Download or read book The Genetic Analysis of an Insertion Mutation in Yeast written by Deborah Tardy Chaleff and published by . This book was released on 1981 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Research Awards Index written by and published by . This book was released on 1989 with total page 776 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Yeast Gene Analysis written by and published by Academic Press. This book was released on 1998-04-15 with total page 519 pages. Available in PDF, EPUB and Kindle. Book excerpt: Established almost 30 years ago, Methods in Microbiology is the most prestigious series devoted to techniques and methodology in the field. Now totally revamped, revitalized, with a new format and expanded scope, Methods in Microbiology will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research.Focuses on the methods most useful for the functional analysis of yeast genesAllows researcher to identify which strategy to use without having to wade through numerous recipesIncludes techniques for mutagenesis, transcript analysis, transposon tagging, and use of reporter genesDescribes the use of tools for studying post-translational modifications in yeastIncludes useful appendices with handy basic yeast recipes and WWW addresses
Download or read book Phenotypic and Genetic Analysis of D 2 hydroxyglutarate Overproducing Yeast Mutants written by Sophie Fiola and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "The vast majority of low-grade gliomas (LGGs) carry somatic mutations in isocitrate dehydrogenase 1 (IDH1) and 2 (IDH2) genes. IDH1 and IDH2 catalyze the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (alpha-KG) in an NADP+ dependent manner. A point mutation (R132H in IDH1 and R172H in IDH2) confers the neomorphic ability for the enzyme to reduce alpha-KG to D-2-hydroxyglutarate (D2HG). D2HG is converted to alpha-KG by the mitochondrial enzyme D-2HG dehydrogenase (D2HGDH). The accumulation of D2HG contributes to tumourigenesis through a variety of mechanisms including decreased utilization of oxidative phosphorylation and histone hypermethylation. The use of the budding yeast Saccharomyces cerevisiae as a model system to study cancer allows for faster, more efficient elucidation of various molecular mechanisms, including functional genomics via genetic array screening. In S. cerevisae, the NADP+ dependent isocitrate dehydrogenases are encoded by three different genes, IDP1, IDP2 and IDP3, and the homolog of D2HGDH is the mitochondrial DLD3 and the cytosolic DLD2. We have successfully generated a yeast model that carries the analogous mutation in the yeast IDP1 gene (idp1R148H). The allele was inserted at the HO locus, which does not alter the endogenous IDP1 gene. In this way, the resulting strain carries both a wild-type and mutant allele of IDP, more closely mimicking the metabolic state of glioma cells. We have validated this insertion by PCR, sequencing, and tetrad analysis. The production of the mutant Idp1R148H protein was detected by Western blot. This strain did not phenocopy the idp1∆ strain, suggesting the function of the endogenous IDP1 allele is unaffected. We have characterized the phenotype of both the idp1R148H and dld3∆ strains by growth profiling and analysis of chronological life span. The idp1R148H strain shows normal growth on glucose-containing media and a normal chronological lifespan. The dld3∆ strain shows impaired growth on glucose-containing media and a decreased chronological lifespan. Incubation of a wild-type strain of yeast with a membrane-permeable octyl D2HG revealed a dose-dependent growth impairment, which was persistent at higher concentrations. In addition, neither the dld3∆ nor the idp1R148H strain was sensitive to DNA damage by the alkylating agent MMS. Using publicly available synthetic genetic array analysis data from TheCellMap.org, we investigated the negative genetic interactions with DLD3. Analysis of the DLD3 interactions suggested that the biosynthesis of mitochondrial membrane phospholipids could be dysregulated in D2HG-overexpressing cells. Taken together, these data represent the first step in the characterization of D2HG-overproducing strains of yeast. These models will be useful in elucidating the molecular changes by which D2HG contributes to gliomagenesis"--
Download or read book Guide to Yeast Genetics Functional Genomics Proteomics and Other Systems Analysis written by and published by Academic Press. This book was released on 2010-02-27 with total page 961 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully updated edition of the bestselling three-part Methods in Enzymology series, Guide to Yeast Genetics and Molecular Cell Biology is specifically designed to meet the needs of graduate students, postdoctoral students, and researchers by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. This volume serves as an essential reference for any beginning or experienced researcher in the field. - Provides up-to-date methods necessary to study genes in yeast - Includes proceedures that enable newcomers to set up a yeast laboratory and to master basic manipulations - Serves as an essential reference for any beginning or experienced researcher in the field
Download or read book Yeast Genetics written by John F.T. Spencer and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt: The manual consists of two main sections. The first includes the essential, sometimes laborious, procedures for handling yeasts, for inducing mating and isolation of hybrids, for inducing sporulation and isolation of single-spore clones, with some details of tetrad analysis, and including techniques and ancillary equipment for use of the micromanipulator. There are also proce dures for induction of mutants by physical and chemical agents, and for iso lation of particular types of mutants, such as to temperature sensitivity, for increased frequency of mutations, for mutations in the mitochondrial genome, both to the petite colonie form and to resistance to antibiotics, for mutations in that part of the yeast genome controlling the glycolytic cycle, and numerous others. Mapping of mutations is discussed briefly, though this aspect of yeast genetics is probably one which should not be undertaken until the investigator has gained a certain amount of experience in the field. How ever, as is pointed out in the pertinent part of the manual, the task of mapping has been tremendouslysimplified by the availability from the Yeast Genetics Stock Center at the University of California at Berkeley of a set of auxo trophic strains designed to permit mapping of most unknown genes with a minimum number of crosses and tetrad analyses. The first section concludes with the description of methods for hybridization of yeasts by protoplast fusion, which has been described as the poor man's system for genetic engineering.
Download or read book Genetic Analysis of Mutations Affecting the Initiation of Yeast Sporulation written by Anne Donaldson Ramage and published by . This book was released on 1990 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomedical Index to PHS supported Research pt A Subject access A H written by and published by . This book was released on 1992 with total page 1064 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book An Introduction to Genetic Analysis written by Anthony J.F. Griffiths and published by Macmillan. This book was released on 2005 with total page 808 pages. Available in PDF, EPUB and Kindle. Book excerpt: The eighth edition of 'An Introduction to Genetic Analysis' has been extensively revised, shaping its coverage to match current research and thinking in genetics.
Download or read book Genetic Analysis written by Philip Mark Meneely and published by Oxford University Press. This book was released on 2014 with total page 580 pages. Available in PDF, EPUB and Kindle. Book excerpt: With its unique integration of genetics and molecular biology, this text probes fascinating questions that explore how our understanding of key genetic phenomena can be used to understand biological systems. Opening with a brief overview of key genetic principles, model organisms, and epigenetics, the book goes on to explore the use of gene mutations, the analysis of gene expression and activity, a discussion of the genetic structure of natural populations, and more.
Download or read book Recent Developments in Yeast Genetics written by Seymour Pomper and published by . This book was released on 1950 with total page 24 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Genetic Manipulation of DNA and Protein written by David Figurski and published by IntechOpen. This book was released on 2013-02-05 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This diverse collection of research articles is united by the enormous power of modern molecular genetics. Every author accomplished two objectives: (1) making the field and the research described accessible to a large audience and (2) explaining fully the genetic tools and approaches that were used in the research. One fact stands out - the importance of a genetic approach to addressing a problem. I encourage you to read several chapters. You will feel the excitement of the scientists, and you will learn about an area of research with which you may not be familiar. Perhaps most importantly, you will understand the genetic approaches; and you will appreciate their importance to the research.
Download or read book Galactose Regulon of Yeast written by Paike Jayadeva Bhat and published by Springer Science & Business Media. This book was released on 2008-03-02 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: This self contained book presents a comprehensive overview of the past, present and future of the galactose regulon of yeast, the classical model system of molecular biologists. The book starts with a brief historical overview on yeast research. This is followed by molecular genetics of the galactose regulon, isolation of genes and testing of the hypotheses. Contemporary topics including genomics, evolution, binary and graded responses, and stochasticity are all addressed.
Download or read book Genetic Analysis of Mutations Affecting the Initiation of Yeast Sporulation written by Anne Donaldson Ramage and published by . This book was released on 1990 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biochemical Analysis Tools written by Oana-Maria Boldura and published by BoD – Books on Demand. This book was released on 2020-06-24 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book explores the role of nucleic acid analysis and the advances it has led to in the field of life sciences. The first section is a collection of chapters covering experimental methods used in molecular biology, the techniques adjacent to these methods, and the steps of analysis before and after obtaining raw DNA data. The second section deals with the principles of chromatography, method development, sample preparation, and industrial applications.
Download or read book Dissertation Abstracts International written by and published by . This book was released on 1995 with total page 876 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Biomedical Index to PHS supported Research written by and published by . This book was released on 1987 with total page 1398 pages. Available in PDF, EPUB and Kindle. Book excerpt:
