Download or read book Techniques for Plant Electron Microscopy written by Barrie Edward Juniper and published by Blackwell Scientific Publications Limited. This book was released on 1970 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fixation. Embedding. Microtomy. General stains. Cytochemical stains. Negative staining. Specimen support films. Replicas. Preparation for scanning electron microscopy. Special techniques. Autoradiography. The instrument and photography.
Download or read book Methods in Plant Electron Microscopy and Cytochemistry written by William V. Dashek and published by Springer Science & Business Media. This book was released on 2000-06-29 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on experimentalists describe the cutting-edge microscopical methods needed for the effective study of plant cell biology today. These powerful techniques, all described in great detail to ensure successful experimental results, range from light microscope cytochemistry, autoradiography, and immunocytochemistry, to recent developments in fluorescence, confocal, and dark-field microscopies. Important advances in both conventional and scanning electron microscopies are also fully developed, together with such state-of-the-art ancillary techniques as high-resolution autoradiography, immunoelectron microscopy, X-ray microanalysis, and electron systems imaging. Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.
Download or read book Techniques for Plant Electron Microscopy written by B. E. Juniper and published by . This book was released on 1970 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electron Microscopy of Plant Cells written by C Hawes and published by Academic Press. This book was released on 2012-12-02 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.
Download or read book Electron Microscopy of Plant Pathogens written by Kurt Mendgen and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.
Download or read book Basic Techniques For Transmission Electron Microscopy written by M.A. (Eric) Hayat and published by Elsevier. This book was released on 2012-12-02 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Techniques for Transmission Electron Microscopy describes the basic techniques for transmission electron microscopy. Preparatory procedures for both eukaryotic and prokaryotic groups are presented in a step-by-step fashion, together with special preparatory methods for plant specimens and viruses. The processing of uncommon specimens and the solution of unusual, individual problems are included. This book is comprised of seven chapters and begins with a discussion on chemical fixation, with particular reference to fixatives and the hazards, precautions, and safe handling of reagents, as well as the preparation of buffers and tissue blocks. The reader is then introduced to the standard procedure for fixation, rinsing, dehydration, and embedding. Subsequent chapters focus on sectioning, cryofixation, and cryoultramicrotomy; positive and negative staining; and the use of support films. The final chapter presents a wide variety of specimens such as algae, amoeba, anthers, actin filaments, bacteria, and cells in culture. This monograph is essentially a laboratory handbook intended for students, technicians, teachers, and research scientists in biology and medicine.
Download or read book A Manual of Applied Techniques for Biological Electron Microscopy written by Michael J. Dykstra and published by Springer Science & Business Media. This book was released on 1993-08-31 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: This easy-to-follow manual describes tested procedures used to prepare biological samples for scanning and transmission electron microscopy, as well as methods for cytochemistry, immunocytochemistry, and scientific photography. The work is structured to clearly define testing objectives, necessary materials, procedural steps, and expected results; a list of references and trouble shooting techniques round out the text.
Download or read book Plant Cell Tissue and Organ Culture written by Oluf Gamborg and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.
Download or read book Sample Preparation Handbook for Transmission Electron Microscopy written by Jeanne Ayache and published by Springer Science & Business Media. This book was released on 2010-07-03 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti?c literature detailing speci?c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin spe- men preparation have appeared until this present work, ?rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.
Download or read book Techniques for Plant Electron Microscopy by B E Juniper and Others written by and published by . This book was released on 1970 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Electron Microscopy written by John Kuo and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the newest technology in electron microscopy. It comprises two major areas of electron microscopy - transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The volume provides clear, concise instructions on processing biological specimens and includes discussion on the underlying principles of the majority of the processes presented. A notes section enables efficient adaptation and troubleshooting of protocols.
Download or read book Electron Microscopy written by John J. Bozzola and published by Jones & Bartlett Learning. This book was released on 1999 with total page 702 pages. Available in PDF, EPUB and Kindle. Book excerpt: New edition of an introductory reference that covers all of the important aspects of electron microscopy from a biological perspective, including theory of scanning and transmission; specimen preparation; darkroom, digital imaging, and image analysis; laboratory safety; interpretation of images; and an atlas of ultrastructure. Generously illustrated with bandw line drawings and photographs. Annotation copyrighted by Book News, Inc., Portland, OR
Download or read book Principles and Techniques of Electron Microscopy written by M. A. Hayat and published by John Wiley & Sons. This book was released on 1970 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantitative mapping with the electron microscope; Photographic aspects of electron microscopy; Environmental devices in electron microscopy; Optical diffractometry; The analytical electron microscope, emma-4.
Download or read book Plant Techniques written by S.M. Khasim and published by CRC Press. This book was released on 2024-08-09 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book deals with the basic concepts of Plant Science including botanical micro technique and microtomy, staining techniques, molecular techniques, plant tissue culture, electron microscopy, and cryopreservation and germplasm storage. It is the outcome of several decades of research and teaching in plant biology to undergraduate and postgraduate students of Plant Science, Horticulture, Microbiology, and Biotechnology. Print edition not for sale in Bangladesh, Bhutan, India, Nepal, Pakistan, and Sri Lanka.
Download or read book Scanning Electron Microscopy in Taxonomy and Functional Morphology written by D. Claugher and published by Oxford University Press, USA. This book was released on 1990 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a convenient review of the latest developments in the use of the scanning electron microscope in the classification of plants and animals. It provides coverage of advances in equipment and preparative techniques, including the use of field emission, the viewing of uncoated materials, and image digitization.
Download or read book Principles and Techniques of Scanning Electron Microscopy written by M. A. Hayat and published by . This book was released on 1974 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Sample Preparation for Scanning Electron Microscopy and X Ray Microanalysis written by Patrick Echlin and published by Springer Science & Business Media. This book was released on 2011-04-14 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scanning electr on microscopy (SEM) and x-ray microanalysis can produce magnified images and in situ chemical information from virtually any type of specimen. The two instruments generally operate in a high vacuum and a very dry environment in order to produce the high energy beam of electrons needed for imaging and analysis. With a few notable exceptions, most specimens destined for study in the SEM are poor conductors and composed of beam sensitive light elements containing variable amounts of water. In the SEM, the imaging system depends on the specimen being sufficiently electrically conductive to ensure that the bulk of the incoming electrons go to ground. The formation of the image depends on collecting the different signals that are scattered as a consequence of the high energy beam interacting with the sample. Backscattered electrons and secondary electrons are generated within the primary beam-sample interactive volume and are the two principal signals used to form images. The backscattered electron coefficient ( ? ) increases with increasing atomic number of the specimen, whereas the secondary electron coefficient ( ? ) is relatively insensitive to atomic number. This fundamental diff- ence in the two signals can have an important effect on the way samples may need to be prepared. The analytical system depends on collecting the x-ray photons that are generated within the sample as a consequence of interaction with the same high energy beam of primary electrons used to produce images.
