Download or read book The Genetic Analysis of the Methylotrophic Yeast Hansenula Polymorpha written by Martin Antony Gerrard Gleeson and published by . This book was released on 1986 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Hansenula Polymorpha written by Gerd Gellissen and published by Wiley-Blackwell. This book was released on 2002-06-10 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methylotrophic yeasts have attracted increasing interest as useful systems for fundamental research and applied purposes. Hansenula polymorpha in particular has become a preferred organism for the production of recombinant proteins on an industrial scale. Product examples range from therapeutics such as hepatitis B vaccines to industrial enzymes like the feed additive phytase. This book is addressed to researchers and scientists working in the field and provides a comprehensive, up-to-date overview of the present status of Hansenula polymorpha research, applications and methods. Aspects of the organism ranging from systematics, genetics, methanol metabolism and peroxisomal function to its use as a technology platform for the production of recombinant proteins are covered. A detailed chapter on laboratory methods is also included.
Download or read book Hansenula Polymorpha written by Gerd Gellissen and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text is addressed to reseachers and scientists working in the field with methylotrophic yeasts, in particular hansenula polymorpha, and provides a comphrehensive overview of the present status of hensenula polymorpha research, applications and methods. Aspects of the organism ranging from systematics, genetics, methanol metabolism and peroxisomal function to its use as a technology platform for the production of recombinant proteins are covered. A detailed chapter on laboratory methods is also included.
Download or read book Production of Recombinant Proteins written by Gerd Gellissen and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: While the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial settings do not have ready access to pertinent biological and technical information since it is normally scattered throughout the scientific literature. This book closes the gap by providing information on the general biology of the host organism, a description of the expression platform, a methodological section -- with strains, genetic elements, vectors and special methods, where applicable -- as well as examples of proteins produced with the respective platform. The systems thus described are well balanced by the inclusion of three prokaryotes (two Gram-negatives and one Gram-positive), four yeasts, two filamentous fungi and two higher eukaryotic cell systems -- mammalian and plant cells. Throughout, the book provides valuable practical and theoretical information on the criteria and schemes for selecting the appropriate expression platform, the possibility and practicality of a universal expression vector, and on comparative industrial-scale fermentation, with the production of a recombinant Hepatitis B vaccine chosen as an industrial example. With a foreword by Herbert P. Schweizer, Colorado State University, USA: "As a whole, this book is a valuable and overdue resource for a varied audience. It is a practical guide for academic and industrial researchers who are confronted with the design of the most suitable expression platform for their favorite protein for technical or pharmaceutical purposes. In addition, the book is also a valuable study resource for professors and students in the fields of applied biology and biotechnology."
Download or read book Non conventional Yeasts from Basic Research to Application written by Andriy Sibirny and published by Springer. This book was released on 2019-08-12 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume scopes several aspects of non-conventional yeast research prepared by the leading specialists in the field. An introduction on taxonomy and systematics enhances the reader’s knowledge on yeasts beyond established ones such as Saccharomyces cerevisiae. Biotechnological approaches that involve fungal utilization of unusual substrates, production of biofuels and useful chemicals as citric acid, glutathione or erythritol are discussed. Further, strategies for metabolic engineering based on knowledge on regulation of gene expression as well as sensing and signaling pathways are presented. The book targets researchers and advanced students working in Microbiology, Microbial Biotechnology and Biochemistry.
Download or read book Yeast Biotechnology Diversity and Applications written by T. Satyanarayana and published by Springer Science & Business Media. This book was released on 2009-04-24 with total page 747 pages. Available in PDF, EPUB and Kindle. Book excerpt: I belie ve that the book would provide an overview of the recent developments in the domain of yeast research with some new ideas, which could serve as an inspiration and challenge for researchers in this field. Ne w Delhi Prof. Asis Datta Dec. 24, 2007 F ormer Vice-chancellor, JNU Director, NCPGR (New Delhi) Pr eface Yeasts are eukaryotic unicellular microfungi that are widely distributed in the natural environments. Although yeasts are not as ubiquitous as bacteria in the na- ral environments, they have been isolated from terrestrial, aquatic and atmospheric environments. Yeast communities have been found in association with plants, a- mals and insects. Several species of yeasts have also been isolated from specialized or extreme environments like those with low water potential (e. g. high sugar/salt concentrations), low temperature (e. g. yeasts isolated from Antarctica), and low oxygen availability (e. g. intestinal tracts of animals). Around 1500 species of yeasts belonging to over 100 genera have been described so far. It is estimated that only 1% of the extant yeasts on earth have been described till date. Therefore, global efforts are underway to recover new yeast species from a variety of normal and extreme environments. Yeasts play an important role in food chains, and carbon, nitrogen and sulphur cycles. Yeasts can be genetically manipulated by hybridization, mutation, rare m- ing, cytoduction, spheroplast fusion, single chromosomal transfer and transfor- tion using recombinant technology. Yeasts (e. g.
Download or read book Yeast Diversity in Human Welfare written by Tulasi Satyanarayana and published by Springer. This book was released on 2017-05-13 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book brings together and updates the latest information on the diversity of yeasts, their molecular features and their applications in the welfare of mankind. Yeasts are eukaryotic microfungi widely found in natural environments, including those with extreme conditions such as low temperatures, low oxygen levels and low water availability. To date, approximately 2,000 of the estimated 30,000 to 45,000 species of yeast on Earth, belonging to around 200 genera have been described. Although there are a few that are opportunistic human and animal pathogens, the vast majority of yeasts are beneficial, playing an important role in the food chain and in the carbon, nitrogen and sulphur cycles. In addition, yeasts such as Saccharomyces cerevisiae, Hansenula polymorpha and Pichia pastoris are used in expressing foreign genes to produce proteins of pharmaceutical interest. A landmark in biotechnology was reached in 1996 with the completion of sequencing of the entire S. cerevisiae genome, and it has now become a central player in the development of an entirely new approach to biological research and synthetic biology. The sequencing of genomes of several yeasts including Schizosaccharomyces pombe, Candida albicans and Cryptococcus neofromans has also recently been completed. candida albicans="" and="" p/pp
Download or read book Non Conventional Yeasts in Genetics Biochemistry and Biotechnology written by Klaus Wolf and published by Springer Science & Business Media. This book was released on 2003-03-04 with total page 538 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most information on yeasts derives from experiments with the conventional yeasts Saccaromyces cerevisiae and Schizossaccharomyces pombe, the complete nuclear and mitochondrial genome of which has also been sequenced. For all other non-conventional yeasts, investigations are in progress and the rapid development of molecular techniques has allowed an insight also into a variety of non-conventional yeasts. In this bench manual, over 70 practical protocols using 15 different non-conventional yeast species and in addition several protocols of general use are described in detail. All of these experiments on the genetics, biochemistry and biotechnology of yeasts have been contributed by renowned laboratories and have been reproduced many times. The reliable protocols are thus ideally suited also for undergraduate and graduate practical courses.
Download or read book Genetics and Biotechnology written by Ulrich Kück and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mycology, the study of fungi, originated as a subdiscipline of botany and was a descriptive discipline, largely neglected as an experimental science until the early years of this century. A seminal paper by Blakeslee in 1904 provided evidence for self incompatibility, termed "heterothallism", and stimulated interest in studies related to the control of sexual reproduction in fungi by mating-type specificities. Soon to follow was the demonstration that sexually reproducing fungi exhibit Mendelian inheritance and that it was possible to conduct formal genetic analysis with fungi. The names Burgeff, Kniep and Lindegren are all associated with this early period of fungal genetics research. These studies and the discovery of penicillin by Fleming, who shared a Nobel Prize in 1945, provided further impetus for experimental research with fungi. Thus began a period of interest in mutation induction and analysis of mutants for bio chemical traits. Such fundamental research, conducted largely with Neurospora crassa, led to the one gene: one enzyme hypothesis and to a second Nobel Prize for fungal research awarded to Beadle and Tatum in 1958. Fundamental research in biochemical genetics was extended to other fungi, especially to Saccharomyces cere visiae, and by the mid-1960s fungal systems were much favored for studies in eukaryotic molecular biology and were soon able to compete with bacterial systems in the molecular arena.
Download or read book The Yeasts written by Cletus Kurtzman and published by Elsevier. This book was released on 2011-05-09 with total page 2362 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Yeasts: A Taxonomic Study is a three-volume book that covers the taxonomic aspect of yeasts. The main goal of this book is to provide important information about the identification of yeasts. It also discusses the growth tests that can be used to identify different species of yeasts, and it examines how the more important species of yeasts provide information for the selection of species needed for biotechnology. • Volume 1 discusses the identification, classification and importance of yeasts in the field of biotechnology. • Volume 2 focuses on the identification and classification of ascomycetous yeasts. • Volume 3 deals with the identification and classification of basidiomycetous yeasts, along with the genus Prototheca. High-quality photomicrographs and line drawings Detailed phylogenetic trees Up-to-date, clearly presented yeast taxonomy and systematic, easy-to-use reference sequence accession numbers to allow for correct identification
Download or read book Recombinant Protein Production in Yeast written by Brigitte Gasser and published by Humana Press. This book was released on 2019-02-09 with total page 431 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an overview of the main yeast production platforms currently used and future yeast cell factories for recombinant protein production. Chapters detail approaches of genetic and metabolic engineering, co-factor containing proteins and virus-like particles, glycoproteins, and post-translational modifications of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Recombinant Protein Production in Yeast: Methods and Protocols aims to provide state of the art background and methods for protein producing yeast platforms, as well as case studies for special applications.
Download or read book Pichia Protocols written by James M Cregg and published by Springer Science & Business Media. This book was released on 2007-08-08 with total page 553 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.
Download or read book Nonconventional Yeasts in Biotechnology written by Klaus Wolf and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 631 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book to extensively and exclusively cover nonconventional yeasts - all yeasts other than S. cerevisiae and S. pombe. In addition to useful background information, the author includes detailed protocols allowing the investigation of basic and applied aspects for a wide range of these organisms. Due to the increasing importance of nonconventional yeasts in biotechnological applications, this book should become the standard reference for both pure and applied scientists working in the fields of microbiology and biochemistry.
Download or read book Fungi written by Kevin Kavanagh and published by John Wiley & Sons. This book was released on 2011-08-04 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fungi: Biology and Applications, Second Edition provides a comprehensive treatment of fungi, covering biochemistry, genetics and the medical and economic significance of these organisms at introductory level. With no prior knowledge of the subject assumed, the opening chapters offer a broad overview of the basics of fungal biology, in particular the physiology and genetics of fungi and also a new chapter on the application of genomics to fungi. Later chapters move on to include more detailed coverage of topics such as antibiotic and chemical commodities from fungi, new chapters on biotechnological use of fungal enzymes and fungal proteomics, and fungal diseases of humans, antifungal agents for use in human therapy and fungal pathogens of plants.
Download or read book Autophagy written by Daniel Klionsky and published by CRC Press. This book was released on 2003-12-15 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: Starting in the early 1970s, a type of programmed cell death called apoptosis began to receive attention. Over the next three decades, research in this area continued at an accelerated rate. In the early 1990s, a second type of programmed cell death, autophagy, came into focus. Autophagy has been studied in mammalian cells for many years. The recen
Download or read book Industrial Biotechnology written by Christoph Wittmann and published by John Wiley & Sons. This book was released on 2017-04-10 with total page 789 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest volume in the Advanced Biotechnology series provides an overview of the main production hosts and platform organisms used today as well as promising future cell factories in a two volume book. Alongside describing tools for genetic and metabolic engineering for strain improvement, the authors also impart topical information on computational tools, safety aspects and industrial-scale production. Following an introduction to general concepts, historical developments and future technologies, the text goes on to cover multi-purpose bacterial cell factories, including those organisms that exploit anaerobic biosynthetic power. Further chapters deal with microbes used for the production of high-value natural compounds and those obtained from alternative raw material sources, concluding with eukaryotic workhorses.
Download or read book Microbial Growth on C1 Compounds written by H.W. Verseveld and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: The 5th International Symposium on Microbial Growth on C Compounds was held at the Biological 1 Center of the University of Groningen, Haren, The Netherlands, 11-16 August 1986. The meeting attracted well over 200 participants from 15 countries. This volume contains the formal presentations made at that time, which, because of the breadth of topics covered, were divided into seven sections of related papers. This meeting, under the chairmanship of Wim Harder, was both scientifically and socially very successful. This success cannot only be credited to the main presentations, but also to the well cared for 121 poster presentations, whereof the abstracts have been published separately. The series of Symposia will be continued in 1989, in the Federal Republic of Germany. We wish to acknowledge the invaluable help of Joke Daniels, Roberta Stroer-Schneider, Karin Uyldert, Hansje Bartelson and Josine van Verseveld-Stroer, who retyped the manuscripts resulting in a uniform presentation of these proceedings.
