evidence, and discussions that attempt to identify the central and underlying concepts of this rapidly expanding field.

Download or read book Microbiology written by Nina Parker and published by . This book was released on 2016-05-30 with total page 1301 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology's art program enhances students' understanding of concepts through clear and effective illustrations, diagrams, and photographs. Microbiology is produced through a collaborative publishing agreement between OpenStax and the American Society for Microbiology Press. The book aligns with the curriculum guidelines of the American Society for Microbiology."--BC Campus website.

Download or read book Biology for AP Courses written by Julianne Zedalis and published by . This book was released on 2017-10-16 with total page 1923 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biology for AP® courses covers the scope and sequence requirements of a typical two-semester Advanced Placement® biology course. The text provides comprehensive coverage of foundational research and core biology concepts through an evolutionary lens. Biology for AP® Courses was designed to meet and exceed the requirements of the College Board’s AP® Biology framework while allowing significant flexibility for instructors. Each section of the book includes an introduction based on the AP® curriculum and includes rich features that engage students in scientific practice and AP® test preparation; it also highlights careers and research opportunities in biological sciences.