Download or read book Surface Functionalization for Enhanced Fluorescence Detection Surface Plasmon Resonance Imaging and Microscopy written by Jennifer Betsy Fasoli and published by . This book was released on 2015 with total page 67 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work presents several high throughput imaging and analysis techniques performed by fluorescence detection and surface plasmon resonance biosensing. The microarray fabrication methods introduced in this thesis, as well as the DNA functionalization on planar and nanoparticle surfaces, enable and facilitate the real-time study of adsorption events via DNA- DNA hybridization and protein-DNA interaction. Silica deposited on polyolefin film serves as the base for the development of DNA microarray. Fabrication process involves poly(L-glutamic acid) monolayer to react with amine functional groups functionalized on both the silica surface and the probe DNA strands. Covalently surface-bound probes then hybridize with fluorophore-labeled target DNA. Shrinking the microarray with heat significantly enhances the sensitivity of detection via fluorescence signal amplification. Polydopamine, a biomimetic polymer, easily and rapidly grows an adhesive layer to link amine-terminated, single-stranded DNA to gold surface. Self-polymerization of polydopamine simply requires mild reagents and reaction condition. The microarray formed with polydopamine attachment technique reserves its stability and specificity to detect DNA and protein targets by surface plasmon resonance imaging. This one-step microarray fabrication approach, when coupled with signal amplification from DNA-modified gold nanoparticles, empowers the detection limit capable of seeing down to 10 pM target concentration. Electrochemical deposition of polydopamine demonstrates strong control over film localization and thickness. This meticulous polymerization on gold surface at the microscale proves the high toposelectivity of this deposition technique. Amine-modified DNA bound on this polydopamine thin flim hybridizes with fluorophore-tagged DNA to generate fluorescent DNA micropatterns. This electordeposition also forms DNA microarrays used in surface plasmon resonance imaging for DNA-DNA hybridization and DNA-coated gold nanoparticle adsorption measurements. A 814 nm, near-infrared surface plasmon resonance microscope exemplifies a novel, objective-coupled sensing and imaging system at single-nanoparticle scale. This microscope visualizes individual binding occurrence of gold nanoparticles and polystyrene beads. Adsorption analysis presents the calculated surface plasmon resonance signal ([delta]%R) as it varies with particle size. For initial biosensing measurements, the surface plasmon resonance microscopy examines each hybridization-adsorption of DNA-decorated gold nanoparticle on gold surface functionalized with either completely or partially complementary DNA monolayer.

Download or read book Surface Plasmon Enhanced Coupled and Controlled Fluorescence written by Chris D. Geddes and published by John Wiley & Sons. This book was released on 2017-03-03 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: Explains the principles and current thinking behind plasmon enhanced Fluorescence Describes the current developments in Surface Plasmon Enhanced, Coupled and Controlled Fluorescence Details methods used to understand solar energy conversion, detect and quantify DNA more quickly and accurately, and enhance the timeliness and accuracy of digital immunoassays Contains contributions by the world’s leading scientists in the area of fluorescence and plasmonics Describes detailed experimental procedures for developing both surfaces and nanoparticles for applications in metal-enhanced fluorescence

Download or read book Imaging of Surfaces by Surface Plasmon Resonance and Surface Plasmon Resonance enhanced Fluorescence written by Rahber A. Thariani and published by . This book was released on 2009 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Surface Plasmon Resonance in Bioanalysis written by and published by Elsevier. This book was released on 2021-11-11 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: Surface Plasmon Resonance in Bioanalysis, Volume 95 in the Comprehensive Analytical Chemistry series, contains a wide range of topics on the applications and new advances of surface plasmon resonance (SPR) in bioanalysis, including Surface plasmon resonance microscopy for single-cell based drug screening, Phase-Sensitive Surface Plasmon Resonance Sensors for Highly Sensitive Bioanalysis, SPR coupled to ambient mass spectrometry, Surface Plasmon Resonance Microscopy for activity detection and imaging of single cells, SPR for water pollutant detection and biofouling control, SPR imaging for cellular analysis and detection, Progress in detection of surface palsmon resonance for biorefinery technology, and more. Additional chapters cover Long-range surface plasmon resonance and its biological sensing applications and Critical issues in clinical and biomedical applications of Surface Plasmon Resonance sensing. Provides updates on the latest applications of SPR microscopy in cell analysis Covers the latest design in SPR sensing for highly sensitive bioanalysis Presents the critical issues in clinical and biomedical applications of SPR

Download or read book Metal Enhanced Fluorescence written by Chris D. Geddes and published by John Wiley & Sons. This book was released on 2010-06-22 with total page 655 pages. Available in PDF, EPUB and Kindle. Book excerpt: Discover how metal-enhanced fluorescence is changing traditional concepts of fluorescence This book collects and analyzes all the current trends, opinions, and emerging hot topics in the field of metal-enhanced fluorescence (MEF). Readers learn how this emerging technology enhances the utility of current fluorescence-based approaches. For example, MEF can be used to better detect and track specific molecules that may be present in very low quantities in either clinical samples or biological systems. Author Chris Geddes, a noted pioneer in the field, not only explains the fundamentals of metal-enhanced fluorescence, but also the significance of all the most recent findings and models in the field. Metal-enhanced fluorescence refers to the use of metal colloids and nanoscale metallic particles in fluorescence systems. It offers researchers the opportunity to modify the basic properties of fluorophores in both near- and far-field fluorescence formats. Benefits of metal-enhanced fluorescence compared to traditional fluorescence include: Increased efficiency of fluorescence emission Increased detection sensitivity Protect against fluorophore photobleaching Applicability to almost any molecule, including both intrinsic and extrinsic chromophores Following a discussion of the principles and fundamentals, the author examines the process and applications of metal-enhanced fluorescence. Throughout the book, references lead to the primary literature, facilitating in-depth investigations into particular topics. Guiding readers from the basics to state-of-the-technology applications, this book is recommended for all chemists, physicists, and biomedical engineers working in the field of fluorescence.

Download or read book Localized Surface Plasmon Resonance Spectroscopy of Gold and Silver Nanoparticles and Plasmon Enhanced Flurescence written by Elizabeth Anne Vokac and published by . This book was released on 2011 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis presents spectroscopic studies of metallic nanoparticle localized surface plasmons and plasmon enhanced fluorescence. We investigated the dielectric sensitivity of silver nanoprisms to an external electric field and gold nanorods to the formation of a self-assembled surface monolayer. Dark field microscopy was used to image plasmonic scattering from single nanoparticles, and a liquid crystal tunable filter was used to construct corresponding spectra. The plasmon resonances of silver nanoprisms displayed both reversible red shifts and irreversible blue shifts along with drastic intensity changes upon exposure to an applied bias. The plasmon resonances of gold nanorods showed sensitivity to the presence of alkanethiol molecules adhered to the particle surface by a moderate red shift. An increase in the effective external dielectric caused a shift toward longer wavelengths. We imaged plasmon enhanced fluorescence in order to optimize experimental parameters for a developing project that can characterize nanoparticle structure on sub-wavelength dimensions. Preliminary controls were performed to account for the effect of O2 plasma treatment, solvent and alkanethiol monolayer formation on surface plasmon resonances. We found that O2 plasma treatment for different time intervals did not result in a plasmon shift compared to untreated nanoparticles exposed to N2; however when exposed to solvent the surface plasmons of the treated particles shifted five times as far toward the red. Interestingly, the solvent effect only resulted in a plasmon shift when the particles were N2 dried after solvent incubation. Gold nanorods incubated in ethanol showed no wavelength maximum shift in pure solvent over time, but shifted moderately to the red after incubation in a solution of alkanethiol molecules. Conditions for the plasmon enhanced fluorescence study were optimized using a dye conjugate of the same alkanethiol molecule used previously by formation from solution in a monolayer on the gold nanorod surface. The appropriate synthesis for dye functionalization, molecular concentrations, solvents and optical settings were determined.

Download or read book Surface Plasmon Enhanced Fluorescence for Biological Imaging written by Shengnan Huang (Ph.D.) and published by . This book was released on 2020 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence imaging offers high spatio-temporal resolution, low radiation dosage exposure, and low cost among all the available imaging modalities, for example, magnetic resonance imaging, computerized tomography and positron emission tomography. Imaging probes of high emissivity and photostability are the key to achieving fluorescence imaging with high signal-to-background ratio (SBR). One promising approach to developing highly bright and stable imaging probes is through surface plasmon enhanced fluorescence. In the first part of the thesis, we develop a fluorescent probe with high site-specificity and emission efficiency by exploiting the targeting-specificity of M13 virus and co-assembling plasmonic nanoparticles and visible dye molecules on the viral capsid. Practical factors controlling fluorescence enhancement, such as nanoparticle size and dye-to-nanoparticle distance, are studied in this project. Lastly, the highly fluorescent probe is applied for in vitro staining of E. coli. The methodology in this work is amendable to developing a wide range of affinity-targeted fluorescent probes using biotemplates. Compared to visible and near infrared spectrum, short-wave infrared (SWIR, 900-1700 nm) spectrum promises high spatial resolution and deep tissue penetration for fluorescence imaging of biological system, owning to low tissue autofluorescence and suppressed tissue scattering at progressively longer wavelengths. In the second part of the thesis, a bright SWIR imaging probe consisting of small SWIR dyes and gold nanorods is developed for in vivo imaging. Fluorescence enhancement is optimized by tuning the dye density on the gold nanorod surface. The SWIR imaging probes are applied for in vivo imaging of ovarian cancer. The effect of targeting modality on intratumor distribution of the imaging probes is studied in two different orthotopic ovarian cancer models. Lastly, we demonstrate that the plasmon enhanced SWIR imaging probe has great potential for fluorescence imaging-guided surgery by showing its capability to detect submillimeter-sized tumors. Apart from enhancing the SWIR down-conversion emission above, surface plasmon enhanced SWIR up-conversion emission is another promising approach to achieving "autofluorescence-free" imaging with minimal tissue scattering. In the third part of the thesis, we use gold nanorods to enhance the up-conversion emission of small SWIR dyes. The mechanism of surface plasmon enhanced up-conversion emission is studied. The up-conversion fluorescence shows much higher SBR than down-conversion fluorescence in non-scatting biological solution and scatting medium. Lastly, we demonstrate in vivo imaging for the first-time using SWIR up-conversion fluorescence with exceptional image contrast.

Download or read book Biophotonics written by Jürgen Popp and published by John Wiley & Sons. This book was released on 2008-09-08 with total page 624 pages. Available in PDF, EPUB and Kindle. Book excerpt: An account of a three-year research program funded by the German government, in which physicists and physical chemists set off together with biologists and physicians to develop new techniques for medical and biological problems and ended up with sophisticated scientific solutions and innovative equipment, partly ready for the market. It not only includes a concise description of the new discoveries but also offers also an introduction to the various fields within optics.

Download or read book Advances in Medical Imaging Detection and Diagnosis written by Raj Bawa and published by CRC Press. This book was released on 2023-10-18 with total page 1426 pages. Available in PDF, EPUB and Kindle. Book excerpt: Medical care is the most critical issue of our time and will be so for the foreseeable future. In this regard, the pace and sophistication of advances in medicine in the past two decades have been truly breathtaking. This has necessitated a growing need for comprehensive reference resources that highlight current issues in specific sectors of medicine. Keeping this in mind, each volume in the Current Issues in Medicine series is a stand‐alone text that provides a broad survey of various important topics in a focused area of medicine—all accomplished in a user-friendly yet interconnected format. This volume addresses advances in medical imaging, detection, and diagnostic technologies. Technological innovations in these sectors of medicine continue to provide for safer, more accurate, and faster diagnosis for patients. This translates into superior prognosis and better patient compliance, while reducing morbidity and mortality. Hence, it is imperative that practitioners stay current with these latest advances to provide the best care for nursing and clinical practices. While recognizing how expansive and multifaceted these areas of medicine are, Advances in Medical Imaging, Detection, and Diagnosis addresses crucial recent progress, integrating the knowledge and experience of experts from academia and the clinic. The multidisciplinary approach reflected makes this volume a valuable reference resource for medical practitioners, medical students, nurses, fellows, residents, undergraduate and graduate students, educators, venture capitalists, policymakers, and biomedical researchers. A wide audience will benefit from having this volume on their bookshelf: health care systems, the pharmaceutical industry, academia, and government.

Download or read book Advanced Schemes for Surface Plasmon Resonance and Plasmon enhanced Fluorescence Biosensors written by Chun-Jen Huang and published by . This book was released on 2010 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Grating Coupled Surface Plasmon Enhanced Fluorescence Spectroscopy written by and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last three decades, sensors based on the phenomenon of surface plasmon resonance have proven particularly suitable for real time thin film characterization, gas detection, biomolecular interaction examination and to supplement electrochemical methods. Systems based on prism coupling have been combined with fluorescence detection under the name of surface plasmon fluorescence spectroscopy to increase sensitivity even further. Alternatively, metal gratings can be employed to match photons for plasmon resonance. The real time monitoring of binding reactions not yet been reported in the combination of fluorescence detection and grating coupling. Grating-based systems promise more competitive products, because of reduced operating costs, and offer benefits for device engineering. This thesis is comprised of a comprehensive study of the suitability of grating coupling for fluorescence based analyte detection. Fundamental properties of grating coupled surface plasmon fluorescence spectroscopy are described, as well as issues related to the commercial realization of the method. Several new experimental techniques are introduced and demonstrated in order to optimize performance in certain areas and improve upon capabilities in respect to prism-based systems. Holographically fabricated gratings are characterized by atomic force microscopy and optical methods, aided by simulations and profile parameters responsible for efficient coupling are analyzed. The directional emission of fluorophores immobilized on a grating surface is studied in detail, including the magnitude and geometry of the fluorescence emission pattern for different grating constants and polarizations. Additionally, the separation between the minimum of the reflected intensity and the maximum fluorescence excitation position is examined. One of the key requirements for the commercial feasibility of grating coupling is the cheap and faithful mass production of disposable samples from a given master grat.

Download or read book Handbook of Surface Plasmon Resonance written by Richard B. M. Schasfoort and published by Royal Society of Chemistry. This book was released on 2017-05-30 with total page 555 pages. Available in PDF, EPUB and Kindle. Book excerpt: Surface plasmon resonance (SPR) plays a dominant role in real-time interaction sensing of biomolecular binding events, this book provides a total system description including optics, fluidics and sensor surfaces for a wide researcher audience.

Download or read book Multidisciplinary Microfluidic and Nanofluidic Lab on a Chip written by Xiujun (James) Li and published by Newnes. This book was released on 2021-09-19 with total page 486 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multidisciplinary Microfluidic and Nanofluidic Lab-on-a-Chip: Principles and Applications provides chemists, biophysicists, engineers, life scientists, biotechnologists, and pharmaceutical scientists with the principles behind the design, manufacture, and testing of life sciences microfluidic systems. This book serves as a reference for technologies and applications in multidisciplinary areas, with an emphasis on quickly developing or new emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology. The book offers practical guidance on how to design, analyze, fabricate, and test microfluidic devices and systems for a wide variety of applications including separations, disease detection, cellular analysis, DNA analysis, proteomics, and drug delivery. Calculations, solved problems, data tables, and design rules are provided to help researchers understand microfluidic basic theory and principles and apply this knowledge to their own unique designs. Recent advances in microfluidics and microsystems for life sciences are impacting chemistry, biophysics, molecular, cell biology, and medicine for applications that include DNA analysis, drug discovery, disease research, and biofluid and environmental monitoring. Provides calculations, solved problems, data tables and design rules to help understand microfluidic basic theory and principles Gives an applied understanding of the principles behind the design, manufacture, and testing of microfluidic systems Emphasizes on quickly developing and emerging areas, including digital microfluidics, nanofluidics, papers-based microfluidics, and cell biology

Download or read book Nanoplasmonic Sensors written by Alexandre Dmitriev and published by Springer Science & Business Media. This book was released on 2012-07-31 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a compendium of the finest research in nanoplasmonic sensing done around the world in the last decade. It describes basic theoretical considerations of nanoplasmons in the dielectric environment, gives examples of the multitude of applications of nanoplasmonics in biomedical and chemical sensing, and provides an overview of future trends in optical and non-optical nanoplasmonic sensing. Specifically, readers are guided through both the fundamentals and the latest research in the two major fields nanoplasmonic sensing is applied to – bio- and chemo-sensing – then given the state-of-the-art recipes used in nanoplasmonic sensing research.

Download or read book Biomedical Photonics Handbook Second Edition written by Tuan Vo-Dinh and published by CRC Press. This book was released on 2014-07-29 with total page 894 pages. Available in PDF, EPUB and Kindle. Book excerpt: Shaped by Quantum Theory, Technology, and the Genomics Revolution The integration of photonics, electronics, biomaterials, and nanotechnology holds great promise for the future of medicine. This topic has recently experienced an explosive growth due to the noninvasive or minimally invasive nature and the cost-effectiveness of photonic modalities in medical diagnostics and therapy. The second edition of the Biomedical Photonics Handbook presents fundamental developments as well as important applications of biomedical photonics of interest to scientists, engineers, manufacturers, teachers, students, and clinical providers. The second volume, Biomedical Diagnostics, focuses on biomedical diagnostic technologies and their applications from the bench to the bedside. Represents the Collective Work of over 150 Scientists, Engineers, and Clinicians Designed to display the most recent advances in instrumentation and methods, as well as clinical applications in important areas of biomedical photonics to a broad audience, this three-volume handbook provides an inclusive forum that serves as an authoritative reference source for a broad audience involved in the research, teaching, learning, and practice of medical technologies. What’s New in This Edition: A wide variety of photonic biochemical sensing technologies have already been developed for clinical monitoring of physiological parameters, such as blood pressure, blood chemistry, pH, temperature, and the presence of pathological organisms or biochemical species of clinical importance. Advanced photonic detection technologies integrating the latest knowledge of genomics, proteomics and metabolomics allow sensing of early disease state biomarkers, thus revolutionizing the medicine of the future. Nanobiotechnology has opened new possibilities for detection of biomarkers of disease, imaging single molecules and in situ diagnostics at the single cell level. In addition to these state-of-the art advancements, the second edition contains new topics and chapters including: • Fiber Optic Probe Design • Laser and Optical Radiation Safety • Photothermal Detection • Multidimensional Fluorescence Imaging • Surface Plasmon Resonance Imaging • Molecular Contrast Optical Coherence Tomography • Multiscale Photoacoustics • Polarized Light for Medical Diagnostics • Quantitative Diffuse Reflectance Imaging • Interferometric Light Scattering • Nonlinear Interferometric Vibrational Imaging • Multimodality Theranostics Nanoplatforms • Nanoscintillator-Based Therapy • SERS Molecular Sentinel Nanoprobes • Plasmonic Coupling Interference Nanoprobes Comprised of three books: Volume I: Fundamentals, Devices, and Techniques; Volume II: Biomedical Diagnostics; and Volume III: Therapeutics and Advanced Biophotonics, this second edition contains eight sections, and provides introductory material in each chapter. It also includes an overview of the topic, an extensive collection of spectroscopic data, and lists of references for further reading.

Download or read book Handbook of Molecular Plasmonics written by Fabio Della Sala and published by CRC Press. This book was released on 2013-08-13 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt: While several reviews and books on surface nanophotonics and fluorescence spectroscopy are available, an updated focus on molecular plasmonics, including both theoretical methods and experimental aspects, is still lacking. This handbook is a comprehensive overview on the physics of the plasmon–emitter interaction, ranging from electromagnetism to quantum mechanics, from metal-enhanced fluorescence to surface-enhanced Raman scattering, from optical microscopy to synthesis of metal nanoparticles, filling the gap in the literature of this merging field. It allows experimentalists to have a solid theoretical reference at a different level of accuracy, and theoreticians to find new stimuli for novel computational methods and emerging applications.

Download or read book Surface Plasmon Fluorescence Spectroscopy and Microscopy Studies for Biomolecular Interaction Studies written by and published by . This book was released on 2005 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: ABSTARCT Biotechnology has enabled the modification of agricultural materials in a very precise way. Crops have been modified through the insertion of new traits or the inhibition of existing gene functions, named Genetically Modified Organism (GMO), and resulted in improved tolerance of herbicide and/or increased resistance against pests, viruses and fungi. Commercial cultivation of GMO started in 1996 and increased rapidly in 2003 according to a recently released report by the International Service for the Acquisition of Agri-Biotech Applications (ISAAA), depicted continuing consumer resistance in Europe and other part of the world. Upon these developments, the European Union regulations mandated labeling of GMOs containing food and as a consequence, the labeling of GMO containing product in the case of exceeding the1% threshold of alien DNA is required. The aim of the study is to be able to detect and quantify the GMO from the mixture of natural food components. The surface plasmon resonance (SPR) technique combined with fluorescence was used for this purpose. During the presented studies, two key issues are addressed and tried to solve; what is the best strategy to design and built an interfacial architecture of a probe oligonucletide layer either on a two dimensional surface or on an array platform; and what is the best detection method allowing for a sensitive monitoring of the hybridisation events. The study includes two parts: first part includes characterization of different PNAs on a 2D planar surface by defining affinity constants using the very well established optical method "Surface Plasmon Fluorescence Spectroscopy"(SPFS) and on the array platform by "Surface Plasmon Fluorescence Microscopy" (SPFM), and at the end comparison of the sensitivity of these two techniques. The second part is composed of detection of alien DNA in food components by using DNA and PNA catcher probes on the array platform in real-time by SPFM.