Download or read book Studies of Exon Scrambling and Mutually Exclusive Alternative Splicing written by Rong Kong and published by . This book was released on 2005 with total page 74 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goals of this thesis work were to study two special alternative splicing events: exon scrambling at the RNA splicing level and mutually exclusive alternative splicing (MEAS) by computational and experimental methods. Chapter 1 presents work on the study of exon scrambling, in which exons are spliced at canonical splice sites but joined together in an order different from that predicated by the genomic sequence. The public expressed sequence tag (EST) database was searched for transcripts containing scrambled exons. Stringent criteria were used to exclude genome annotation or assembly artifacts. This search identified 172 human ESTs representing 90 exon scrambling events, which derive from 85 different human genes. In several cases, the scrambled transcripts were validated using an RT-PCR-sequencing protocol, confirming the reproducibility of these unusual events. Exon scrambling of transcripts from the GLI3 gene, which encodes a transcription factor involved in hedgehog signaling, was also conserved in mouse. Specific gene features, including the presence of long flanking introns were found to be associated with exon scrambling. Chapter 2 deals with mutually exclusive alternative splicing (MEAS), in which only one of a set of two or more exons in a gene is included in the final transcript. A database with 101 human genes and 25 mouse genes containing mutually exclusive exons (MXE) has been established with GENOA annotation software. Specific sequence features were analyzed. A genome-wide search for a special "tandem MEAS" events was undertaken and 10 such human genes were identified. A fluorescence reporting system was built to study intronic cis-elements regulating MEAS.

Download or read book Cancer Research An Interdisciplinary Approach written by Nima Rezaei and published by Springer Nature. This book was released on 2023-07-03 with total page 614 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cancer is a major public health concern and one of the leading causes of death. There is no simple solution for this complex disease, therefore interdisciplinary approaches might help to find solutions to many unanswered questions and challenges about cancer. The rapid flow of interdisciplinary research in cancer during recent years has increased our understanding of the nature of cancers. Such interdisciplinary approaches could be helpful for both the diagnosis and the development of more effective therapeutic strategies. The “Interdisciplinary Cancer Research” series publishes comprehensive volumes on different cancers and presents the most updated and peer-reviewed articles on human cancers. Over the past decade, increased cancer research has greatly improved our understanding of the nature of cancerous cells which has led to the development of more effective therapeutic strategies to treat cancers. This interdisciplinary series is of special value to researchers and practitioners working on cell biology, immunology, hematology, biochemistry, genetics, oncology and related fields. This is the main concept of Cancer Immunology Project (CIP), which is a part of Universal Scientific Education and Research Network (USERN).

Download or read book Bioinformatics in the Era of Post Genomics and Big Data written by Ibrokhim Y. Abdurakhmonov and published by BoD – Books on Demand. This book was released on 2018-06-20 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioinformatics has evolved significantly in the era of post genomics and big data. Huge advancements were made toward storing, handling, mining, comparing, extracting, clustering and analysis as well as visualization of big macromolecular data using novel computational approaches, machine and deep learning methods, and web-based server tools. There are extensively ongoing world-wide efforts to build the resources for regional hosting, organized and structured access and improving the pre-existing bioinformatics tools to efficiently and meaningfully analyze day-to-day increasing big data. This book intends to provide the reader with updates and progress on genomic data analysis, data modeling and network-based system tools.

Download or read book Human Cell Transformation written by Johng S. Rhim and published by Springer Nature. This book was released on 2019-10-01 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book, part contributed volume, part proceedings, discusses state-of-the-art advances on human cell transformation in cell models for the study of cancer and aging. Several of the chapters are from the Human Cell Transformation: Advances in Cell Models for the Study of Cancer and Aging conference that was held in June 2018 at McGill University. The authors represent international expertise on a wide variety of topics ranging from different types of cancer (prostate, bone, breast, etc.) to tumor microenvironment, tumor progression, homogeneity, and possible therapies and treatments.

Download or read book Cancer Research written by and published by . This book was released on 2007-06 with total page 996 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Microarray Bioinformatics written by Dov Stekel and published by Cambridge University Press. This book was released on 2003-09-08 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a comprehensive guide to all of the mathematics, statistics and computing you will need to successfully operate DNA microarray experiments. It is written for researchers, clinicians, laboratory heads and managers, from both biology and bioinformatics backgrounds, who work with, or who intend to work with microarrays. The book covers all aspects of microarray bioinformatics, giving you the tools to design arrays and experiments, to analyze your data, and to share your results with your organisation or with the international community. There are chapters covering sequence databases, oligonucleotide design, experimental design, image processing, normalisation, identifying differentially expressed genes, clustering, classification and data standards. The book is based on the highly successful Microarray Bioinformatics course at Oxford University, and therefore is ideally suited for teaching the subject at postgraduate or professional level.

Download or read book Cumulated Index Medicus written by and published by . This book was released on 1991 with total page 1488 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Regulation of Alternative Splicing written by Philippe Jeanteur and published by Springer Science & Business Media. This book was released on 2002-10-21 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: The discovery in 1977 that genes are split into exons and introns has done away with the one gene - one protein dogma. Indeed, the removal of introns from the primary RNA transcript is not necessarily straightforward since there may be optional pathways leading to different messenger RNAs and consequently to different proteins. Examples of such an alternative splicing mechanism cover all fields of biology. Moreover, there are plenty of occurrences where deviant splicing can have pathological effects. Despite the high number of specific cases of alternative splicing, it was not until recently that the generality and extent of this phenomenon was fully appreciated. A superficial reading of the preliminary sequence of the human genome published in 2001 led to the surprising, and even deceiving to many scientists, low number of genes (around 32,000) which contrasted with the much higher figure around 150,000 which was previously envisioned. Attempts to make a global assessment of the use of alternative splicing are recent and rely essentially on the comparison of genomic mRNA and EST sequences as reviewed by Thanaraj and Stamm in the first chapter of this volume. Most recent estimates suggest that 40-60% of human genes might be alternatively spliced, as opposed to about 22% for C. elegans.

Download or read book Biological Agricultural Index written by and published by . This book was released on 1997 with total page 2984 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Download or read book Fuzziness written by Monika Fuxreiter and published by Springer Science & Business Media. This book was released on 2012-03-07 with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: Detailed characterization of fuzzy interactions will be of central importance for understanding the diverse biological functions of intrinsically disordered proteins in complex eukaryotic signaling networks. In this volume, Peter Tompa and Monika Fuxreiter have assembled a series of papers that address the issue of fuzziness in molecular interactions. These papers provide a broad overview of the phenomenon of fuzziness and provide compelling examples of the central role played by fuzzy interactions in regulation of cellular signaling processes and in viral infectivity. These contributions summarize the current state of knowledge in this new field and will undoubtedly stimulate future research that will further advance our understanding of fuzziness and its role in biomolecular interactions.

Download or read book The Genetic Gods written by John C. Avise and published by Harvard University Press. This book was released on 2009-06-30 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: They mastermind our lives, shaping our features, our health, and our behavior, even in the sacrosanct realms of love and sex, religion, aging, and death. Yet we are the ones who house, perpetuate, and give the promise of immortality to these biological agents, our genetic gods. The link between genes and gods is hardly arbitrary, as the distinguished evolutionary geneticist John Avise reveals in this compelling book. In clear, straightforward terms, Avise reviews recent discoveries in molecular biology, evolutionary genetics, and human genetic engineering, and discusses the relevance of these findings to issues of ultimate concern traditionally reserved for mythology, theology, and religious faith. The book explains how the genetic gods figure in our development--not just our metabolism and physiology, but even our emotional disposition, personality, ethical leanings, and, indeed, religiosity. Yet genes are physical rather than metaphysical entities. Having arisen via an amoral evolutionary process--natural selection--genes have no consciousness, no sentient code of conduct, no reflective concern about the consequences of their actions. It is Avise's contention that current genetic knowledge can inform our attempts to answer typically religious questions--about origins, fate, and meaning. The Genetic Gods challenges us to make the necessary connection between what we know, what we believe, and what we embody. Table of Contents: Preface Prologue 1. The Doctrines of Biological Science 2. Geneses 3. Genetic Maladies 4. Genetic Beneficence 5. Strategies of the Genes 6. Genetic Sovereignty 7. New Lords of Our Genes? 8. Meaning Epilogue Notes Glossary Index Reviews of this book: Our genes, [Avise] says, are responsible not only for how we got here and exist day to day, but also for the core of our being--our personalities and morals. It is our genetic make-up that allows for and formulates our religious belief systems, he argues. Avise does not eschew spirituality but seeks a more informed, less confrontational approach between science and the pulpit. --Science News Reviews of this book: For the general scientific reader, the book is an excellent distillation of a broad and increasingly important field, a course of causation that cannot be ignored. From advising expectant parents to getting innocent people off death row, genetics increasingly dominates our lives. The sections on genetics are expertly written, particularly for those readers without in-depth knowledge. The author explains slowly and carefully just how genetics operates, using multiple metaphors. His genetic discourse proceeds in a neighborly fashion, as one might tell stories while sitting in a rocking chair at a country store. He seems to be invigorated by genes and just can't wait to tell about them. --David W. Hodo, Journal of the American Medical Association Reviews of this book: As a whole, this book is quite informative and stimulating, and sections of it are beautifully written. Indeed, Professor Avise has a real gift for prose and scientific expositions, and I would suspect that he must be a formidable lecturer...At its core, [The Genetic Gods] is a survey, and a very nice one at that, of evolutionary genetics, the field of the author's major research interests. There is a strong sociobiological cast to the arguments, and the work and ideas of E. O. Wilson figure prominently. The presentation of evolutionary genetics is imbedded in a more general discussion of modern human and molecular genetics...However, this book is, most of all, a philosophical treatise that attempts, admittedly with the bias of a biologist, to examine the intersection of the fundamental premises of evolution and religion. Professor Avise has given us plenty to think about in this book [and]...it was a real pleasure to wrestle with the ideas he was presenting. I would suggest that other readers give it a try. --Charles J. Epstein, Trends in Genetics Reviews of this book: [Avise's] account of the role genes play in shaping the human condition is wholly involving, paying particular attention to issues of reproduction, aging and death. In addition to presenting ample biological information in a form accessible to the nonspecialist, Avise does a superb job of discussing many of the ethical implications that have arisen from our growing knowledge of human genetics. Just a few of the topics covered are genetic engineering, the patenting of life, genetic screening, abortion, human cloning, gene therapy and insurance-related controversies. --Publishers Weekly Reviews of this book: Avise explains thoroughly how evolution operates on a genetic level. His goal is to show that humans can look to this information as a way to answer fundamental questions of life instead of looking to traditional religious beliefs...Avise includes some very interesting discussions of ethical concerns related to genetic issues. --Eric D. Albright, Library Journal This is a splendid account of a subject that affects us all: the breathtaking increase in understanding of human genetics and the insight it provides into human evolution. John Avise speaks with authority of molecular evolutionary genetics and with affecting compassion of what it might mean. --Douglas J. Futuyma, State University of New York at Stony Brook The Genetic Gods is many things. It is a wonderful introduction to modern molecular biology, by a man who knows his subject backwards. It is a stimulating account of the ways in which genetics impinges on human nature--our thinking and our behavior. It is a remarkably level-headed and sympathetic account of the implications of our new findings for traditional and not-so-traditional issues in philosophy and religion. In an age of genetic counseling, cloning, construction of new life forms, the book is worth its weight in gold for this alone. But most of all, it is a huge amount of fun to read--you want to applaud or argue with the author on nigh every page. Highly recommended! --Michael Ruse, University of Guelph The Genetic Gods makes a valuable contribution to the on-going task of sorting out the implications of evolutionary biology and genetics for human self-understanding. Avise addresses, with authority and grace, the most consequential intellectual issues of our time. A challenging and insightful book. --Loyal Rue, Harvard University A wonderfully informative and engaging book. Avise offers a lucid, accessible primer on our genes, angelic and demonic, and examines religious and ethical issues, all too human, now confronted by genetic science. He makes a compelling case that anyone seeking to 'Know Thyself' should study the DNA molecular scriptures, our most ancient and universal legacy. --Dudley Herschbach, Harvard University, Nobel Laureate in Chemistry

Download or read book Guide to Research Techniques in Neuroscience written by Matt Carter and published by Academic Press. This book was released on 2022-03-26 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern neuroscience research is inherently multidisciplinary, with a wide variety of cutting edge new techniques to explore multiple levels of investigation. This Third Edition of Guide to Research Techniques in Neuroscience provides a comprehensive overview of classical and cutting edge methods including their utility, limitations, and how data are presented in the literature. This book can be used as an introduction to neuroscience techniques for anyone new to the field or as a reference for any neuroscientist while reading papers or attending talks. Nearly 200 updated full-color illustrations to clearly convey the theory and practice of neuroscience methods Expands on techniques from previous editions and covers many new techniques including in vivo calcium imaging, fiber photometry, RNA-Seq, brain spheroids, CRISPR-Cas9 genome editing, and more Clear, straightforward explanations of each technique for anyone new to the field A broad scope of methods, from noninvasive brain imaging in human subjects, to electrophysiology in animal models, to recombinant DNA technology in test tubes, to transfection of neurons in cell culture Detailed recommendations on where to find protocols and other resources for specific techniques "Walk-through" boxes that guide readers through experiments step-by-step

Download or read book The Neuronal Ceroid Lipofuscinoses Batten Disease written by Sara Mole and published by OUP Oxford. This book was released on 2011-03-10 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: The neuronal ceroid lipofuscinoses are an extremely rare group of inherited neurodegenerative diseases that primarily affect children. Core symptoms of these conditions typically include epilepsy, cognitive decline and visual failure. These diseases are so rare that professionals who come into contact with them need a consultative reference work that enables them to become expert, or identify who to contact for more details. Fully updated and revised, this second edition continues to be the definitive volume on this devastating group of disorders. Written by an international collection of authorities in the field, it provides invaluable advice on their diagnosis, patient care, and new treatments that are available. This new edition of the definitive reference text on the neuronal ceroid lipofuscinoses will prove useful for clinicians, family physicians, research scientists, diagnostic laboratories, families affected by the disease as well as by workers in industry planning translational research.

Download or read book D sir Collen Biotech Pioneer written by Paul Huybrechts and published by Désiré Collen. This book was released on 2020-08-27 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: Désiré Collen, Biotech Pioneer relates the fascinating story of scientific discovery in a time when biotechnology was not yet a science. Although the cultivation and cross fertilization of plants were, strictly speaking, biotechnological techniques, modern biotechnology dates from the early 1970s, when pioneers such as biochemist Herbert Boyer from the university of California managed to transfer genetic material into a bacterium. Together with venture capitalist Robert Swanson, Boyer set up Genentech, one of the first genetic engineering companies. Just a few years later, on the other side of the Atlantic, in Leuven, Désiré Collen discovered t-PA, the enzyme responsible for fibrinolysis, or the dissolving of blood clots. Clogged arteries were then still one of the major causes of death. The ensuing cooperation between Collen and Genentech was the beginning of a long-lasting success story, from which not only Collen but also scientific research and the University of Leuven benefitted greatly for many years. According to a Reuters ranking, KU Leuven has been, from 2016 onwards, the most innovative university in Europe. Flanders and Belgium served as the cradle of several highly successful biotech companies. t-PA was a relatively expensive medicine, and Collen went on to develop a much cheaper clot-dissolving remedy to benefit patients in less affluent countries. He failed, however, to find the necessary finances for Phase 3 trials. Meanwhile, he had set up ThromboGenics, a company which later specialized in ophthalmology. Collen continued to stimulate and finance research in other fields, such as the cardiovascular research of Peter Carmeliet. In 2013 he left ThromboGenics, following a difference in views on the company’s focus, and in 2015 he set up Fund+, a biotech-oriented investment firm. Fund+ has meanwhile acquired a prominent place among European biotech investment funds and has scored some astonishing early successes. In June 2020, Fund+ had 13 companies in its portfolio, with several more waiting to come on board.

Download or read book Accelerando written by Charles Stross and published by Penguin. This book was released on 2005-07-05 with total page 596 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Singularity. It is the era of the posthuman. Artificial intelligences have surpassed the limits of human intellect. Biotechnological beings have rendered people all but extinct. Molecular nanotechnology runs rampant, replicating and reprogramming at will. Contact with extraterrestrial life grows more imminent with each new day. Struggling to survive and thrive in this accelerated world are three generations of the Macx clan: Manfred, an entrepreneur dealing in intelligence amplification technology whose mind is divided between his physical environment and the Internet; his daughter, Amber, on the run from her domineering mother, seeking her fortune in the outer system as an indentured astronaut; and Sirhan, Amber’s son, who finds his destiny linked to the fate of all of humanity. For something is systematically dismantling the nine planets of the solar system. Something beyond human comprehension. Something that has no use for biological life in any form...

Download or read book Visualizing RNA Dynamics in the Cell written by and published by Academic Press. This book was released on 2016-05-27 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Enzymology: Visualizing RNA Dynamics in the Cell continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods visualizing RNA dynamics in the cell, and includes sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2, and visualization of 5S dynamics in live cells using photostable corn probe. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in visualizing RNA dynamics in the cell Contains sections on such topics as identification of RNA cis-regulatory sequences, IRAS, IMAGEtags, MERFISH, plant RNA labeling using MS2 and visualization of 5S dynamics in live cells using photostable corn probe

Download or read book Elements of Molecular Neurobiology written by C. U. M. Smith and published by John Wiley & Sons. This book was released on 2003-06-13 with total page 630 pages. Available in PDF, EPUB and Kindle. Book excerpt: This edition of the popular text incorporates recent advances in neurobiology enabled by modern molecular biology techniques. Understanding how the brain works from a molecular level allows research to better understand behaviours, cognition, and neuropathologies. Since the appearance six years ago of the second edition, much more has been learned about the molecular biology of development and its relations with early evolution. This "evodevo" (as it has come to be known) framework also has a great deal of bearing on our understanding of neuropathologies as dysfunction of early onset genes can cause neurodegeneration in later life. Advances in our understanding of the genomes and proteomes of a number of organisms also greatly influence our understanding of neurobiology. * Well known and widely used as a text throughout the UK, good reviews from students and lecturers. * Good complement to Fundementals of Psychopharmacology by Brian Leonard. This book will be of particular interest to biomedical undergraduates undertaking a neuroscience unit, neuroscience postgraduates, physiologists, pharmacologists. It is also a useful basic reference for university libraries. Maurice Elphick, Queen Mary, University of London "I do like this book and it is the recommended textbook for my course in Molecular Neuroscience. The major strength of the book is the overall simplicity of the format both in terms of layout and diagrams."