Download or read book Single Molecule Detection and Fluorescence Correlation Spectroscopy on Surfaces written by Kai Hassler and published by . This book was released on 2006 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Single Molecule Detection in Solution written by Christoph Zander and published by Wiley-VCH. This book was released on 2002-05-06 with total page 398 pages. Available in PDF, EPUB and Kindle. Book excerpt: The detection of single molecules opens up new horizons in analytical chemistry, biology and medicine. This discipline, which belongs to the expanding field of nanoscience, has been rapidly emerging over the last ten years. This handbook provides a thorough overview of the field. It begins with basics of single molecule detection in solution, describes methods and devices (fluorescense correlation spectroscopy, surface enhanced Raman scattering, sensors, especially dyes, screening techniques, especially confocal laser scanning microscopy). In the second part, various applications in life sciences and medicine provide the latest research results. This modern handbook is a highly accessible reference for a broad community from advanced researchers, specialists and company professionals in physics, spectroscopy, biotechnology, analytical chemistry, and medicine. Written by leading authorities in the field, it is timely and fills a gap - up to now there exists no handbook concerning this theme.
Download or read book Single Molecule Spectroscopy written by R. Rigler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: The topics range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.
Download or read book Fluorescence Correlation Spectroscopy written by R. Rigler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 503 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the first book-length treatment of both the theoretical background to fluorescence correlation spectroscopy (FCS) and a variety of applications in various fields of science. The high spatial and temporal resolution of FCS has made it a powerful tool for the analysis of molecular interactions and kinetics, transport properties due to thermal motion, and flow. It contains an essential contribution from Nobel Prize winner M. Eigen, who is credited with inventing FCS.
Download or read book Time Resolved Single Molecule Fluorescence Spectroscopy on Surface Tethered and Freely Diffusing Proteins written by Diaa Atta and published by Forschungszentrum Jülich. This book was released on 2012 with total page 143 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Applications of Single Molecule Fluorescence Detection written by Jennifer Lynn Glass and published by . This book was released on 2000 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Download or read book Handbook of Single Molecule Fluorescence Spectroscopy written by Chris Gell and published by Oxford University Press on Demand. This book was released on 2006-08-17 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a practical introduction to single molecule fluorescence experiments, the analysis of the data, and applications of the techniques to the study of biological structure and function.
Download or read book Single Molecule Tools Part A Fluorescence Based Approaches written by and published by Academic Press. This book was released on 2010-08-17 with total page 559 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. - Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools - Methods included are useful to both established researchers and newcomers to the field - Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines
Download or read book Spectroscopy and Dynamics of Single Molecules written by and published by Elsevier. This book was released on 2019-08-14 with total page 404 pages. Available in PDF, EPUB and Kindle. Book excerpt: Spectroscopy and Dynamics of Single Molecules: Methods and Applications reviews the most recent developments in spectroscopic methods and applications. Spectroscopic techniques are the chief experimental methods for testing theoretical models and research in this area plays an important role in stimulating new theoretical developments in physical chemistry. This book provides an authoritative insight into the latest advances in the field, highlighting new techniques, current applications, and potential future developments An ideal reference for chemists and physicists alike, Spectroscopy and Dynamics of Single Molecules: Methods and Applications is a useful guide for all those working in the research, design, or application of spectroscopic tools and techniques across a wide range of fields. - Includes the latest research on ultrafast vibrational and electronic dynamics, nonlinear spectroscopies, and single-molecule methods - Makes the content accessible to researchers in chemistry, biophysics, and chemical physics through a rigorous multi-disciplinary approach - Provides content edited by a world-renowned chemist with more than 30 years of experience in research and instruction
Download or read book Single Molecule Spectroscopy in Chemistry Physics and Biology written by Astrid Graslund and published by Springer Science & Business Media. This book was released on 2010-04-17 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by the leading experts in the field, this book describes the development and current state of the art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.
Download or read book Handbook of Fluorescence Spectroscopy and Imaging written by Markus Sauer and published by John Wiley & Sons. This book was released on 2010-12-23 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap. Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging. Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.
Download or read book Ultrasensitive and Single molecule Detection Technologies written by Jörg Enderlein and published by SPIE-International Society for Optical Engineering. This book was released on 2006 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proceedings of SPIE present the original research papers presented at SPIE conferences and other high-quality conferences in the broad-ranging fields of optics and photonics. These books provide prompt access to the latest innovations in research and technology in their respective fields. Proceedings of SPIE are among the most cited references in patent literature.
Download or read book Monte Carlo Simulations and Analysis of Single molecule Detection and Imaging written by and published by . This book was released on 2003 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Computer modeling and analysis methods are developed for two modes of operation of an instrument for sensitive fluorescence detection of individual dye-labeled molecules in solution. First, Monte Carlo simulations of experiments for single-molecule imaging (SMI) are extended to include effects of sample flow, sticking of molecules to surfaces, and the finite depth-of-focus of the optics. The results have a bearing on a patented method for high-speed single-molecule DNA sequencing. They indicate that the imaging of freely moving fluorescent labels within a microfluidic flowcell will be considerably more involved than that of immobilized molecules at a surface, which is the usual situation in SMI experiments. Second, the detection of single molecules as they pass through a tightly focused laser beam is discussed, with an emphasis on fluorescence correlation spectroscopy and the analysis of the autocorrelation function of the photon counts. Analysis methods are developed and applied to data from a collaborative experimental study within the topic of RNA transcription. The methods are extended to the case of flowing solution, for ongoing research with application to high-throughput pharmaceutical drug screening.
Download or read book Single Molecule Optical Detection Imaging and Spectroscopy written by W. E. Moerner and published by John Wiley & Sons. This book was released on 2008-09-26 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single Molecule Spectroscopy is one of the hottest topics in today's chemistry. It brings us close to the the most exciting vision generations of chemists have been dreaming of: To observe and examine single molecules! While most of chemistry deals with myriads of molecules, this books presents the latest developments for the detection and investigation of single entities. Written by internationally renowned authors, it is a thorough and comprehensive survey of current methods and their applications.
Download or read book Single Molecule Imaging with a Custom built Fluorescence Microscope written by and published by . This book was released on 2002 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: An apparatus for single-molecule imaging was constructed and used to examine nanomolar solutions of Bodipy Texas Red in water. The illumination source for this microscope is a synchronously pumped dye laser operating at a wavelength of 585 nm. The beam can easily be switched to give total internal reflection or wide field epi-illumination excitation of the sample. The instrument also has the capability of being used for single-molecule detection by confocal epiillumination and for fluorescence correlation spectroscopy. Movies of single molecules were analyzed using custom software written in Matlab. Molecules stuck to the surface revealed fluctuations in intensity, or blinking, for both forms of illumination. Possible causes for the fluctuations are discussed.
Download or read book Single Biomolecule Detection and Analysis written by Tuhin Subhra Santra and published by CRC Press. This book was released on 2023-08-15 with total page 355 pages. Available in PDF, EPUB and Kindle. Book excerpt: This collection discusses various micro/nanodevice design and fabrication for single-biomolecules detection. It will be an ideal reference text for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience. This book- Discusses techniques of single-biomolecule detection, their advantages, limitations, and applications. Covers comprehensively several electrochemical detection techniques. Provides single-molecule separation, sensing, imaging, sequencing, and analysis in detail. Examines different types of cantilever-based biomolecule sensing, and its limitations. Single Biomolecule Detection and Analysis covers single-biomolecule detection and characterization using micro/nanotechnologies and micro/nanofluidic devices, electrical and magnetic detection technologies, microscopy and spectroscopy techniques, single biomolecule optical, and nanopore devices. The text covers key important biosensors-based detection, stochastic optical reconstruction microscopy-based detection, electrochemical detection, metabolic engineering of animal cells, single-molecule intracellular delivery and tracking, terahertz spectroscopy-based detection, total internal reflection fluorescence (TIFR) detection, and Fluorescence Correlation Spectroscopy (FCS) detection. The text will be useful for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience. Discussing chemical process, physical process, separation, sensing, imaging, sequencing, and analysis of single-molecule detection, this text will be useful for graduate students and professionals in diverse subject areas including materials science, biomedical engineering, chemical engineering, mechanical engineering, and nanoscience. It covers microscopy and spectroscopy techniques for single-biomolecule detection, analysis, and their biomedical engineering applications.
Download or read book Fluorescence Spectroscopy Imaging and Probes written by Ruud Kraayenhof and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: The increased use of fluorescence techniques is greatly enhanced by the improved instrumentation pioneered by inventive scientists and now made available commercially by several high-tech companies. Moreover, the design and development of many new molecular probes with higher selectivity for specific microenvironmental properties has stimulated many new researchers to employ fluorescence techniques for solving their problems. This topic book, the second in his series, reflects this exciting scientific progress and deals, among others, with new approaches and new probes in fluorescence spectroscopy, single molecule fluorescence, applications in biomembrane and enzyme studies and imaging of living cells.
