Download or read book Studies on the Function of the PRD 1 Gene in Neurospora Crassa written by Di Wu and published by . This book was released on 2018 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Neurospora crassa, conidiation is controlled by the circadian clock which consists of more than one oscillator. The frequency (frq) gene is a key component in the FRQ oscillator. The prd-1 mutant was found to lengthen the period of the circadian rhythm and disrupts the FRQ-less rhythm, but how this mutant affects the oscillators was unclear. This thesis aims to study the function of the prd-1 gene. Building on previous research, knocking out prd-1 is lethal; strains expressing PRD-1-FLAG were constructed; prd-1 expression showed non- circadian rhythmicity; co-IP and MS analysis discovered ribosomal proteins bind to PRD-1; the prd-1 mutant responds to amino acids differently in growth; and the deletion of the C-terminus of prd-1 was found to be lethal. The results of these various experiments outlined that the prd-1 gene may affect the circadian rhythm via nutrient sensing pathways especially the TOR pathway.

Download or read book Characterization of PRD 1 Mutation in Neurospora Crassa written by Ghazaleh Firoozi and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Transformation of Neurospora Crassa with the Trp 1 Gene written by Soo Young Kim and published by . This book was released on 1987 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic Studies of Resistance to Chemical Agents in Neurospora Crassa written by Claude Emory Terry and published by . This book was released on 1962 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Studies on the Cellulase Genes of Neurospora Crassa written by Fawzi Taleb and published by . This book was released on 1993 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book The Role of the Cross Pathway Control cpc 2 Gene in the Filamentous Fungus Neurospora Crassa written by Amruta Vikas Garud and published by . This book was released on 2013 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt: Previous work demonstrated that CPC-2 plays an important role during general amino acid control in N. crassa, along with having a role in overall growth and female fertility. My research investigated a possible role for cpc-2 in the G protein signaling pathway, and also investigated genetic epistasis between cpc-2, gnb-1 and the G[Alpha] genes in N. crassa .

Download or read book Fine Structure in the Histidine 1 Gene of Neurospora Crassa written by Adrienne Patricia Jessop and published by . This book was released on 1964 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Expression of Genes in Neurospora Crassa Outside of the Quinic Acid Gene Cluster During Quinic Acid Metabolism written by John William Savopoulos and published by . This book was released on 2018 with total page 170 pages. Available in PDF, EPUB and Kindle. Book excerpt: The quinic acid (qa) gene cluster in Neurospora crassa (N. crassa) consists of two regulatory genes (qa-1F and qa-1S) as well as five structural genes (qa-2, qa-3, qa-4, qa-y, and qa-x) that are transcribed in the presence of quinic acid with suppressed levels of glucose (Greever R.F., et al. 1988). The corresponding activation and repression of this cluster enables the survival of the organism in less than hospitable environments. A research study hypothesized that some 50 genes were under the control of the qa-1F gene and another qa transcription factor. Furthermore, it was shown at the proteomic level in the work of Kayla Brown, Katie Allen, and Dana Tirabassi that glycogen phosphorylase (NCU07027), peptidyl-prolyl cis-trans isomerase (NCU04140), and NCU08332 Hex-1: Woronin Body Coding Protein all had heightened expression at the proteomic level in the presence of quinic acid. Therefore, the scope of this work aimed to analyze these three genes to determine their levels of transcription in the presence of quinic acid (utilizing qRT-PCR), and to determine if they are related to the quinic acid gene cluster. The results indicate that heightened glycogen phosphorylase expression seen in prior works is not related to quinic acid metabolism and is not a result of starvation. However, it is predicted that the cell may be holding on to this protein for other purposes not related to the scope of this work. Peptidyl prolyl cis-trans isomerase appeared to not be related to the quinic acid gene cluster, but rather showed heightened expression as a result of starvation. Last, the NCU08332 Hex-1 Woronin body major protein gene appeared to have a direct relation to quinic acid metabolism and the quinic acid gene cluster. Furthermore, bioinformatics analyses presented also supported this assumptions.

Download or read book Differential Gene Expression in Neurospora Crassa Cell Types written by and published by . This book was released on 1980 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The significant results obtained during 1979 to 1980 of the current research program are as follows: (1) the differential rRNA gene amplification in germinated conidia of N.crassa was confirmed. N.crassa rDNAs showed differences in degrees of homology with isolated DNAs from other Neurospora species which could be due to heterogeneity in internal spacers. Studies with N.crassa rDNA clones were initiated to study their heterogeneities. The organization of the Institutional Biohazard Committee (IBC) for Recombinant DNA research was completed and necessary certifications for the laboratory and the workers were obtained in accordance with the P2EK1 containment regulation of N.I.H. Known 17S and 26S N.crassa rDNA probes are being used to detect differences, if any, in restriction cleavage sites in rDNAs of different cell types and developmental mutants of N.crassa. DNAs from these N.crassa cells are restricted with EcoR1 and Hind III and cleaved fragments separated by gel electrophoresis are transferred into nitrocellulose papers. Experiments are underway now to see if there are any changes in cleavage sites by annealing with 32P or 3H-17S or 26S rDNA probes followed by autoradiography.

Download or read book Molecular Dissection of a Genome Defense Pathway in Neurospora Crassa written by Erin C. Boone and published by . This book was released on 2015 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: Meiotic silencing by unpaired DNA (MSUD) is an RNA interference (RNAi) pathway in Neurospora crassa that detects genes without a homologous partner and silences them for the duration of sexual development. There are currently nine characterized MSUD genes, seven of which produce proteins that localize in a ring-like pattern around the nucleus. The perinuclear region is known to be a hub for RNAi-related proteins in mouse, fly, and worm germ cells (known as chromatoid body, nuage, and Pgranules, respectively). In this study, we have further elucidated the function of known MSUD proteins, identified novel proteins that are required for MSUD, and demonstrated the conservation of RNAi-related processes at the nuclear periphery. We began by showing SAD-2 is crucial for the localization of other MSUD proteins in the perinuclear region. Previous data have revealed that SAD-2 is required to shuttle SAD-1 (RNA-directed RNA polymerase) to its proper location. In our experiments, a SAD-2-null background causes SAD-3 (helicase), DCL-1 (Dicer), QIP (exonuclease), and SMS-2 (Argonaute) to also lose their affinity for the nuclear periphery, while in the absence of SAD-1 or SAD-3, SAD-2 still localizes normally. These data suggest that SAD-2 works as a scaffold protein and that proper function of MSUD, like other germline RNAi-like systems, is reliant on the presence of silencing proteins in the perinuclear region. An MSUD suppression assay identified two novel MSUD proteins, SAD-Y and SAD-B'. Even though SAD-Y and its homologs contain a conserved putative RNA-binding motif, they have yet to be assigned to a biochemical pathway. Our work here has linked a putative function (i.e., silencing) to SAD-Y-like proteins. SAD-Y has been shown to interact with other MSUD factors in both the nucleus and at the nuclear periphery. SAD-B'’s homolog has been found in the nuage, an epicenter for RNA-binding proteins involved in post-transcriptional regulation for Drosophila germline cells. Transcript analysis showed that sad-b' is mainly expressed in the sexual cycle. SAD-B' interacts with core MSUD proteins and has an especially intimate association with SMS- 2, which requires it for localization. Furthermore, bimolecular fluorescence complementation (BiFC) revealed that SAD-B' interacts with a Golgi retrograde transport protein and an autophagy marker protein, suggesting the importance of the endomembrane system in this RNAi process.

Download or read book Functional Aspects of the Pyrimidine 3 Gene of Neurospora Crassa written by D. J. Rigby and published by . This book was released on 1982 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Changes in Gene Expression of Neurospora Crassa in Response to Quinic Acid written by Kayla A. Brown and published by . This book was released on 2016 with total page 82 pages. Available in PDF, EPUB and Kindle. Book excerpt: As a filamentous fungus, Neurospora crassa serves as an ideal model for eukaryotic organisms. Like many fungi, Neurospora is able to utilize many different carbon sources for energy. This however, requires the presence of genes that code for a variety of metabolic pathways that are not always needed. An example of such a group of genes would be the genes involved in utilizing quinic acid. When Neurospora grows in the presence of a less preferred carbon source, such as quinic acid, gene expression of the quinic acid (qa) gene cluster is up-regulated. This allows the organism to metabolize quinic acid and survive in the less favorable conditions. In contrast, when in the presence of a preferred carbon source, such as dextrose or sucrose, the qa genes are repressed. This study examines how changing the carbon source effects gene expression in wild-type N.crassa. N.crassa was first grown in presence of either quinic acid or sucrose and harvested for tissue. Then, protein was extracted from this tissue and analyzed by 1- Dimensional Gel Electrophoresis (1-DGE). Differences in protein expression was compared using the Quantity One® 1-D analysis software. Proteins unique to growth on quinic acid were identified after being submitted for mass spectrometry. Finally, gene transcription was quantitated in order to determine which genes coded for proteins expressed only in the presence of quinic acid by reverse transcriptase polymerase chain reaction (RT-PCR) and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR).

Download or read book Characterization of the Neurospora Crassa Cell Wall and Glycosylphosphatidylinositol GPI anchor Biosynthetic Pathways written by and published by . This book was released on 2006 with total page 165 pages. Available in PDF, EPUB and Kindle. Book excerpt: The cell wall plays a vital role in the physiology of the filamentous fungus Neurospora crassa, but its synthesis, structure, and remodeling dynamics have not been well defined. To this end, N. crassa mutants affected in two biosynthetic pathways involved in cell wall formation, protein mannosylation and glycosylphosphatidylinositol (GPI)-anchor addition, have been isolated and characterized. These pathways are important for the production of cell wall glycoproteins, which are involved in the synthesis, assembly, organization, and remodeling of the cell wall. The mutants exhibit reduced rates of growth, altered hyphal and gross colony morphologies, and pronounced cell wall defects. In addition, these cell wall mutants are unable to produce many of the characteristic cell types formed as part of the normal N. crassa life cycle. In chapter 2, a novel genetic mapping and PCR-based sequencing assay was used to the clone the mnt-1 gene, which encodes an alpha-1,2-mannosyltransferase. The mnt-1 gene was shown to be required for the synthesis of the galactomannan component present on glycoproteins in the cell wall. Mnt-1 mutants have altered cell wall carbohydrate composition and are unable to repress the onset of the asexual developmental program. The work in chapter 3 details the identification of the gpip-1, gpip-2, gpip-3, and gpit-1 genes, which are involved in the addition of the GPI-anchor to select cell wall glycoproteins. The gpip-1, gpip-2, and gpip-3 genes encode phosphoethanolamine transferases that function in the addition of phosphoethanolamine groups to the GPI-anchor during anchor biogenesis. The gpit-1 gene encodes a component of the GPI transamidase complex involved in the transfer of the completed anchor structure to the target protein. The GPI-anchor mutants experience a significant degree of cell lysis, have altered cell wall carbohydrate and protein compositions, and are deficient in the production of a number of putative GPI-anchored cell wall proteins. The characterization of the protein component in the mutant and wild-type cell walls allowed for the identification of several GPI-anchored and non-anchored cell wall proteins. Overall, the results of this doctoral work demonstrate that the addition of galactomannan to cell wall proteins is an important element of cell wall biogenesis. The work also highlights the significance of the GPI-anchor in directing GPI-anchored proteins to the cell wall and the importance of those proteins for cell wall biosynthesis and remodeling. The addition of galactomannans and GPI-anchors are required for the normal morphology and development of N. crassa.

Download or read book Characterization of Ccg 1 a Clock controlled Gene of Neurospora Crassa written by Kristin Marguerite Lindgren and published by . This book was released on 1994 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Genetic and Biochemical Studies of Heterokaryons of Neurospora Crassa written by J. E. Ogdem and published by . This book was released on 1982 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Identification of a Genetically Unstable Strain of Neurospora Crassa as a Mutant at the Phen 1 Locus written by Krishna Kumar Jha and published by . This book was released on 1964 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: An auxotrophic strain of Neurospora crassa ( UA119), isolated after nitrous acid treatment of conidia, was earlier reported to be mutant at the tryp -3 locus. Analysis of the crosses between UA119 and fluffy show the location of the "tryptophan" mutation on the linkage group I, in the vicinity of sex . The allelic nature of the mutation in UA119 with two previously described phen -1 mutants is confirmed by the low frequency of prototrophs in the progeny of crosses with the latter and by the nutritional characteristics of UA119 (any one of the aromatic amino acids or leucine or ethyl acetoacetate is required for growth by phen -1 mutants). The viability of ascospores from "inter-allelic" crosses is low (15-20%) and ascospore isolates with modified nutritional characteristics such as inability to grow on phenylalanine-supplemented medium or a specific requirement for leucine, are frequent. Genetic instability and leakiness are characteristics exhibited by all the three phen -1 mutants but the frequency of phenotypic reversions in UA119 is very high (approx. 10 prototrophs/10^ viable conidia; colonies larger than 2 mm. on sorbose-agar medium after 3 days at 30 C. counted). The viability of conidia of the phen -1 mutants is reduced to 20% or less by L-phenylalanine, L-leucine or ethyl acetoacetate in sorbose-agar medium, whereas on L-tryptophan- or L-tyrosine-supplemented medium the viability ranges from 40 to 60 percent. The reasons for the apparent scarcity of phen -1 mutants are discussed and it is suggested that ethyl acetoacetate is the best selective compound for the isolation of phen -1 mutants. Various hypotheses with respect to the nature and the primary role of the mutant phen -1 gene are considered and it is suggested that the activity of a transaminase involved in the metabolism of phenylalanine, tyrosine and leucine might be altered as a result of mutation at the phen-1 locus.

Download or read book Brenner s Encyclopedia of Genetics written by Stanley Maloy and published by Academic Press. This book was released on 2013-03-03 with total page 4360 pages. Available in PDF, EPUB and Kindle. Book excerpt: The explosion of the field of genetics over the last decade, with the new technologies that have stimulated research, suggests that a new sort of reference work is needed to keep pace with such a fast-moving and interdisciplinary field. Brenner's Encyclopedia of Genetics, Second Edition, Seven Volume Set, builds on the foundation of the first edition by addressing many of the key subfields of genetics that were just in their infancy when the first edition was published. The currency and accessibility of this foundational content will be unrivalled, making this work useful for scientists and non-scientists alike. Featuring relatively short entries on genetics topics written by experts in that topic, Brenner's Encyclopedia of Genetics, Second Edition, Seven Volume Set provides an effective way to quickly learn about any aspect of genetics, from Abortive Transduction to Zygotes. Adding to its utility, the work provides short entries that briefly define key terms, and a guide to additional reading and relevant websites for further study. Many of the entries include figures to explain difficult concepts. Key terms in related areas such as biochemistry, cell, and molecular biology are also included, and there are entries that describe historical figures in genetics, providing insights into their careers and discoveries. This 7-volume set represents a 25% expansion from the first edition, with over 1600 articles encompassing this burgeoning field Thoroughly up-to-date, with many new topics and subfields covered that were in their infancy or not inexistence at the time of the first edition. Timely coverage of emergent areas such as epigenetics, personalized genomic medicine, pharmacogenetics, and genetic enhancement technologies Interdisciplinary and global in its outlook, as befits the field of genetics Brief articles, written by experts in the field, which not only discuss, define, and explain key elements of the field, but also provide definition of key terms, suggestions for further reading, and biographical sketches of the key people in the history of genetics