Download or read book The Defense Response of Glycine Max to Its Major Parasitic Nematode Pathogen Heterodera Glycines written by Shankar R. Pant and published by . This book was released on 2016 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: Heterodera glycines, soybean cyst nematode (SCN) causes more than one billion dollars soyben production loss in the U.S. annually. SCN is an obligate parasite of specialized feeding cells within the host root known as syncytium. The SCN resistance genes and signaling pathways in soybean have not been fully characterized. Gene expression analysis in syncytium from compatible and incompatible interactions identified candidate genes that might involve conferring resistance to SCN. This dissertation aimed to investigate the biological functions of the candidate resistance genes to confirm the roles of these genes in resistance to SCN. The study demonstrated a role of syntaxin 31-like genes (Gm-SYP38) in resistance to SCN. Overexpression of Gm-SYP38 induced the transcriptional activity of the cytoplasmic receptor-like kinase BOTRYTIS INDUCED KINASE 1 (Gm-BIK1-6). Overexpression of Gm-BIK1-6 rescued the resistant phenotype. In contrast, Gm-BIK1-6 RNAi increased parasitism. In another experiment, the expression of a Glycine max homolog of LESION SIMULATING DISEASE1 (LSD1) resulted in the transcriptional activation of ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1) and NONEXPRESSOR OF PR1 (NPR1), that function in salicylic acid (SA) signaling, implicating the involvement of the antiapoptotic, environmental response gene LESION SIMULATING DISEASE1 (LSD1) in defense that is demonstrated here. The study also investigated the role of SNARE components (genes functioning in membrane fusion) in resistance to SCN. Experiments showed that SNARE functions in concert with a beta-glucosidase having homology to PEN2 and an ATP binding cassette transporter having homology to PEN3. This study provides novel information for the genetic improvement of soybean for enhanced disease resistance.
Download or read book The Defense Response of I Glycine Max I to Its Major Parasitic Nematode Pathogen I Heterodera Glycines I written by and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Heterodera glycines , soybean cyst nematode (SCN) causes more than one billion dollars soyben production loss in the U.S. annually. SCN is an obligate parasite of specialized feeding cells within the host root known as syncytium. The SCN resistance genes and signaling pathways in soybean have not been fully characterized. Gene expression analysis in syncytium from compatible and incompatible interactions identified candidate genes that might involve conferring resistance to SCN. This dissertation aimed to investigate the biological functions of the candidate resistance genes to confirm the roles of these genes in resistance to SCN. The study demonstrated a role of syntaxin 31-like genes (Gm-SYP38) in resistance to SCN. Overexpression of Gm-SYP38 induced the transcriptional activity of the cytoplasmic receptor-like kinase BOTRYTIS INDUCED KINASE 1 (Gm-BIK1-6). Overexpression of Gm-BIK1-6 rescued the resistant phenotype. In contrast, Gm-BIK1-6 RNAi increased parasitism. In another experiment, the expression of a Glycine max homolog of LESION SIMULATING DISEASE1 (LSD1) resulted in the transcriptional activation of ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1) and NONEXPRESSOR OF PR1 (NPR1), that function in salicylic acid (SA) signaling, implicating the involvement of the antiapoptotic, environmental response gene LESION SIMULATING DISEASE1 (LSD1) in defense that is demonstrated here. The study also investigated the role of SNARE components (genes functioning in membrane fusion) in resistance to SCN. Experiments showed that SNARE functions in concert with a beta-glucosidase having homology to PEN2 and an ATP binding cassette transporter having homology to PEN3 . This study provides novel information for the genetic improvement of soybean for enhanced disease resistance.
Download or read book An Analysis of Signaling Processes Leading to a Defense Response in Soybean written by and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant-parasitic nematodes are the cause of devastating yield loss in vital agricultural crops around the world. Heterodera glycines , also referred to as soybean cyst nematode, is the main pathogen of Glycine max (soybean) causing more loss than all other pathogens of G. max combined. The resultant economic impact due to H. glycines in United States soybean production alone is estimated to account for an annual one-billion-dollar loss. Natural resistant genotypes have been found in trials to combat this pathogen. Of the resistant varieties identified, G. max [Peking/PI 548402] and G. max [PI 88788] are the major sources of resistance. Identification of genes expressed in the cells of which the nematode parasitizes, the syncytia, exclusively undergoing the resistant/incompatible reaction from the two major sources of resistance mentioned previously have identified a number of candidate genes presumed to function in defense to H. glycines parasitism. Prior to this work, success has been obtained by selection of a number of these candidate genes in functional analysis to show involvement in defense. This work is aimed at functionally identifying signaling components involved in the defense reaction. Reverse genetic studies of NON-RACE SPECIFIC DISEASE RESISTANCE 1 Glycine max homolog, Gm-NDR1-1, has confirmed a functional role in the defense to H. glycines to G. max. Gene expression studies revealed both effector-triggered immunity (ETI) and pattern-triggered immunity (PTI) components to be regulated by Gm-NDR1-1. Furthermore, induction in the heterologous expression of Gm-NDR1-1 in Gossypium hirsutum (cotton) suppressed Meloidogyne incognita parasitism. Harpin treatment has been evaluated due to the knowledge of NDR1s capability of being harpin-induced (HIN1). Expression studies of the harpin treatment did in fact induce Gm-NDR1-1. The analysis further provides ev
Download or read book A Functional Analysis of the Defense Response of Glycine Max as it Relates to Parasitism by the Plant Parasitic Nematode Heterodera Glycines written by Prakash Mani Niraula and published by . This book was released on 2019 with total page 159 pages. Available in PDF, EPUB and Kindle. Book excerpt: The soybean cyst nematode (SCN), Heterodera glycines, a plant parasitic pest, causes severe yield losses of soybean (Glycine max). Although a number of studies have identified various genes that function in defense, including a role for the vesicular transport machinery acting against H. glycines in infected roots, a regulatory mechanism occurring behind the transcriptional engagement of the vesicular transport system and delivery of the transported cargo proteins is not fully understood. The main goal of the current study is to determine the functional effect of genetically engineering the circadian clock gene, CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) in G. max to examine a role on H. glycines parasitism. The outcome of the study has determined the functional effect of main clock component CCA1-1 along with other oscillator genes such as TIMING OF CAB 1 (TOC1-1), GIGANTEA (GI-1) and CONSTANS (CO-4) to enhance resistance against H. glycines parasitism. Further, the reduced level of the expression of Gm-CCA1-1 in infected roots, in comparison to uninfected roots, has demonstrated that clock components might have arrested and altered its expression during the nematode infection process. The study has also investigated the role of XYLOGLUCAN ENDO-TRANSGLYCOSYLASE /HYDROLASE (XTH), Gm-XTH43, during the resistance process soybean has to H. glycines. The results have demonstrated higher xyloglucan (XyG) amounts to be synthesized in the Gm-XTH43 overexpressing (OE) lines. In contrast, there is less XyG in the Gm-XTH43 RNA interference (RNAi) lines that have a negatively regulated XTH gene. These observations have led to elucidating the role in the potential cell wall rearrangement and the underlying metabolic processes required for the generation of the proper XyG architecture required for defense occurring outside of the plant cell. Furthermore, the observed result of lower level of weight average molecular weight (WAMW) of XyG in Gm-XTH43-OE and higher MW of XyG in Gm-XTH43-RNAi than respective control roots have demonstrated a key role in, presumably, changing the cell wall by the remodeling of the XyG chain as it relates to the cell wall architecture.
Download or read book An Analysis of Signaling Processes Leading to a Defense Response in Soybean written by Brant Trey McNeece and published by . This book was released on 2017 with total page 225 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant-parasitic nematodes are the cause of devastating yield loss in vital agricultural crops around the world. Heterodera glycines, also referred to as soybean cyst nematode, is the main pathogen of Glycine max (soybean) causing more loss than all other pathogens of G. max combined. The resultant economic impact due to H. glycines in United States soybean production alone is estimated to account for an annual one-billion-dollar loss. Natural resistant genotypes have been found in trials to combat this pathogen. Of the resistant varieties identified, G. max[Peking/PI 548402] and G. max[PI 88788] are the major sources of resistance. Identification of genes expressed in the cells of which the nematode parasitizes, the syncytia, exclusively undergoing the resistant/incompatible reaction from the two major sources of resistance mentioned previously have identified a number of candidate genes presumed to function in defense to H. glycines parasitism. Prior to this work, success has been obtained by selection of a number of these candidate genes in functional analysis to show involvement in defense. This work is aimed at functionally identifying signaling components involved in the defense reaction. Reverse genetic studies of NON-RACE SPECIFIC DISEASE RESISTANCE 1 Glycine max homolog, Gm-NDR1-1, has confirmed a functional role in the defense to H. glycines to G. max. Gene expression studies revealed both effectortriggered immunity (ETI) and pattern-triggered immunity (PTI) components to be regulated by Gm-NDR1-1. Furthermore, induction in the heterologous expression of GmNDR1-1 in Gossypium hirsutum (cotton) suppressed Meloidogyne incognita parasitism. Harpin treatment has been evaluated due to the knowledge of NDR1’s capability of being harpin-induced (HIN1). Expression studies of the harpin treatment did in fact induce GmNDR1-1. The analysis further provides evidence of NDR1 role in defense by displaying the harpin-induced response of NDR1 in resistance to infection of Rotylenchulus reniformis. Receptors are known to function through signaling components in plant defense. Therefore, the conserved downstream signaling component of multiple diverse stimuli, mitogen-activated protein kinases (MAPKs) were functionally characterized in G. max for their role in resistance to H. glycines via the reverse genetic parasitism assays and evaluated to observe the effect on defense gene expression.
Download or read book Investigating Root knot and Soybean Cyst Nematode Parasitic Interactions Through Transcriptomic Analyses of the Host and Parasite written by Ellie Walsh and published by . This book was released on 2016 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant-parasitic nematodes are a major threat to global agricultural production. Root-knot nematodes (RKN, Meloidogyne spp.) are arguably the biggest threat, capable of parasitizing virtually every crop. Soybean cyst nematode (SCN; Heterodera glycines) has a narrow host range, but is the most destructive pathogen of a particularly important crop, soybean (Glycine max). RKN’s wide host range makes crop rotation often inadequate for management, and host resistance is unavailable in many crops. Effective resistance is available against SCN however populations have adapted to the most frequently used sources of resistance. RKN and SCN both induce elaborate feeding sites. In addition to being the sole source of nutrition, the feeding sites are the primary targets of nematode secretions to manipulate host cellular functions; consequently, they are very important interfaces of the interaction. The general aim of this research was to elucidate changes in the transcriptome underlying the successful interaction between these nematodes and their hosts. Although the use of RNA interference (RNAi) to knockdown nematode genes is actively being pursued as a new strategy for nematode control, little is known about the effects of general RNAi mechanisms during parasitism. As the suppression of RNAi has been characterized in other pathosystems, I hypothesized that parasitic nematodes may also be influencing these pathways. Tanscriptomic analysis of genes associated with RNAi machinery and target genes indicates that RNAi-regulated pathways are altered during the parasitic interaction. Using a silenced reporter gene, I found the disturbance to be specific to the nematode feeding site. Furthermore, disrupting these pathways with viral suppressors of RNAi renders the host more susceptible to nematode parasitism. Transcriptomic analysis indicates that this effect extends into later stages in parasitism, making the adult female stage of particular interest for further analyses. I performed a transcriptomic analysis of adult female RKN to address the hypothesis that transcriptional patterns in this later stage of parasitism will reveal new candidate genes encoding proteins that regulate the parasitic process, such as proteins that interact with RNAi among other plant pathways. Results from RNA-Seq analysis and reverse transcriptase PCR indicate that cell wall modifiers likely continue to play an important role in the parasitic interaction. Results from transcriptomic analysis including the putative secretome have highlighted new candidates for functional analysis to determine their role in the interaction. The later stage in parasitism is similarly of interest in SCN. SCN populations are adapting to the most commonly planted host resistance available in soybean, derived from Plant Introduction (PI) 88788 and “Peking.” The resistance response in PI 88788 appears to be longer-lasting than that in Peking, which impacts nematodes’ early development. Due to my focus on the adult female stage, I chose to investigate parasitism on PI 88788. I hypothesize that transcriptional differences between females from populations avirulent and virulent on PI 88788 may play a role in their adaptation to resistance. Results from this study indicate that the expression of an effector-like gene may have been lost in virulent populations, presumably allowing them to evade host detection and subsequent defense responses.
Download or read book Plant Nematode Interactions written by and published by Academic Press. This book was released on 2015-03-26 with total page 461 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Botanical Research publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. Currently in its 73rd volume, the series features several reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. This thematic volume features reviews on molecular and developmental aspects of the compatible plant-nematode interaction. The contributors all actively work in the field of molecular genetics and genomics of plant parasitic nematodes and nematode feeding sites. Reviews focus on molecular and physiological aspects of nematode feeding site development and includes specific chapters on nematode effectors as well as plant responses. Publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences This volume features reviews of the fast moving field of compatible interaction between plants and sedentary endo-parasitic nematodes A strong focus on molecular and physiological aspects of nematode feeding site development and includes specific chapters on nematode effectors as well as plant responses
Download or read book Genomics and Molecular Genetics of Plant Nematode Interactions written by John Jones and published by Springer Science & Business Media. This book was released on 2011-04-26 with total page 563 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book reviews developments in the molecular biology of plant-nematode interactions that have been driven by the application of genomics tools. The book will be of interest to postgraduate students and to researchers with an interest in plant nematology and/or plant pathology more generally. A series of introductory chapters provide a biological context for the detailed reviews of all areas of plant-nematode interactions that follow and ensure that the bulk of the book is accessible to the non-specialist. A final section aims to show how these fundamental studies have provided outputs of practical relevance.
Download or read book Genetic Maps written by Stephen J. O'Brien and published by . This book was released on 1990 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Protecting Our Crops Approaches for Plant Parasitic Nematode Control written by Claudia S. L. Vicente and published by Frontiers Media SA. This book was released on 2021-11-15 with total page 307 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Physiology and Biochemistry of Free living and Plant parasitic Nematodes written by Roland N. Perry and published by Cabi. This book was released on 1998 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Written by leading research workers from Europe, USA, Australia and New Zealand, this is the only up-to-date reference book which reviews and integrates all the current research findings on the physiology and biochemistry of these organisms, including the molecular information which has accumulated in recent years.
Download or read book Cellular and Molecular Aspects of Plant Nematode Interactions written by Carmen Fenoll and published by Springer Science & Business Media. This book was released on 1997 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 1992 a Concerted Action Programme (CAP) was initiated by Peter Sijmons with the purpose of intensifying collaborations between 16 European laboratories working on plant-parasitic nematodes. The four-year programme entitled Resistance Mechanisms Against Plant-Parasitic Nematodes focused on molecular aspects of the interaction between sedentary nematodes and plants on the model system Arabidopsis and on novel resistance strategies. Funding was provided mainly for exchange visits between collaborating laboratories and for the organization of annual meetings. During the last annual meeting which was held in May 1996 in Toledo, Spain, Carmen Fenoll initiated the production of this volume.
Download or read book Plant Nematology 3rd Edition written by Roland N. Perry and published by CABI. This book was released on 2024-07-12 with total page 661 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant-parasitic nematodes are a major threat to crop production in all countries, and identification of nematodes and knowledge of their biology is of paramount importance. Providing a broad introduction to nematodes as plant parasites, this book begins by describing nematodes by genera, and then builds on this foundation to detail nematode biology and pest management, including resistance, and biological and chemical control. This 3rd edition is updated to address advances in our knowledge of plant-parasitic nematodes, particularly concerning the molecular aspects of host-parasite relationships, including the application of genomics to nematode biology. Changes in control and management strategies are also covered. The influence of climate change on plant-parasitic nematodes is addressed in a new chapter on ecology. Primarily aimed at students (MSc and PhD), this book is also invaluable for researchers and lecturers in nematology, plant pathology and parasitology, advisory and quarantine services, and crop protection scientists.
Download or read book Managing Cover Crops Profitably 3rd Ed written by Andy Clark and published by DIANE Publishing. This book was released on 2008-07 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cover crops slow erosion, improve soil, smother weeds, enhance nutrient and moisture availability, help control many pests and bring a host of other benefits to your farm. At the same time, they can reduce costs, increase profits and even create new sources of income. You¿ll reap dividends on your cover crop investments for years, since their benefits accumulate over the long term. This book will help you find which ones are right for you. Captures farmer and other research results from the past ten years. The authors verified the info. from the 2nd ed., added new results and updated farmer profiles and research data, and added 2 chap. Includes maps and charts, detailed narratives about individual cover crop species, and chap. about aspects of cover cropping.
Download or read book Soybean Breeding written by Felipe Lopes da Silva and published by Springer. This book was released on 2017-06-10 with total page 439 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book was written by soybean experts to cluster in a single publication the most relevant and modern topics in soybean breeding. It is geared mainly to students and soybean breeders around the world. It is unique since it presents the challenges and opportunities faced by soybean breeders outside the temperate world.
Download or read book Plant Parasitic Nematodes written by Bert Zuckerman and published by Elsevier. This book was released on 2012-12-02 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Parasitic Nematodes, Volume III provides a comprehensive discussion of the different advances in plant nematology. This includes biochemical techniques to taxonomy and innovation in transmission and scanning electron microscopy technology. It explains a broadened basis for understanding nematode physiology and behavior and the sensory mechanisms that govern nematode actions and plant host-nematode interactions. The book discusses the development of modern approaches to the evaluation and reduction of crop losses. The emphasis of this volume is on plant parasites and insights gained through research on other nematodes. In particular, the book explains the anatomical, developmental, behavioral, and genetic studies on the free-living nematode Cenorhabditis elegans, which is a widely used laboratory model for examining various biological problems. The information provided by various researches on C. elegans increases our understanding about the relevance of nematodes to general biological processes in higher organisms, including man. The book is divided into 19 chapters which cover the following concepts of plant nematology: biochemistry, cytochemistry, and genetics; morphology and function; host-parasite relations; and evaluation and control of crop losses. The present volume is an excellent reference for students, lecturers, and research professionals in plant parasitology and related fields.
Download or read book Control of Plant parasitic Nematodes written by and published by National Academies. This book was released on 1968 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt:
