Download or read book Subnuclear Components written by G. D. Birnie and published by Elsevier. This book was released on 2016-01-21 with total page 349 pages. Available in PDF, EPUB and Kindle. Book excerpt: Subnuclear Components: Preparation and Fractionation focuses on the isolation of subnuclear components of eukaryotic cells. The book first discusses the isolation of nuclear envelopes from whole tissues. Topics include nuclear envelope in situ; general consideration and evaluation of isolation methods; and nuclear envelope isolation methods. The text describes the isolation of nucleoli, including the isolation of nucleoli from oocytes, nuclei, and physarum, and isolation of plant nucleoli. The book focuses on the preparation, characterization, and fractionation of chromatin. Emphasis is on the preparation of chromatin from interphase and metaphase cells; contaminants of chromatin; characteristics of isolated chromatin; and dissociation and reassociation of chromatin. The text also discusses fractionation and isolation of histones, including the preparation of individual histone fractions and isolation and purification of the five main histone fractions of calf thymus during one preparation. The book also looks at the preparation and properties of chromatin non-histone proteins, including isolation of nuclear RNA and isolation of DNA from eukaryotic cells. The selection is a good source of data for readers interested in the isolation of subnuclear constituents of eukaryotic cells.
Download or read book The Nucleus written by Ronald Hancock and published by Humana Press. This book was released on 2008-08-28 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development of the new, powerful biochemical and biophysical methodologies described here are essential in this quest. In The Nucleus, researchers from more than forty leading international laboratories describe state-of-the-art methods for isolating nuclei and their components and for studying their structure and activities, including some pathology-associated features. Volume I: Nuclei and Subnuclear Structures presents an overview of features of the intranuclear environment, followed by the most recent procedures for isolating nuclei from a wide range of cell types including muscle cells, yeast, oocytes, cells with polytene nuclei, Arabidopsis, trypanosomes, and dinoflagellates. The latest methods are described for isolating and working with nucleoli, constitutive heterochromatin, pathology-associated inclusions, and chromatin and for examining glycosylation, sumoylation, and ADP-ribosylation of nuclear proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain lists of necessary materials and reagents, readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls. The Nucleus, Volume I: Nuclei and Subnuclear Structures is an essential reference for scientists who are working on our rapidly growing understanding of the cell nucleus and its activities.
Download or read book The Nucleus written by Ronald Hancock and published by Humana Press. This book was released on 2010-12-10 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although our understanding of the structure and activities of the cell nucleus and of the nanomachines which it contains is increasing rapidly, much remains to be learned. The application and continuing development of the new, powerful biochemical and biophysical methodologies described here are essential in this quest. In The Nucleus, researchers from more than forty leading international laboratories describe state-of-the-art methods for isolating nuclei and their components and for studying their structure and activities, including some pathology-associated features. Volume I: Nuclei and Subnuclear Structures presents an overview of features of the intranuclear environment, followed by the most recent procedures for isolating nuclei from a wide range of cell types including muscle cells, yeast, oocytes, cells with polytene nuclei, Arabidopsis, trypanosomes, and dinoflagellates. The latest methods are described for isolating and working with nucleoli, constitutive heterochromatin, pathology-associated inclusions, and chromatin and for examining glycosylation, sumoylation, and ADP-ribosylation of nuclear proteins. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain lists of necessary materials and reagents, readily reproducible protocols, and tips for troubleshooting and avoiding known pitfalls. The Nucleus, Volume I: Nuclei and Subnuclear Structures is an essential reference for scientists who are working on our rapidly growing understanding of the cell nucleus and its activities.
Download or read book The Nucleus written by Ronald Hancock and published by Humana Press. This book was released on 2014-10-14 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents detailed, recently-developed protocols ranging from isolation of nuclei to purification of chromatin regions containing single genes, with a particular focus on some less well-explored aspects of the nucleus. The methods described include new strategies for isolation of nuclei, for purification of cell type-specific nuclei from a mixture, and for rapid isolation and fractionation of nucleoli. For gene delivery into and expression in nuclei, a novel gentle approach using gold nanowires is presented. As the concentration and localization of water and ions are crucial for macromolecular interactions in the nucleus, a new approach to measure these parameters by correlative optical and cryo-electron microscopy is described. The Nucleus, Second Edition presents methods and software for high-throughput quantitative analysis of 3D fluorescence microscopy images, for quantification of the formation of amyloid fibrils in the nucleus, and for quantitative analysis of chromosome territory localization. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, The Nucleus, Second Edition seeks to serve both professionals and novices with its well-honed methods for the study of the nucleus.
Download or read book Current Catalog written by National Library of Medicine (U.S.) and published by . This book was released on 1979 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: First multi-year cumulation covers six years: 1965-70.
Download or read book Nuclear Structures written by A. J. MacGillivray and published by Elsevier. This book was released on 2013-10-22 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nuclear Structures: Isolation and Characterization focuses on techniques for the isolation of molecular and macromolecular components of cell nuclei. The book first discusses nuclear matrices, including isolation of nuclear matrices and notes on the method. The text examines nucleoids. Nucleoid preparation and morphology, characterization of nucleoids, experimental uses of nucleoids, and criticism of nucleoid preparation are underscored. The book also discusses nuclear envelopes, polynucleosomes and monomer nucleosomes, and metaphase chromosomes. The text also examines DNA tight-binding proteins, including the definition and preparation of DNA tight-binding proteins; immunological characterization of tight-binding proteins; and DNA binding characteristics. The book also examines nucleoli and preribosomal ribonucleoprotein particles. Methods for the isolation of nucleoli; assessment of nucleolar preparations; and isolation of preribosomal ribonucleoprotein particles are discussed. The text also covers ribonucleoprotein particles containing heterogeneous nuclear RNA and nuclear RNP particles containing small RNAs. The selection is a good reference for readers wanting to study the isolation of molecular and macromolecular components of cell nuclei.
Download or read book Subcellular Fractionation written by John M. Graham and published by Oxford University Press. This book was released on 1997 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the fast changing world of cell biology it is often required that particular membranes or subcellular organisms be isolated from the structure for investigative purposes. This book shows how to do this in a practical, hands-on way.
Download or read book Cellular Mechanotransduction written by Mohammad R. K. Mofrad and published by Cambridge University Press. This book was released on 2014-07-31 with total page 668 pages. Available in PDF, EPUB and Kindle. Book excerpt: 'Mechanotransduction' is the term for the ability, first described by 19th-century anatomist Julius Wolff, of living tissues to sense mechanical stress and respond by tissue remodeling. More recently, the scope of mechanotransduction has been expanded to include the sensation of stress, its translation into a biochemical signal, and the sequence of biological responses it produces. This book looks at mechanotransduction in a more restricted sense, focusing on the process of stress sensing and transducing a mechanical force into a cascade of biochemical signals. This stress has become increasingly recognized as one of the primary and essential factors controlling biological functions, ultimately affecting the function of the cells, tissues, and organs. A primary goal of this broad book is also to help define the new field of mechanomics, which attempts to describe the complete mechanical state of a biological system.
Download or read book Statistical Models for Nucleon Structure Function written by Carlos Alberto Mirez Tarrillo and published by Cambridge Scholars Publishing. This book was released on 2024-01-22 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: The structure function describes how energy/momentum is distributed among the quarks/gluon inside a nucleon. This is estimated from the deep inelastic scattering of electrons or muon with the nucleon, or even with nucleon-nucleon scattering. This book reviews several statistic/thermodynamic models for both polarized and unpolarized structure function, with additional applications, such as the study of the EMC effect. It will appeal to both researchers and students of hadronic and nuclear/particle physics.
Download or read book Fundamental Nuclear Energy Research written by U.S. Atomic Energy Commission. Division of Plans and Reports and published by . This book was released on 1968 with total page 396 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book TID written by and published by . This book was released on 1966 with total page 540 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book The Electromagnetic Fields of Consciousness written by James F Pagel and published by Elsevier. This book was released on 2024-06-01 with total page 318 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Electrical Fields of Consciousness: Brain Waves and Dreaming examines neuro-electrophysiology from its origins in the electrical fields of primitive organisms to its roles in the electrophysiologically defined states of human consciousness. This volume presents and cogently organizes the wide spectrum of information available as to how and why bioelectrical fields are used to define and function in supporting both life and consciousness. Integrating electrophysiology in a comprehensible manner into neuroanatomical, electromagnetic, and global platform theories of neuroconsciousness, this book brings together the wide spectrum of available data-based evidence regarding the physiology and functions of bioelectric fields and their relationship to consciousness. Chapters examine the origin and potential function of bioelectrical fields and EEG associations with conscious states, as well as the relationship between electrical fields and consciousness. Incorporating the basic science, clinical correlates, and functional potential of CNS bioelectrical fields, this book is a must-read for all working in the field of CNS electrophysiology or neuroconsciousness. Addresses our current understandings of the origin and potential functions of bioelectricity Integrates electrophysiology into current theories of neuroconsciousness, presenting within an historic research and clinical context Explores the origin, nature, bioenergetics, and potential genetic effects of synchronous physiologic electrical fields and their use as markers for life and death Addresses the definition and assessment of consciousness in human and non-human systems and reviews electrophysiological markers of wake and sleep consciousness states Discusses electrophysiologic drug and disease correlates as well as modern forms of electrical therapy
Download or read book The Lattice Boltzmann Equation written by Sauro Succi and published by Oxford University Press. This book was released on 2018 with total page 789 pages. Available in PDF, EPUB and Kindle. Book excerpt: An introductory textbook to Lattice Boltzmann methods in computational fluid dynamics, aimed at a broad audience of scientists working with flowing matter. LB has known a burgeoning growth of applications, especially in connection with the simulation of complex flows, and also on the methodological side.
Download or read book Methods in Cell Biology written by and published by Academic Press. This book was released on 1970-04-30 with total page 561 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Cell Biology
Download or read book Chromatin and Chromosomal Protein Research II written by and published by Academic Press. This book was released on 1978-07-26 with total page 455 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromatin and Chromosomal Protein Research II
Download or read book Metallic Glass Based Nanocomposites written by Sumit Sharma and published by CRC Press. This book was released on 2019-09-23 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt: Metallic Glass-Based Nanocomposites: Molecular Dynamics Study of Properties provides readers with an overview of the most commonly used tools for MD simulation of metallic glass composites and provides all the basic steps necessary for simulating any material on Materials Studio. After reading this book, readers will be able to model their own problems on this tool for predicting the properties of metallic glass composites. This book provides an introduction to metallic glasses with definitions and classifications, provides detailed explanations of various types of composites, reinforcements and matrices, and explores the basic mechanisms of reinforcement-MG interaction during mechanical loading. It explains various models for calculating the thermal conductivity of metallic glass composites and provides examples of molecular dynamics simulations. Aimed at students and researchers, this book caters to the needs of those working in the field of molecular dynamics (MD) simulation of metallic glass composites.
Download or read book Plant Biochemistry written by James Bonner and published by Elsevier. This book was released on 2013-10-22 with total page 1073 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Biochemistry focuses on the biological processes involved in plants, particularly noting metabolism, electron transport, biogenesis, and germination. The manuscript first offers information on the substructures and subfunctions of plant cell, including cell and subcell, enzymes, ribosomes, nucleus, cellular membranes, mitochondria and electron transport, chloroplast, and the substructure and function of the cell wall. The text then elaborates on basic metabolism. Enzymology, the path of carbon in respiratory metabolism, mono- and oligosaccharides, starch, insulin, and other reserve polysaccharides, and the biogenesis of the cell wall are discussed. The publication explains plant metabolism and control. Discussions focus on plant acids, alkaloid biogenesis, coumarins, phenylpropanes, and lignin, ethylene and polyacetylenes, steroids, and seed development and germination. The book is a valuable source of information for students or professional workers in the plant sciences.
